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m paratuberculosis 43525  (ATCC)


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    Structured Review

    ATCC m paratuberculosis 43525
    Chromosome features of <t> M. paratuberculosis 43525 </t>
    M Paratuberculosis 43525, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity"

    Article Title: Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity

    Journal: BMC Genomics

    doi: 10.1186/s12864-015-1889-2

    Chromosome features of  M. paratuberculosis 43525
    Figure Legend Snippet: Chromosome features of M. paratuberculosis 43525

    Techniques Used:

    Phylogenetic analysis of M. paratuberculosis 43525 relative to other M. paratuberculosis isolates and two M. avium isolates. Neighbour-joining phylogenetic tree based on SNPs of M. paratuberculosis isolates from bovine, ovine and human hosts and M. avium isolates derived from human and avian hosts with M. paratuberculosis K10 as the reference. The colour box indicates the clade in which M. paratuberculosis 43525 belongs and also contains human isolates M. paratuberculosis 4 and 4B. In addition, this clade contains recent (compared to the type strains) bovine isolates, including the bovine isolate CLIJ644. The reference strain M. paratuberculosis K10 is in a different clade to M. paratuberculosis 43525 and the type strain ATCC19698
    Figure Legend Snippet: Phylogenetic analysis of M. paratuberculosis 43525 relative to other M. paratuberculosis isolates and two M. avium isolates. Neighbour-joining phylogenetic tree based on SNPs of M. paratuberculosis isolates from bovine, ovine and human hosts and M. avium isolates derived from human and avian hosts with M. paratuberculosis K10 as the reference. The colour box indicates the clade in which M. paratuberculosis 43525 belongs and also contains human isolates M. paratuberculosis 4 and 4B. In addition, this clade contains recent (compared to the type strains) bovine isolates, including the bovine isolate CLIJ644. The reference strain M. paratuberculosis K10 is in a different clade to M. paratuberculosis 43525 and the type strain ATCC19698

    Techniques Used: Derivative Assay

    Comparison of the mycobactin cluster between members of the MAC complex and M. tuberculosis . Amino acid length is indicated above each gene, gene names are indicated and colour matched to equivalent genes in other strains ( M. paratuberculosis 43525, M. paratuberculosis K10, M. paratuberculosis S397 M. avium 104, M. avium ATCC25291 and M. tuberculosis ). M. paratuberculosis K10, M. paratuberculosis S397 and M. avium ATCC25291 are of animal origin, the remaining three are from human hosts. The NRPS modules MbtB and MbtE are one gene in M. paratuberculosis 43525 and M. tuberculosis but two genes in other MAC. In addition, all M. paratuberculosis strains had an mce operon ( mce 6) present in the gap between MbtA and MbtJ (indicated for M. paratuberculosis 43525 and K10)
    Figure Legend Snippet: Comparison of the mycobactin cluster between members of the MAC complex and M. tuberculosis . Amino acid length is indicated above each gene, gene names are indicated and colour matched to equivalent genes in other strains ( M. paratuberculosis 43525, M. paratuberculosis K10, M. paratuberculosis S397 M. avium 104, M. avium ATCC25291 and M. tuberculosis ). M. paratuberculosis K10, M. paratuberculosis S397 and M. avium ATCC25291 are of animal origin, the remaining three are from human hosts. The NRPS modules MbtB and MbtE are one gene in M. paratuberculosis 43525 and M. tuberculosis but two genes in other MAC. In addition, all M. paratuberculosis strains had an mce operon ( mce 6) present in the gap between MbtA and MbtJ (indicated for M. paratuberculosis 43525 and K10)

    Techniques Used:



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    Image Search Results


    Chromosome features of  M. paratuberculosis 43525

    Journal: BMC Genomics

    Article Title: Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity

    doi: 10.1186/s12864-015-1889-2

    Figure Lengend Snippet: Chromosome features of M. paratuberculosis 43525

    Article Snippet: The mce and mycobactin cluster genes were compared across MAC and M. tuberculosis with emphasis on members of the MAC complex that infect animals; M. paratuberculosis K10 (bovine), M. avium ATCC 25291 (avian), M. paratuberculosis S397 (sheep) and those that infect humans; M. paratuberculosis 43525, M. avium 104, M. avium TH135.

    Techniques:

    Phylogenetic analysis of M. paratuberculosis 43525 relative to other M. paratuberculosis isolates and two M. avium isolates. Neighbour-joining phylogenetic tree based on SNPs of M. paratuberculosis isolates from bovine, ovine and human hosts and M. avium isolates derived from human and avian hosts with M. paratuberculosis K10 as the reference. The colour box indicates the clade in which M. paratuberculosis 43525 belongs and also contains human isolates M. paratuberculosis 4 and 4B. In addition, this clade contains recent (compared to the type strains) bovine isolates, including the bovine isolate CLIJ644. The reference strain M. paratuberculosis K10 is in a different clade to M. paratuberculosis 43525 and the type strain ATCC19698

    Journal: BMC Genomics

    Article Title: Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity

    doi: 10.1186/s12864-015-1889-2

    Figure Lengend Snippet: Phylogenetic analysis of M. paratuberculosis 43525 relative to other M. paratuberculosis isolates and two M. avium isolates. Neighbour-joining phylogenetic tree based on SNPs of M. paratuberculosis isolates from bovine, ovine and human hosts and M. avium isolates derived from human and avian hosts with M. paratuberculosis K10 as the reference. The colour box indicates the clade in which M. paratuberculosis 43525 belongs and also contains human isolates M. paratuberculosis 4 and 4B. In addition, this clade contains recent (compared to the type strains) bovine isolates, including the bovine isolate CLIJ644. The reference strain M. paratuberculosis K10 is in a different clade to M. paratuberculosis 43525 and the type strain ATCC19698

    Article Snippet: The mce and mycobactin cluster genes were compared across MAC and M. tuberculosis with emphasis on members of the MAC complex that infect animals; M. paratuberculosis K10 (bovine), M. avium ATCC 25291 (avian), M. paratuberculosis S397 (sheep) and those that infect humans; M. paratuberculosis 43525, M. avium 104, M. avium TH135.

    Techniques: Derivative Assay

    Comparison of the mycobactin cluster between members of the MAC complex and M. tuberculosis . Amino acid length is indicated above each gene, gene names are indicated and colour matched to equivalent genes in other strains ( M. paratuberculosis 43525, M. paratuberculosis K10, M. paratuberculosis S397 M. avium 104, M. avium ATCC25291 and M. tuberculosis ). M. paratuberculosis K10, M. paratuberculosis S397 and M. avium ATCC25291 are of animal origin, the remaining three are from human hosts. The NRPS modules MbtB and MbtE are one gene in M. paratuberculosis 43525 and M. tuberculosis but two genes in other MAC. In addition, all M. paratuberculosis strains had an mce operon ( mce 6) present in the gap between MbtA and MbtJ (indicated for M. paratuberculosis 43525 and K10)

    Journal: BMC Genomics

    Article Title: Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity

    doi: 10.1186/s12864-015-1889-2

    Figure Lengend Snippet: Comparison of the mycobactin cluster between members of the MAC complex and M. tuberculosis . Amino acid length is indicated above each gene, gene names are indicated and colour matched to equivalent genes in other strains ( M. paratuberculosis 43525, M. paratuberculosis K10, M. paratuberculosis S397 M. avium 104, M. avium ATCC25291 and M. tuberculosis ). M. paratuberculosis K10, M. paratuberculosis S397 and M. avium ATCC25291 are of animal origin, the remaining three are from human hosts. The NRPS modules MbtB and MbtE are one gene in M. paratuberculosis 43525 and M. tuberculosis but two genes in other MAC. In addition, all M. paratuberculosis strains had an mce operon ( mce 6) present in the gap between MbtA and MbtJ (indicated for M. paratuberculosis 43525 and K10)

    Article Snippet: The mce and mycobactin cluster genes were compared across MAC and M. tuberculosis with emphasis on members of the MAC complex that infect animals; M. paratuberculosis K10 (bovine), M. avium ATCC 25291 (avian), M. paratuberculosis S397 (sheep) and those that infect humans; M. paratuberculosis 43525, M. avium 104, M. avium TH135.

