Journal: Stem Cells Translational Medicine
Article Title: Unraveling devitalization: its impact on immune response and ectopic bone remodeling from autologous and allogeneic callus mimics
doi: 10.1093/stcltm/szae063
Figure Lengend Snippet: Adaptive immune response to vital and devitalized CMs. (A) CD3+ cells represent T lymphocytes. (B) Quantification of CD3+ cells per field of view (FOV) at the periphery area surrounding the CMs and (C) CD3+ cells infiltrating the CMs at different time points. * P < .05. Noteworthy, due to the low retrievable sample number ( N = 2) for the vital syngeneic group, no statistical comparisons to this group could be made. (D) B220 + cells were primarily found in the bone marrow. Scale bars: 60 μm (black).
Article Snippet: For CD68 (ab31630, Abcam), CD206 (AF2535, R&D Systems), CD3 (ab16669, Abcam), iNOS (ab15323, Abcam), myeloperoxidase (MPO; CPTC-MPO-, DSHB), and B220/CD45R (NBP1-43408, Novus Biologicals) immunohistochemical staining to assess immunological responses, sections were incubated with 0.3% v/v H 2 O 2 for 15 minutes to block endogenous peroxidase activity, followed by 5% w/v BSA-phosphate buffered saline (BSA-PBS) for 30 minutes at room temperature to block nonspecific protein binding.
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