sars cov 2 rbd protein  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike RBD rFc Recombinant Protein COVID 19 Spike RBD Research
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with the Fc region of rabbit IgG1 at the C terminus
    Catalog Number:
    40592-V31H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological sars cov 2 rbd protein
    A] Cell viability after incubation of Vero cells with imatinib for either 1 or 8 hours. and B] <t>SARS-CoV-2</t> neutralization profile post 1- and 8-hours exposure to imatinib. Inhibition of VSV pseudoparticles for SARS-CoV, SARS-CoV-2, MERS-CoV and VSV(control) after incubation with imatinib in C] Vero cells and D] Vero-TMPRSS2 cells. The red arrow indicates the concentration where no toxicity was observed microscopically anymore (15 nM). E] The association and dissociation curves obtained by BLI reflecting the binding of imatinib (0.78 to 6.25 µM) to immobilized SARS-CoV-2 RBD protein. Data fitted using the 1:1 binding model are shown in black.
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with the Fc region of rabbit IgG1 at the C terminus
    https://www.bioz.com/result/sars cov 2 rbd protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 rbd protein - by Bioz Stars, 2021-04
    94/100 stars

    Images

    1) Product Images from "Bcr-Abl tyrosine kinase inhibitor imatinib as a potential drug for COVID-19"

    Article Title: Bcr-Abl tyrosine kinase inhibitor imatinib as a potential drug for COVID-19

    Journal: bioRxiv

    doi: 10.1101/2020.06.18.158196

    A] Cell viability after incubation of Vero cells with imatinib for either 1 or 8 hours. and B] SARS-CoV-2 neutralization profile post 1- and 8-hours exposure to imatinib. Inhibition of VSV pseudoparticles for SARS-CoV, SARS-CoV-2, MERS-CoV and VSV(control) after incubation with imatinib in C] Vero cells and D] Vero-TMPRSS2 cells. The red arrow indicates the concentration where no toxicity was observed microscopically anymore (15 nM). E] The association and dissociation curves obtained by BLI reflecting the binding of imatinib (0.78 to 6.25 µM) to immobilized SARS-CoV-2 RBD protein. Data fitted using the 1:1 binding model are shown in black.
    Figure Legend Snippet: A] Cell viability after incubation of Vero cells with imatinib for either 1 or 8 hours. and B] SARS-CoV-2 neutralization profile post 1- and 8-hours exposure to imatinib. Inhibition of VSV pseudoparticles for SARS-CoV, SARS-CoV-2, MERS-CoV and VSV(control) after incubation with imatinib in C] Vero cells and D] Vero-TMPRSS2 cells. The red arrow indicates the concentration where no toxicity was observed microscopically anymore (15 nM). E] The association and dissociation curves obtained by BLI reflecting the binding of imatinib (0.78 to 6.25 µM) to immobilized SARS-CoV-2 RBD protein. Data fitted using the 1:1 binding model are shown in black.

    Techniques Used: Incubation, Neutralization, Inhibition, Concentration Assay, Binding Assay

    A] Docked poses of the selected compounds at the receptor-binding domain of SARS-CoV-2 spike protein (inset: conformation of imatinib from molecular dynamics simulations showing important interactions with the receptor at the active site). B] MM-GBSA binding free energies for the selected compounds with negative control DMSO. Error bars indicate standard deviations for sampling from a whole simulation. Tyrosine kinase inhibitors ponatinib and imatinib displayed a high affinity to the RBD of the spike protein.
    Figure Legend Snippet: A] Docked poses of the selected compounds at the receptor-binding domain of SARS-CoV-2 spike protein (inset: conformation of imatinib from molecular dynamics simulations showing important interactions with the receptor at the active site). B] MM-GBSA binding free energies for the selected compounds with negative control DMSO. Error bars indicate standard deviations for sampling from a whole simulation. Tyrosine kinase inhibitors ponatinib and imatinib displayed a high affinity to the RBD of the spike protein.

    Techniques Used: Binding Assay, Negative Control, Sampling

    Related Articles

    Infection:

    Article Title: CoVaccine HT™ adjuvant potentiates robust immune responses to recombinant SARS-CoV-2 Spike S1 immunisation
    Article Snippet: .. SARS-CoV-2 is highly transmissible during both the pre-symptomatic and acute symptomatic phases and the infection fatality rate has been reported as high as 3.4% . .. COVID-19 often develops into severe illness, including pneumonia.

    Article Title: Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
    Article Snippet: .. To evaluate whether these 6 antibodies can recognize SARS-CoV-2 in FFPE specimens, we performed IHC on FFPE pellets of Vero 76 cells infected with SARS-CoV-2. .. We identified 1 rabbit polyclonal antibody against SARS-CoV S (Sino Biological, 40150-T62-COV2) and a mouse monoclonal antibody against SARS-CoV NP (Sino Biological, 40143-MM05) that did not stain uninfected, but stained SARS-CoV-2–infected, FFPE cell pellets ( ).

    other:

    Article Title: Methylene Blue Inhibits In Vitro the SARS-CoV-2 Spike – ACE2 Protein-Protein Interaction – A Mechanism That Can Contribute to Its Antiviral Activity Against COVID-19
    Article Snippet: The plated concentrations of ACE2 receptor were 1.0 μg/mL for SARS-CoV-2 RBD and 2.0 μg/mL for SARS-CoV-2 S1.

    Immunohistochemistry:

    Article Title: Placental SARS-CoV-2 in a patient with mild COVID-19 disease
    Article Snippet: Immunohistochemistry (IHC) staining of SARS-CoV-2 virus in a COVID-19 negative patient, delivery prior to the COVID-19 outbreak (Figure 2a). .. IHC of SARS-CoV-2 from three placental sections (2b: under umbilical cord, 2c: central placental disc, 2d-e: peripheral placental disc at 20x and 40x). .. IHC of cytokeratin-7 (CK-7) marker in control ferret nasal turbinate tissue (Figure 2f).

    Article Title: Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
    Article Snippet: .. To evaluate whether these 6 antibodies can recognize SARS-CoV-2 in FFPE specimens, we performed IHC on FFPE pellets of Vero 76 cells infected with SARS-CoV-2. .. We identified 1 rabbit polyclonal antibody against SARS-CoV S (Sino Biological, 40150-T62-COV2) and a mouse monoclonal antibody against SARS-CoV NP (Sino Biological, 40143-MM05) that did not stain uninfected, but stained SARS-CoV-2–infected, FFPE cell pellets ( ).

    Article Title: Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
    Article Snippet: We also identify 2 commercially available ISH assays that can be used to efficiently detect SARS-CoV-2 RNA in such specimens and develop a dual staining assay using IHC and ISH to detect SARS-CoV-2 S and RNA in the same FFPE section. .. Identification of antibodies suitable for detection of SARS-CoV-2 by IHC and IFA in FFPE specimens.). ..

    Titration:

    Article Title: Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2
    Article Snippet: The dose titer of PsV was determined by infecting ACE-2 and TMPRSS2 expressing 293T cells for 48 h and using Celigo imaging system for imaging and counting virus infected fluorescent cells . .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media. .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media.

    Microneutralization Assay:

    Article Title: Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2
    Article Snippet: The dose titer of PsV was determined by infecting ACE-2 and TMPRSS2 expressing 293T cells for 48 h and using Celigo imaging system for imaging and counting virus infected fluorescent cells . .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media. .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media.

    Expressing:

    Article Title: Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2
    Article Snippet: The dose titer of PsV was determined by infecting ACE-2 and TMPRSS2 expressing 293T cells for 48 h and using Celigo imaging system for imaging and counting virus infected fluorescent cells . .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media. .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media.

    Cell Culture:

    Article Title: Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2
    Article Snippet: The dose titer of PsV was determined by infecting ACE-2 and TMPRSS2 expressing 293T cells for 48 h and using Celigo imaging system for imaging and counting virus infected fluorescent cells . .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media. .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media.

    Concentration Assay:

    Article Title: Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2
    Article Snippet: The dose titer of PsV was determined by infecting ACE-2 and TMPRSS2 expressing 293T cells for 48 h and using Celigo imaging system for imaging and counting virus infected fluorescent cells . .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media. .. Neutralizing Antibody (Nab) Titration Assay for SARS-CoV-2 For microneutralization assay, ACE2-TMPRSS2 expressing 293 T cells were cultured overnight in a half area 96-well plate compatible with Nexcelom Celigo imager at a concentration of 1 ×104 cells per well in 100 µl of complete media.

    Binding Assay:

    Article Title: Bcr-Abl tyrosine kinase inhibitor imatinib as a potential drug for COVID-19
    Article Snippet: The number of infected cells per well were counted using the ImageQuant TL software. .. BLIThe binding kinetics of imatinib on SARS-CoV-2 RBD protein were studied using a BLItz® system (FortéBio). .. Experiments were conducted using the advanced kinetics mode, at room temperature and a buffer system consisting of 1X Kinetics Buffer (FortéBio), 5% anhydrous dimethyl sulfoxide (DMSO; Sigma Aldrich).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
    Article Snippet: .. To evaluate whether these 6 antibodies can recognize SARS-CoV-2 in FFPE specimens, we performed IHC on FFPE pellets of Vero 76 cells infected with SARS-CoV-2. .. We identified 1 rabbit polyclonal antibody against SARS-CoV S (Sino Biological, 40150-T62-COV2) and a mouse monoclonal antibody against SARS-CoV NP (Sino Biological, 40143-MM05) that did not stain uninfected, but stained SARS-CoV-2–infected, FFPE cell pellets ( ).

    Article Title: Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
    Article Snippet: We also identify 2 commercially available ISH assays that can be used to efficiently detect SARS-CoV-2 RNA in such specimens and develop a dual staining assay using IHC and ISH to detect SARS-CoV-2 S and RNA in the same FFPE section. .. Identification of antibodies suitable for detection of SARS-CoV-2 by IHC and IFA in FFPE specimens.). ..

    Immunofluorescence:

    Article Title: Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
    Article Snippet: We also identify 2 commercially available ISH assays that can be used to efficiently detect SARS-CoV-2 RNA in such specimens and develop a dual staining assay using IHC and ISH to detect SARS-CoV-2 S and RNA in the same FFPE section. .. Identification of antibodies suitable for detection of SARS-CoV-2 by IHC and IFA in FFPE specimens.). ..

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  • 94
    Sino Biological sars cov 2 rbd protein
    A] Cell viability after incubation of Vero cells with imatinib for either 1 or 8 hours. and B] <t>SARS-CoV-2</t> neutralization profile post 1- and 8-hours exposure to imatinib. Inhibition of VSV pseudoparticles for SARS-CoV, SARS-CoV-2, MERS-CoV and VSV(control) after incubation with imatinib in C] Vero cells and D] Vero-TMPRSS2 cells. The red arrow indicates the concentration where no toxicity was observed microscopically anymore (15 nM). E] The association and dissociation curves obtained by BLI reflecting the binding of imatinib (0.78 to 6.25 µM) to immobilized SARS-CoV-2 RBD protein. Data fitted using the 1:1 binding model are shown in black.
    Sars Cov 2 Rbd Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 rbd protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 rbd protein - by Bioz Stars, 2021-04
    94/100 stars
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    Image Search Results


    A] Cell viability after incubation of Vero cells with imatinib for either 1 or 8 hours. and B] SARS-CoV-2 neutralization profile post 1- and 8-hours exposure to imatinib. Inhibition of VSV pseudoparticles for SARS-CoV, SARS-CoV-2, MERS-CoV and VSV(control) after incubation with imatinib in C] Vero cells and D] Vero-TMPRSS2 cells. The red arrow indicates the concentration where no toxicity was observed microscopically anymore (15 nM). E] The association and dissociation curves obtained by BLI reflecting the binding of imatinib (0.78 to 6.25 µM) to immobilized SARS-CoV-2 RBD protein. Data fitted using the 1:1 binding model are shown in black.

    Journal: bioRxiv

    Article Title: Bcr-Abl tyrosine kinase inhibitor imatinib as a potential drug for COVID-19

    doi: 10.1101/2020.06.18.158196

    Figure Lengend Snippet: A] Cell viability after incubation of Vero cells with imatinib for either 1 or 8 hours. and B] SARS-CoV-2 neutralization profile post 1- and 8-hours exposure to imatinib. Inhibition of VSV pseudoparticles for SARS-CoV, SARS-CoV-2, MERS-CoV and VSV(control) after incubation with imatinib in C] Vero cells and D] Vero-TMPRSS2 cells. The red arrow indicates the concentration where no toxicity was observed microscopically anymore (15 nM). E] The association and dissociation curves obtained by BLI reflecting the binding of imatinib (0.78 to 6.25 µM) to immobilized SARS-CoV-2 RBD protein. Data fitted using the 1:1 binding model are shown in black.

    Article Snippet: BLIThe binding kinetics of imatinib on SARS-CoV-2 RBD protein were studied using a BLItz® system (FortéBio).

    Techniques: Incubation, Neutralization, Inhibition, Concentration Assay, Binding Assay

    A] Docked poses of the selected compounds at the receptor-binding domain of SARS-CoV-2 spike protein (inset: conformation of imatinib from molecular dynamics simulations showing important interactions with the receptor at the active site). B] MM-GBSA binding free energies for the selected compounds with negative control DMSO. Error bars indicate standard deviations for sampling from a whole simulation. Tyrosine kinase inhibitors ponatinib and imatinib displayed a high affinity to the RBD of the spike protein.

    Journal: bioRxiv

    Article Title: Bcr-Abl tyrosine kinase inhibitor imatinib as a potential drug for COVID-19

    doi: 10.1101/2020.06.18.158196

    Figure Lengend Snippet: A] Docked poses of the selected compounds at the receptor-binding domain of SARS-CoV-2 spike protein (inset: conformation of imatinib from molecular dynamics simulations showing important interactions with the receptor at the active site). B] MM-GBSA binding free energies for the selected compounds with negative control DMSO. Error bars indicate standard deviations for sampling from a whole simulation. Tyrosine kinase inhibitors ponatinib and imatinib displayed a high affinity to the RBD of the spike protein.

    Article Snippet: BLIThe binding kinetics of imatinib on SARS-CoV-2 RBD protein were studied using a BLItz® system (FortéBio).

    Techniques: Binding Assay, Negative Control, Sampling