Journal: Biology

Article Title: Post-Irradiation Thymic Regeneration in B6C3F1 Mice Is Age Dependent and Modulated by Activation of the PI3K-AKT-mTOR Pathway

doi: 10.3390/biology11030449

Figure Lengend Snippet: Immunohistochemical analysis of the expression of the PI3K-AKT-mTOR pathway-associated proteins during the first regeneration. The expression of PI3K (p110α), phospho-AKT (Thr308 and Ser473), AKT (pan), phospho-mTOR (Ser2448), and mTOR were analyzed to assess the activation of the PI3K-AKT-mTOR pathway in the thymus during the first regeneration phase. At least three immunostained images were acquired at the same time point, and representative images are shown. ( a ) Irradiated infants; ( b ) irradiated adults. All sections were counterstained with hematoxylin. Scale bar in all panels represents 50 µm.

Article Snippet: To assess activation of the PI3K-AKT-mTOR signaling pathway, FITC-conjugated anti-phospho-AKT (Ser473) (#4071, Cell Signaling Technology, Danvers, MA, USA) was used at 1:7 dilution after incubation for 10–15 min in 100% iced methanol or saponin for permeabilization.

Techniques: Immunohistochemical staining, Expressing, Activation Assay, Irradiation

Journal: iScience

Article Title: STK39 enhances the progression of Cholangiocarcinoma via PI3K/AKT pathway

doi: 10.1016/j.isci.2021.103223

Figure Lengend Snippet: STK39 mediates oncogenic effects on CCA cells through activating the PI3K/AKT signaling pathway (A) Pathway enrichment analysis of differentially expressed genes in RNA-sequence data. (B) Levels of PI3K, AKT, P-PI3K (p85), and P-AKT (Ser473) were examined by western blotting in STK39 stable knockdown or overexpression cells. (C) Stable knockdown of STK39 in QBC-939 cells by shRNA was treated with or without indicated concentrations of IGF-1. The levels of PI3K, AKT, P-PI3K (p85), and P-AKT (Ser473) were determined by western blotting.

Article Snippet: The following antibodies were used for western blotting: anti-SPAK antibody (Abcam, ab128894), anti-PI3K antibody (Abcam, ab189403), anti-P-PI3K antibody (Antibodies-Online, ABIN461415), anti-AKT antibody (Cell Signaling Technology, 4071), anti-P-AKT antibody (Antibodies-Online, ABIN461276), anti-β-actin antibody (Abcam, ab7090), and secondary antibody (Abcam, ab6526)

Techniques: Sequencing, Western Blot, Over Expression, shRNA

Journal: iScience

Article Title: STK39 enhances the progression of Cholangiocarcinoma via PI3K/AKT pathway

doi: 10.1016/j.isci.2021.103223

Figure Lengend Snippet:

Article Snippet: The following antibodies were used for western blotting: anti-SPAK antibody (Abcam, ab128894), anti-PI3K antibody (Abcam, ab189403), anti-P-PI3K antibody (Antibodies-Online, ABIN461415), anti-AKT antibody (Cell Signaling Technology, 4071), anti-P-AKT antibody (Antibodies-Online, ABIN461276), anti-β-actin antibody (Abcam, ab7090), and secondary antibody (Abcam, ab6526)

Techniques: Recombinant, CCK-8 Assay, Purification, In Vitro, Cell Cycle Assay, Sequencing, shRNA, Software, Flow Cytometry

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Rabbit anti-pAKT-S473-AF488 (Clone D9E) , Cell Signaling , Cat#4071S; RRID: AB_1031106.

Techniques: Purification, Recombinant, Software