    Techniques:

    OLFM4 decreases β-catenin levels. ( a–k ) Western blots were performed in 293T cells ( a–i ), SW480 cells ( j ) and freshly isolated mouse colon crypts ( k ) after human OLFM4 treatment (1 μg/ml) ( a–j ) and mouse Olfm4 treatment (1 μg/ml) ( k ). β-Actin, total cadherin, Hsp90 and SP1 were used as loading controls in total cell lysate, membrane, cytoplasm and nucleus cell preparations, respectively. ( a ) β-Catenin levels in total cell lysate, membrane, cytoplasm and nucleus at different time points of OLFM4 treatment. ( b ) Endogenous and Wnt3a (1 μg/ml)-induced β-catenin levels in the cytoplasm and nucleus of cells pretreated with and without OLFM4 for 30 min. ( c ) Endogenous and Wnt3a (1 μg/ml)-induced β-catenin levels in the cytoplasm of cells transfected with control (vector) or OLFM4 expression plasmid. ( d ) β-Catenin phosphorylation at different time points of OLFM4 treatment. ( e and f ) Akt (T308 or Ser473) phosphorylation at different time points of OLFM4 treatment. ( g and h ) β-Catenin levels in the cytoplasm of cells pretreated with and without either LiCl (20 m m ) or MG132 (10 μ m ) before OLFM4 treatment for 30 min. ( i ) β-Catenin levels in total cell lysates of cells transfected with Flag-tagged WT or mutant (S33Y or S31/37T41S45) β-catenin expression plasmids and then treated with or without OLFM4 for 30 min. ( j ) Total and active β-catenin levels in the cytoplasm or nucleus of SW480 colon-cancer cells at different time points of Olfm4 treatment. ( k ) β-Catenin levels in the cytoplasm and nucleus of freshly isolated mouse colon crypts at different time points of Olfm4 (or PBS control buffer) treatment. ( l ) Olfm4 and β-catenin levels in the cytoplasm of Ls174T cells infected with lentiviral control shRNA and Olfm4 shRNA.

    Journal: Oncogene

    Article Title: Olfactomedin 4 deletion induces colon adenocarcinoma in Apc Min/+ mice

    doi: 10.1038/onc.2016.58

    Figure Lengend Snippet: OLFM4 decreases β-catenin levels. ( a–k ) Western blots were performed in 293T cells ( a–i ), SW480 cells ( j ) and freshly isolated mouse colon crypts ( k ) after human OLFM4 treatment (1 μg/ml) ( a–j ) and mouse Olfm4 treatment (1 μg/ml) ( k ). β-Actin, total cadherin, Hsp90 and SP1 were used as loading controls in total cell lysate, membrane, cytoplasm and nucleus cell preparations, respectively. ( a ) β-Catenin levels in total cell lysate, membrane, cytoplasm and nucleus at different time points of OLFM4 treatment. ( b ) Endogenous and Wnt3a (1 μg/ml)-induced β-catenin levels in the cytoplasm and nucleus of cells pretreated with and without OLFM4 for 30 min. ( c ) Endogenous and Wnt3a (1 μg/ml)-induced β-catenin levels in the cytoplasm of cells transfected with control (vector) or OLFM4 expression plasmid. ( d ) β-Catenin phosphorylation at different time points of OLFM4 treatment. ( e and f ) Akt (T308 or Ser473) phosphorylation at different time points of OLFM4 treatment. ( g and h ) β-Catenin levels in the cytoplasm of cells pretreated with and without either LiCl (20 m m ) or MG132 (10 μ m ) before OLFM4 treatment for 30 min. ( i ) β-Catenin levels in total cell lysates of cells transfected with Flag-tagged WT or mutant (S33Y or S31/37T41S45) β-catenin expression plasmids and then treated with or without OLFM4 for 30 min. ( j ) Total and active β-catenin levels in the cytoplasm or nucleus of SW480 colon-cancer cells at different time points of Olfm4 treatment. ( k ) β-Catenin levels in the cytoplasm and nucleus of freshly isolated mouse colon crypts at different time points of Olfm4 (or PBS control buffer) treatment. ( l ) Olfm4 and β-catenin levels in the cytoplasm of Ls174T cells infected with lentiviral control shRNA and Olfm4 shRNA.

    Article Snippet: Lentiviral shRNA against TCF4, Olfm4 and control shRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and transduced into cells according to the manufacturer's instructions.

    Techniques: Western Blot, Isolation, Transfection, Plasmid Preparation, Expressing, Mutagenesis, Infection, shRNA

    OLFM4 is a target gene of the Wnt/β-catenin/TCF pathway. ( a ) Three LEF1/TCF binding sites (in black box) located in the promoter of OLFM4 . ( b ) The core (CAAAG or CTTTG) of the LEF1/TCF binding site was deleted individually or in combination as indicated by gray boxes. Data represent mean (±s.d.) ( n =3) relative luciferase activity of corresponding reporter in the presence of Wnt3a or vehicle in 293T cells. * P< 0.05 and ** P< 0.01 when compared with WT vehicle or WT Wnt3a, respectively, two-tailed t -test. ( c ) Mean (±s.d.) ( n =3) OLFM4 mRNA expression determined by qRT–PCR in Ls174T cells (right) after knockdown of TCF4 expression by TCF4 lentivirus shRNA (left). * P< 0.01 when compared with control (Con) shRNA, two-tailed t -test. ( d ) Mean (±s.d.) ( n =3) OLFM4 mRNA expression determined by qRT–PCR in Ls174T and SW948 cells treated with IWP2, XAV-939 or DMSO control. * P< 0.01 when compared with DMSO group, two-tailed t -test. ( e ) ChIP assay of LEF1 and TCF4 binding to all three LEF/TCF binding sites in the OLFM4 promoter.

    Journal: Oncogene

    Article Title: Olfactomedin 4 deletion induces colon adenocarcinoma in Apc Min/+ mice

    doi: 10.1038/onc.2016.58

    Figure Lengend Snippet: OLFM4 is a target gene of the Wnt/β-catenin/TCF pathway. ( a ) Three LEF1/TCF binding sites (in black box) located in the promoter of OLFM4 . ( b ) The core (CAAAG or CTTTG) of the LEF1/TCF binding site was deleted individually or in combination as indicated by gray boxes. Data represent mean (±s.d.) ( n =3) relative luciferase activity of corresponding reporter in the presence of Wnt3a or vehicle in 293T cells. * P< 0.05 and ** P< 0.01 when compared with WT vehicle or WT Wnt3a, respectively, two-tailed t -test. ( c ) Mean (±s.d.) ( n =3) OLFM4 mRNA expression determined by qRT–PCR in Ls174T cells (right) after knockdown of TCF4 expression by TCF4 lentivirus shRNA (left). * P< 0.01 when compared with control (Con) shRNA, two-tailed t -test. ( d ) Mean (±s.d.) ( n =3) OLFM4 mRNA expression determined by qRT–PCR in Ls174T and SW948 cells treated with IWP2, XAV-939 or DMSO control. * P< 0.01 when compared with DMSO group, two-tailed t -test. ( e ) ChIP assay of LEF1 and TCF4 binding to all three LEF/TCF binding sites in the OLFM4 promoter.

    Article Snippet: Lentiviral shRNA against TCF4, Olfm4 and control shRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and transduced into cells according to the manufacturer's instructions.

    Techniques: Binding Assay, Luciferase, Activity Assay, Two Tailed Test, Expressing, Quantitative RT-PCR, shRNA