Journal: Pathogens

Article Title: Methylobacterium spp. as Emerging Opportunistic Premise Plumbing Pathogens

doi: 10.3390/pathogens9020149

Figure Lengend Snippet: Persistence of Methylobacterium spp. strains in tap water.

Article Snippet: M. extorquens , ATCC 43645 , Soil, Japan , Yes.

Techniques:

Journal: Pathogens

Article Title: Methylobacterium spp. as Emerging Opportunistic Premise Plumbing Pathogens

doi: 10.3390/pathogens9020149

Figure Lengend Snippet: Contact angles (degrees) of Methylobacterium spp. and Escherichia coli cells on different plumbing surfaces *.

Article Snippet: M. extorquens , ATCC 43645 , Soil, Japan , Yes.

Techniques:

Journal: Pathogens

Article Title: Methylobacterium spp. as Emerging Opportunistic Premise Plumbing Pathogens

doi: 10.3390/pathogens9020149

Figure Lengend Snippet: Adherence of Methylobacterium extorquens strain ATCC 43645 cells to plumbing surfaces *.

Article Snippet: M. extorquens , ATCC 43645 , Soil, Japan , Yes.

Techniques:

Journal: Pathogens

Article Title: Methylobacterium spp. as Emerging Opportunistic Premise Plumbing Pathogens

doi: 10.3390/pathogens9020149

Figure Lengend Snippet: Biofilm formation on plumbing surfaces by M. extorquens .

Article Snippet: M. extorquens , ATCC 43645 , Soil, Japan , Yes.

Techniques:

Journal: Pathogens

Article Title: Methylobacterium spp. as Emerging Opportunistic Premise Plumbing Pathogens

doi: 10.3390/pathogens9020149

Figure Lengend Snippet: Survival of Methylobacterium spp. strains at 60 °C.

Article Snippet: M. extorquens , ATCC 43645 , Soil, Japan , Yes.

Techniques:

Journal: Pathogens

Article Title: Methylobacterium spp. as Emerging Opportunistic Premise Plumbing Pathogens

doi: 10.3390/pathogens9020149

Figure Lengend Snippet: Methylobacterium spp. strains used in this study.

Article Snippet: M. extorquens , ATCC 43645 , Soil, Japan , Yes.

Techniques:

Journal: Pathogens

Article Title: Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.

doi: 10.3390/pathogens6030042

Figure Lengend Snippet: Adherence of M. avium strain A5 to stainless steel coupons after 6 h in the presence and absence of established normal tap water and Methylobacterium spp. biofilms measured by colony counts (±standard error).

Article Snippet: Consortium 1 or 2/mL; (3) a water-acclimated suspension of 10 5 CFU Philadelphia yeast isolate P32-14/mL; (4) a water-acclimated suspension of 10 5 D. grandis strain JM-1-1/mL, or a water-acclimated suspension of 10 5 M. avium strain A5 (for measurement of M. extorquens strain ATCC 43645 adherence to M. avium biofilms).

Techniques:

Journal: Pathogens

Article Title: Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.

doi: 10.3390/pathogens6030042

Figure Lengend Snippet: Mycobacterium avium strain A5 Adherence to Biofilms of Deinococcus grandis strain JM-1-1 and Yeast strain 32-14 1 .

Article Snippet: Consortium 1 or 2/mL; (3) a water-acclimated suspension of 10 5 CFU Philadelphia yeast isolate P32-14/mL; (4) a water-acclimated suspension of 10 5 D. grandis strain JM-1-1/mL, or a water-acclimated suspension of 10 5 M. avium strain A5 (for measurement of M. extorquens strain ATCC 43645 adherence to M. avium biofilms).

Techniques:

Journal: Pathogens

Article Title: Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.

doi: 10.3390/pathogens6030042

Figure Lengend Snippet: Adherence of M. avium strain A5 to stainless steel coupons in the presence and absence of Methylobacterium spp. biofilms measured by qPCR 1,2 .

Article Snippet: Consortium 1 or 2/mL; (3) a water-acclimated suspension of 10 5 CFU Philadelphia yeast isolate P32-14/mL; (4) a water-acclimated suspension of 10 5 D. grandis strain JM-1-1/mL, or a water-acclimated suspension of 10 5 M. avium strain A5 (for measurement of M. extorquens strain ATCC 43645 adherence to M. avium biofilms).

Techniques:

Journal: Pathogens

Article Title: Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.

doi: 10.3390/pathogens6030042

Figure Lengend Snippet: Survival of M. avium strain A5 cells adhering to Consortium 1 and 2 biofilms 1 .

Article Snippet: Consortium 1 or 2/mL; (3) a water-acclimated suspension of 10 5 CFU Philadelphia yeast isolate P32-14/mL; (4) a water-acclimated suspension of 10 5 D. grandis strain JM-1-1/mL, or a water-acclimated suspension of 10 5 M. avium strain A5 (for measurement of M. extorquens strain ATCC 43645 adherence to M. avium biofilms).

Techniques:

Journal: Pathogens

Article Title: Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.

doi: 10.3390/pathogens6030042

Figure Lengend Snippet: M. avium strain A5 biofilms inhibit the adherence of Methylobacterium spp. cells to stainless steel 1 .

Article Snippet: Consortium 1 or 2/mL; (3) a water-acclimated suspension of 10 5 CFU Philadelphia yeast isolate P32-14/mL; (4) a water-acclimated suspension of 10 5 D. grandis strain JM-1-1/mL, or a water-acclimated suspension of 10 5 M. avium strain A5 (for measurement of M. extorquens strain ATCC 43645 adherence to M. avium biofilms).

Techniques:

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques: Plasmid Preparation

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques: Construct

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques:

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: Cytokinins recovered from supernatants of free-living PPFM cultures

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques:

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques: Purification

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques: Isolation, Mutagenesis

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.

Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

Techniques: Mutagenesis, Isolation

Journal:

Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

doi: 10.1128/JB.184.7.1832-1842.2002

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: M. extorquens ATCC 43645 , Type strain , ATCC a.

Techniques: Plasmid Preparation