40182 atcc 204304  (ATCC)

Journal: Cancer Cell International

Article Title: Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway

doi: 10.1186/s12935-020-01711-z

Figure Lengend Snippet: Effect of emodin on the HA-CD44/RHAMM signaling pathway in lung cancer cell lines. Cells were exposed to 30 µM emodin for 24 h before the western blotting assay. a The suppression rate of emodin on cell viability was plotted vs. suppression rate of emodin on HA secretion. d Representative image of western blotting. c– f The effect of emodin on the protein expression of HAS2, HAS3, CD44, and RHAMM in lung cancer cell lines (Bar chart for western blotting). “*” marks the significant difference (p < 0.05) compare with the control (0 µM or “−”)

Article Snippet: RHAMM siRNA (sc-40181) were obtained from Santa Cruz Biotechnology (Dallas, TX, US).

Techniques: Western Blot, Expressing, Control

Journal: Cancer Cell International

Article Title: Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway

doi: 10.1186/s12935-020-01711-z

Figure Lengend Snippet: The effect of emodin on the HA-CD44/RHAMM signaling pathway knocked down A549 cells. The expression of HAS2 and HAS3 (HAS2/3) or CD44 and RHAMM (CD44/RHAMM) were knocked down in A549 cells. a The effect of emodin on viability. The transfected cells were exposed to 30 µM emodin for 24 h before the MTT assay. b The effect of emodin on HA secretion of transfected A549 cells. c Representative image of western blotting. d – g The effect of emodin on the protein expression of HAS2, HAS3, CD44, and RHAMM in transfected A549 (Bar chart for western blotting). “*” marks the significant difference (p < 0.05) compare with the vehicle control (“−”)

Article Snippet: RHAMM siRNA (sc-40181) were obtained from Santa Cruz Biotechnology (Dallas, TX, US).

Techniques: Expressing, Transfection, MTT Assay, Western Blot, Control

Journal: Cancer Cell International

Article Title: Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway

doi: 10.1186/s12935-020-01711-z

Figure Lengend Snippet: Effect of emodin on the cell cycle of A549. a The cell cycle was analyzed by flow cytometry. “*” indicates a significant difference compared with the vehicle of negative siRNA groups (p < 0.05). b – e Representative data of cell cycle

Article Snippet: RHAMM siRNA (sc-40181) were obtained from Santa Cruz Biotechnology (Dallas, TX, US).

Techniques: Flow Cytometry

Journal: Cancer Cell International

Article Title: Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway

doi: 10.1186/s12935-020-01711-z

Figure Lengend Snippet: Effect of emodin on the expression of cyclin proteins in A549. a Images of western blotting results. b – f The effect of emodin on the protein expression of cyclin A, cyclin B, cyclin C, cyclin D, and cyclin E in A549 (Bar chart for western blotting). “*” marks the significant difference (p < 0.05) compare with the vehicle control. “#” indicates a significant difference (p < 0.05) compare with the vehicle’s negative siRNA control

Article Snippet: RHAMM siRNA (sc-40181) were obtained from Santa Cruz Biotechnology (Dallas, TX, US).

Techniques: Expressing, Western Blot, Control

Journal: The Journal of Biological Chemistry

Article Title: Hyaluronic Acid Receptor CD44 Deficiency Is Associated with Decreased C ryptococcus neoformans Brain Infection *

doi: 10.1074/jbc.M112.353375

Figure Lengend Snippet: Effect of anti-CD44 and anti-RHAMM siRNA treatments on association of C. neoformans cells with mouse BMEC. A, mouse BMEC was treated with siRNA (20 pmol) individually or in combination for 5 h before the in vitro C. neoformans adhesion assay. C. neoformans CPS1 wild-type strain (B-4500FO2) and its isogenic cps1Δ deletion strain C559 were used in parallel. +, anti-CD44 siRNA and/or anti-RHAMM siRNA-treated sample as indicated at the bottom; −, control oligonucleotide (n = 4). In all treatments, the MBMEC without any siRNA treatment was taken as a control for comparison, and the statistical package GraphPad Prism 5 was used to quantify the readings. Significant differences with regard to the controls: *, p < 0.05; **, p < 0.01). B, as a control, a Western blot was used to show the CD44 and RHAMM protein levels after siRNA treatment.

Article Snippet: C , the MetaMorph program associated with the fluorescence microscope was used to scan images with 5–10 random fields from B , and then GraphPad Prism 5 was used to quantify the readings (**, p < 0.01). siRNA Treatment Anti-CD44 siRNA (sc-35534) and anti-RHAMM siRNA (sc-40182) were purchased from Santa Cruz Biotechnology, and a control oligonucleotide (sc-36869) was used in parallel.

Techniques: In Vitro, Cell Adhesion Assay, Control, Comparison, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Hyaluronic Acid Receptor CD44 Deficiency Is Associated with Decreased C ryptococcus neoformans Brain Infection *

doi: 10.1074/jbc.M112.353375

Figure Lengend Snippet: Effect of anti-CD44 and anti-RHAMM siRNA treatments on association of C. neoformans cells with mouse BMEC. A, mouse BMEC was treated with siRNA (20 pmol) individually or in combination for 5 h before the in vitro C. neoformans adhesion assay. C. neoformans CPS1 wild-type strain (B-4500FO2) and its isogenic cps1Δ deletion strain C559 were used in parallel. +, anti-CD44 siRNA and/or anti-RHAMM siRNA-treated sample as indicated at the bottom; −, control oligonucleotide (n = 4). In all treatments, the MBMEC without any siRNA treatment was taken as a control for comparison, and the statistical package GraphPad Prism 5 was used to quantify the readings. Significant differences with regard to the controls: *, p < 0.05; **, p < 0.01). B, as a control, a Western blot was used to show the CD44 and RHAMM protein levels after siRNA treatment.

Article Snippet: Anti-CD44 siRNA (sc-35534) and anti-RHAMM siRNA (sc-40182) were purchased from Santa Cruz Biotechnology, and a control oligonucleotide (sc-36869) was used in parallel.

Techniques: In Vitro, Cell Adhesion Assay, Control, Comparison, Western Blot

Journal: Journal of Biological Chemistry

Article Title: Hyaluronic Acid Receptor CD44 Deficiency Is Associated with Decreased Cryptococcus neoformans Brain Infection

doi: 10.1074/jbc.m112.353375

Figure Lengend Snippet: FIGURE6.Effectofanti-CD44andanti-RHAMMsiRNAtreatmentsonasso- ciation of C. neoformans cells with mouse BMEC. A, mouse BMEC was treated with siRNA (20 pmol) individually or in combination for 5 h before the in vitro C. neoformans adhesion assay. C. neoformans CPS1 wild-type strain (B-4500FO2) and its isogenic cps1 deletion strain C559 were used in parallel. ,anti-CD44siRNAand/oranti-RHAMMsiRNA-treatedsampleasindicatedat the bottom; , control oligonucleotide (n 4). In all treatments, the MBMEC without any siRNA treatment was taken as a control for comparison, and the statistical package GraphPad Prism 5 was used to quantify the readings. Sig- nificant differences with regard to the controls: *, p 0.05; **, p 0.01). B, as a control, a Western blot was used to show the CD44 and RHAMM protein levels after siRNA treatment.

Article Snippet: All samples were examined under a fluorescence microscope at the Congressman Dixon Cellular Imaging Core Facility, Children’s Hospital Los Angeles. siRNA Treatment—Anti-CD44 siRNA (sc-35534) and antiRHAMM siRNA (sc-40182) were purchased from Santa Cruz Biotechnology, and a control oligonucleotide (sc-36869) was used in parallel.

Techniques: In Vitro, Cell Adhesion Assay, Control, Comparison, Western Blot