½ mic  (ATCC)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Effect of endocrine disruptors on bacterial virulence

doi: 10.3389/fcimb.2023.1292233

Figure Lengend Snippet: Overview of the effects of endocrine disruptors on bacterial virulence reported in the literature.

Article Snippet: , Streptococcus mutans ATCC 25175 Streptococcus mutans ATCC 35668 , Exposure to TCS at ½ MIC, ¼ MIC during biofilm formation Adhesion assay on human gingival epithelial cell line OBA-9 of S. mutans cultures exposed to TCS (1/2, 1/4 or 1/8 MIC) Gene expression atlA , gtfC , gtfB comD luxS , assessed by RT-qPCR after 2h exposure to TCS (1/2, 1/4 or 1/8 MIC) , • TCS increased biofilm formation in S. mutans • At ½ MIC and ¼ MIC TCS, the biofilm is thicker with aggregates and microcolonies • At ½ MIC (42.5%) and ¼ MIC TCS, adhesion of ATCC 25175 is enhanced on OBA-9. No significative effect on cell surface hydrophobicity • Increased expression of gene involved adhesion and biofilm at TCS sub inhibitory concentrations , ( ) .

Techniques: Bacteria, Membrane, Permeability, Expressing, Infection, In Vivo, Cell Adhesion Assay, Cell Surface Hydrophobicity, Plasmid Preparation, Transformation Assay, Concentration Assay, Mutagenesis, In Vitro, Mouse Assay, Control, Activity Assay, Staining, Cytometry, Metabolic Assay

Journal: International Journal of Molecular Sciences

Article Title: Hypoxia, but Not Normoxia, Reduces Effects of Resveratrol on Cisplatin Treatment in A2780 Ovarian Cancer Cells: A Challenge for Resveratrol Use in Anticancer Adjuvant Cisplatin Therapy

doi: 10.3390/ijms24065715

Figure Lengend Snippet: Analysis of cisplatin, resveratrol, and in combination to induce apoptosis in OC cells under normoxic and hypoxic conditions by flow cytometry and Western blot. A2780 cells were treated with cisplatin (CisPt) (5.9 µM), resveratrol (Res) (63 µM), and in the combination CisPt+Res (at the same concentrations) according to : Establishment of Hypoxic Culture Conditions and Experimental Outline. ( A ) Percentage of apoptotic cells; ( B ) percentage of necrotic cells; ( C ) representative histograms from apoptosis analysis in A2780 cells; ( D – G ) Western blot analysis of cleaved caspase-3, cleaved caspase-7, and BAX protein expression. The graphs represent the densitometric analysis of the immunoblots. Vinculin was used as a loading control and normalized to the normoxia control as 1. The results shown are the mean ± SEM of three independent experiments, with *** p < 0.001, ** p < 0.01, * p < 0.05, vs. normoxia control (black asterisk) and vs. hypoxia control (grey asterisk); $$$ p < 0.001, $$ p < 0.01, $ p < 0.05 comparing the normoxia group with the hypoxia group; and ### p < 0.001, CisPt alone vs. CisPt+Res.

Article Snippet: After blocking, membranes were incubated overnight at 4 °C with: anti-cleaved caspase-3 (5A1E), anti-cleaved caspase-7 (D6H1), anti-caspase-3 (8G10), anti-caspase-7 (D2Q3L), anti-BAX (D2E11), anti-β-catenin (D10A8), anti-ZEB1 (D80D3), anti-vimentin (D21H3), anti-ZO-1 (all from Cell Signaling Technology, Danvers, MA, USA; isotype IgG), anti-HIF-1α (28b), anti-vinculin (V284), anti-SNAI1 (G-7), and anti-SNAI2 (A-7) antibodies (from Santa Cruz Biotechnology, Dallas, TX, USA; isotype IgG 1 kappa), followed by incubation with horse anti-mouse or goat anti-rabbit IgG secondary antibodies conjugated with horseradish peroxidase (1:10.000; Vector Laboratories, Burlingame, CA, USA) for 1 h at RT.

Techniques: Flow Cytometry, Western Blot, Expressing

Journal: Foods

Article Title: The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review

doi: 10.3390/foods12020415

Figure Lengend Snippet: Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.

Article Snippet: Thailand ( A. mellifera ) , -Cardanol -Cardol , Model: Gastric carcinoma KATO-III (ATCC No. HTB 103) cell line. Protocol: In vitro cytotoxic activity was assessed by the MTT method. A non-transformed human foreskin fibroblast cell line (Hs27, ATCC No. CRL 1634) was used for the comparison. Chemical analysis of the fractions by NMR and ESI-MS. , -Hexane extract IC 50 of 42.5 ± 6.61 μg/mL. -Dichloromethane extract IC 50 of 43.8 ± 6.5 μg/mL. -Fractions obtained from the hexane extract with cytotoxic activity: Fraction III IC 50 of 13.69 ± 1.44 μg/mL. Fraction IV IC 50 of 40.16 ± 2.66 μg/mL. Fraction V IC 50 of 15.21 ± 2.13 μg/mL. -Compounds identified in fractions III and V obtained from hexane extract: Cardanol (fraction III) IC 50 of 13.71 ± 1.42 μg/mL. Cardol (fraction V) IC 50 of 8.78 ± 0.28 μg/mL. , [ ] .

Techniques: In Vitro, In Vivo, Activity Assay, Antioxidant Assay, Positive Control, Antioxidant Activity Assay, CCK-8 Assay, Permeability, Flow Cytometry, Western Blot, Microscopy, Activation Assay, Expressing, Staining, Immunohistochemistry, Cell Culture, Concentration Assay, TUNEL Assay, Mouse Assay

Journal: Acta Ophthalmologica

Article Title: 12‐month outcomes of ranibizumab versus aflibercept for macular oedema in central retinal vein occlusion: data from the FRB! registry

doi: 10.1111/aos.15014

Figure Lengend Snippet: Demographic characteristics of all treatment‐naïve CRVO eyes commencing ranibizumab or aflibercept treatment 2014–2019.

Article Snippet: Baseline VA (SD) was somewhat better in aflibercept‐ versus ranibizumab‐treated eyes (42.5 ± 25.5 letters versus 36.9 ± 26 letters; p = 0.07) with similar CST (614 (240) μm versus 616 (234) μm: p = 0.95).

Techniques:

Journal: Acta Ophthalmologica

Article Title: 12‐month outcomes of ranibizumab versus aflibercept for macular oedema in central retinal vein occlusion: data from the FRB! registry

doi: 10.1111/aos.15014

Figure Lengend Snippet: 12‐month outcomes in all eyes and stratified by anti‐VEGF agent received. Significant p‐values comparing ranibizumab and aflibercept are highlighted in bold.

Article Snippet: Baseline VA (SD) was somewhat better in aflibercept‐ versus ranibizumab‐treated eyes (42.5 ± 25.5 letters versus 36.9 ± 26 letters; p = 0.07) with similar CST (614 (240) μm versus 616 (234) μm: p = 0.95).

Techniques: