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Effects of SCH58261 on protein phosphorylation and expression. A , the levels of phospho-protein bands were quantified and normalized to the levels of the total proteins detected in the same samples. B , p47 phox was immunoprecipitated down and detected for serine phosphorylation and binding to Nox2. C , two-way immunoprecipitation for the detection of AngII-induced Nox2 association with A <t>2A</t> <t>R</t> is shown. *, p < 0.05 for AngII values versus vehicle controls. †, p < 0.05 for SCH58261values versus values without SCH58261 in the same treatment group. Error bars , S.D.
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Effects of SCH58261 on protein phosphorylation and expression. A , the levels of phospho-protein bands were quantified and normalized to the levels of the total proteins detected in the same samples. B , p47 phox was immunoprecipitated down and detected for serine phosphorylation and binding to Nox2. C , two-way immunoprecipitation for the detection of AngII-induced Nox2 association with A 2A R is shown. *, p < 0.05 for AngII values versus vehicle controls. †, p < 0.05 for SCH58261values versus values without SCH58261 in the same treatment group. Error bars , S.D.

Journal: The Journal of Biological Chemistry

Article Title: Inactivation of Adenosine A 2A Receptor Attenuates Basal and Angiotensin II-induced ROS Production by Nox2 in Endothelial Cells

doi: 10.1074/jbc.M110.184606

Figure Lengend Snippet: Effects of SCH58261 on protein phosphorylation and expression. A , the levels of phospho-protein bands were quantified and normalized to the levels of the total proteins detected in the same samples. B , p47 phox was immunoprecipitated down and detected for serine phosphorylation and binding to Nox2. C , two-way immunoprecipitation for the detection of AngII-induced Nox2 association with A 2A R is shown. *, p < 0.05 for AngII values versus vehicle controls. †, p < 0.05 for SCH58261values versus values without SCH58261 in the same treatment group. Error bars , S.D.

Article Snippet: The control siRNA and A 2A R siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Immunoprecipitation, Binding Assay

Effects of A 2A R siRNA on Nox2 expression and activity. A , protein bands were quantified and normalized to the levels of α-tubulin detected in the same samples. B , NADPH-dependent O 2 ˙̄ production detected by lucigenin chemilunescence. MLU , mean light unit. *, p < 0.05 for indicated values versus vehicle control siRNA values. †, p < 0.05 for indicated values versus control siRNA values in the same treatment group. Error bars , S.D.

Journal: The Journal of Biological Chemistry

Article Title: Inactivation of Adenosine A 2A Receptor Attenuates Basal and Angiotensin II-induced ROS Production by Nox2 in Endothelial Cells

doi: 10.1074/jbc.M110.184606

Figure Lengend Snippet: Effects of A 2A R siRNA on Nox2 expression and activity. A , protein bands were quantified and normalized to the levels of α-tubulin detected in the same samples. B , NADPH-dependent O 2 ˙̄ production detected by lucigenin chemilunescence. MLU , mean light unit. *, p < 0.05 for indicated values versus vehicle control siRNA values. †, p < 0.05 for indicated values versus control siRNA values in the same treatment group. Error bars , S.D.

Article Snippet: The control siRNA and A 2A R siRNA were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Activity Assay

Effects of A 2A R siRNA on MAPK and Akt phosphorylation. The phospho-protein bands were quantified and normalized to the total levels of the same proteins detected in the same samples. *, p < 0.05 for AngII values versus values without AngII in the same group. †, p < 0.05 for indicated values versus AngII values in control siRNA group. Error bars , S.D.

Journal: The Journal of Biological Chemistry

Article Title: Inactivation of Adenosine A 2A Receptor Attenuates Basal and Angiotensin II-induced ROS Production by Nox2 in Endothelial Cells

doi: 10.1074/jbc.M110.184606

Figure Lengend Snippet: Effects of A 2A R siRNA on MAPK and Akt phosphorylation. The phospho-protein bands were quantified and normalized to the total levels of the same proteins detected in the same samples. *, p < 0.05 for AngII values versus values without AngII in the same group. †, p < 0.05 for indicated values versus AngII values in control siRNA group. Error bars , S.D.

Article Snippet: The control siRNA and A 2A R siRNA were purchased from Santa Cruz Biotechnology.

Techniques:

Effect of SCH58261 or A 2A R KO on vessel relaxation. A , endothelium-dependent relaxation to acetylcholine of WT aortic rings. *, p < 0.05 for AngII values versus vehicle controls ( left panel ) or versus the values in the presence of SCH58261 or tiron ( right panel ). B , endothelium-independent relaxation to sodium nitroprusside ( SNP )of WT aortic rings. C , NADPH-dependent ROS production detected by lucigenin chemiluminescence. MLU , mean light unit. *, p < 0.05 for AngII versus vehicle controls in WT. †, p < 0.05 for A 2A R KO versus WT treated with AngII. D , endothelium-dependent relaxation to acetylcholine ( ACh ) of A 2A R KO aortic rings. Error bars , S.D.

Journal: The Journal of Biological Chemistry

Article Title: Inactivation of Adenosine A 2A Receptor Attenuates Basal and Angiotensin II-induced ROS Production by Nox2 in Endothelial Cells

doi: 10.1074/jbc.M110.184606

Figure Lengend Snippet: Effect of SCH58261 or A 2A R KO on vessel relaxation. A , endothelium-dependent relaxation to acetylcholine of WT aortic rings. *, p < 0.05 for AngII values versus vehicle controls ( left panel ) or versus the values in the presence of SCH58261 or tiron ( right panel ). B , endothelium-independent relaxation to sodium nitroprusside ( SNP )of WT aortic rings. C , NADPH-dependent ROS production detected by lucigenin chemiluminescence. MLU , mean light unit. *, p < 0.05 for AngII versus vehicle controls in WT. †, p < 0.05 for A 2A R KO versus WT treated with AngII. D , endothelium-dependent relaxation to acetylcholine ( ACh ) of A 2A R KO aortic rings. Error bars , S.D.

Article Snippet: The control siRNA and A 2A R siRNA were purchased from Santa Cruz Biotechnology.

Techniques: