Journal: Biomedicines

Article Title: Anti-Microbial Activity of Phytocannabinoids and Endocannabinoids in the Light of Their Physiological and Pathophysiological Roles

doi: 10.3390/biomedicines10030631

Figure Lengend Snippet: Examples of Cannabis sativa constituents that have been documented to possess anti-bacterial, anti-fungal, and/or anti-protozoal activities *.

Article Snippet: 4-Acetoxy-2-geranyl-5-hydroxy-3-n-pentylphenol , IC 50 : 6.7 μM against MRSA ATCC 33591 IC 50 : 12.2 μM against Staphylococcus aureus ATCC 29213 IC 50 : 53.4 μM against Candida krusei ATCC 6258 IC 50 : 42.7 μM against Leishmania donovani Not active against Escherichia coli, Mycobacterium intracellulare, Candida albicans , or Plasmodium falciparum . , [ ] .

Techniques: Activity Assay, Over Expression

Journal: 3 Biotech

Article Title: Draft genome analysis of the endophyte, Bacillus toyonensis COPE52, a blueberry ( Vaccinium spp. var. Biloxi) growth-promoting bacterium

doi: 10.1007/s13205-019-1911-5

Figure Lengend Snippet: ANI and GGDC values obtained from the comparison of strain COPE52 and closely related Bacilli species (16S rRNA > 98.7%) and genome-to genome distance calculator (> 70%)

Article Snippet: Based on pre-stablished cut-off values on species delimitation for ANI > 95–96% (Varghese et al. 2015 ) and GGDC > 70% (Espariz et al. 2016 ), COPE52 was found to be affiliated to Bacillus toyonensis (Table ). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacillus toyonensis COPE52 vs Ortho ANI (%) GGDC (%) Bacillus toyonensis BCT-7112 T 98.55 87.4 Bacillus thuringiensis ATCC 10792 T 91.48 44.6 Bacillus cereus ATCC 14579 T 91.41 44.3 Bacillus wiedmannii FSL W8-0169 T 91.18 43.3 Bacillus luti TD41 T 90.95 43 Bacillus tropicus N24 90.93 42.7 Bacillus mobilis AH1271 90.92 42.6 Bacillus paranthracis Mn5 90.83 42.2 Bacillus anthracis Ames 90.81 42.5 Bacillus albus N35-10-2 90.8 42.3 Bacillus pacificus EB422 90.72 41.9 Bacillus proteolyticus TD42 90.61 40.1 Bacillus nitratireducens 4049 90.41 41.4 Bacillus mycoides ATCC 6462 90.23 40.9 Bacillus paramycoides NH24A2 89.65 38.9 Bacillus gaemokensis KCTC 13318 82.84 26.6 Bacillus bingmayongensis FJAT-13831 82.81 27.3 Bacillus pseudomycoides DSM 12442 82.54 27.2 Open in a separate window ANI and GGDC values obtained from the comparison of strain COPE52 and closely related Bacilli species (16S rRNA > 98.7%) and genome-to genome distance calculator (> 70%) Phylogenomic analysis of B. toyonensis and other Bacilli also corroborated a close relationship within a single clade with B. toyonensis BCT-7112 T , B. toyonensis Rock1-3, and B. toyonensis Rock3-28, which were grouped apart from B. cereus ATCC 14579 T , B. cereus B4264, B. thuringiensis ATCC 10792 T , B. thuringiensis BMB171, B. luti AFS058404, B. luti TD41 T , B. wiedmannii BAG30-1, and B. wiedmannii FSL W8-0169 T . Genomic sequences were aligned by the reference sequence alignment-based phylogeny builder method, REALPHY (Bertels et al. 2014 ,) and the genome-based phylogenetic tree was constructed by MEGA 7 (Kumar et al. 2016 ), using the Neighbor-Joining method, with a bootstrap support of 1000 replications (Fig. ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 1 caption a7 Phylogenetic relationships among strain COPE52 and its closely related Bacillus species: B. toyonensis BCT-7112 T (GCA_000496285.1), B. toyonensis Rock1-3 (GCA_000161155.1), B. toyonensis Rock3-28 (GCA_000161195.1), B. cereus ATCC 14579 T (GCA_000007825.1), B. cereus B4264 (GCA_000021205.1), B. thuringiensis ATCC 10792 T (GCA_000161615.1), B. thuringiensis BMB171 (GCA_000092165.1), B. luti AFS058404 (GCA_002571085.1), B. luti TD41 T (GCA_001884105.1), B. wiedmannii BAG30-1 (GCA_000399645.1) and B. wiedmannii FSL W8-0169 T (GCA_001583695.1).

Techniques: