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Journal: PLoS ONE
Article Title: Impairment of IFN-Gamma Response to Synthetic Peptides of Mycobacterium tuberculosis in a 7-Day Whole Blood Assay
doi: 10.1371/journal.pone.0071351
Figure Lengend Snippet: The data indicates the presence of Calgranulin A after 7 days of culture, but not on Day 0.
Article Snippet: Protein gels were transferred to Hybond-C Extra nitrocellulose membranes (GE Healthcare, Pittsburgh, PA) in an XCell II blot electrophoretic cell (Life Technologies, Grand Island, NY) at 20 V for 60–90 min. Each membrane was blocked in PBS/3% BSA/0.1% Tween 20 for 1 hr at RT and probed with monoclonal anti-ESAT-6 (HYB 76-8CS), polyclonal anti-CFP-10 (K8493) antibodies (both from Statens Serum Institute, Copenhagen, Denmark), or
Techniques: