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Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total <t>cholesterol,</t> FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.
Free Cholesterol Kit Spinreact 41035, supplied by SPINREACT SA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total <t>cholesterol,</t> FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.
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Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total <t>cholesterol,</t> FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.
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Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total <t>cholesterol,</t> FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.
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Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total <t>cholesterol,</t> FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.
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Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total cholesterol, FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.

Journal: Scientific Reports

Article Title: Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

doi: 10.1038/s41598-020-72777-y

Figure Lengend Snippet: Body weight, fat mass and lean mass gain during eIMF or cIMF feeding. Plasma lipids, adipokines, cytokines and bile acids at PN42. Body weight ( A ), fat and lean mass ( B ) are expressed in absolute weights. Plasma lipids at PN21 (weaning) and at PN42 ( C ). All other parameters were measured at PN42. Liver, epididymal (epi) and perirenal (peri) fat pads weights obtained at dissection at PN42 are expressed absolutely ( D ). Plasma adipokines ( E , F ), glucostatic hormones ( G ) and cytokines ( H , all 4 h fasting at PN42) are expressed as absolute concentration. Plasma bile acid species are expressed as a percentage ( I , PN42). TG triglycerides, TC total cholesterol, FC free cholesterol, CE cholesterol ester, NEFA non-esterified fatty acids, Phos phospholipids, MCP-1 monocyte chemoattractant protein-1, TNFα tumor necrosis factor alpha, IL-1b/2/5/6/10 interleukin 1b/2/5/6/10, IFNg interferon gamma, CXCL-1 CXC chemokine ligand 1, (T-) (L) CA (tauro-) (litho) cholic acid, (T/G-) (U/C/H) DCA (tauro-) (urso/cheno/hyo) deoxycholic acid, (T)A/B-MCA (tauro-) α/β-muricholic acid. ( A – I ) n = 12–16; ( A , B ) Median ± interquartile range. ( C – I ) Tukey boxplots and scatter plots. Exact two-sided Mann–Whitney U test **p < 0.01, *p < 0.05.

Article Snippet: Plasma was analyzed using commercially available kits for triglycerides (Roche, 11877771216), total cholesterol (Roche, 11491458216), free cholesterol (Spinreact, 41035), NEFA (Sopachem, 157819910935), and phospholipids (Sopachem, 157419910930).

Techniques: Clinical Proteomics, Dissection, Concentration Assay, MANN-WHITNEY

Calculated nutrient composition of the diets.

Journal: Scientific Reports

Article Title: Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

doi: 10.1038/s41598-020-72777-y

Figure Lengend Snippet: Calculated nutrient composition of the diets.

Article Snippet: Plasma was analyzed using commercially available kits for triglycerides (Roche, 11877771216), total cholesterol (Roche, 11491458216), free cholesterol (Spinreact, 41035), NEFA (Sopachem, 157819910935), and phospholipids (Sopachem, 157419910930).

Techniques: Maltodextrin, Starch