Journal: Medical mycology

Article Title: Paradoxical antifungal activity and structural observations in biofilms formed by echinocandin-resistant Candida albicans clinical isolates

doi: 10.1093/mmy/myt007

Figure Lengend Snippet: . Comparison of echinocandin activity on planktonic (pMIC 80 ) and sessile (sMIC 80 ) cells of Candida albicans fks1 mutants.

Article Snippet: Two fks1 mutant strains (32746 and 5415) were categorized as poor biofilm producers, four (2762, 4254, 4715, and 42286) and the three ATCC reference strains were moderate biofilm producers, and six fks1 mutant strains (41509, 42379, 42996, 43001, 53264, and 41301) were categorized as strong biofilm producers.

Techniques: Activity Assay, Mutagenesis

Journal: Medical mycology

Article Title: Paradoxical antifungal activity and structural observations in biofilms formed by echinocandin-resistant Candida albicans clinical isolates

doi: 10.1093/mmy/myt007

Figure Lengend Snippet: . Comparison of paradoxical effect concentrations of planktonic and sessile cells of Candida albicans fks1 mutants.

Article Snippet: Two fks1 mutant strains (32746 and 5415) were categorized as poor biofilm producers, four (2762, 4254, 4715, and 42286) and the three ATCC reference strains were moderate biofilm producers, and six fks1 mutant strains (41509, 42379, 42996, 43001, 53264, and 41301) were categorized as strong biofilm producers.

Techniques: Mutagenesis

Journal: Medical mycology

Article Title: Paradoxical antifungal activity and structural observations in biofilms formed by echinocandin-resistant Candida albicans clinical isolates

doi: 10.1093/mmy/myt007

Figure Lengend Snippet: Assessment of biofilm mass of Candida albicans reference strains and fks1 clinical isolates. Biofilms were grown in triplicate at a concentration of 1 × 106 cells/ml in buffered RPMI-1640 at 37°C for 24 h. Biofilm mass was quantified using the crystal violet assay. Light absorbance was measured in a plate reader at OD 630 nm. Each experiment was performed independently three times.

Article Snippet: Two fks1 mutant strains (32746 and 5415) were categorized as poor biofilm producers, four (2762, 4254, 4715, and 42286) and the three ATCC reference strains were moderate biofilm producers, and six fks1 mutant strains (41509, 42379, 42996, 43001, 53264, and 41301) were categorized as strong biofilm producers.

Techniques: Concentration Assay, Crystal Violet Assay

Journal: Medical mycology

Article Title: Paradoxical antifungal activity and structural observations in biofilms formed by echinocandin-resistant Candida albicans clinical isolates

doi: 10.1093/mmy/myt007

Figure Lengend Snippet: Assessment of biofilm metabolic activity of Candida albicans reference strains and fks1 clinical isolates. Biofilms were grown in quadruplicate at a concentration of 1 × 106 cells/ml in buffered RPMI-1640 at 37° C for 24 h. The XTT assay was used to assay sessile metabolic activity. Formation of the colored formazan was subsequently measured at OD 490 nm. Each experiment was performed independently three times.

Article Snippet: Two fks1 mutant strains (32746 and 5415) were categorized as poor biofilm producers, four (2762, 4254, 4715, and 42286) and the three ATCC reference strains were moderate biofilm producers, and six fks1 mutant strains (41509, 42379, 42996, 43001, 53264, and 41301) were categorized as strong biofilm producers.

Techniques: Activity Assay, Concentration Assay, XTT Assay

Journal: Medical mycology

Article Title: Paradoxical antifungal activity and structural observations in biofilms formed by echinocandin-resistant Candida albicans clinical isolates

doi: 10.1093/mmy/myt007

Figure Lengend Snippet: Ultrastructural assessment of biofilm morphology using scanning electron microscopy. (A) Scanning electron microscopy of representative Candida albicans fks1 mutants that form poor (4254), moderate (42286), and strong (53264) biofilms compared with reference strain SC5314. (B) Scanning electron microscopy view of pit-like cell surface structures identified on select C. albicans fks1 mutants.

Article Snippet: Two fks1 mutant strains (32746 and 5415) were categorized as poor biofilm producers, four (2762, 4254, 4715, and 42286) and the three ATCC reference strains were moderate biofilm producers, and six fks1 mutant strains (41509, 42379, 42996, 43001, 53264, and 41301) were categorized as strong biofilm producers.

Techniques: Electron Microscopy

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Cranberry-derived proanthocyanidins prevent formation of Candida albicans biofilms in artificial urine through biofilm- and adherence-specific mechanisms

doi: 10.1093/jac/dkt398

Figure Lengend Snippet: Effect of cranberry PACs on C. albicans biofilm formation

Article Snippet: 33 – 35 Notably, the MIC 80 for all four urinary isolates and for one of two caspofungin-resistant fks1 mutants, 42379, was appreciably elevated compared with other strains (>1024 mg/L). table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Strain MIC­ 50 (mg/L) MIC 80 (mg/L) SC5314 16 128 ATCC 10231 8 64 ATCC 14053 128 512 ATCC 24433 64 512 42379 ( fks1 mutant) 1024 >1024 53264 ( fks1 mutant) 64 256 C. albicans urinary isolate 3 16 >1024 C. albicans urinary isolate 5 32 >1024 C. albicans urinary isolate 6 >1024 >1024 C. albicans urinary isolate 8 >1024 >1024 Open in a separate window Effect of cranberry PACs on C. albicans biofilm formation The combination of cranberry PACs with fluconazole, amphotericin B and caspofungin against biofilm formation had an additive, but not synergistic, effect (data not shown).

Techniques: Mutagenesis