acidomonas methanolica atcc 43582  (ATCC)

Journal: Scientific Reports

Article Title: Distribution and genome structures of temperate phages in acetic acid bacteria

doi: 10.1038/s41598-021-00998-w

Figure Lengend Snippet: Morphological characteristics of AAB temperate phages.

Article Snippet: Acidomonas methanolica ATCC 43582 , Ditch sludge , 44.4 (0.0) , 75.3 (1.7) , 3 , -.

Techniques: Isolation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: Effects of 14–3-3η on antioxidation in HCC cells: a qRT-PCT analyses in triplicate and Western blot analyses of the expressions of 14–3-3η mRNA (top) and protein (bottom). b and c Bel/5-Fu cells were transfected by scrambled or 14–3-3η-siRNA. d and e After Bel-7402 cells were transfected by scrambled or 14–3-3η-Flag, they were exposed to 0 or 500 μM hydrogen peroxide for 24 h. b and d The intracellular ROS levels; c and e Cell viability (determined in triplicate)

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: Western Blot, Transfection

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: Effects of 14–3-3η on MDR in HCC cells: a to c Bel/5-Fu cells were transfected by scrambled or 14–3-3η-siRNA. d to f Bel-7402 cells were transfected by scrambled or 14–3-3η-Flag. Cells were treated by different concentrations of 5-fluorouracil, oxaliplatin, or doxorubicin for 24 h. The cell viability was determined in triplicate, and the IC 50s were calculated

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: Transfection

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: Effects of NF-κB on 14–3-3η and MDR in HCC cells: a Bel/5-Fu cells were treated by 20 μM of ML-385, topotecan, stattic, or BAY-117082 for 24 h. qRT-PCT analyses in triplicate of the 14–3-3η mRNA. b to e Bel/5-Fu cells were transfected by scrambled or NF-κB/p65-siRNA. b qRT-PCT analyses in triplicate of the NF-κB/p65 and 14–3-3η mRNAs. c , top Western blot analyses of the expressions of p-p65 and 14–3-3η. c , bottom) Southwestern blot analyses of the binding of probe (containing the κB sequence located in 14–3-3η promoter) to NF-κB/p65. d Densitometric analysis. e The cell viability was determined in triplicate, and the IC 50s were calculated

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: Transfection, Western Blot, Southwestern Blot, Binding Assay, Sequencing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: Effects of 14–3-3η on NF-κB in HCC cells: a to c Bel/5-Fu cells were transfected by scrambled or 14–3-3η-siRNA. d to f Bel-7402 cells were transfected by scrambled or 14–3-3η-Flag. a and d Western blot analyses of the expressions of p-IKKβ, p-IκBα, p-p65 and 14–3-3η/Flag. b and e Densitometric analysis. c and f Immunofluorescence analyses of the intracellular translocation of NF-κB/p65

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: Transfection, Western Blot, Immunofluorescence, Translocation Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: Relationships between ATO and 14–3-3η: a Bel/5-Fu cells were treated by 10 μM of ATO for 24 h. qRT-PCT analyses in triplicate (left) and Western blot (right) analyses of the expressions of 14–3-3η and/or p-p65. b PyMol software analyses of the binding of ATO to 14–3-3η. c The procedure of IP-AFS. d and e After Bel/5-Fu cells were pre-treated by 20 μM of MG-132 for 2 h, they were exposed to 10 μM of ATO for 6 h. The 14–3-3η was immunoprecipitated with the specific antibody. d AFS analyses of the concentration of ATO in 14–3-3η-immunoprecipitation complex. e Western blot analyses of the expression of ubiquitin in 14–3-3η-immunoprecipitation complex

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: Western Blot, Software, Binding Assay, Immunoprecipitation, Concentration Assay, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: 14–3-3η was involved in ATO-reversed MDR: The ( a to c ) scramble- or ( d to f ) 14–3-3η-siRNA transfected Bel/5-Fu cells were treated by different concentrations of 5-fluorouracil, oxaliplatin, and doxorubicin, in the absence of presence of 10 μM ATO for 24 h. The cell viability was determined in triplicate, and the IC50s were calculated

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: Transfection

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Arsenic trioxide reverses the chemoresistance in hepatocellular carcinoma: a targeted intervention of 14–3-3η/NF-κB feedback loop

doi: 10.1186/s13046-018-1005-y

Figure Lengend Snippet: Arsenic Trioxide Reverses the Chemoresistance in Hepatocellular Carcinoma: A Targeted Intervention of 14–3-3η/NF-κB Feedback Loop

Article Snippet: Scrambled and pcDNA-3.1-14-3-3η-Flag were synthesized by Generay Biotech (Shanghai, China); while 14–3-3η- and NF-κB/p65 siRNA were purchased from Santa Cruz Biotechnology ( http://datasheets.scbt.com/sc-43581.pdf , and https://datasheets.scbt.com/sc-29410.pdf ) [ , ].

Techniques: