Review

g thermoleovorans (ATCC)

Name: g thermoleovorans
Brand: ATCC
Rating: 92 (4 reviews)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC g thermoleovorans
Characterized and semi-characterized bacteriocins from the Geobacillus and Parageobacillus species.

G Thermoleovorans, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/g thermoleovorans/product/ATCC
Average 92 stars, based on 1 article reviews

g thermoleovorans - by Bioz Stars, 2025-04

92/100 stars

Images

1) Product Images from "Antimicrobial Potential of the Genera Geobacillus and Parageobacillus , as Well as Endolysins Biosynthesized by Their Bacteriophages"

Article Title: Antimicrobial Potential of the Genera Geobacillus and Parageobacillus , as Well as Endolysins Biosynthesized by Their Bacteriophages

Journal: Antibiotics

doi: 10.3390/antibiotics11020242

Figure Legend Snippet: Characterized and semi-characterized bacteriocins from the Geobacillus and Parageobacillus species.

Techniques Used: Activity Assay, Molecular Weight, Binding Assay, Incubation

Image Search Results

Journal: Antibiotics

Article Title: Antimicrobial Potential of the Genera Geobacillus and Parageobacillus , as Well as Endolysins Biosynthesized by Their Bacteriophages

doi: 10.3390/antibiotics11020242

Figure Lengend Snippet: Characterized and semi-characterized bacteriocins from the Geobacillus and Parageobacillus species.

Article Snippet: For example, it was demonstrated that G. thermoleovorans could inhibit the growth of some reference pathogenic strains, including S. typhimurium (ATCC1408) , Vibrio parahaemolytiticus (ATCC 17802), Vibrio alginolyticus (ATCC 17749) , S. aureus (ATCC 25923) and a β-lactamase-producing E. coli strain (ATCC 35218) [ , ].

Techniques: Activity Assay, Molecular Weight, Binding Assay, Incubation

In vitro activity of 10-DEBC. ( A ) The activity of 10-DEBC against M. abscessus in MH broth medium. DRC was plotted from the REMA. RFU, relative fluorescence units. ( B ) The activity of 10-DEBC against M. abscessus -Lux, incubated under the same condition as in panel B RLU, relative luciferase units. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Journal: International Journal of Molecular Sciences

Article Title: 10-DEBC Hydrochloride as a Promising New Agent against Infection of Mycobacterium abscessus

doi: 10.3390/ijms23020591

Figure Lengend Snippet: In vitro activity of 10-DEBC. ( A ) The activity of 10-DEBC against M. abscessus in MH broth medium. DRC was plotted from the REMA. RFU, relative fluorescence units. ( B ) The activity of 10-DEBC against M. abscessus -Lux, incubated under the same condition as in panel B RLU, relative luciferase units. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Article Snippet: The M. abscessus strain (DSMZ 44196) was grown at 37 °C in Middlebrook 7H9 broth (BD, 27130) supplemented with 10% OADC (BD, 212240).

Techniques: In Vitro, Activity Assay, Fluorescence, Incubation, Luciferase, Concentration Assay

The activity of 10-DEBC and antimicrobial agents against M. abscessus clinical isolates.

Journal: International Journal of Molecular Sciences

Article Title: 10-DEBC Hydrochloride as a Promising New Agent against Infection of Mycobacterium abscessus

doi: 10.3390/ijms23020591

Figure Lengend Snippet: The activity of 10-DEBC and antimicrobial agents against M. abscessus clinical isolates.

Article Snippet: The M. abscessus strain (DSMZ 44196) was grown at 37 °C in Middlebrook 7H9 broth (BD, 27130) supplemented with 10% OADC (BD, 212240).

