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p aeruginosa (ATCC)

Name: p aeruginosa
Brand: ATCC
Rating: 93 (16 reviews)

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ATCC p aeruginosa

Antimicrobial Efficacy of the ProGels against Common Wound Pathogens. A–C) These panels present the viability of common wound pathogens— P. <t>aeruginosa</t> , S. aureus , and C. albicans —after coincubating for 24 h with hydrogels, either loaded or unloaded with entrapped L. plantarum . The ProGel, with a high probiotic concentration (OD = 5), effectively eradicated both bacterial pathogens ( P. aeruginosa , S. aureus , A and B). Even the hydrogel with a lower probiotic load (OD = 0.5) managed to fully eliminate P. aeruginosa , while reducing S. aureus by roughly 3log CFU. C) The antifungal effect of the ProGels against C. albicans was not as strong as its antibacterial power, an elevation in the probiotic load within the hydrogel correlated with augmented antifungal activity, effectuating a reduction of 40% and 70% for a hydrogel with low (OD = 0.5) and high (OD = 5) probiotic loads, respectively. D–F) The agar diffusion assay demonstrates the hydrogel's antimicrobial properties against the mentioned pathogens. Notably, clear inhibition zones (indicated with the arrows and dotted circle) are visible against bacterial pathogens with the ProGels (D and E), especially those with higher probiotic loads. However, no significant inhibition zones were observed for C. albicans (F). *** denotes statistical difference ( P < 0.001) and ** ( P < 0.01) using the ANOVA test. n = 3 (biological repeats), mean ± SD shown.

P Aeruginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p aeruginosa/product/ATCC
Average 93 stars, based on 16 article reviews

p aeruginosa - by Bioz Stars, 2026-02

93/100 stars

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1) Product Images from "An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections"

Article Title: An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections

Journal: Advanced Healthcare Materials

doi: 10.1002/adhm.202400921

Antimicrobial Efficacy of the ProGels against Common Wound Pathogens. A–C) These panels present the viability of common wound pathogens— P. aeruginosa , S. aureus , and C. albicans —after coincubating for 24 h with hydrogels, either loaded or unloaded with entrapped L. plantarum . The ProGel, with a high probiotic concentration (OD = 5), effectively eradicated both bacterial pathogens ( P. aeruginosa , S. aureus , A and B). Even the hydrogel with a lower probiotic load (OD = 0.5) managed to fully eliminate P. aeruginosa , while reducing S. aureus by roughly 3log CFU. C) The antifungal effect of the ProGels against C. albicans was not as strong as its antibacterial power, an elevation in the probiotic load within the hydrogel correlated with augmented antifungal activity, effectuating a reduction of 40% and 70% for a hydrogel with low (OD = 0.5) and high (OD = 5) probiotic loads, respectively. D–F) The agar diffusion assay demonstrates the hydrogel's antimicrobial properties against the mentioned pathogens. Notably, clear inhibition zones (indicated with the arrows and dotted circle) are visible against bacterial pathogens with the ProGels (D and E), especially those with higher probiotic loads. However, no significant inhibition zones were observed for C. albicans (F). *** denotes statistical difference ( P < 0.001) and ** ( P < 0.01) using the ANOVA test. n = 3 (biological repeats), mean ± SD shown.

Figure Legend Snippet: Antimicrobial Efficacy of the ProGels against Common Wound Pathogens. A–C) These panels present the viability of common wound pathogens— P. aeruginosa , S. aureus , and C. albicans —after coincubating for 24 h with hydrogels, either loaded or unloaded with entrapped L. plantarum . The ProGel, with a high probiotic concentration (OD = 5), effectively eradicated both bacterial pathogens ( P. aeruginosa , S. aureus , A and B). Even the hydrogel with a lower probiotic load (OD = 0.5) managed to fully eliminate P. aeruginosa , while reducing S. aureus by roughly 3log CFU. C) The antifungal effect of the ProGels against C. albicans was not as strong as its antibacterial power, an elevation in the probiotic load within the hydrogel correlated with augmented antifungal activity, effectuating a reduction of 40% and 70% for a hydrogel with low (OD = 0.5) and high (OD = 5) probiotic loads, respectively. D–F) The agar diffusion assay demonstrates the hydrogel's antimicrobial properties against the mentioned pathogens. Notably, clear inhibition zones (indicated with the arrows and dotted circle) are visible against bacterial pathogens with the ProGels (D and E), especially those with higher probiotic loads. However, no significant inhibition zones were observed for C. albicans (F). *** denotes statistical difference ( P < 0.001) and ** ( P < 0.01) using the ANOVA test. n = 3 (biological repeats), mean ± SD shown.

Techniques Used: Concentration Assay, Activity Assay, Diffusion-based Assay, Inhibition

Cytotoxicity and hemocompatibility of ProGel. A) Hydrogel extracts (24 and 48 h) maintained high cell viability, indicating excellent cytocompatibility. B) nHDFs cocultured with L. plantarum showed no cytotoxicity, unlike those with P. aeruginosa and S. aureus , confirmed by the CCK‐8 assay. C) Microscopy shows detached, round dead cells in cultures with P. aeruginosa and S. aureus , while cells with L. plantarum remained intact. Scale bar = 100 µm. D) A transwell (0.4 µm pore size) allowed the separation of components, ensuring signals from nHDFs only. E) CCK‐8 assay results show no cytotoxicity from the living hydrogel after one day of coculture. F) Hemolysis assay results indicate all tested materials, except the Triton‐X control, had hemolysis rates below the 2% safety threshold, confirming hemocompatibility. *** denotes statistical significance ( P < 0.0001) using ANOVA test. n = 3 (biological repeats), mean ± SD shown.

Figure Legend Snippet: Cytotoxicity and hemocompatibility of ProGel. A) Hydrogel extracts (24 and 48 h) maintained high cell viability, indicating excellent cytocompatibility. B) nHDFs cocultured with L. plantarum showed no cytotoxicity, unlike those with P. aeruginosa and S. aureus , confirmed by the CCK‐8 assay. C) Microscopy shows detached, round dead cells in cultures with P. aeruginosa and S. aureus , while cells with L. plantarum remained intact. Scale bar = 100 µm. D) A transwell (0.4 µm pore size) allowed the separation of components, ensuring signals from nHDFs only. E) CCK‐8 assay results show no cytotoxicity from the living hydrogel after one day of coculture. F) Hemolysis assay results indicate all tested materials, except the Triton‐X control, had hemolysis rates below the 2% safety threshold, confirming hemocompatibility. *** denotes statistical significance ( P < 0.0001) using ANOVA test. n = 3 (biological repeats), mean ± SD shown.

Techniques Used: CCK-8 Assay, Microscopy, Pore Size, Hemolysis Assay, Control

A) Histological Analysis of the Antibiofilm Efficacy of Hydrogels Tested with an Ex vivo Skin Model. Artificial wounds of 6 mm diameter were created on human ex vivo skin samples of 13 mm diameter (A), followed by deliberate infection with P. aeruginosa (OD = 0.1) or S. aureus (OD = 0.5). Both unloaded and L. plantarum loaded hydrogel samples were applied on these infected wounds and subjected to treatment for 24 h ( P. aeruginosa ) and 48 h ( S. aureus ). Panels (B‐D) feature skin samples post‐ P. aeruginosa infection, B) untreated, C) treated with blank hydrogel, and D) L. plantarum loaded hydrogel. Panels (E–G) correspondingly display skin samples post‐ S. aureus infection, E) untreated, F) blank hydrogel treated, and G) L. plantarum loaded hydrogel treated. In the untreated groups, prominent clusters of B) P. aeruginosa and E) S. aureus biofilms were discernible on the dermis. Notably, while the blank hydrogel significantly F) curbed S. aureus biofilm formation, C) its effects against P. aeruginosa biofilms were inconsequential. With the ProGel treatment, the presence of biofilms was virtually absent (D, G), thereby reinforcing the potency of the living hydrogel against both pathogens. [Scale bar = 200 µm].

Figure Legend Snippet: A) Histological Analysis of the Antibiofilm Efficacy of Hydrogels Tested with an Ex vivo Skin Model. Artificial wounds of 6 mm diameter were created on human ex vivo skin samples of 13 mm diameter (A), followed by deliberate infection with P. aeruginosa (OD = 0.1) or S. aureus (OD = 0.5). Both unloaded and L. plantarum loaded hydrogel samples were applied on these infected wounds and subjected to treatment for 24 h ( P. aeruginosa ) and 48 h ( S. aureus ). Panels (B‐D) feature skin samples post‐ P. aeruginosa infection, B) untreated, C) treated with blank hydrogel, and D) L. plantarum loaded hydrogel. Panels (E–G) correspondingly display skin samples post‐ S. aureus infection, E) untreated, F) blank hydrogel treated, and G) L. plantarum loaded hydrogel treated. In the untreated groups, prominent clusters of B) P. aeruginosa and E) S. aureus biofilms were discernible on the dermis. Notably, while the blank hydrogel significantly F) curbed S. aureus biofilm formation, C) its effects against P. aeruginosa biofilms were inconsequential. With the ProGel treatment, the presence of biofilms was virtually absent (D, G), thereby reinforcing the potency of the living hydrogel against both pathogens. [Scale bar = 200 µm].

Techniques Used: Ex Vivo, Infection

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Journal: Advanced Healthcare Materials

Article Title: An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections

doi: 10.1002/adhm.202400921

Figure Lengend Snippet: Antimicrobial Efficacy of the ProGels against Common Wound Pathogens. A–C) These panels present the viability of common wound pathogens— P. aeruginosa , S. aureus , and C. albicans —after coincubating for 24 h with hydrogels, either loaded or unloaded with entrapped L. plantarum . The ProGel, with a high probiotic concentration (OD = 5), effectively eradicated both bacterial pathogens ( P. aeruginosa , S. aureus , A and B). Even the hydrogel with a lower probiotic load (OD = 0.5) managed to fully eliminate P. aeruginosa , while reducing S. aureus by roughly 3log CFU. C) The antifungal effect of the ProGels against C. albicans was not as strong as its antibacterial power, an elevation in the probiotic load within the hydrogel correlated with augmented antifungal activity, effectuating a reduction of 40% and 70% for a hydrogel with low (OD = 0.5) and high (OD = 5) probiotic loads, respectively. D–F) The agar diffusion assay demonstrates the hydrogel's antimicrobial properties against the mentioned pathogens. Notably, clear inhibition zones (indicated with the arrows and dotted circle) are visible against bacterial pathogens with the ProGels (D and E), especially those with higher probiotic loads. However, no significant inhibition zones were observed for C. albicans (F). *** denotes statistical difference ( P < 0.001) and ** ( P < 0.01) using the ANOVA test. n = 3 (biological repeats), mean ± SD shown.

Article Snippet: L. plantarum (ATCC 10 241), P. aeruginosa (ATCC 43 390), S. aureus (ATCC 6538), and C. albicans (ATCC 90 028) were purchased from the Leibniz Institute DSMZ.

Techniques: Concentration Assay, Activity Assay, Diffusion-based Assay, Inhibition

Journal: Advanced Healthcare Materials

Article Title: An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections

doi: 10.1002/adhm.202400921

Figure Lengend Snippet: Cytotoxicity and hemocompatibility of ProGel. A) Hydrogel extracts (24 and 48 h) maintained high cell viability, indicating excellent cytocompatibility. B) nHDFs cocultured with L. plantarum showed no cytotoxicity, unlike those with P. aeruginosa and S. aureus , confirmed by the CCK‐8 assay. C) Microscopy shows detached, round dead cells in cultures with P. aeruginosa and S. aureus , while cells with L. plantarum remained intact. Scale bar = 100 µm. D) A transwell (0.4 µm pore size) allowed the separation of components, ensuring signals from nHDFs only. E) CCK‐8 assay results show no cytotoxicity from the living hydrogel after one day of coculture. F) Hemolysis assay results indicate all tested materials, except the Triton‐X control, had hemolysis rates below the 2% safety threshold, confirming hemocompatibility. *** denotes statistical significance ( P < 0.0001) using ANOVA test. n = 3 (biological repeats), mean ± SD shown.

Article Snippet: L. plantarum (ATCC 10 241), P. aeruginosa (ATCC 43 390), S. aureus (ATCC 6538), and C. albicans (ATCC 90 028) were purchased from the Leibniz Institute DSMZ.

Techniques: CCK-8 Assay, Microscopy, Pore Size, Hemolysis Assay, Control

Journal: Advanced Healthcare Materials

Article Title: An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections

doi: 10.1002/adhm.202400921

Figure Lengend Snippet: A) Histological Analysis of the Antibiofilm Efficacy of Hydrogels Tested with an Ex vivo Skin Model. Artificial wounds of 6 mm diameter were created on human ex vivo skin samples of 13 mm diameter (A), followed by deliberate infection with P. aeruginosa (OD = 0.1) or S. aureus (OD = 0.5). Both unloaded and L. plantarum loaded hydrogel samples were applied on these infected wounds and subjected to treatment for 24 h ( P. aeruginosa ) and 48 h ( S. aureus ). Panels (B‐D) feature skin samples post‐ P. aeruginosa infection, B) untreated, C) treated with blank hydrogel, and D) L. plantarum loaded hydrogel. Panels (E–G) correspondingly display skin samples post‐ S. aureus infection, E) untreated, F) blank hydrogel treated, and G) L. plantarum loaded hydrogel treated. In the untreated groups, prominent clusters of B) P. aeruginosa and E) S. aureus biofilms were discernible on the dermis. Notably, while the blank hydrogel significantly F) curbed S. aureus biofilm formation, C) its effects against P. aeruginosa biofilms were inconsequential. With the ProGel treatment, the presence of biofilms was virtually absent (D, G), thereby reinforcing the potency of the living hydrogel against both pathogens. [Scale bar = 200 µm].

Article Snippet: L. plantarum (ATCC 10 241), P. aeruginosa (ATCC 43 390), S. aureus (ATCC 6538), and C. albicans (ATCC 90 028) were purchased from the Leibniz Institute DSMZ.

Techniques: Ex Vivo, Infection

Contribution of apoptosis and autophagy to PF-429242/chloroquine (CQ)-induced cell death. (A) PLC5 and HepG2 cells were treated for 48 h with 10 µM PF-429242, 20 µM CQ, their combination (10 µM PF-429242 + 20 µM CQ), or 0.5 µM doxorubicin (DX, for PLC5 cells). Protein expression levels were analyzed via Western blotting. (B) PLC5 and HepG2 cells were transfected with LC3B siRNA for 24 h, followed by treatment with 20 µM PF-429242 for an additional 24 h. Western blotting was used to assess protein expression levels. (C) LC3B siRNA-transfected PLC5 and HepG2 cells were replated in 96-well plates and treated with a combination of 10 µM PF-429242 and 20 µM CQ (PF/CQ) for 72 h. Cell viability was evaluated using alamarBlue staining.

Journal: International Journal of Medical Sciences

Article Title: The combination of PF-429242 and chloroquine triggers pH-dependent cell death in hepatocellular carcinoma cells

doi: 10.7150/ijms.109069

Figure Lengend Snippet: Contribution of apoptosis and autophagy to PF-429242/chloroquine (CQ)-induced cell death. (A) PLC5 and HepG2 cells were treated for 48 h with 10 µM PF-429242, 20 µM CQ, their combination (10 µM PF-429242 + 20 µM CQ), or 0.5 µM doxorubicin (DX, for PLC5 cells). Protein expression levels were analyzed via Western blotting. (B) PLC5 and HepG2 cells were transfected with LC3B siRNA for 24 h, followed by treatment with 20 µM PF-429242 for an additional 24 h. Western blotting was used to assess protein expression levels. (C) LC3B siRNA-transfected PLC5 and HepG2 cells were replated in 96-well plates and treated with a combination of 10 µM PF-429242 and 20 µM CQ (PF/CQ) for 72 h. Cell viability was evaluated using alamarBlue staining.

Article Snippet: After transfection for 24-48 h, cells were used for further experiments. siRNA specific for the LC3B (#sc-43390), ATP6V0D1 (#sc-63207), MBTPS1 (#sc-36496), and MBTPS2 (#sc-41652) genes were purchased from Santa Cruz Technology. pEGFP-STAT3 (#111934), pcDNA3-SREBP1a (#26801), pcDNA3-SREBP1c (#26802), and pcDNA3-SREBP2 (#26807) plasmids were purchased from Addgene.

Techniques: Expressing, Western Blot, Transfection, Staining

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1) Product Images from "An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections"

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