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mrpa  (ATCC)


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    ATCC mrpa
    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
    Mrpa, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers"

    Article Title: An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2024.11.029

    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
    Figure Legend Snippet: Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

    Techniques Used: Zeta Potential Analyzer, Incubation, Staining



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    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
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    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
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    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
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    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects <t>against</t> <t>MRSA</t> (K) and <t>MRPA</t> (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.
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    Image Search Results


    Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

    Journal: Bioactive Materials

    Article Title: An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers

    doi: 10.1016/j.bioactmat.2024.11.029

    Figure Lengend Snippet: Examining the IZPP nanoparticles' physical, chemical, and biological attributes. (A) TEM and SEM images of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (B) Representative image of IZPP captured through TEM, along with its elemental mapping (HAADF: high-angle annular dark field). (C) Zeta potential of IAA/ZIF-8, IAA/ZIF-8@PDA and IZPP. (D) SEM image of IZPP after 5 mM ATP incubation for 10 min. (E) Chromatographic profile of the reaction's output post-incubation of either HRP or IZPP with IAA. (F) Illustrative schematic showing IZPP's ROS generation via the TMB colorimetric process. (G) UV–VIS–NIR spectral analysis with corresponding images of TMB mixtures reacted with various samples for 15 min in ATP's presence. (H) UV–VIS–NIR spectra of TMB for varying IZPP concentrations with ATP present. (I and J) Flow cytometric characterization of DCFH-DA stained RAW 264.7 (I) and RS1 (J) cells following varied IZPP exposures with ATP. (K and L) Comparative study of antibacterial effects against MRSA (K) and MRPA (L) across different sample groups. Error bars denote mean ± SD (n = 6). ∗∗∗ signifies P < 0.001.

    Article Snippet: We selected MRSA (ATCC 43310) and MRPA (ATCC 27853) as test bacteria.

    Techniques: Zeta Potential Analyzer, Incubation, Staining