Techniques: Activity Assay

The activity of 10-DEBC against clarithromycin-resistant M. abscessus mutants. Clarithromycin-resistant M. abscessus 100 μg/mL to 97 μg/mL of CLR and 10-DEBC. DRC of M. abscessus CLR-R mutant ( Left panel ). Resazurin reports bacterial viability via a color change from blue to pink ( Right panel ). Upon oxidation by live M. abscessus , it turns pink, indicating growth of M. abscessus . Each experiment was performed in triplicate. This result was made from a representative experiment. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Journal: International Journal of Molecular Sciences

Article Title: 10-DEBC Hydrochloride as a Promising New Agent against Infection of Mycobacterium abscessus

doi: 10.3390/ijms23020591

Figure Lengend Snippet: The activity of 10-DEBC against clarithromycin-resistant M. abscessus mutants. Clarithromycin-resistant M. abscessus 100 μg/mL to 97 μg/mL of CLR and 10-DEBC. DRC of M. abscessus CLR-R mutant ( Left panel ). Resazurin reports bacterial viability via a color change from blue to pink ( Right panel ). Upon oxidation by live M. abscessus , it turns pink, indicating growth of M. abscessus . Each experiment was performed in triplicate. This result was made from a representative experiment. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Article Snippet: The M. abscessus strain (DSMZ 44196) was grown at 37 °C in Middlebrook 7H9 broth (BD, 27130) supplemented with 10% OADC (BD, 212240).

Techniques: Activity Assay, Mutagenesis, Concentration Assay

DRC of 10-DEBC. DRC of M. abscessus under aerobic, anaerobic using REMA. M. abscessus was undergone for seven days within an anaerobic generating container system (BD GasPak™ EZ). The activity of CLR ( A ) and 10-DEBC ( B ) against M. abscessus under aerobic and anaerobic conditions. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Journal: International Journal of Molecular Sciences

Article Title: 10-DEBC Hydrochloride as a Promising New Agent against Infection of Mycobacterium abscessus

doi: 10.3390/ijms23020591

Figure Lengend Snippet: DRC of 10-DEBC. DRC of M. abscessus under aerobic, anaerobic using REMA. M. abscessus was undergone for seven days within an anaerobic generating container system (BD GasPak™ EZ). The activity of CLR ( A ) and 10-DEBC ( B ) against M. abscessus under aerobic and anaerobic conditions. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Article Snippet: The M. abscessus strain (DSMZ 44196) was grown at 37 °C in Middlebrook 7H9 broth (BD, 27130) supplemented with 10% OADC (BD, 212240).

Techniques: Activity Assay, Concentration Assay

MBEC biofilm antimicrobial viability assay of 10-DEBC. The MBEC biofilm viability assay can confirm the effects of 10-DEBC-induced eradication on M. abscessus within biofilms by measuring the metabolic activities of live M. abscessus within biofilms using the resazurin. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Journal: International Journal of Molecular Sciences

Article Title: 10-DEBC Hydrochloride as a Promising New Agent against Infection of Mycobacterium abscessus

doi: 10.3390/ijms23020591

Figure Lengend Snippet: MBEC biofilm antimicrobial viability assay of 10-DEBC. The MBEC biofilm viability assay can confirm the effects of 10-DEBC-induced eradication on M. abscessus within biofilms by measuring the metabolic activities of live M. abscessus within biofilms using the resazurin. The experiments were carried out with three biological replicates and expressed as the mean ± SEM for each concentration.

Article Snippet: The M. abscessus strain (DSMZ 44196) was grown at 37 °C in Middlebrook 7H9 broth (BD, 27130) supplemented with 10% OADC (BD, 212240).

Techniques: Viability Assay, Concentration Assay

10-DEBC promotes the killing of intracellular M. abscessus . ( A ) Dual reading assay of 10-DEBC within macrophages. Infections in macrophages were carried out with multiplicities of infection (MOI) of 2 bacteria per cell for three hours and washed to remove extracellular mycobacteria. Luminescence detected from luciferase-expressing M. abscessus alone within THP-1 macrophages. The infected THP-1 cells stained with SYTO 60 were counted under fluorescence detection, and total cells were counted using Multi Reader. ( B ) THP-1 activated with PMA were infected at MOI of 2 with GFP-expressed M. abscessus for three hours, followed by treatment with 10-DEBC indicated concentrations in fresh medium. Ratio sum intensity values were used for all data reduction steps. ( C ) iMACs were infected at MOI of 2 with GFP-expressed M. abscessus for three hours, followed by treatment with 10-DEBC indicated concentrations in fresh medium. ( D ) Images of GFP-expressing M. abscessus infected iMACs on day three after treatment with indicated concentrations of 10-DEBC. Automated microscopy using a Lionheart TM automated live-cell imager. The Gen5 TM 3.05 software object feature enables the identification of cells within the imaging field. Data were expressed as the mean ± SD of triplication for each concentration. (Scale bar = 100 μm.).

