h10  (Sino Biological)


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    Name:
    Influenza A H10 Hemagglutinin HA Neutralizing Antibody
    Description:
    This antibody was obtained from a mouse immunized with purified recombinant Influenza A H10N8 A Jiangxi Donghu 346 2013 Hemagglutinin HA Catalog 40359 V08B EPI497477 Met1 Asp524 and was produced using recombinant antibody technology
    Catalog Number:
    40359-M001
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Influenza
    Applications:
    Microneutralizaiton(MN),HemagglutininInhibition(HI)
    Immunogen:
    Recombinant Influenza A H10N8 (A/Jiangxi-Donghu/346/2013) Hemagglutinin / HA Protein (Catalog#40359-V08B)
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Sino Biological h10
    Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, <t>H10,</t> H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.
    This antibody was obtained from a mouse immunized with purified recombinant Influenza A H10N8 A Jiangxi Donghu 346 2013 Hemagglutinin HA Catalog 40359 V08B EPI497477 Met1 Asp524 and was produced using recombinant antibody technology
    https://www.bioz.com/result/h10/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h10 - by Bioz Stars, 2021-06
    88/100 stars

    Images

    1) Product Images from "Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses"

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    Journal: Scientific Reports

    doi: 10.1038/srep22045

    Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.
    Figure Legend Snippet: Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Positive Control, Immunofluorescence, Infection, Concentration Assay, HI Assay

    Cross-reactivities between the HA proteins of heterosubtypes of influenza A viruses and antibodies against H7N9. The cross-reactivities were analyzed using ELISA ( A,B ) and Western blot ( C ) with recombinant HA proteins of H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 as antigens. HA proteins were two-fold diluted with a starting dilution of 2,560 ng/ml.
    Figure Legend Snippet: Cross-reactivities between the HA proteins of heterosubtypes of influenza A viruses and antibodies against H7N9. The cross-reactivities were analyzed using ELISA ( A,B ) and Western blot ( C ) with recombinant HA proteins of H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 as antigens. HA proteins were two-fold diluted with a starting dilution of 2,560 ng/ml.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot, Recombinant

    Identification of the cross-reactive regions by Western blot. The lysates of MDCK cells infected with Anhui/1 ( A ), Shanghai/1 ( B ), and Shanghai/2 ( C ) isolates at an MOI of 0.1 were harvested 48 h post infection and probed with antisera to HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16. Antisera against H7N9 HA were used as positive control.
    Figure Legend Snippet: Identification of the cross-reactive regions by Western blot. The lysates of MDCK cells infected with Anhui/1 ( A ), Shanghai/1 ( B ), and Shanghai/2 ( C ) isolates at an MOI of 0.1 were harvested 48 h post infection and probed with antisera to HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16. Antisera against H7N9 HA were used as positive control.

    Techniques Used: Western Blot, Infection, Positive Control

    Related Articles

    Produced:

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses
    Article Snippet: The hemagglutinin activities of the recombinant HA proteins were confirmed using the Octet system (ForteBio, CA) and the hemagglutination assay (data not shown). .. The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological). .. Western blotThree different preparations of MDCK cells were infected with Anhui/1, Shanghai/1, and Shanghai/2 at a multiplicity of infection (MOI) of 0.1 for 48 h. The cells were collected and lysed in RIPA buffer (Roche, Indianapolis, IN) and incubated at 100 °C for 10 min. Western blot assays were performed as described with mouse or rabbit HA antisera as primary antibodies .

    Purification:

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses
    Article Snippet: The hemagglutinin activities of the recombinant HA proteins were confirmed using the Octet system (ForteBio, CA) and the hemagglutination assay (data not shown). .. The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological). .. Western blotThree different preparations of MDCK cells were infected with Anhui/1, Shanghai/1, and Shanghai/2 at a multiplicity of infection (MOI) of 0.1 for 48 h. The cells were collected and lysed in RIPA buffer (Roche, Indianapolis, IN) and incubated at 100 °C for 10 min. Western blot assays were performed as described with mouse or rabbit HA antisera as primary antibodies .

    Recombinant:

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses
    Article Snippet: The hemagglutinin activities of the recombinant HA proteins were confirmed using the Octet system (ForteBio, CA) and the hemagglutination assay (data not shown). .. The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological). .. Western blotThree different preparations of MDCK cells were infected with Anhui/1, Shanghai/1, and Shanghai/2 at a multiplicity of infection (MOI) of 0.1 for 48 h. The cells were collected and lysed in RIPA buffer (Roche, Indianapolis, IN) and incubated at 100 °C for 10 min. Western blot assays were performed as described with mouse or rabbit HA antisera as primary antibodies .

    Affinity Chromatography:

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses
    Article Snippet: The hemagglutinin activities of the recombinant HA proteins were confirmed using the Octet system (ForteBio, CA) and the hemagglutination assay (data not shown). .. The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological). .. Western blotThree different preparations of MDCK cells were infected with Anhui/1, Shanghai/1, and Shanghai/2 at a multiplicity of infection (MOI) of 0.1 for 48 h. The cells were collected and lysed in RIPA buffer (Roche, Indianapolis, IN) and incubated at 100 °C for 10 min. Western blot assays were performed as described with mouse or rabbit HA antisera as primary antibodies .

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    Sino Biological h10
    Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, <t>H10,</t> H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.
    H10, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h10/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h10 - by Bioz Stars, 2021-06
    88/100 stars
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    Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.

    Journal: Scientific Reports

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    doi: 10.1038/srep22045

    Figure Lengend Snippet: Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.

    Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Immunofluorescence, Infection, Concentration Assay, HI Assay

    Cross-reactivities between the HA proteins of heterosubtypes of influenza A viruses and antibodies against H7N9. The cross-reactivities were analyzed using ELISA ( A,B ) and Western blot ( C ) with recombinant HA proteins of H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 as antigens. HA proteins were two-fold diluted with a starting dilution of 2,560 ng/ml.

    Journal: Scientific Reports

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    doi: 10.1038/srep22045

    Figure Lengend Snippet: Cross-reactivities between the HA proteins of heterosubtypes of influenza A viruses and antibodies against H7N9. The cross-reactivities were analyzed using ELISA ( A,B ) and Western blot ( C ) with recombinant HA proteins of H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 as antigens. HA proteins were two-fold diluted with a starting dilution of 2,560 ng/ml.

    Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Recombinant

    Identification of the cross-reactive regions by Western blot. The lysates of MDCK cells infected with Anhui/1 ( A ), Shanghai/1 ( B ), and Shanghai/2 ( C ) isolates at an MOI of 0.1 were harvested 48 h post infection and probed with antisera to HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16. Antisera against H7N9 HA were used as positive control.

    Journal: Scientific Reports

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    doi: 10.1038/srep22045

    Figure Lengend Snippet: Identification of the cross-reactive regions by Western blot. The lysates of MDCK cells infected with Anhui/1 ( A ), Shanghai/1 ( B ), and Shanghai/2 ( C ) isolates at an MOI of 0.1 were harvested 48 h post infection and probed with antisera to HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16. Antisera against H7N9 HA were used as positive control.

    Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).

    Techniques: Western Blot, Infection, Positive Control