Journal: Journal of Histochemistry and Cytochemistry

Article Title: Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors

doi: 10.1369/0022155417725304

Figure Lengend Snippet: Summary of α7 nAChR (α7) Antibodies Used.

Article Snippet: Lot No. Reported Species Reactivity Reported Epitope Target on α7 nAChR Sequence Western Blot/Immunofluorescence Data Monoclonal or Polyclonal Secondary Antibody Target # Citations (CiteAb) # Rat Citations (CiteAb) Abcam ab23832 a GR89867-1 h, r, m 1–100 α7 Amino acid sequence −/− Polyclonal Rabbit 17 4 EMD Millipore AB15332 2630253 m 300–400 α7 Amino acid sequence +/+ b Polyclonal Rabbit 0 0 EMD Millipore MABN529 Q2459834 h, m Cytoplasmic domain −/− Monoclonal Mouse 0 0 Novus Biologicals NBP1-79948 QC0391-42133 h, r, m N-terminal domain +/++ Polyclonal Rabbit 0 0 Novus Biologicals NBP1-79948 QC42753-42341 h, r, m N-terminal domain −/NA Polyclonal Rabbit 0 0 Santa Cruz Biotech. sc-5544 a (H-302) D2214 h, r, m 367–502 α7 Amino acid sequence −/− Polyclonal Rabbit 31 6 Santa Cruz Biotech. sc-58607 (MAb 319) a L0312 h, r, m, c Cytoplasmic domain −/− Monoclonal Rat 5 Sigma, 3 Santa Cruz 1 Santa Cruz Sigma-Aldrich M220 (MAb 306) a 040K4705 h, r, c 380–400 α7 Amino acid sequence +/+ Monoclonal MouseTotal citations 1066 415 Open in a separate window Reported species reactivity and epitope locations are taken from the manufacturer’s literature.

Techniques: Sequencing, Western Blot

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors

doi: 10.1369/0022155417725304

Figure Lengend Snippet: α7 nAChR detection using two different lots of NBP1-79948 antibody. (A) Representative images of the western blot and immunofluorescence results for Novus NBP1-79948, rabbit polyclonal α7 nAChR antibody (lot QC0391-42133) at a 1:1000 dilution. Western blot showing immunoreactivity toward both α7- and α7-GFP-transfected GH4C1 cells. Multiple cross-reactive nonspecific bands were found across each lane. Labeling pattern between untransfected and α7 nAChR-transfected GH4C1 cells looks similar at a 1:100 dilution. 1:1000 dilution of the same antibody from the same lot showed significant differences between WT GH4C1 cells and cells transfected with α7 nAChR. (B) Immunoblotting results obtained with lot QC42753-42341 of NBP1-79948 α7 nAChR antibody did not show significant differences between untransfected and α7 nAChR-transfected GH4C1 cell extracts. Scale bar, 40 μm. Abbreviations: nAChR, nicotinic acetylcholine receptors; GFP, green fluorescent protein; WT, wild-type.

Article Snippet: Lot No. Reported Species Reactivity Reported Epitope Target on α7 nAChR Sequence Western Blot/Immunofluorescence Data Monoclonal or Polyclonal Secondary Antibody Target # Citations (CiteAb) # Rat Citations (CiteAb) Abcam ab23832 a GR89867-1 h, r, m 1–100 α7 Amino acid sequence −/− Polyclonal Rabbit 17 4 EMD Millipore AB15332 2630253 m 300–400 α7 Amino acid sequence +/+ b Polyclonal Rabbit 0 0 EMD Millipore MABN529 Q2459834 h, m Cytoplasmic domain −/− Monoclonal Mouse 0 0 Novus Biologicals NBP1-79948 QC0391-42133 h, r, m N-terminal domain +/++ Polyclonal Rabbit 0 0 Novus Biologicals NBP1-79948 QC42753-42341 h, r, m N-terminal domain −/NA Polyclonal Rabbit 0 0 Santa Cruz Biotech. sc-5544 a (H-302) D2214 h, r, m 367–502 α7 Amino acid sequence −/− Polyclonal Rabbit 31 6 Santa Cruz Biotech. sc-58607 (MAb 319) a L0312 h, r, m, c Cytoplasmic domain −/− Monoclonal Rat 5 Sigma, 3 Santa Cruz 1 Santa Cruz Sigma-Aldrich M220 (MAb 306) a 040K4705 h, r, c 380–400 α7 Amino acid sequence +/+ Monoclonal MouseTotal citations 1066 415 Open in a separate window Reported species reactivity and epitope locations are taken from the manufacturer’s literature.

Techniques: Western Blot, Immunofluorescence, Transfection, Labeling

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors

doi: 10.1369/0022155417725304

Figure Lengend Snippet: Summary of α7 nAChR (α7) Antibodies Used.

Article Snippet: Novus Biologicals , NBP1-79948 , QC0391-42133 , h, r, m , N-terminal domain , +/++ , Polyclonal , Rabbit , 0 , 0.

Techniques: Sequencing, Western Blot

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors

doi: 10.1369/0022155417725304

Figure Lengend Snippet: α7 nAChR detection using two different lots of NBP1-79948 antibody. (A) Representative images of the western blot and immunofluorescence results for Novus NBP1-79948, rabbit polyclonal α7 nAChR antibody (lot QC0391-42133) at a 1:1000 dilution. Western blot showing immunoreactivity toward both α7- and α7-GFP-transfected GH4C1 cells. Multiple cross-reactive nonspecific bands were found across each lane. Labeling pattern between untransfected and α7 nAChR-transfected GH4C1 cells looks similar at a 1:100 dilution. 1:1000 dilution of the same antibody from the same lot showed significant differences between WT GH4C1 cells and cells transfected with α7 nAChR. (B) Immunoblotting results obtained with lot QC42753-42341 of NBP1-79948 α7 nAChR antibody did not show significant differences between untransfected and α7 nAChR-transfected GH4C1 cell extracts. Scale bar, 40 μm. Abbreviations: nAChR, nicotinic acetylcholine receptors; GFP, green fluorescent protein; WT, wild-type.

Article Snippet: Novus Biologicals , NBP1-79948 , QC0391-42133 , h, r, m , N-terminal domain , +/++ , Polyclonal , Rabbit , 0 , 0.

Techniques: Western Blot, Immunofluorescence, Transfection, Labeling