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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: More P450s Are Involved in Secondary Metabolite Biosynthesis in Streptomyces Compared to Bacillus , Cyanobacteria , and Mycobacterium
doi: 10.3390/ijms21134814
Figure Lengend Snippet: Genome-wide data mining and annotation of P450s in 203 Streptomyces species.
Article Snippet:
Techniques: Genome Wide
Journal: Oncogene
Article Title: GATA3 Targets Semaphorin 3B in Mammary Epithelial Cells to Suppress Breast Cancer Progression and Metastasis
doi: 10.1038/onc.2017.165
Figure Lengend Snippet: In silico analysis of breast cancer databases. (a) Analysis of TCGA database. Heat map indicating gene expression analysis for GATA3 , SEMA3B and MYC in multiple breast cancer samples. (N = 597) (b) Analysis of TCGA database via Regulome explorer. Data present a collection of proteins including GATA3 that may reside in the same protein network as SEMA3B. (c) Analysis of SEMA3B expression via the GSE9014 database. SEMA3B expression is significantly lowered in the invasive breast carcinoma samples. (Normal: N = 6, Invasive breast carcinoma: N = 53, P = 1.03E-21) (d) TCGA analysis indicating significantly lowered SEMA3B expression in triple negative breast cancer samples. (Normal: N = 244, Triple negative: N = 49, P = 4.06E-9) (e) Analysis of SEMA3B expression in PAM50 subtypes using the METABRIC database. Luminal breast cancers possess higher levels of SEMA3B expression than the basal subtype, P = <0.0001, Normal: N = 202, Luminal A: N = 721, Luminal B: N = 492. HER2: N = 240, Basal: N = 331) (f) Analysis of breast cancer patient survival . Tumor samples exhibiting lower SEMA3B expression show poorer patient prognosis. (Concordance index = 62.3, Log-rank equal curves P = 1.6E-4, R^ 2 = 0.005/0.941, Risk groups hazard ratio = 2.4, P = 2.61E-6)
Article Snippet:
Techniques: In Silico, Gene Expression, Expressing
Journal: Oncogene
Article Title: GATA3 Targets Semaphorin 3B in Mammary Epithelial Cells to Suppress Breast Cancer Progression and Metastasis
doi: 10.1038/onc.2017.165
Figure Lengend Snippet: GATA3 directly controls SEMA3B expression. (a) ChIP analysis indicating GATA3 transcription factor binding to SEMA3B promoter in EpH4.9, T47D and MDA-MB-231 cells. (b) qPCR analysis of GATA3 and SEMA3B expression in MDA-MB-231 cells, N = 6. Overexpression of GATA3 in MDA-MB-231 cells results in elevation of SEMA3B levels. (c) Western blot analysis of MDA-MB-231 cell lysate. Overexpression of GATA3 enhances SEMA3B expression. (d) Immunostaining of MDA-MB-231 (control) and GATA3 overexpressing MDA-MB-231 cells. Overexpression of GATA3 upregulates SEMA3B levels. Bar 50 μm. (e) qPCR analysis of SEMA3B in NMuMG cells, N = 2. Lowering GATA3 expression via siRNA results in downregulation of SEMA3B levels.
Article Snippet:
Techniques: Expressing, Binding Assay, Over Expression, Western Blot, Immunostaining, Control
Journal: Oncogene
Article Title: GATA3 Targets Semaphorin 3B in Mammary Epithelial Cells to Suppress Breast Cancer Progression and Metastasis
doi: 10.1038/onc.2017.165
Figure Lengend Snippet: Inhibition of cellular migration and proliferation by GATA3 relies on presence of SEMA3B. (a) Bright-field images of MDA-MB-231 stable cell lines in 2D cell cultures. Bar, 50 μm. (b) Phase-contrast images of MDA-MB-231 stable cell lines in 3D cultures. Arrows indicate the presence of invadopodia moving outward from the colony. Bar, 200 μm. (c) Quantification of 3D Matrigel culture colonies. (N = 7) (d) qRT-PCR analysis of EMT-associated genes. RNA samples from MDA-MB-231 control and MDA-SEMA3B cells were collected. qRT-PCR analysis indicates that SEMA3B lowers EMT gene levels. SEMA3B enhanced E-cadherin levels compared to control. (e) Scratch assay analysis indicating difference in cellular migration in MDA-MB-231 stable cell lines. N = 6 (f) Quantification of cell infiltration of gaps between the two invading cell fronts. The gap was measured using ImageJ software. Y-axis indicates experimental group/control group. (N = 6). (g) Colony formation assay. MDA-MB-231 stable cell lines were cultured in 6-well plates to allow colony growth. Colonies were stained with crystal violet and counted. N = 12. (h) Graph representing the quantification of Giemsa stained colonies. (N = 12).
Article Snippet:
Techniques: Inhibition, Migration, Stable Transfection, Quantitative RT-PCR, Control, Wound Healing Assay, Software, Colony Assay, Cell Culture, Staining
Journal: Oncogene
Article Title: GATA3 Targets Semaphorin 3B in Mammary Epithelial Cells to Suppress Breast Cancer Progression and Metastasis
doi: 10.1038/onc.2017.165
Figure Lengend Snippet: Loss of SEMA3B disrupts GATA3 tumor suppressive activity. (a) MDA-SEMA3B and (b) MDA-GATA3-SEMA3B KD stable cell lines where transplanted in 8-week-old female nude mice via orthotopic injection. Color photographs show a representative set of gross tumors from each transplanted group. Graphs indicates tumor measurement during the course of experiment, N = 10 for each group. (c) Immunostaining and quantification analysis for Ki67 expression in the tumor sections (N = 6). Bar, 100 μm. (d) Analysis of inguinal lymph node weight from transplanted mice, N = 10 for each group. (e) H&E analysis of lymph node sections indicating tumor metastasis. For each transplanted group, metastatic tumor cells to the lymph node was quantified (N = 6). Bar = 200 μm. (f) Western blot analysis indicating phosphorylation status of LIMK1 and LIMK2 in control and MDA-SEMA3B cell extracts. Total LIMK1 and LIMK2 indicate equal loading of samples. Protein bands 72 kDa.
Article Snippet:
Techniques: Activity Assay, Stable Transfection, Injection, Immunostaining, Expressing, Western Blot, Phospho-proteomics, Control
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: DR24, DR48 and DR120 denote differentially expressed gene sets obtained from “ROC”22 samples at 24, 48, and 120 h after S. scitamineum inoculation compared to controls 24 h after water inoculation, respectively; DY24, DY48 and DY120 denote differentially expressed gene sets obtained from Yacheng05-179 samples at 24, 48 and 120 h after S. scitamineum inoculation compared to control sample 24 h after water inoculation, respectively. All DEGs: differentially expressed genes.
Article Snippet: Here, we observed that ( )
Techniques: Control
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: DR24, DR48 and DR120 denote differentially expressed gene sets obtained from “ROC”22 samples at 24, 48 and 120 h after S. scitamineum inoculation compared to control sample at 24 h after water inoculation, respectively; DY24, DY48 and DY120 denote differentially expressed gene sets obtained from Yacheng05-179 samples at 24, 48 and 120 h after S. scitamineum inoculation compared to control sample at 24 h after water inoculation, respectively; All DEGs, all differentially expressed genes; Up-regulation DEGs, up-regulated genes; Down-regulation DEGs, down-regulated genes.
Article Snippet: Here, we observed that ( )
Techniques: Control
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: DR-up and DR-down denote continuously up-regulated/down-regulated gene sets in “ROC”22 samples at 24, 48 and 120 h after S. scitamineum inoculation compared to control sample 24 h after water inoculation, respectively; DY-up and DY-down denote continuously up-regulated/down-regulated gene sets in Yacheng05-179 samples at 24, 48 and 120 h after S. scitamineum inoculation compared to control sample 24 h after water inoculation, respectively.
Article Snippet: Here, we observed that ( )
Techniques: Control
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: T1, T2, T3 and T4 denote “ROC”22 at 24 h after water inoculation, and at 24, 48 and 120 h after S. scitamineum inoculation, respectively; T5, T6, T7 and T8 denote Yacheng05-179 at 24 h after water inoculation, and at 24, 48 and 120 h after S. scitamineum inoculation, respectively; k1∼k9 indicate nine distinct expression patterns of differentially co-expressed genes.
