serratia entomophila  (ATCC)


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    ATCC serratia entomophila
    Serratia Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    serratia entomophila  (ATCC)


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    ATCC serratia entomophila
    Serratia Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    serratia entomophila  (ATCC)


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    ATCC serratia entomophila
    Classification by MALDI-MS biotyping of Micrococcus luteus ( M. l. ) and Pectobacterium caratovorum subsp. caratovorum, (P. c. c.) , Serratia <t>entomophila</t> , ( S. e. ) bacteria including the Serratia marcescens ( S. m .) isolated from a naturally infected bee. The strains used to build the infectious model ( M. l. and P. c.c. ) and to assess the computational capability to discriminate proteomic fingerprints from the same bacterial species ( S. m. and S. e. ). were analyzed in order to confirm their identity based on their molecular profiles by matching the MALDI MS spectra (mass range m / z 2,000 to 11,000) to the reference strains of the Bruker database containing 6,903 MSP. The BioTyper parameters to validate the identifications of strains, i.e. the scores and the matching strains (references and library), were obtained for each biotyped bacterial sample in addition to the MALDI MS spectral gel-view. The dendrogram was built from the Main Spectra Projection (MSP) statistical mode of calculation, which is used to identify, analyze and classify the MALDI MS spectra. These bacteria were identified by their scores and classified according to their distance level (MSP Dendogram) in comparison to reference strains from the database MBT Compass 4.1, build 70 ( M. l . IMET 11249HKJ, P. c. ssp odoriferum NB 1892 PAH, S. m. 13103_1 CHB and S. e. DSM 12358T DSM). The mass spectra (m / z ) were transformed into gel views where the grey scale bar and thickness of the lines refer to the m / z peak intensities. Classically, a high confidence identification is obtained with a score between 2.00 and 3.00, a low confidence identification with a score between 1.70 and 1.99 and a failed identification with a score strictly below 1.70. The mass spectra (m/z) were transformed into gel views where the grey scale bar and thickness of the lines refer to the m/z peak intensities.
    Serratia Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A MALDI-MS biotyping-like method to address honey bee health status through computational modelling"

    Article Title: A MALDI-MS biotyping-like method to address honey bee health status through computational modelling

    Journal: bioRxiv

    doi: 10.1101/683607

    Classification by MALDI-MS biotyping of Micrococcus luteus ( M. l. ) and Pectobacterium caratovorum subsp. caratovorum, (P. c. c.) , Serratia entomophila , ( S. e. ) bacteria including the Serratia marcescens ( S. m .) isolated from a naturally infected bee. The strains used to build the infectious model ( M. l. and P. c.c. ) and to assess the computational capability to discriminate proteomic fingerprints from the same bacterial species ( S. m. and S. e. ). were analyzed in order to confirm their identity based on their molecular profiles by matching the MALDI MS spectra (mass range m / z 2,000 to 11,000) to the reference strains of the Bruker database containing 6,903 MSP. The BioTyper parameters to validate the identifications of strains, i.e. the scores and the matching strains (references and library), were obtained for each biotyped bacterial sample in addition to the MALDI MS spectral gel-view. The dendrogram was built from the Main Spectra Projection (MSP) statistical mode of calculation, which is used to identify, analyze and classify the MALDI MS spectra. These bacteria were identified by their scores and classified according to their distance level (MSP Dendogram) in comparison to reference strains from the database MBT Compass 4.1, build 70 ( M. l . IMET 11249HKJ, P. c. ssp odoriferum NB 1892 PAH, S. m. 13103_1 CHB and S. e. DSM 12358T DSM). The mass spectra (m / z ) were transformed into gel views where the grey scale bar and thickness of the lines refer to the m / z peak intensities. Classically, a high confidence identification is obtained with a score between 2.00 and 3.00, a low confidence identification with a score between 1.70 and 1.99 and a failed identification with a score strictly below 1.70. The mass spectra (m/z) were transformed into gel views where the grey scale bar and thickness of the lines refer to the m/z peak intensities.
    Figure Legend Snippet: Classification by MALDI-MS biotyping of Micrococcus luteus ( M. l. ) and Pectobacterium caratovorum subsp. caratovorum, (P. c. c.) , Serratia entomophila , ( S. e. ) bacteria including the Serratia marcescens ( S. m .) isolated from a naturally infected bee. The strains used to build the infectious model ( M. l. and P. c.c. ) and to assess the computational capability to discriminate proteomic fingerprints from the same bacterial species ( S. m. and S. e. ). were analyzed in order to confirm their identity based on their molecular profiles by matching the MALDI MS spectra (mass range m / z 2,000 to 11,000) to the reference strains of the Bruker database containing 6,903 MSP. The BioTyper parameters to validate the identifications of strains, i.e. the scores and the matching strains (references and library), were obtained for each biotyped bacterial sample in addition to the MALDI MS spectral gel-view. The dendrogram was built from the Main Spectra Projection (MSP) statistical mode of calculation, which is used to identify, analyze and classify the MALDI MS spectra. These bacteria were identified by their scores and classified according to their distance level (MSP Dendogram) in comparison to reference strains from the database MBT Compass 4.1, build 70 ( M. l . IMET 11249HKJ, P. c. ssp odoriferum NB 1892 PAH, S. m. 13103_1 CHB and S. e. DSM 12358T DSM). The mass spectra (m / z ) were transformed into gel views where the grey scale bar and thickness of the lines refer to the m / z peak intensities. Classically, a high confidence identification is obtained with a score between 2.00 and 3.00, a low confidence identification with a score between 1.70 and 1.99 and a failed identification with a score strictly below 1.70. The mass spectra (m/z) were transformed into gel views where the grey scale bar and thickness of the lines refer to the m/z peak intensities.

    Techniques Used: Isolation, Infection, Transformation Assay

    Differential PCA-based analysis of hemolymph fingerprints following infection with two Serratia strains, Serratia marcescens ( S. m. ) isolated from a naturally infected Apis mellifera and S. entomophila ( S. e. ) and statistical relevance of predictive markers. The differential fingerprinting and PCA analysis discriminated the non-experimentally infected bees (control in black) and the experimentally infected groups ( S. e. in red or S. m . in green) with n=13 bees per group ( A ). The detected peaks in the differential analysis with the highest and lowest discriminant scores were assessed statistically by measuring the standard deviation and the 95% confidence interval. ( B ). The most interesting peaks classified through the Receiver Operating Characteristics (ROC) curves are shown using the Area Under Curve (AUC) calculation ( C ). The biological model used as the positive class was the experimental S. m. infection and the sensibility (True Positives) and specificity (False Positives) parameters were determined for all calculated peaks. Eight markers defined by their m / z values (Da) were found as the best predictive markers (AUC>0.8) for discriminating honey bee infections ( S. e. or S. m. ). Conversely, the two least discriminant peaks had an irrelevant AUC (◻ 0.5) with ROC curves fitting the non-discriminant line of the statistical test ( D ).
    Figure Legend Snippet: Differential PCA-based analysis of hemolymph fingerprints following infection with two Serratia strains, Serratia marcescens ( S. m. ) isolated from a naturally infected Apis mellifera and S. entomophila ( S. e. ) and statistical relevance of predictive markers. The differential fingerprinting and PCA analysis discriminated the non-experimentally infected bees (control in black) and the experimentally infected groups ( S. e. in red or S. m . in green) with n=13 bees per group ( A ). The detected peaks in the differential analysis with the highest and lowest discriminant scores were assessed statistically by measuring the standard deviation and the 95% confidence interval. ( B ). The most interesting peaks classified through the Receiver Operating Characteristics (ROC) curves are shown using the Area Under Curve (AUC) calculation ( C ). The biological model used as the positive class was the experimental S. m. infection and the sensibility (True Positives) and specificity (False Positives) parameters were determined for all calculated peaks. Eight markers defined by their m / z values (Da) were found as the best predictive markers (AUC>0.8) for discriminating honey bee infections ( S. e. or S. m. ). Conversely, the two least discriminant peaks had an irrelevant AUC (◻ 0.5) with ROC curves fitting the non-discriminant line of the statistical test ( D ).

    Techniques Used: Infection, Isolation, Standard Deviation

    serratia entomophila  (ATCC)


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    ATCC serratia entomophila
    Abilities of Colilert and MI agar culture-based methods as well as lacZ , wecG , and 16S rRNA PCR assays to detect total coliforms
    Serratia Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Molecular Method for Detection of Total Coliforms in Drinking Water Samples"

    Article Title: Molecular Method for Detection of Total Coliforms in Drinking Water Samples

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00546-14

    Abilities of Colilert and MI agar culture-based methods as well as lacZ , wecG , and 16S rRNA PCR assays to detect total coliforms
    Figure Legend Snippet: Abilities of Colilert and MI agar culture-based methods as well as lacZ , wecG , and 16S rRNA PCR assays to detect total coliforms

    Techniques Used:

    p entomophila  (ATCC)


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    ATCC p entomophila
    P Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    serratia entomophila  (ATCC)


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    ATCC serratia entomophila
    Serratia Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    serratia entomophila strain a1mo2  (ATCC)


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    ATCC serratia entomophila strain a1mo2
    Serratia Entomophila Strain A1mo2, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    serratia entomophila  (ATCC)


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    ATCC serratia entomophila
    Specificity of the Y. enterocolitica TaqMan assay a
    Serratia Entomophila, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Rapid Identification of Yersinia enterocolitica in Blood by the 5? Nuclease PCR Assay"

    Article Title: Rapid Identification of Yersinia enterocolitica in Blood by the 5? Nuclease PCR Assay

    Journal:

    doi:

    Specificity of the Y. enterocolitica TaqMan assay a
    Figure Legend Snippet: Specificity of the Y. enterocolitica TaqMan assay a

    Techniques Used: TaqMan Assay

    probe 45 serratia entomophila atcc  (ATCC)


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    ATCC probe 45 serratia entomophila atcc
    Probe 45 Serratia Entomophila Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    probe 45 serratia entomophila atcc  (ATCC)


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    ATCC probe 45 serratia entomophila atcc
    Probe 45 Serratia Entomophila Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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