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n gonorrhoeae atcc 43070  (ATCC)


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    Structured Review

    ATCC n gonorrhoeae atcc 43070
    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae <t>ATCC</t> <t>43070</t> (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
    N Gonorrhoeae Atcc 43070, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "The Human Microbiome as a Focus of Antibiotic Discovery: Neisseria mucosa Displays Activity Against Neisseria gonorrhoeae"

    Article Title: The Human Microbiome as a Focus of Antibiotic Discovery: Neisseria mucosa Displays Activity Against Neisseria gonorrhoeae

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.577762

    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae ATCC 43070 (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
    Figure Legend Snippet: Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae ATCC 43070 (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.

    Techniques Used: Amplification, Overlay Assay, Purification



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    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae <t>ATCC</t> <t>43070</t> (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
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    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae <t>ATCC</t> <t>43070</t> (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
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    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae <t>ATCC</t> <t>43070</t> (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
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    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae <t>ATCC</t> <t>43070</t> (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
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    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae <t>ATCC</t> <t>43070</t> (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.
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    Image Search Results


    Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae ATCC 43070 (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.

    Journal: Frontiers in Microbiology

    Article Title: The Human Microbiome as a Focus of Antibiotic Discovery: Neisseria mucosa Displays Activity Against Neisseria gonorrhoeae

    doi: 10.3389/fmicb.2020.577762

    Figure Lengend Snippet: Project design and example data from each project phase. Undergraduate involvement in each phase is given in shaded boxes. (A) Products of rplF amplification. Left to right: size standard (bottom to top: 300, 400, 500, and 750 bp), PCR products from N. mucosa NRL 9300, N. mucosa NRL 9297, N. mucosa ATCC 25996, N. sicca NRL 30016, and N. sicca ATCC 29256. (B) Cross streak assay of N. mucosa NRL 9300 inhibiting N. flavescens N47 (left of center) and N. gonorrhoeae ATCC 43070 (right of center), n = 3. (C) Agar overlay assay of a colony of N. mucosa NRL 9300 inhibiting the growth of a lawn of N. flavescens N47, n = 3. (D) Agar overlay assay of a purified ethyl acetate extract prepared from N. mucosa NRL 9300 inhibiting the growth of a lawn of N. gonorrhoeae FA6140, n = 5. (E) Control agar overlay assay of an ethyl acetate extract prepared from uninoculated GCB agar tested against a lawn of N. gonorrhoeae FA6140, n = 5.

    Article Snippet: A semiquantitative cross-streak assay was used to screen all 36 isolates against three target strains: N. lactamica ATCC 23970, N. flavescens N47, and N. gonorrhoeae ATCC 43070.

    Techniques: Amplification, Overlay Assay, Purification