Journal: PLoS ONE

Article Title: Bacterial Viability and Physical Properties of Antibacterially Modified Experimental Dental Resin Composites

doi: 10.1371/journal.pone.0079119

Figure Lengend Snippet: Means and (standard deviations) of total, vital bacterial and non-vital bacterial pixel-count after 8 h and 24 h observation time.

Article Snippet: Streptococcus sanguinis (strain 20068), Streptococcus oralis (strain 20627, Streptococcus mitis (strain 12643), Actinomyces viscosus (strain 43329), and Actinomyces naeslundii (strain 17233, all strains from DSMZ GmbH, Braunschweig, Germany) were used to determine cell viability.

Techniques:

Journal: Microorganisms

Article Title: Root Caries Preventive Effect of Varnishes Containing Fluoride or Fluoride + Chlorhexidine/Cetylpyridinium Chloride In Vitro

doi: 10.3390/microorganisms9040737

Figure Lengend Snippet: Artificial mouth model setting for the cariogenic biofilm challenge. Time for provision of the different media within a 24 h cycle is presented. Abbreviations: SM: S. mutans , LR: L. rhamnosus , AN: A. naeslundii , BHI: brain heart infusion, DMM: defined mucin medium.

Article Snippet: Three cariogenic bacterial strains, Lactobacillus rhamnosus (LR) (DSM 20021), Streptococcus mutans (SM) (DSM 20523) and Actinomyces naeslundii (AN) (DSM 43013) (DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) were used to create cariogenic biofilms.

Techniques:

Journal: Microorganisms

Article Title: Root Caries Preventive Effect of Varnishes Containing Fluoride or Fluoride + Chlorhexidine/Cetylpyridinium Chloride In Vitro

doi: 10.3390/microorganisms9040737

Figure Lengend Snippet: Bacterial counts (CFU/mL) at 10 5 dilution of the different bacteria strains. Medians, quartiles and extreme values are given. Box plots denoted with the same letter (SM: lower case letters, LR: upper case letters, AN: underlined letters) do not differ significantly (ANOVA/Tukey HSD); Abbreviations: SM: S. mutans , LR: L. rhamnosus , AN: A. naeslundii , F: Fluoride varnish, F-P: Placebo varnish for F, CF: Fluoride + CHX varnish, CF-P: Placebo varnish for CF, C: Control group.

Article Snippet: Three cariogenic bacterial strains, Lactobacillus rhamnosus (LR) (DSM 20021), Streptococcus mutans (SM) (DSM 20523) and Actinomyces naeslundii (AN) (DSM 43013) (DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) were used to create cariogenic biofilms.

Techniques:

Journal: Microorganisms

Article Title: Root Caries Preventive Effect of Varnishes Containing Fluoride or Fluoride + Chlorhexidine/Cetylpyridinium Chloride In Vitro

doi: 10.3390/microorganisms9040737

Figure Lengend Snippet: Fluorescence in situ hybridization (FISH) of control biofilms. FISH demonstrated the presence of relatively thick (>200 µm) biofilms comprising all three employed bacterial strains. Control biofilms were dominated by large clusters of S. mutans (SM) (green/turquoise) and L. rhamnosus (LR) (blue), with smaller colonies of A. naeslundii (AN) (red) ( A , B ). Both LR ( B ) and SM ( B , C ), but not AN penetrated dentinal tubules, with a penetration depth of up to 100 µm in some areas ( B , C ). Bars = 20 µm.

Article Snippet: Three cariogenic bacterial strains, Lactobacillus rhamnosus (LR) (DSM 20021), Streptococcus mutans (SM) (DSM 20523) and Actinomyces naeslundii (AN) (DSM 43013) (DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) were used to create cariogenic biofilms.

Techniques: Fluorescence, In Situ Hybridization

Journal: Microorganisms

Article Title: Root Caries Preventive Effect of Varnishes Containing Fluoride or Fluoride + Chlorhexidine/Cetylpyridinium Chloride In Vitro

doi: 10.3390/microorganisms9040737

Figure Lengend Snippet: Fluorescence in situ hybridization (FISH) of biofilms treated with F ( A ) or CF ( B ). For both treatment groups, thick biofilms containing all employed strains were observed. Biofilms were dominated by L. rhamnosus (LR) (blue) and S. mutans (SM) (green/turquoise), with small, interspersed clusters of A. naeslundii (AN) (red). Interestingly, the basal layer of the biofilm on specimens treated with fluoride + CHX varnish contained only LR ( B ). The biofilms were firmly attached to the underlying dentin, but the layer of varnish prevented any bacterial penetration of dentinal tubules.

Article Snippet: Three cariogenic bacterial strains, Lactobacillus rhamnosus (LR) (DSM 20021), Streptococcus mutans (SM) (DSM 20523) and Actinomyces naeslundii (AN) (DSM 43013) (DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) were used to create cariogenic biofilms.

Techniques: Fluorescence, In Situ Hybridization