451 orfc4 a sequence 3 ct a ag a  (ATCC)


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    Structured Review

    ATCC 451 orfc4 a sequence 3 ct a ag a
    451 Orfc4 A Sequence 3 Ct A Ag A, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/451 orfc4 a sequence 3 ct a ag a/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    451 orfc4 a sequence 3 ct a ag a - by Bioz Stars, 2024-05
    85/100 stars

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    451 orfc4 a sequence 3 ct a ag a  (ATCC)


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    Structured Review

    ATCC 451 orfc4 a sequence 3 ct a ag a
    451 Orfc4 A Sequence 3 Ct A Ag A, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/451 orfc4 a sequence 3 ct a ag a/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    451 orfc4 a sequence 3 ct a ag a - by Bioz Stars, 2024-05
    85/100 stars

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    451 orfc4 a sequence 3 ct  (ATCC)


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    Structured Review

    ATCC 451 orfc4 a sequence 3 ct
    Primers used in the study a
    451 Orfc4 A Sequence 3 Ct, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/451 orfc4 a sequence 3 ct/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    451 orfc4 a sequence 3 ct - by Bioz Stars, 2024-05
    85/100 stars

    Images

    1) Product Images from "Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples"

    Article Title: Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02305-09

    Primers used in the study a
    Figure Legend Snippet: Primers used in the study a

    Techniques Used: Sequencing, Binding Assay, Amplification

    Schematic representation of the amplification curves (left), melting curves (middle), and electrophoretic separation of products (right) obtained using the gdh, orfC4, and tpi qPCR assays. In gel electrophoresis of gdh products, lane M is the 50-bp size ladder; lanes 1 and 6 show the assemblage A product (180 bp); lanes 2, 3, and 7 show the assemblage B product (133 bp); lanes 4, 5, and 8 show the presence of both assemblages A and B; and lane 9 shows the negative control. In gel electrophoresis of orfC4 products, lane M is the 50-bp size ladder; lanes 1 and 7 show the assemblage A product (103 bp); lanes 3, 4, 5, and 8 show the assemblage B product (171 bp); lanes 2, 6, and 9 show the presence of both assemblages A and B; and lane 10 shows the negative control. In gel electrophoresis of tpi amplicons, the product from assemblage A is not cut by AluI (lane 1) but is cut by HincII into two fragments of 47 bp and 31 bp (lane 2), whereas the B product is cut by AluI into two fragments of 45 bp and 32 bp (lane 3) but is not cut by HincII (lane 4).
    Figure Legend Snippet: Schematic representation of the amplification curves (left), melting curves (middle), and electrophoretic separation of products (right) obtained using the gdh, orfC4, and tpi qPCR assays. In gel electrophoresis of gdh products, lane M is the 50-bp size ladder; lanes 1 and 6 show the assemblage A product (180 bp); lanes 2, 3, and 7 show the assemblage B product (133 bp); lanes 4, 5, and 8 show the presence of both assemblages A and B; and lane 9 shows the negative control. In gel electrophoresis of orfC4 products, lane M is the 50-bp size ladder; lanes 1 and 7 show the assemblage A product (103 bp); lanes 3, 4, 5, and 8 show the assemblage B product (171 bp); lanes 2, 6, and 9 show the presence of both assemblages A and B; and lane 10 shows the negative control. In gel electrophoresis of tpi amplicons, the product from assemblage A is not cut by AluI (lane 1) but is cut by HincII into two fragments of 47 bp and 31 bp (lane 2), whereas the B product is cut by AluI into two fragments of 45 bp and 32 bp (lane 3) but is not cut by HincII (lane 4).

    Techniques Used: Amplification, Nucleic Acid Electrophoresis, Negative Control

    Genotyping results obtained for 30 human samples at four loci using regular PCR and qPCR on genomic DNA from stools, on purified cysts, and on single cysts
    Figure Legend Snippet: Genotyping results obtained for 30 human samples at four loci using regular PCR and qPCR on genomic DNA from stools, on purified cysts, and on single cysts

    Techniques Used: Purification

    451 orfc4 a sequence 3 ct  (ATCC)


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    Structured Review

    ATCC 451 orfc4 a sequence 3 ct
    Primers used in the study a
    451 Orfc4 A Sequence 3 Ct, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/451 orfc4 a sequence 3 ct/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    451 orfc4 a sequence 3 ct - by Bioz Stars, 2024-05
    85/100 stars

    Images

    1) Product Images from "Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples "

    Article Title: Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02305-09

    Primers used in the study a
    Figure Legend Snippet: Primers used in the study a

    Techniques Used: Sequencing, Binding Assay, Amplification

    Schematic representation of the amplification curves (left), melting curves (middle), and electrophoretic separation of products (right) obtained using the gdh, orfC4, and tpi qPCR assays. In gel electrophoresis of gdh products, lane M is the 50-bp size ladder; lanes 1 and 6 show the assemblage A product (180 bp); lanes 2, 3, and 7 show the assemblage B product (133 bp); lanes 4, 5, and 8 show the presence of both assemblages A and B; and lane 9 shows the negative control. In gel electrophoresis of orfC4 products, lane M is the 50-bp size ladder; lanes 1 and 7 show the assemblage A product (103 bp); lanes 3, 4, 5, and 8 show the assemblage B product (171 bp); lanes 2, 6, and 9 show the presence of both assemblages A and B; and lane 10 shows the negative control. In gel electrophoresis of tpi amplicons, the product from assemblage A is not cut by AluI (lane 1) but is cut by HincII into two fragments of 47 bp and 31 bp (lane 2), whereas the B product is cut by AluI into two fragments of 45 bp and 32 bp (lane 3) but is not cut by HincII (lane 4).
    Figure Legend Snippet: Schematic representation of the amplification curves (left), melting curves (middle), and electrophoretic separation of products (right) obtained using the gdh, orfC4, and tpi qPCR assays. In gel electrophoresis of gdh products, lane M is the 50-bp size ladder; lanes 1 and 6 show the assemblage A product (180 bp); lanes 2, 3, and 7 show the assemblage B product (133 bp); lanes 4, 5, and 8 show the presence of both assemblages A and B; and lane 9 shows the negative control. In gel electrophoresis of orfC4 products, lane M is the 50-bp size ladder; lanes 1 and 7 show the assemblage A product (103 bp); lanes 3, 4, 5, and 8 show the assemblage B product (171 bp); lanes 2, 6, and 9 show the presence of both assemblages A and B; and lane 10 shows the negative control. In gel electrophoresis of tpi amplicons, the product from assemblage A is not cut by AluI (lane 1) but is cut by HincII into two fragments of 47 bp and 31 bp (lane 2), whereas the B product is cut by AluI into two fragments of 45 bp and 32 bp (lane 3) but is not cut by HincII (lane 4).

    Techniques Used: Amplification, Nucleic Acid Electrophoresis, Negative Control

    Genotyping results obtained for 30 human samples at four loci using regular PCR and qPCR on genomic DNA from stools, on purified cysts, and on single cysts
    Figure Legend Snippet: Genotyping results obtained for 30 human samples at four loci using regular PCR and qPCR on genomic DNA from stools, on purified cysts, and on single cysts

    Techniques Used: Purification

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    ATCC 451 orfc4 a sequence 3 ct a ag a
    451 Orfc4 A Sequence 3 Ct A Ag A, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/451 orfc4 a sequence 3 ct a ag a/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    451 orfc4 a sequence 3 ct a ag a - by Bioz Stars, 2024-05
    85/100 stars
      Buy from Supplier

    85
    ATCC 451 orfc4 a sequence 3 ct
    Primers used in the study a
    451 Orfc4 A Sequence 3 Ct, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/451 orfc4 a sequence 3 ct/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    451 orfc4 a sequence 3 ct - by Bioz Stars, 2024-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    Primers used in the study a

    Journal: Applied and Environmental Microbiology

    Article Title: Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples

    doi: 10.1128/AEM.02305-09

    Figure Lengend Snippet: Primers used in the study a

    Article Snippet: The primers are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Primer Sequence Binding site Amplicon size (bp) Melting peak (°C) TPI A forward primer 5′- TCGTCATTGCCCCTTCC GCC -3′ 116-135 77 86 TPI B sequence 5′- .T..TG....T..C... TTT -3′ TPI A reverse primer 3′- CAGT TGAGGATAGCAGCG -5′ 175-192 TPI B sequence 3′- TGTC ...AA......... -5′ TPI B forward primer 5′- GATGAACGCAAGGCCAA TAA -3′ 397-416 77 80 TPI A sequence 5′- ..C..G........... CCG -3′ TPI B reverse primer 3′- AAG AAGGAGATTGGAGAATC -5′ 454-473 TPI A sequence 3′- GGC ......C.C.....G.. -5′ GDH A forward primer 5′- CCGGCAACGTTGCCCAGT TT -3′ 710-729 180 83 GDH B sequence 5′- .T...........T.... AC -3′ GDH A reverse primer 3′- T CCGAGTTCAAGGACAAGT -5′ 871-889 GDH B sequence 3′- G .T...........G.... -5′ GDH B forward primer 5′- CGTATTGGCGTCGGCG GT -3′ 492-510 133 83 GDH A sequence 5′- ...C..C.......... CC -3′ GDH B reverse primer 3′- C TATCAGACCAGAGGCCACA -5′ 604-623 GDH A sequence 3′- T ..........G........G -5′ ORFC4 A forward primer 5′- CTGTAGACAGGGCCCAG GCC -3′ 135-154 103 85 ORFC4 B sequence 5′- ......G....AG..G. ATG -3′ ORFC4 A reverse primer 3′- ATT AAGGGCAGGGGACATCAT -5′ 217-237 ORFC4 B sequence 3′- GGC ...T.T.A.....G.... -5′ ORFC4 B forward primer 5′- ACTGTCCATTTCTATC TGAG -3′ 281-300 171 79 ORFC4 A sequence 5′- C...C........G.. ATCC -3′ ORFC4 B reverse primer 3′- AG GTGGAGGCCAATGGAATCC -5′ 431-451 ORFC4 A sequence 3′- CT ..A.....AG........A -5′ Open in a separate window a The sequence of each assemblage-specific primer is aligned over the corresponding sequence of the other assemblage.

    Techniques: Sequencing, Binding Assay, Amplification

    Schematic representation of the amplification curves (left), melting curves (middle), and electrophoretic separation of products (right) obtained using the gdh, orfC4, and tpi qPCR assays. In gel electrophoresis of gdh products, lane M is the 50-bp size ladder; lanes 1 and 6 show the assemblage A product (180 bp); lanes 2, 3, and 7 show the assemblage B product (133 bp); lanes 4, 5, and 8 show the presence of both assemblages A and B; and lane 9 shows the negative control. In gel electrophoresis of orfC4 products, lane M is the 50-bp size ladder; lanes 1 and 7 show the assemblage A product (103 bp); lanes 3, 4, 5, and 8 show the assemblage B product (171 bp); lanes 2, 6, and 9 show the presence of both assemblages A and B; and lane 10 shows the negative control. In gel electrophoresis of tpi amplicons, the product from assemblage A is not cut by AluI (lane 1) but is cut by HincII into two fragments of 47 bp and 31 bp (lane 2), whereas the B product is cut by AluI into two fragments of 45 bp and 32 bp (lane 3) but is not cut by HincII (lane 4).

    Journal: Applied and Environmental Microbiology

    Article Title: Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples

    doi: 10.1128/AEM.02305-09

    Figure Lengend Snippet: Schematic representation of the amplification curves (left), melting curves (middle), and electrophoretic separation of products (right) obtained using the gdh, orfC4, and tpi qPCR assays. In gel electrophoresis of gdh products, lane M is the 50-bp size ladder; lanes 1 and 6 show the assemblage A product (180 bp); lanes 2, 3, and 7 show the assemblage B product (133 bp); lanes 4, 5, and 8 show the presence of both assemblages A and B; and lane 9 shows the negative control. In gel electrophoresis of orfC4 products, lane M is the 50-bp size ladder; lanes 1 and 7 show the assemblage A product (103 bp); lanes 3, 4, 5, and 8 show the assemblage B product (171 bp); lanes 2, 6, and 9 show the presence of both assemblages A and B; and lane 10 shows the negative control. In gel electrophoresis of tpi amplicons, the product from assemblage A is not cut by AluI (lane 1) but is cut by HincII into two fragments of 47 bp and 31 bp (lane 2), whereas the B product is cut by AluI into two fragments of 45 bp and 32 bp (lane 3) but is not cut by HincII (lane 4).

    Article Snippet: The primers are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Primer Sequence Binding site Amplicon size (bp) Melting peak (°C) TPI A forward primer 5′- TCGTCATTGCCCCTTCC GCC -3′ 116-135 77 86 TPI B sequence 5′- .T..TG....T..C... TTT -3′ TPI A reverse primer 3′- CAGT TGAGGATAGCAGCG -5′ 175-192 TPI B sequence 3′- TGTC ...AA......... -5′ TPI B forward primer 5′- GATGAACGCAAGGCCAA TAA -3′ 397-416 77 80 TPI A sequence 5′- ..C..G........... CCG -3′ TPI B reverse primer 3′- AAG AAGGAGATTGGAGAATC -5′ 454-473 TPI A sequence 3′- GGC ......C.C.....G.. -5′ GDH A forward primer 5′- CCGGCAACGTTGCCCAGT TT -3′ 710-729 180 83 GDH B sequence 5′- .T...........T.... AC -3′ GDH A reverse primer 3′- T CCGAGTTCAAGGACAAGT -5′ 871-889 GDH B sequence 3′- G .T...........G.... -5′ GDH B forward primer 5′- CGTATTGGCGTCGGCG GT -3′ 492-510 133 83 GDH A sequence 5′- ...C..C.......... CC -3′ GDH B reverse primer 3′- C TATCAGACCAGAGGCCACA -5′ 604-623 GDH A sequence 3′- T ..........G........G -5′ ORFC4 A forward primer 5′- CTGTAGACAGGGCCCAG GCC -3′ 135-154 103 85 ORFC4 B sequence 5′- ......G....AG..G. ATG -3′ ORFC4 A reverse primer 3′- ATT AAGGGCAGGGGACATCAT -5′ 217-237 ORFC4 B sequence 3′- GGC ...T.T.A.....G.... -5′ ORFC4 B forward primer 5′- ACTGTCCATTTCTATC TGAG -3′ 281-300 171 79 ORFC4 A sequence 5′- C...C........G.. ATCC -3′ ORFC4 B reverse primer 3′- AG GTGGAGGCCAATGGAATCC -5′ 431-451 ORFC4 A sequence 3′- CT ..A.....AG........A -5′ Open in a separate window a The sequence of each assemblage-specific primer is aligned over the corresponding sequence of the other assemblage.

    Techniques: Amplification, Nucleic Acid Electrophoresis, Negative Control

    Genotyping results obtained for 30 human samples at four loci using regular PCR and qPCR on genomic DNA from stools, on purified cysts, and on single cysts

    Journal: Applied and Environmental Microbiology

    Article Title: Genotyping of Giardia duodenalis Cysts by New Real-Time PCR Assays for Detection of Mixed Infections in Human Samples

    doi: 10.1128/AEM.02305-09

    Figure Lengend Snippet: Genotyping results obtained for 30 human samples at four loci using regular PCR and qPCR on genomic DNA from stools, on purified cysts, and on single cysts

    Article Snippet: The primers are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Primer Sequence Binding site Amplicon size (bp) Melting peak (°C) TPI A forward primer 5′- TCGTCATTGCCCCTTCC GCC -3′ 116-135 77 86 TPI B sequence 5′- .T..TG....T..C... TTT -3′ TPI A reverse primer 3′- CAGT TGAGGATAGCAGCG -5′ 175-192 TPI B sequence 3′- TGTC ...AA......... -5′ TPI B forward primer 5′- GATGAACGCAAGGCCAA TAA -3′ 397-416 77 80 TPI A sequence 5′- ..C..G........... CCG -3′ TPI B reverse primer 3′- AAG AAGGAGATTGGAGAATC -5′ 454-473 TPI A sequence 3′- GGC ......C.C.....G.. -5′ GDH A forward primer 5′- CCGGCAACGTTGCCCAGT TT -3′ 710-729 180 83 GDH B sequence 5′- .T...........T.... AC -3′ GDH A reverse primer 3′- T CCGAGTTCAAGGACAAGT -5′ 871-889 GDH B sequence 3′- G .T...........G.... -5′ GDH B forward primer 5′- CGTATTGGCGTCGGCG GT -3′ 492-510 133 83 GDH A sequence 5′- ...C..C.......... CC -3′ GDH B reverse primer 3′- C TATCAGACCAGAGGCCACA -5′ 604-623 GDH A sequence 3′- T ..........G........G -5′ ORFC4 A forward primer 5′- CTGTAGACAGGGCCCAG GCC -3′ 135-154 103 85 ORFC4 B sequence 5′- ......G....AG..G. ATG -3′ ORFC4 A reverse primer 3′- ATT AAGGGCAGGGGACATCAT -5′ 217-237 ORFC4 B sequence 3′- GGC ...T.T.A.....G.... -5′ ORFC4 B forward primer 5′- ACTGTCCATTTCTATC TGAG -3′ 281-300 171 79 ORFC4 A sequence 5′- C...C........G.. ATCC -3′ ORFC4 B reverse primer 3′- AG GTGGAGGCCAATGGAATCC -5′ 431-451 ORFC4 A sequence 3′- CT ..A.....AG........A -5′ Open in a separate window a The sequence of each assemblage-specific primer is aligned over the corresponding sequence of the other assemblage.

    Techniques: Purification