Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: HPLC-ELSD chromatogram of the crude extract of Micromonospora sp. 28ISP2-46 T .

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Phylogenetic tree for strain 28ISP2-46 T and the other group IA Micromonospora species generated using the TYGS and drawn with iTOL. The tree was constructed using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values from 100 replications, with an average branch support of 98.4% and a delta statistic of 0.135. The tree was rooted using Micromonospora pallida as the outgroup.

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques: Generated, Construct

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: Biosynthetic gene clusters identified in the genome of strain 28ISP2-46 T using AntiSMASH.

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques:

Journal: Marine Drugs

Article Title: A New Micromonospora Strain with Antibiotic Activity Isolated from the Microbiome of a Mid-Atlantic Deep-Sea Sponge

doi: 10.3390/md19020105

Figure Lengend Snippet: ( a ) ClusterBLAST hits for the kosinostatin cluster. Homologues of kst genes are coloured as follows: kstA genes red, kstB genes yellow, kstC genes green, kstD genes blue, kstRg genes black, kstRs genes white, and genes uninvolved in kosinostatin biosynthesis grey. A black line at the end of a cluster indicates that it is close to the end of a contig. Gene sizes not to scale. The NCBI protein IDs for first and last coloured genes of each cluster are as follows: Micromonospora sp. TP-A0468 AFJ52719.1 and AFJ52701.1; Micromonospora sp. 28ISP2-46 T QLQ36639.1 and QLQ36600.1; N. valliformis WP_017579213.1 and WP_017579258.1; N. alkaliphila WP_017604198.1 and WP_017604153.1; Streptomyces sp. SM8 PKA38649.1 and PKA38729.1; Streptomyces sp. ScaeMP-6W SCE31237.1 and SCE31955.1; A. bangkokensis OLR89622.1 and OLR89598.1; M. haikouensis SCF11188.1 and SCF11278.1. ( b ) The same gene clusters rearranged for ease of comparison. Coloured as 6a.

Article Snippet: 16S rRNA gene and whole-genome sequencing of strain 28ISP2-46 T was consistent with its classification as a novel species of the genus Micromonospora , for which the name Micromonospora ferruginea sp. nov. (NCTC number: 14469 T , DSMZ number: 111791 T ) is proposed.

Techniques:

Journal:

Article Title: Stable Carbon Isotope Ratios of Lipid Biomarkers of Sulfate-Reducing Bacteria

doi: 10.1128/AEM.70.2.745-751.2004

Figure Lengend Snippet: Characteristics of SRB used in culture study

Article Snippet: Desulfobacterium autotrophicum (ATCC 43914 ) , Desulfobacter hydrogenophilus (ATCC 43915), Desulfovibrio desulfuricans (ATCC 29577), and Desulfotomaculum acetoxidans (ATCC 49208) were obtained from the American Type Culture Collection.

Techniques: Staining, Biomarker Assay

Journal:

Article Title: Stable Carbon Isotope Ratios of Lipid Biomarkers of Sulfate-Reducing Bacteria

doi: 10.1128/AEM.70.2.745-751.2004

Figure Lengend Snippet: Comparison of FAME compositions of four SRB grown under two conditions d

Article Snippet: Desulfobacterium autotrophicum (ATCC 43914 ) , Desulfobacter hydrogenophilus (ATCC 43915), Desulfovibrio desulfuricans (ATCC 29577), and Desulfotomaculum acetoxidans (ATCC 49208) were obtained from the American Type Culture Collection.

Techniques:

Journal:

Article Title: Stable Carbon Isotope Ratios of Lipid Biomarkers of Sulfate-Reducing Bacteria

doi: 10.1128/AEM.70.2.745-751.2004

Figure Lengend Snippet: Δδ13C between FA and biomass of Desulfovibrio desulfuricans grown mixotrophically with CO2 plus acetate (black) or heterotrophically with lactate (gray) (A); Desulfotomaculum acetoxidans grown autotrophically with CO2 (black) or heterotrophically with acetate (gray) (B); Desulfobacter hydrogenophilus grown autotrophically with CO2 (black) or heterotrophically with acetate (gray) (C); and Desulfobacterium autotrophicum grown autotrophically with CO2 (black) or heterotrophically with acetate (gray) (D). Values are averages of three analyses each of duplicate cultures, and error bars indicate ±1 standard deviation.

Article Snippet: Desulfobacterium autotrophicum (ATCC 43914 ) , Desulfobacter hydrogenophilus (ATCC 43915), Desulfovibrio desulfuricans (ATCC 29577), and Desulfotomaculum acetoxidans (ATCC 49208) were obtained from the American Type Culture Collection.

Techniques: Standard Deviation

Journal:

Article Title: Stable Carbon Isotope Ratios of Lipid Biomarkers of Sulfate-Reducing Bacteria

doi: 10.1128/AEM.70.2.745-751.2004

Figure Lengend Snippet: δ 13 C of biomass and FA of four SRB under two different growth conditions

Article Snippet: Desulfobacterium autotrophicum (ATCC 43914 ) , Desulfobacter hydrogenophilus (ATCC 43915), Desulfovibrio desulfuricans (ATCC 29577), and Desulfotomaculum acetoxidans (ATCC 49208) were obtained from the American Type Culture Collection.

Techniques: