Journal: Emerging Infectious Diseases

Article Title: Isolation of Novel Mycobacterium Species from Skin Infection in an Immunocompromised Person

doi: 10.3201/eid2711.210426

Figure Lengend Snippet: Novel Mycobacterium gordonae –like infection in a 63-year-old man in China. A) Verrucous dull nodule on the left shin of the patient. B) Mycobacterium colonies grown on Löwenstein–Jensen medium.

Article Snippet: Evaluating isDDH using the Type Strain Genome Server ( https://tygs.dsmz.de ) showed weak relations with M. gordonae DSM 44160 (34.5%) and M. paragordonae 49061 (31.3%) ( ) (Appendix Table); we found no closely related genome in the database.

Techniques: Infection

Journal: Emerging Infectious Diseases

Article Title: Isolation of Novel Mycobacterium Species from Skin Infection in an Immunocompromised Person

doi: 10.3201/eid2711.210426

Figure Lengend Snippet: Phylogenetic trees of isolate from novel Mycobacterium gordonae –like infection in a 63-year-old man in China (X7091) and reference isolates. A) Evolutionary tree involving 16S rRNA gene (1,067 positions) of isolate X7091 and 26 Mycobacterium strains. Tree constructed using the maximum-likelihood method and Tamura-Nei model with 500 bootstrap replications in MEGA X ( https://www.megasoftware.net ). We selected Norcadia seriolae ATCC 43993 as the outgroup. B) Core genome–based maximum-likelihood phylogeny of isolate X7091 and other M. gordonae –like strains analyzed by Roary ( https://sanger-pathogens.github.io/Roary ) and constructed with a general time-reversible plus gamma maximum model (500 bootstrap replications) using the RaxML tool . We selected Mycobacterium marinum ATCC 927 as the outgroup. Scale bars indicate the number of nucleotide substitutions per site.

Article Snippet: Evaluating isDDH using the Type Strain Genome Server ( https://tygs.dsmz.de ) showed weak relations with M. gordonae DSM 44160 (34.5%) and M. paragordonae 49061 (31.3%) ( ) (Appendix Table); we found no closely related genome in the database.

Techniques: Infection, Construct