Journal: International Journal of Molecular Sciences

Article Title: 10-DEBC Hydrochloride as a Promising New Agent against Infection of Mycobacterium abscessus

doi: 10.3390/ijms23020591

Figure Lengend Snippet: 10-DEBC promotes the killing of intracellular M. abscessus . ( A ) Dual reading assay of 10-DEBC within macrophages. Infections in macrophages were carried out with multiplicities of infection (MOI) of 2 bacteria per cell for three hours and washed to remove extracellular mycobacteria. Luminescence detected from luciferase-expressing M. abscessus alone within THP-1 macrophages. The infected THP-1 cells stained with SYTO 60 were counted under fluorescence detection, and total cells were counted using Multi Reader. ( B ) THP-1 activated with PMA were infected at MOI of 2 with GFP-expressed M. abscessus for three hours, followed by treatment with 10-DEBC indicated concentrations in fresh medium. Ratio sum intensity values were used for all data reduction steps. ( C ) iMACs were infected at MOI of 2 with GFP-expressed M. abscessus for three hours, followed by treatment with 10-DEBC indicated concentrations in fresh medium. ( D ) Images of GFP-expressing M. abscessus infected iMACs on day three after treatment with indicated concentrations of 10-DEBC. Automated microscopy using a Lionheart TM automated live-cell imager. The Gen5 TM 3.05 software object feature enables the identification of cells within the imaging field. Data were expressed as the mean ± SD of triplication for each concentration. (Scale bar = 100 μm.).

Article Snippet: The M. abscessus strain (DSMZ 44196) was grown at 37 °C in Middlebrook 7H9 broth (BD, 27130) supplemented with 10% OADC (BD, 212240).

Techniques: Infection, Luciferase, Expressing, Staining, Fluorescence, Microscopy, Software, Imaging, Concentration Assay

Bacteria and reference strains used in this study

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation of Mycobacterium Species: A Pilot Study

doi:

Figure Lengend Snippet: Bacteria and reference strains used in this study

Article Snippet: M. abscessus , DSMZ 44196T.

Techniques:

GenBank sequence identification numbers (accession number) for Mycobacterium species

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation of Mycobacterium Species: A Pilot Study

doi:

Figure Lengend Snippet: GenBank sequence identification numbers (accession number) for Mycobacterium species

Article Snippet: M. abscessus , DSMZ 44196T.

Techniques: Sequencing

Sequences and the parameters of oligonucleotide probes targeting the Mycobacterium ITS. *

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation of Mycobacterium Species: A Pilot Study

doi:

Figure Lengend Snippet: Sequences and the parameters of oligonucleotide probes targeting the Mycobacterium ITS. *

Article Snippet: M. abscessus , DSMZ 44196T.

Techniques: Sequencing

Agarose gel electrophoresis of ITS-PCR products for standard Mycobacterium species. Lane 1: M. tuberculosis H37Rv, Lane 2: M. simiae (clinical isolate), Lane 3: M. intracellulare ATCC 13950, Lane 4: M. avium ATCC 25291, Lane 5: M. kansasii TMC 1204, Lane 6: M. chelonae (environmental isolate), Lane 7: M. abscessus DSMZ 44196T, Lane 8: M. fortuitum TMC 1530, Lane 9: M. scrofulaceum ATCC 35785, Lane10: M. ulcerans ATCC 35840, Lane 11: M. marinum (environmental isolate), Lane 12: M. gordonae (clinical isolate). Lane M: 100 bp DNA marker; Lane C-: negative control.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation of Mycobacterium Species: A Pilot Study

