cbs  (ATCC)


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    ATCC cbs
    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia <t>atrofusca</t> <t>NRRL</t> 22316 and G. cicatricum <t>CBS</t> 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    Cbs, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91/100 stars

    Images

    1) Product Images from "Fusarium : more than a node or a foot-shaped basal cell"

    Article Title: Fusarium : more than a node or a foot-shaped basal cell

    Journal: Studies in Mycology

    doi: 10.1016/j.simyco.2021.100116

    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia atrofusca NRRL 22316 and G. cicatricum CBS 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    Figure Legend Snippet: Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia atrofusca NRRL 22316 and G. cicatricum CBS 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.

    Techniques Used: Sequencing

    cbs  (ATCC)


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    ATCC cbs
    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia <t>atrofusca</t> <t>NRRL</t> 22316 and G. cicatricum <t>CBS</t> 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    Cbs, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Fusarium : more than a node or a foot-shaped basal cell"

    Article Title: Fusarium : more than a node or a foot-shaped basal cell

    Journal: Studies in Mycology

    doi: 10.1016/j.simyco.2021.100116

    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia atrofusca NRRL 22316 and G. cicatricum CBS 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    Figure Legend Snippet: Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia atrofusca NRRL 22316 and G. cicatricum CBS 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.

    Techniques Used: Sequencing

    r erythropolis sq1 mutant  (ATCC)


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    ATCC r erythropolis sq1 mutant
    R Erythropolis Sq1 Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    r erythropolis sq1 mutant  (ATCC)


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    ATCC r erythropolis sq1 mutant
    Strains and plasmids used in this study
    R Erythropolis Sq1 Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid ? 1 -Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1"

    Article Title: Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid ? 1 -Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1

    Journal:

    doi:

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Mutagenesis, Expressing

    Cloning strategies for the construction of pSDH205 and the nonreplicative vector pSDH420 used for kstD targeted gene disruption. ‘kstD’ in pSDH420 indicates the internal 741-bp Asp718/SalI fragment of the kstD gene used for targeted gene disruption of kstD in R. erythropolis SQ1. A 1,756-bp ClaI/BamHI fragment of pSDH205 (sites marked with asterisks) was used for the construction of pSDH101 (for complementation of the kstD mutant phenotype [Table 1]) and pSDH305 (heterologous kstD expression) [Table 1]). ORFs are indicated with arrows. Solid curved lines represent R. erythropolis SQ1 DNA. Open curved lines represent regions of the Rhodococcus-E. coli shuttle vector pDA71, encoding replication in Rhodococcus (rep) and chloramphenicol resistance (cat).
    Figure Legend Snippet: Cloning strategies for the construction of pSDH205 and the nonreplicative vector pSDH420 used for kstD targeted gene disruption. ‘kstD’ in pSDH420 indicates the internal 741-bp Asp718/SalI fragment of the kstD gene used for targeted gene disruption of kstD in R. erythropolis SQ1. A 1,756-bp ClaI/BamHI fragment of pSDH205 (sites marked with asterisks) was used for the construction of pSDH101 (for complementation of the kstD mutant phenotype [Table 1]) and pSDH305 (heterologous kstD expression) [Table 1]). ORFs are indicated with arrows. Solid curved lines represent R. erythropolis SQ1 DNA. Open curved lines represent regions of the Rhodococcus-E. coli shuttle vector pDA71, encoding replication in Rhodococcus (rep) and chloramphenicol resistance (cat).

    Techniques Used: Clone Assay, Plasmid Preparation, Mutagenesis, Expressing

    Bioconversion rates and electrotransformation frequencies a of some phytosterol (Generol)-degrading Rhodococcus strains
    Figure Legend Snippet: Bioconversion rates and electrotransformation frequencies a of some phytosterol (Generol)-degrading Rhodococcus strains

    Techniques Used: Transformation Assay

    Schematic overview of targeted gene disruption of kstD by integration of pSDH420 into the R. erythropolis SQ1 chromosome. Single homologous recombination between the wild-type kstD gene on the R. erythropolis SQ1 chromosome and a 741-bp Asp718/SalI internal kstD fragment located on pSDH420 divides the wild-type ClaI DNA fragment (2,095 bp) into two ClaI DNA fragments of 1.06 and 6.05 kb. ORF2, encoding a TetR type of repressor protein, was identified upstream of kstD. Forward and reverse kstD primers, used for screening of potential kstD gene disruption mutants with colony PCR, are indicated by arrows.
    Figure Legend Snippet: Schematic overview of targeted gene disruption of kstD by integration of pSDH420 into the R. erythropolis SQ1 chromosome. Single homologous recombination between the wild-type kstD gene on the R. erythropolis SQ1 chromosome and a 741-bp Asp718/SalI internal kstD fragment located on pSDH420 divides the wild-type ClaI DNA fragment (2,095 bp) into two ClaI DNA fragments of 1.06 and 6.05 kb. ORF2, encoding a TetR type of repressor protein, was identified upstream of kstD. Forward and reverse kstD primers, used for screening of potential kstD gene disruption mutants with colony PCR, are indicated by arrows.

    Techniques Used: Homologous Recombination

    Southern analysis of genomic DNA digested with ClaI of three individual kstD mutants (lanes 1 to 3) and wild-type R. erythropolis SQ1 (lane 4). The complete kstD gene, obtained with PCR using forward and reverse kstD primers, was used as a DIG-labeled probe.
    Figure Legend Snippet: Southern analysis of genomic DNA digested with ClaI of three individual kstD mutants (lanes 1 to 3) and wild-type R. erythropolis SQ1 (lane 4). The complete kstD gene, obtained with PCR using forward and reverse kstD primers, was used as a DIG-labeled probe.

    Techniques Used: Labeling

    KSTD activity staining, using 4-androstene-3,17-dione as a substrate, on native PAGE gels loaded with cell extracts (approximately 35 μg of protein) of noninduced wild-type R. erythropolis SQ1 (lane 1), 4-androstene-3,17-dione-induced wild-type R. erythropolis SQ1 (lane 2), 4-androstene-3,17-dione-induced SDH420 (a kstD mutant) (lane 3), E. coli DH5α/pBlueScript(II) KS (lane 4), and E. coli DH5α/pSDH305 (lane 5). No activity bands could be visualized in controls using 1,4-androstadiene-3,17-dione as a substrate for staining.
    Figure Legend Snippet: KSTD activity staining, using 4-androstene-3,17-dione as a substrate, on native PAGE gels loaded with cell extracts (approximately 35 μg of protein) of noninduced wild-type R. erythropolis SQ1 (lane 1), 4-androstene-3,17-dione-induced wild-type R. erythropolis SQ1 (lane 2), 4-androstene-3,17-dione-induced SDH420 (a kstD mutant) (lane 3), E. coli DH5α/pBlueScript(II) KS (lane 4), and E. coli DH5α/pSDH305 (lane 5). No activity bands could be visualized in controls using 1,4-androstadiene-3,17-dione as a substrate for staining.

    Techniques Used: Activity Assay, Staining, Clear Native PAGE, Mutagenesis

    r erythropolis sq1 mutant  (ATCC)


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    ATCC r erythropolis sq1 mutant
    Strains and plasmids used in this study
    R Erythropolis Sq1 Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r erythropolis sq1 mutant/product/ATCC
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    Images

    1) Product Images from "Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid ? 1 -Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1"

    Article Title: Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid ? 1 -Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1

    Journal:

    doi:

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Mutagenesis, Expressing

    Cloning strategies for the construction of pSDH205 and the nonreplicative vector pSDH420 used for kstD targeted gene disruption. ‘kstD’ in pSDH420 indicates the internal 741-bp Asp718/SalI fragment of the kstD gene used for targeted gene disruption of kstD in R. erythropolis SQ1. A 1,756-bp ClaI/BamHI fragment of pSDH205 (sites marked with asterisks) was used for the construction of pSDH101 (for complementation of the kstD mutant phenotype [Table 1]) and pSDH305 (heterologous kstD expression) [Table 1]). ORFs are indicated with arrows. Solid curved lines represent R. erythropolis SQ1 DNA. Open curved lines represent regions of the Rhodococcus-E. coli shuttle vector pDA71, encoding replication in Rhodococcus (rep) and chloramphenicol resistance (cat).
    Figure Legend Snippet: Cloning strategies for the construction of pSDH205 and the nonreplicative vector pSDH420 used for kstD targeted gene disruption. ‘kstD’ in pSDH420 indicates the internal 741-bp Asp718/SalI fragment of the kstD gene used for targeted gene disruption of kstD in R. erythropolis SQ1. A 1,756-bp ClaI/BamHI fragment of pSDH205 (sites marked with asterisks) was used for the construction of pSDH101 (for complementation of the kstD mutant phenotype [Table 1]) and pSDH305 (heterologous kstD expression) [Table 1]). ORFs are indicated with arrows. Solid curved lines represent R. erythropolis SQ1 DNA. Open curved lines represent regions of the Rhodococcus-E. coli shuttle vector pDA71, encoding replication in Rhodococcus (rep) and chloramphenicol resistance (cat).

    Techniques Used: Clone Assay, Plasmid Preparation, Mutagenesis, Expressing

    Bioconversion rates and electrotransformation frequencies a of some phytosterol (Generol)-degrading Rhodococcus strains
    Figure Legend Snippet: Bioconversion rates and electrotransformation frequencies a of some phytosterol (Generol)-degrading Rhodococcus strains

    Techniques Used: Transformation Assay

    Schematic overview of targeted gene disruption of kstD by integration of pSDH420 into the R. erythropolis SQ1 chromosome. Single homologous recombination between the wild-type kstD gene on the R. erythropolis SQ1 chromosome and a 741-bp Asp718/SalI internal kstD fragment located on pSDH420 divides the wild-type ClaI DNA fragment (2,095 bp) into two ClaI DNA fragments of 1.06 and 6.05 kb. ORF2, encoding a TetR type of repressor protein, was identified upstream of kstD. Forward and reverse kstD primers, used for screening of potential kstD gene disruption mutants with colony PCR, are indicated by arrows.
    Figure Legend Snippet: Schematic overview of targeted gene disruption of kstD by integration of pSDH420 into the R. erythropolis SQ1 chromosome. Single homologous recombination between the wild-type kstD gene on the R. erythropolis SQ1 chromosome and a 741-bp Asp718/SalI internal kstD fragment located on pSDH420 divides the wild-type ClaI DNA fragment (2,095 bp) into two ClaI DNA fragments of 1.06 and 6.05 kb. ORF2, encoding a TetR type of repressor protein, was identified upstream of kstD. Forward and reverse kstD primers, used for screening of potential kstD gene disruption mutants with colony PCR, are indicated by arrows.

    Techniques Used: Homologous Recombination

    Southern analysis of genomic DNA digested with ClaI of three individual kstD mutants (lanes 1 to 3) and wild-type R. erythropolis SQ1 (lane 4). The complete kstD gene, obtained with PCR using forward and reverse kstD primers, was used as a DIG-labeled probe.
    Figure Legend Snippet: Southern analysis of genomic DNA digested with ClaI of three individual kstD mutants (lanes 1 to 3) and wild-type R. erythropolis SQ1 (lane 4). The complete kstD gene, obtained with PCR using forward and reverse kstD primers, was used as a DIG-labeled probe.

    Techniques Used: Labeling

    KSTD activity staining, using 4-androstene-3,17-dione as a substrate, on native PAGE gels loaded with cell extracts (approximately 35 μg of protein) of noninduced wild-type R. erythropolis SQ1 (lane 1), 4-androstene-3,17-dione-induced wild-type R. erythropolis SQ1 (lane 2), 4-androstene-3,17-dione-induced SDH420 (a kstD mutant) (lane 3), E. coli DH5α/pBlueScript(II) KS (lane 4), and E. coli DH5α/pSDH305 (lane 5). No activity bands could be visualized in controls using 1,4-androstadiene-3,17-dione as a substrate for staining.
    Figure Legend Snippet: KSTD activity staining, using 4-androstene-3,17-dione as a substrate, on native PAGE gels loaded with cell extracts (approximately 35 μg of protein) of noninduced wild-type R. erythropolis SQ1 (lane 1), 4-androstene-3,17-dione-induced wild-type R. erythropolis SQ1 (lane 2), 4-androstene-3,17-dione-induced SDH420 (a kstD mutant) (lane 3), E. coli DH5α/pBlueScript(II) KS (lane 4), and E. coli DH5α/pSDH305 (lane 5). No activity bands could be visualized in controls using 1,4-androstadiene-3,17-dione as a substrate for staining.

    Techniques Used: Activity Assay, Staining, Clear Native PAGE, Mutagenesis

    derivative  (ATCC)


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    ATCC derivative
    Derivative, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    derivative - by Bioz Stars, 2024-02
    91/100 stars

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  • cbs  (ATCC)
    91
    ATCC cbs
    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia <t>atrofusca</t> <t>NRRL</t> 22316 and G. cicatricum <t>CBS</t> 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    Cbs, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cbs/product/ATCC
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cbs - by Bioz Stars, 2024-02
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    91
    ATCC r erythropolis sq1 mutant
    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia <t>atrofusca</t> <t>NRRL</t> 22316 and G. cicatricum <t>CBS</t> 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    R Erythropolis Sq1 Mutant, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r erythropolis sq1 mutant/product/ATCC
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r erythropolis sq1 mutant - by Bioz Stars, 2024-02
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    91
    ATCC derivative
    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia <t>atrofusca</t> <t>NRRL</t> 22316 and G. cicatricum <t>CBS</t> 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.
    Derivative, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/derivative/product/ATCC
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    derivative - by Bioz Stars, 2024-02
    91/100 stars
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    Image Search Results


    Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia atrofusca NRRL 22316 and G. cicatricum CBS 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.

    Journal: Studies in Mycology

    Article Title: Fusarium : more than a node or a foot-shaped basal cell

    doi: 10.1016/j.simyco.2021.100116

    Figure Lengend Snippet: Maximum-Likelihood (IQ-TREE-ML) consensus tree inferred from the combined acl1 , CaM , ITS, LSU, rpb1 , rpb2 , and tef1 sequence alignment of members of the genus Neocosmospora . Numbers at the branches indicate support values (RAxML-BS / UFboot2-BS / I-PP) above 70 % / 0.95 with thickened branches indicating full support (RAxML-BS / UFboot2-BS = 100 %; BI-PP = 1). Novel taxa are indicated in bold . The scale bar indicates expected changes per site. The tree is rooted to Geejayessia atrofusca NRRL 22316 and G. cicatricum CBS 125552. Ex-epitype, ex-neotype, ex-paratype and ex-type strains are indicated with ET, NT, PT, and T, respectively.

    Article Snippet: Ex-type culture : ATCC 42771 = CBS 130185 = IMI 300797 = NRRL 20423.

    Techniques: Sequencing