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mrpa  (ATCC)


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    Structured Review

    ATCC mrpa
    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
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    Images

    1) Product Images from "An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers"

    Article Title: An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2024.11.029

    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
    Figure Legend Snippet: Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

    Techniques Used: Zeta Potential Analyzer, Incubation, Staining



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    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
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    A. In FACS, two cell surface markers (EpCAM-Pecy7 and CD49f-APC) used to identify basal/stem cells (TICs, CD49f high EpCAM low , 57.6%) and luminal cells (NTCs, CD49f low EpCAM high , 34.1%) in primary breast tumor. B. Quantitative PCR showed that <t>Krt14</t> and p63 were highly expressed in basal/stem cells, while krt18 and E-cadherin (Ecad) were highly expressed in luminal cells. C. Quantitative PCR showed a higher expression of Yap1 and embryonic stem factors (Oct-4, Nanog, Sox2, and Klf4) in basal/stem cells than in luminal cells. D. RNA sequencing (RNA-seq) showed a higher expression of Yap1 mRNA in TICs than in NTCs. E. Western blotting showed a significantly higher expression of Yap1 protein in TICs than in NTCs. Nuclear (Nuc.) Yap1 (i.e., active YAP1) was only detected in TICs while NTCs only contain cytoplasmic (Cyto) Yap1. There was no increase of TAZ in TICs compared with NTCs. Histone 4 (H4) and Hsp90 proteins were used as internal controls to show similar loading of Nuc and Cyto proteins, respectively. F. Dual-color immunofluorescence staining with TIC marker (Krt14, green) and Yap1 (red) antibodies. Although cytoplasmic Yap1 was detected in all tumor cells, strong nuclear expression of Yap1 was detected specifically in Krt14-positive TICs.
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    Image Search Results


    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

    Journal: Bioactive Materials

    Article Title: An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers

    doi: 10.1016/j.bioactmat.2024.11.029

    Figure Lengend Snippet: Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

    Article Snippet: We selected MRSA (ATCC 43310) and MRPA (ATCC 27853) as test bacteria.

    Techniques: Zeta Potential Analyzer, Incubation, Staining

    A. In FACS, two cell surface markers (EpCAM-Pecy7 and CD49f-APC) used to identify basal/stem cells (TICs, CD49f high EpCAM low , 57.6%) and luminal cells (NTCs, CD49f low EpCAM high , 34.1%) in primary breast tumor. B. Quantitative PCR showed that Krt14 and p63 were highly expressed in basal/stem cells, while krt18 and E-cadherin (Ecad) were highly expressed in luminal cells. C. Quantitative PCR showed a higher expression of Yap1 and embryonic stem factors (Oct-4, Nanog, Sox2, and Klf4) in basal/stem cells than in luminal cells. D. RNA sequencing (RNA-seq) showed a higher expression of Yap1 mRNA in TICs than in NTCs. E. Western blotting showed a significantly higher expression of Yap1 protein in TICs than in NTCs. Nuclear (Nuc.) Yap1 (i.e., active YAP1) was only detected in TICs while NTCs only contain cytoplasmic (Cyto) Yap1. There was no increase of TAZ in TICs compared with NTCs. Histone 4 (H4) and Hsp90 proteins were used as internal controls to show similar loading of Nuc and Cyto proteins, respectively. F. Dual-color immunofluorescence staining with TIC marker (Krt14, green) and Yap1 (red) antibodies. Although cytoplasmic Yap1 was detected in all tumor cells, strong nuclear expression of Yap1 was detected specifically in Krt14-positive TICs.

    Journal: Oncotarget

    Article Title: Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling

    doi: 10.18632/oncotarget.6655

    Figure Lengend Snippet: A. In FACS, two cell surface markers (EpCAM-Pecy7 and CD49f-APC) used to identify basal/stem cells (TICs, CD49f high EpCAM low , 57.6%) and luminal cells (NTCs, CD49f low EpCAM high , 34.1%) in primary breast tumor. B. Quantitative PCR showed that Krt14 and p63 were highly expressed in basal/stem cells, while krt18 and E-cadherin (Ecad) were highly expressed in luminal cells. C. Quantitative PCR showed a higher expression of Yap1 and embryonic stem factors (Oct-4, Nanog, Sox2, and Klf4) in basal/stem cells than in luminal cells. D. RNA sequencing (RNA-seq) showed a higher expression of Yap1 mRNA in TICs than in NTCs. E. Western blotting showed a significantly higher expression of Yap1 protein in TICs than in NTCs. Nuclear (Nuc.) Yap1 (i.e., active YAP1) was only detected in TICs while NTCs only contain cytoplasmic (Cyto) Yap1. There was no increase of TAZ in TICs compared with NTCs. Histone 4 (H4) and Hsp90 proteins were used as internal controls to show similar loading of Nuc and Cyto proteins, respectively. F. Dual-color immunofluorescence staining with TIC marker (Krt14, green) and Yap1 (red) antibodies. Although cytoplasmic Yap1 was detected in all tumor cells, strong nuclear expression of Yap1 was detected specifically in Krt14-positive TICs.

    Article Snippet: Primary antibodies used in current study included anti-mouse Yap1 (Abcam, #ab56701), TAZ (Cell signaling, #4883), Histone H4 (Santa Cruz, #sc-25260), Hsp90 (Santa Cruz, #sc-8262), p-Smad2 (Santa Cruz, #sc-101801), Smad3 (Cell signaling, #9513), p-Smad3 (Santa Cruz, #sc-11769), Krt14 (Santa Cruz, #sc-43310), Krt18 (Santa Cruz, #sc-45406) and p63 (Abcam, #ab124762).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, RNA Sequencing Assay, Western Blot, Immunofluorescence, Staining, Marker

    A. After lentivirus transfection, western blotting showed a more active form of Yap1 in the nucleus in lentivirus-Yap1 transfected TICs than in parental TICs and empty vector (EV) transfected TICs. B. 1 st passage of breast TICs and NTCs harboring active Yap1 generated more colonies in 3-dimentional (3D) culture system. 3 rd passage of breast TICs harboring ectopic active Yap1 yielded more colonies. C. Colony number count of TICs and NTCs in the 1 st passage from B. (*, p < 0.001, n = 8). D. Colony number count of TICs and NTCs in the 2 nd and 3 rd passage from B. (*, p < 0.001, n = 6). E. Immunostaining analysis of colonies formed by TICs with or without ectopic expression of active Yap1. TIC markers (p63 and Krt14) and NTC markers (Krt18). Laser confocal microscopy. F. Image histogram analysis of images from E. . Percentage of red Krt14+ cells was indicated (*, p < 0.01, n = 15).

    Journal: Oncotarget

    Article Title: Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling

    doi: 10.18632/oncotarget.6655

    Figure Lengend Snippet: A. After lentivirus transfection, western blotting showed a more active form of Yap1 in the nucleus in lentivirus-Yap1 transfected TICs than in parental TICs and empty vector (EV) transfected TICs. B. 1 st passage of breast TICs and NTCs harboring active Yap1 generated more colonies in 3-dimentional (3D) culture system. 3 rd passage of breast TICs harboring ectopic active Yap1 yielded more colonies. C. Colony number count of TICs and NTCs in the 1 st passage from B. (*, p < 0.001, n = 8). D. Colony number count of TICs and NTCs in the 2 nd and 3 rd passage from B. (*, p < 0.001, n = 6). E. Immunostaining analysis of colonies formed by TICs with or without ectopic expression of active Yap1. TIC markers (p63 and Krt14) and NTC markers (Krt18). Laser confocal microscopy. F. Image histogram analysis of images from E. . Percentage of red Krt14+ cells was indicated (*, p < 0.01, n = 15).

    Article Snippet: Primary antibodies used in current study included anti-mouse Yap1 (Abcam, #ab56701), TAZ (Cell signaling, #4883), Histone H4 (Santa Cruz, #sc-25260), Hsp90 (Santa Cruz, #sc-8262), p-Smad2 (Santa Cruz, #sc-101801), Smad3 (Cell signaling, #9513), p-Smad3 (Santa Cruz, #sc-11769), Krt14 (Santa Cruz, #sc-43310), Krt18 (Santa Cruz, #sc-45406) and p63 (Abcam, #ab124762).

    Techniques: Transfection, Western Blot, Plasmid Preparation, Generated, Immunostaining, Expressing, Confocal Microscopy

    A. A FACS profile revealed a significant increase of TICs in Yap1 active tumors (86.7%) compared with empty vector transfected tumors (58.9%). B. Quantification of TIC ratio from A. (*, p = 0.0079, n = 13). C. Histologic analysis of mammary tumors with/without ectopic expression of active Yap1. Hematoxylin and Eosin (H&E) staining and immunostaining with TIC-marker (p63) and NTC marker (Krt18). D. In a transplanted tumor infected with active Yap1, confocal microscopy showed that a larger number of active Yap1 expression was co-localized with Krt14-positive cells. E. Colonies formed by breast TICs sorted from mammary tumors in A. with/without ectopic expression of active Yap1 ( p < 0.001, n = 6).

    Journal: Oncotarget

    Article Title: Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling

    doi: 10.18632/oncotarget.6655

    Figure Lengend Snippet: A. A FACS profile revealed a significant increase of TICs in Yap1 active tumors (86.7%) compared with empty vector transfected tumors (58.9%). B. Quantification of TIC ratio from A. (*, p = 0.0079, n = 13). C. Histologic analysis of mammary tumors with/without ectopic expression of active Yap1. Hematoxylin and Eosin (H&E) staining and immunostaining with TIC-marker (p63) and NTC marker (Krt18). D. In a transplanted tumor infected with active Yap1, confocal microscopy showed that a larger number of active Yap1 expression was co-localized with Krt14-positive cells. E. Colonies formed by breast TICs sorted from mammary tumors in A. with/without ectopic expression of active Yap1 ( p < 0.001, n = 6).

    Article Snippet: Primary antibodies used in current study included anti-mouse Yap1 (Abcam, #ab56701), TAZ (Cell signaling, #4883), Histone H4 (Santa Cruz, #sc-25260), Hsp90 (Santa Cruz, #sc-8262), p-Smad2 (Santa Cruz, #sc-101801), Smad3 (Cell signaling, #9513), p-Smad3 (Santa Cruz, #sc-11769), Krt14 (Santa Cruz, #sc-43310), Krt18 (Santa Cruz, #sc-45406) and p63 (Abcam, #ab124762).

    Techniques: Plasmid Preparation, Transfection, Expressing, Staining, Immunostaining, Marker, Infection, Confocal Microscopy

    A. Western blotting showed a less active form of Yap1 in the nucleus of Yap1-ko TICs than in parental TICs and Yap1-flp TICs. B. Genotyping of genomic DNA showed that the Yap1 gene was successfully knocked out of Yap1-ko mice with tamoxifen injection compared with Yap1-flp mice. C. In 2D culture, Giemsa staining and bright field imaging indicated a cell density originating from breast TICs sorted from mammary tumor with/without endogenous Yap1 deletion (Yap1-ko). D. In transplanted tumor withYap1-ko, confocal microscopy showed that less signal of active Yap1 was co-localized with Krt14-positive cells. E. Flow cytometry analysis of TIC fractions in mammary tumors after tamoxifen injection. TIC fractions were analyzed separately for GFP+ cells with Yap1 KO (Yap1-ko) and GFP- cells with functional endogenous Yap1 (Yap1-flp). F. Colonies formed by breast TICs sorted from mammary tumor in E. with/without Yap1 deletion (Yap1-ko). G. Relative cell numbers indicated cell growth in F. for mammary tumor cells with/without Yap1-ko (*, p = 0.006, n = 6).

    Journal: Oncotarget

    Article Title: Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling

    doi: 10.18632/oncotarget.6655

    Figure Lengend Snippet: A. Western blotting showed a less active form of Yap1 in the nucleus of Yap1-ko TICs than in parental TICs and Yap1-flp TICs. B. Genotyping of genomic DNA showed that the Yap1 gene was successfully knocked out of Yap1-ko mice with tamoxifen injection compared with Yap1-flp mice. C. In 2D culture, Giemsa staining and bright field imaging indicated a cell density originating from breast TICs sorted from mammary tumor with/without endogenous Yap1 deletion (Yap1-ko). D. In transplanted tumor withYap1-ko, confocal microscopy showed that less signal of active Yap1 was co-localized with Krt14-positive cells. E. Flow cytometry analysis of TIC fractions in mammary tumors after tamoxifen injection. TIC fractions were analyzed separately for GFP+ cells with Yap1 KO (Yap1-ko) and GFP- cells with functional endogenous Yap1 (Yap1-flp). F. Colonies formed by breast TICs sorted from mammary tumor in E. with/without Yap1 deletion (Yap1-ko). G. Relative cell numbers indicated cell growth in F. for mammary tumor cells with/without Yap1-ko (*, p = 0.006, n = 6).

    Article Snippet: Primary antibodies used in current study included anti-mouse Yap1 (Abcam, #ab56701), TAZ (Cell signaling, #4883), Histone H4 (Santa Cruz, #sc-25260), Hsp90 (Santa Cruz, #sc-8262), p-Smad2 (Santa Cruz, #sc-101801), Smad3 (Cell signaling, #9513), p-Smad3 (Santa Cruz, #sc-11769), Krt14 (Santa Cruz, #sc-43310), Krt18 (Santa Cruz, #sc-45406) and p63 (Abcam, #ab124762).

    Techniques: Western Blot, Injection, Staining, Imaging, Confocal Microscopy, Flow Cytometry, Functional Assay