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atgl nb110-41536 antibody  (Novus Biologicals)
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A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold <t>change</t> <t>abundance</t> of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H <t>ATGL</t> protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )
Atgl Nb110 41536 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p p65  (Santa Cruz Biotechnology)
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A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold <t>change</t> <t>abundance</t> of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H <t>ATGL</t> protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )
P P65, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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national collections of industrial, marine and food bacteria (ncimb) deposit accession number 41536  (NCIMB Ltd)
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NCIMB Ltd national collections of industrial, marine and food bacteria (ncimb) deposit accession number 41536
A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold <t>change</t> <t>abundance</t> of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H <t>ATGL</t> protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )
National Collections Of Industrial, Marine And Food Bacteria (Ncimb) Deposit Accession Number 41536, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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accession no. 41536  (NCIMB Ltd)
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NCIMB Ltd accession no. 41536
A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold <t>change</t> <t>abundance</t> of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H <t>ATGL</t> protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )
Accession No. 41536, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold change abundance of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H ATGL protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )

Journal: Cellular and Molecular Immunology

Article Title: Fatty acid desaturation by stearoyl-CoA desaturase-1 controls regulatory T cell differentiation and autoimmunity

doi: 10.1038/s41423-023-01011-2

Figure Lengend Snippet: A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold change abundance of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H ATGL protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )

Article Snippet: For analysis of ATGL abundance, the following antibody was used: ATGL (NB110-41536, 1:100; Novus, Belgium, Brugge).

Techniques: Mass Spectrometry, Expressing, Two Tailed Test