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of ITS-PCR products for standard Mycobacterium species. Lane 1: M. tuberculosis H37Rv, Lane 2: M. simiae (clinical isolate), Lane 3: M. intracellulare ATCC 13950, Lane 4: M. avium ATCC 25291, Lane 5: M. kansasii TMC 1204, Lane 6: M. chelonae (environmental isolate), Lane 7: M. abscessus DSMZ 44196T, Lane 8: M. fortuitum TMC 1530, Lane 9: M. scrofulaceum ATCC 35785, Lane10: M. ulcerans ATCC 35840, Lane 11: M. marinum (environmental isolate), Lane 12: M. gordonae (clinical isolate). Lane M: 100 bp DNA marker; Lane C-: negative control.

Article Snippet: M. abscessus , DSMZ 44196T.

Techniques: Agarose Gel Electrophoresis, Marker, Negative Control

LPAs for standard Mycobacterium species. Strip 1: M. tuberculosis H37Rv, strip 2: M. simiae (clinical isolate), strip 3: M. intracellulare ATCC 13950, strip 4: M. avium ATCC 25291, strip 5: M. kansasii TMC 1204, strip 6: M. chelonae (environmental isolate), strip 7: M. abscessus DSMZ 44196T, strip 8: M. fortuitum TMC 1530, strip 9: M. scrofulaceum ATCC 35785, strip 10: M. ulcerans ATCC 35840 and M. marinum (environmental isolate), strip 11: M. gordonae (clinical isolate). Briefly, species-specific probes in TE buffer were immobilized onto a nitrocellulose membrane (pore size 0.45 μm; Amersham Pharmacia Biotech) as 16 parallel lines. The membrane was cut into strips and biotinylated PCR products were denatured, added to the membranes, and incubated at 59 ºC for 30 min. The strips were then incubated with a 1:1000-diluted alkaline phosphatase-labeled streptavidin in TBS at pH 8 on a rotating platform at room temperature for 30 min. The strips were washed and BCIP/NBT was added and incubated with shaking at room temperature for 10 min in the dark. The color reactions occurred and the results were visually interpreted with a probe alignment guide, which shows the position of the probes in each line.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation of Mycobacterium Species: A Pilot Study

doi:

Figure Lengend Snippet: LPAs for standard Mycobacterium species. Strip 1: M. tuberculosis H37Rv, strip 2: M. simiae (clinical isolate), strip 3: M. intracellulare ATCC 13950, strip 4: M. avium ATCC 25291, strip 5: M. kansasii TMC 1204, strip 6: M. chelonae (environmental isolate), strip 7: M. abscessus DSMZ 44196T, strip 8: M. fortuitum TMC 1530, strip 9: M. scrofulaceum ATCC 35785, strip 10: M. ulcerans ATCC 35840 and M. marinum (environmental isolate), strip 11: M. gordonae (clinical isolate). Briefly, species-specific probes in TE buffer were immobilized onto a nitrocellulose membrane (pore size 0.45 μm; Amersham Pharmacia Biotech) as 16 parallel lines. The membrane was cut into strips and biotinylated PCR products were denatured, added to the membranes, and incubated at 59 ºC for 30 min. The strips were then incubated with a 1:1000-diluted alkaline phosphatase-labeled streptavidin in TBS at pH 8 on a rotating platform at room temperature for 30 min. The strips were washed and BCIP/NBT was added and incubated with shaking at room temperature for 10 min in the dark. The color reactions occurred and the results were visually interpreted with a probe alignment guide, which shows the position of the probes in each line.

Article Snippet: M. abscessus , DSMZ 44196T.

Techniques: Stripping Membranes, Incubation, Labeling

AHLs (A), AQs (B), and virulence factor production (C) in cocultures of P. aeruginosa PAO1 with M. abscessus subsp. abscessus DSM44196 or with M. abscessus subsp. massiliense P4a, normalized to solo cultures of P. aeruginosa PAO1. CFU counts after 8 h were 2.87 × 108 ± 1.91 × 107 CFU/ml for PAO1 in solo culture; 2.26 × 108 ± 1.46 × 107 and 2.11 × 107 ± 5.85 × 106 CFU/ml for PAO1 and M. abscessus DSM44196, respectively, in coculture; and 2.27 × 108 ± 1.20 × 107 and 4.9 × 107 ± 4.73 × 106 CFU/ml for PAO1 and M. abscessus subsp. massiliense P4a, respectively, in coculture. CFU counts after 24 h were 7.22 × 108 ± 4.01 × 107 CFU/ml for PAO1 in solo culture; 8.61 × 108 ± 1.12 × 107 and 3.68 × 104 ± 6.23 × 103 CFU/ml for PAO1 and M. abscessus DSM44196, respectively, in coculture; and 6.67 × 108 ± 5.09 × 107 and 5.08 × 105 ± 6.43 × 106 CFU/ml for PAO1 and M. abscessus subsp. massiliense P4a, respectively, in coculture. Data represent means ± standard errors (SE) from three independent biological replicates. Statistical analysis was carried out using analysis of variance (ANOVA) or a Tukey honestly significant difference (HSD) test. P values assigned to individual bars refer to solo cultures of P. aeruginosa PAO1 (*, P < 0.05; **, P < 0.01).

Journal: Infection and Immunity

Article Title: Interference with Pseudomonas aeruginosa Quorum Sensing and Virulence by the Mycobacterial Pseudomonas Quinolone Signal Dioxygenase AqdC in Combination with the N -Acylhomoserine Lactone Lactonase QsdA

doi: 10.1128/IAI.00278-19

Figure Lengend Snippet: AHLs (A), AQs (B), and virulence factor production (C) in cocultures of P. aeruginosa PAO1 with M. abscessus subsp. abscessus DSM44196 or with M. abscessus subsp. massiliense P4a, normalized to solo cultures of P. aeruginosa PAO1. CFU counts after 8 h were 2.87 × 108 ± 1.91 × 107 CFU/ml for PAO1 in solo culture; 2.26 × 108 ± 1.46 × 107 and 2.11 × 107 ± 5.85 × 106 CFU/ml for PAO1 and M. abscessus DSM44196, respectively, in coculture; and 2.27 × 108 ± 1.20 × 107 and 4.9 × 107 ± 4.73 × 106 CFU/ml for PAO1 and M. abscessus subsp. massiliense P4a, respectively, in coculture. CFU counts after 24 h were 7.22 × 108 ± 4.01 × 107 CFU/ml for PAO1 in solo culture; 8.61 × 108 ± 1.12 × 107 and 3.68 × 104 ± 6.23 × 103 CFU/ml for PAO1 and M. abscessus DSM44196, respectively, in coculture; and 6.67 × 108 ± 5.09 × 107 and 5.08 × 105 ± 6.43 × 106 CFU/ml for PAO1 and M. abscessus subsp. massiliense P4a, respectively, in coculture. Data represent means ± standard errors (SE) from three independent biological replicates. Statistical analysis was carried out using analysis of variance (ANOVA) or a Tukey honestly significant difference (HSD) test. P values assigned to individual bars refer to solo cultures of P. aeruginosa PAO1 (*, P < 0.05; **, P < 0.01).

Article Snippet: M. abscessus subsp. abscessus DSM44196 , Mycobacterial strain harboring aqd genes , DSMZ.

Techniques:

Journal: Infection and Immunity

doi: 10.1128/IAI.00278-19

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: M. abscessus subsp. abscessus DSM44196 , Mycobacterial strain harboring aqd genes , DSMZ.

Techniques: Plasmid Preparation, Expressing, Luciferase

Review

Structured Review

Images

1) Product Images from "Antimicrobial Potential of the Genera Geobacillus and Parageobacillus , as Well as Endolysins Biosynthesized by Their Bacteriophages"

Similar Products

Image Search Results