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gsk690693  (Tocris)
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Tocris gsk690693
Gsk690693, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dynasylan 4144  (Evonik Degussa GmbH)
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Dynasylan 4144, supplied by Evonik Degussa GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt inhibitor gsk690693  (Tocris)
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Tocris akt inhibitor gsk690693
Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, <t>GSK690693;</t> LY, LY294002.
Akt Inhibitor Gsk690693, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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heavy isotope internal standards  (Cambridge Isotope Laboratories)
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Cambridge Isotope Laboratories heavy isotope internal standards
Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, <t>GSK690693;</t> LY, LY294002.
Heavy Isotope Internal Standards, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b1 4144 b  (CPG Inc)
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CPG Inc b1 4144 b
Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, <t>GSK690693;</t> LY, LY294002.
B1 4144 B, supplied by CPG Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b1 4144 b - by Bioz Stars, 2025-03
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gsk 690693 4144 standard  (Tocris)
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Tocris gsk 690693 4144 standard
Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, <t>GSK690693;</t> LY, LY294002.
Gsk 690693 4144 Standard, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fabrikmattenweg 4 ch 4144  (Lipomed)
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Lipomed fabrikmattenweg 4 ch 4144
Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, <t>GSK690693;</t> LY, LY294002.
Fabrikmattenweg 4 Ch 4144, supplied by Lipomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fabrikmattenweg 4 ch 4144/product/Lipomed
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fabrikmattenweg 4 ch 4144 - by Bioz Stars, 2025-03
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isom 4144  (NCIMB Ltd)
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NCIMB Ltd isom 4144
Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, <t>GSK690693;</t> LY, LY294002.
Isom 4144, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isom 4144 - by Bioz Stars, 2025-03
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Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, GSK690693; LY, LY294002.

Journal: Endocrinology

Article Title: Nongenomic ERα-AMPK Signaling Regulates Sex-Dependent Bcrp Transport Activity at the Blood-Brain Barrier

doi: 10.1210/endocr/bqae081

Figure Lengend Snippet: Signaling downstream of ERα involves AMPK. (A) Inhibition Akt and PI3K does not block E2-elicited decrease in specific Bcrp transport activity. (B) Treatment with an inhibitor of AMPK completely attenuates E2-elicited decrease in specific Bcrp transport activity. (C) Western blots of MBC cytosolic fractions showing increase in phosphorylated AMPKβ1 following exposure of MBCs to 1 nM E2 for 30 minutes. (D) Abbreviated working model. Each bar or point represents the mean value for 10 to 18 capillaries from a single isolation with tissue pooled from 5 mice. SEM bars depict variability and units are arbitrary fluorescence. Statistical comparisons: ***P < .001. Abbreviations: Bcrp, breast cancer resistance protein; DMD, dorsomorphin dihydrochloride; E2, 17β-estradiol; ERα, estrogen receptor α; ERαKO; GSK, GSK690693; LY, LY294002.

Article Snippet: The Bcrp-specific inhibitor KO143, P-glycoprotein-specific inhibitor PSC833, Multidrug resistance-associated protein 2 (Mrp2)-specific inhibitor MK571, ERα agonist propyl pyrazole triol (PPT), ERβ agonist diarylpropionitrile (DPN), GPER agonist G1, Akt inhibitor GSK690693, PI3K inhibitor LY294002, and AMPK inhibitor dorsomorphin dihydrochloride were all purchased from Tocris Bioscience.

Techniques: Inhibition, Blocking Assay, Activity Assay, Western Blot, Isolation, Fluorescence