Journal: Frontiers in Microbiology

Article Title: Growth Coordination Between Butyrate-Oxidizing Syntrophs and Hydrogenotrophic Methanogens

doi: 10.3389/fmicb.2021.742531

Figure Lengend Snippet: The production of CH 4 and H 2 from butyrate oxidation (A) by a mesophilic coculture consisting of Syntrophomonas wolfei (S.w.) and Methanococcus maripaludis (M.m.) and their growth synchronization (B,C) . Three levels of syntroph-to-methanogen cell ratios (i.e., 5:1, 3:2, and 1:5) were created at the beginning by inoculating different population sizes to fresh medium, which were designated as the high, middle, and low cell ratio treatments, respectively. The relative growth (B) of the individual populations was estimated as the fold change of cell number at any timepoint relative to that at the beginning. Note that the change in Syntrophomonas – Methanococcus cell ratio over time (C) is shown in normal scale. The error bars in (A,B) indicate the positive values of standard deviation of four replicates.

Article Snippet: Syntrophomonas wolfei G311 (DSM102351), S. lipocalidus TGB-C1 (DSM12680), and M. maripaludis S2 (DSM14266) were purchased from German culture collection DSMZ (Braunschweig, Germany).

Techniques: Standard Deviation

Journal: Microorganisms

Article Title: How Capsular Exopolysaccharides Affect Cell Surface Properties of Lactic Acid Bacteria

doi: 10.3390/microorganisms8121904

Figure Lengend Snippet: Microscopic images of the cell suspensions of S. thermophilus ST143+ ( a , b ), ST143− ( c , d ), DGCC7984 ( e , f ), and W. cibaria DSM14295 ( g , h ) stained with India ink for CPS visualization, before (left) and after (right) ultrasonic treatment for CPS removal. Magnification: 1000×, scale bar: 10 µm. Arrows indicate microbial cell chains.

Article Snippet: S. thermophilus ST143+ and ST143− were provided by Christian Hansen A/S (Hørsholm, Denmark), S. thermophilus DGCC7984 by Danisco Deutschland GmbH (Niebüll, Germany), and W. cibaria DSM14295 by Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

Techniques: Staining

Journal: Microorganisms

Article Title: How Capsular Exopolysaccharides Affect Cell Surface Properties of Lactic Acid Bacteria

doi: 10.3390/microorganisms8121904

Figure Lengend Snippet: Comparison of the cells of S. thermophilus ST143+ ( a , b ), ST143− ( c , d ), and W. cibaria DSM14295 ( e , f ) before (left) and after (right) ultrasonic treatment for CPS removal as demonstrated by SEM images. Z: Z-ring; S: septum; scale bar: 500 nm.

Article Snippet: S. thermophilus ST143+ and ST143− were provided by Christian Hansen A/S (Hørsholm, Denmark), S. thermophilus DGCC7984 by Danisco Deutschland GmbH (Niebüll, Germany), and W. cibaria DSM14295 by Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

Techniques:

Journal: Microorganisms

Article Title: How Capsular Exopolysaccharides Affect Cell Surface Properties of Lactic Acid Bacteria

doi: 10.3390/microorganisms8121904

Figure Lengend Snippet: Cell surface hydrophobicity (CSH) of cells from S. thermophilus ST143+, ST143−, DGCC7984, and W. cibaria DSM14295 before (violet) and after ultrasonic treatment for CPS removal (orange). Mean values within the same strain with different superscripts differed significantly (α = 0.05).

Article Snippet: S. thermophilus ST143+ and ST143− were provided by Christian Hansen A/S (Hørsholm, Denmark), S. thermophilus DGCC7984 by Danisco Deutschland GmbH (Niebüll, Germany), and W. cibaria DSM14295 by Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

Techniques: Cell Surface Hydrophobicity

Journal: Scientific Reports

Article Title: Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants

doi: 10.1038/s41598-017-06743-6

Figure Lengend Snippet: Bacterial species used for specificity tests, P-values, probe specific (sSNR) and not specific (nsSNR) signal-to-noise ratios.

Article Snippet: Genomic DNA from Streptococcus agalactiae DSM2134, Streptococcus mutans DSM20523, Streptococcus oralis DSM20627, Gemella morbillorum DSM20572, Selenomonas noxia DSM19578, Veillonella parvula DSM2008, Parvimonas micra DSM20468, Actinomyces odontolyticus DSM19120, Propionibacterium acidifaciens DSM21887, Eikenella corrodens DSM8340, Campylobacter sputorum subsp. sputorum DSM10535, Haemophilus parainfluenzae DSM8978, Aggregatibacter actinomycetemcomitans DSM8324, Prevotella denticola DSM20614, Prevotella intermedia DSM20706, Prevotella melaninogenica DSM7089, Porphyromonas gingivalis DSM20709, Capnocytophaga sputigena DSM7273, Fusobacterium nucleatum subsp. polymorphum DSM20482, Leptotrichia buccalis DSM1135, and Treponema denticola DSM14222 was directly obtained from the DSMZ (Braunschweig, Germany).

Techniques:

Journal: Scientific Reports

Article Title: Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants

doi: 10.1038/s41598-017-06743-6

Figure Lengend Snippet: Sensitivity of the OralArray on DNA mixes. Artificial mixes of 16 S rRNA amplicons were subjected to LDR-UA analysis, sSNR values were plotted vs ng of each DNA amplicon. sSNR values were obtained with decreasing amounts of DNA mixes containing: ( a ) S. oralis, S. aureus, P. micra, P. acidifaciens, A. odontolyticus , and E. corrodens 16 S rRNA amplicons (8–1 ng each); ( b ) G. morbillorum, L. buccalis, F. nucleatum, T. denticola, C. sputigena , and P. gingivalis 16 S rRNA amplicons (8–1 ng each); ( c ) H. parainfluenzae, A. actinomycetemcomitans, C. sputorum, P. denticola, P. intermedia , and P. melaninogenica 16 S rRNA amplicons (8–1 ng each); ( d ) S. mutans, L. acidophilus, S. noxia , and V. parvula 16 S rRNA amplicons (8–1 ng each); ( e ) S. oralis, G. morbillorum, A. odontolyticus , and P. melaninogenica 16 S rRNA amplicons (8–1 ng each) added with human genomic DNA up to 100 ng of total DNA (68–96 ng).

Article Snippet: Genomic DNA from Streptococcus agalactiae DSM2134, Streptococcus mutans DSM20523, Streptococcus oralis DSM20627, Gemella morbillorum DSM20572, Selenomonas noxia DSM19578, Veillonella parvula DSM2008, Parvimonas micra DSM20468, Actinomyces odontolyticus DSM19120, Propionibacterium acidifaciens DSM21887, Eikenella corrodens DSM8340, Campylobacter sputorum subsp. sputorum DSM10535, Haemophilus parainfluenzae DSM8978, Aggregatibacter actinomycetemcomitans DSM8324, Prevotella denticola DSM20614, Prevotella intermedia DSM20706, Prevotella melaninogenica DSM7089, Porphyromonas gingivalis DSM20709, Capnocytophaga sputigena DSM7273, Fusobacterium nucleatum subsp. polymorphum DSM20482, Leptotrichia buccalis DSM1135, and Treponema denticola DSM14222 was directly obtained from the DSMZ (Braunschweig, Germany).

Techniques: Amplification