Article Snippet: Here, we observed that ( )
Techniques: Expressing
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: T1, T2, T3 and T4 denote “ROC”22 at 24 h after water inoculation, and at 24, 48 and 120 h after S. scitamineum inoculation, respectively.
Article Snippet: Here, we observed that ( )
Techniques:
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: T5, T6, T7 and T8 denote Yacheng05-179 at 24 h after water inoculation, and at 24, 48 and 120 h after S. scitamineum inoculation, respectively.
Article Snippet: Here, we observed that ( )
Techniques:
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: Dynamic expression patterns of differentially expressed genes in “ROC”22 after S. scitamineum inoculation.
Article Snippet: Here, we observed that ( )
Techniques: Expressing
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: Analysis of pathways involving differentially expressed genes after S. scitamineum inoculation in “ROC”22.
Article Snippet: Here, we observed that ( )
Techniques:
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: Analysis of pathways involving differentially expressed genes after S. scitamineum inoculation in Yacheng05-179.
Article Snippet: Here, we observed that ( )
Techniques: Transduction
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: Expression of resistance-related genes in sugarcane after S. scitamineum infection.
Article Snippet: Here, we observed that ( )
Techniques: Expressing, Infection, Transduction
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: (A) Transcript levels of ScChi during sugarcane- Sporisorium scitamineum interaction. The data of RT-qPCR was normalized to the GAPDH expression level. All data points (deduction its mock) are means ±SE (n = 3). Y, Yacheng05-179; R, “ROC”22. 24 h, 48 h and 120 h, sugarcane buds inoculation with S. scitamineum at 24 h, 48 h and 120 h, respectively; qPCR, detection results of real-time fluorescent quantitative PCR; log 2 FC, fold change of the differential expression of chitinase gene in the transcriptome. (B) The infection results of Nicotiana benthamiana Fusarium solani var. coeruleum and Botrytis cinerea by infiltrated with the 35S::ScChi -containing Agrobacterium strain. The disease symptom was assessed 20 d after inoculation. (C) The antimicrobial action of chitinase (T 0 generation of ScC hi transgenic N. benthamiana ) on Fusarium solani var. coeruleum. CK, the control of normal culture on Fusarium solani var. coeruleum ; 35S::ScChi , the antimicrobial action of chitinase of T 0 generation of ScChi transgenic N. benthamiana ; 1∼3, chitinase from three different T 0 generation of ScChi transgenic N. benthamiana , respectively; 4, chitinase from T 0 generation of pCAMBIA 1301 transgenic N. benthamiana ; 5, chitinase from untransgenic N. benthamiana ; 6, 0.1 mol/L sodium acetate buffer (pH 5.0). Read arrow indicated the antibacterial effect.
Article Snippet: Here, we observed that ( )
Techniques: Quantitative RT-PCR, Expressing, Real-time Polymerase Chain Reaction, Quantitative Proteomics, Infection, Transgenic Assay, Control
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: Q1, metacaspase-1-like gene; Q2, ribonuclease 3-like gene; Q3, pathogenesis-related protein (PR-10) gene; Q4, sucrose transporter (SUT1) gene; Q5, vacuolar amino acid transporter 1-like gene; Q6, heat shock protein-like gene. Y, Yacheng05-179; R, “ROC”22; 24 h, 48 h and 120 h, sugarcane buds inoculation with Sporisorium scitamineum at 24 h, 48 h and 120 h, respectively; qPCR, detection results of real-time fluorescent quantitative PCR; log 2 FC, fold change of the differential expression genes in the transcriptome.
Article Snippet: Here, we observed that ( )
Techniques: Real-time Polymerase Chain Reaction, Quantitative Proteomics
Journal: PLoS ONE
Article Title: A Global View of Transcriptome Dynamics during Sporisorium scitamineum Challenge in Sugarcane by RNA-seq
doi: 10.1371/journal.pone.0106476
Figure Lengend Snippet: Dynamic expression patterns of differentially expressed genes in Yacheng05-179 after S. scitamineum inoculation.
Article Snippet: Here, we observed that ( )
Techniques: Expressing