tenacibaculum maritimum  (ATCC)

Journal: Frontiers in Microbiology

Article Title: The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

doi: 10.3389/fmicb.2017.01542

Figure Lengend Snippet: Circular representation of the Tenacibaculum maritimum NCIMB 2154 T genome. Circles display (from the outside): (1) GC percent deviation (GC window – mean GC) in a 1,000-bp window. (2) Predicted CDSs transcribed in the clockwise direction. (3) Predicted CDSs transcribed in the counterclockwise direction. Genes displayed in (2) and (3) are color-coded according to the following categories: red and blue, MaGe validated annotations; purple, primary/automatic annotations. (4) GC skew (G + C/G – C) in a 1,000-bp window. (5) rRNA (blue), tRNA (green), miscellaneous other RNA (orange), transposable elements (pink), and pseudogenes (gray).

Article Snippet: Several batches of the T. maritimum type strain (i.e., NCIMB 2154 T , ATCC 43398 T , CIP 103528 T , and DSM 17995 T ), Tenacibaculum discolor LL04 11.1.1 T , Tenacibaculum jejuense CNURIC013 T , and Tenacibaculum soleae LL04 12.1.7 T were routinely grown in marine broth and agar 2216 (Difco) at 28°C and 170 rpm.

Techniques:

Journal: Frontiers in Microbiology

Article Title: The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

doi: 10.3389/fmicb.2017.01542

Figure Lengend Snippet: Tenacibaculum maritimum sphingomyelinase activity. Fluorescence measurement (arbitrary units) following incubation of 1.3 ng (blue line) of recombinant protein with Amplex Red Sphingomyelinase assay kit. The control conditions were as follows: negative control, 1.3 ng of boiled recombinant protein (green line); positive control, the purified sphingomyelinase from Bacillus cereus provided by the manufacturer (red line). Results correspond to the mean of triplicates and SDs are included.

Article Snippet: Several batches of the T. maritimum type strain (i.e., NCIMB 2154 T , ATCC 43398 T , CIP 103528 T , and DSM 17995 T ), Tenacibaculum discolor LL04 11.1.1 T , Tenacibaculum jejuense CNURIC013 T , and Tenacibaculum soleae LL04 12.1.7 T were routinely grown in marine broth and agar 2216 (Difco) at 28°C and 170 rpm.

Techniques: Activity Assay, Fluorescence, Incubation, Recombinant, Negative Control, Positive Control, Purification

Journal: Frontiers in Microbiology

Article Title: The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

doi: 10.3389/fmicb.2017.01542

Figure Lengend Snippet: Degradation of chondroitin sulfates by T. maritimum . (A) Colonies of T. maritimum NCIMB 2154 T , T. discolor LL04 11.1.1 T , T. jejuense KCTC 22618 T , and T. soleae LL04 12.1.7 T on marine agar 2216 supplemented with 0.2% of chondroitin sulfates A (left) and C (right). (B) Activity of the recombinant protein Tm CslA PL8 on chondroitin A (blue line) and chondroitin C (red line). The release of unsaturated oligosaccharides was monitored by spectrophotometry at 232 nm using biological triplicates. The two control conditions (green lines) correspond to the same reaction mixture with a boiled Tm CslA PL8 . For the clarity of the graphic, the error bars are only indicated every 10 s.

Article Snippet: Several batches of the T. maritimum type strain (i.e., NCIMB 2154 T , ATCC 43398 T , CIP 103528 T , and DSM 17995 T ), Tenacibaculum discolor LL04 11.1.1 T , Tenacibaculum jejuense CNURIC013 T , and Tenacibaculum soleae LL04 12.1.7 T were routinely grown in marine broth and agar 2216 (Difco) at 28°C and 170 rpm.

Techniques: Activity Assay, Recombinant, Spectrophotometry

Journal: Frontiers in Microbiology

Article Title: The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

doi: 10.3389/fmicb.2017.01542

Figure Lengend Snippet: Tenacibaculum maritimum sialidase activity. Fluorescence measurement (arbitrary units) following incubation of T. maritimum NCIMB 2154 T (red line), T. discolor LL04 11.1.1 T (blue line), T. jejuense KCTC 22618 T (green line), and T. soleae LL04 12.1.7 T (purple line) cells with the fluorogenic substrate MUAN. Results correspond to the mean of triplicates and SDs are shown.

Article Snippet: Several batches of the T. maritimum type strain (i.e., NCIMB 2154 T , ATCC 43398 T , CIP 103528 T , and DSM 17995 T ), Tenacibaculum discolor LL04 11.1.1 T , Tenacibaculum jejuense CNURIC013 T , and Tenacibaculum soleae LL04 12.1.7 T were routinely grown in marine broth and agar 2216 (Difco) at 28°C and 170 rpm.

Techniques: Activity Assay, Fluorescence, Incubation

Journal: Frontiers in Microbiology

Article Title: The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms

doi: 10.3389/fmicb.2017.01542

Figure Lengend Snippet: Summary of the predicted virulence-associated genes identified in this study in the genome of T. maritimum NCIMB 2154 T .

Article Snippet: Several batches of the T. maritimum type strain (i.e., NCIMB 2154 T , ATCC 43398 T , CIP 103528 T , and DSM 17995 T ), Tenacibaculum discolor LL04 11.1.1 T , Tenacibaculum jejuense CNURIC013 T , and Tenacibaculum soleae LL04 12.1.7 T were routinely grown in marine broth and agar 2216 (Difco) at 28°C and 170 rpm.

Techniques: Activity Assay