transfex  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    ATCC transfex
    Transfex, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfex/product/ATCC
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    transfex - by Bioz Stars, 2022-11
    95/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    ATCC transfex transfection kit
    Transfex Transfection Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfex transfection kit/product/ATCC
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    transfex transfection kit - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    94
    ATCC lactobacillus buchneri
    Lactobacillus Buchneri, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactobacillus buchneri/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lactobacillus buchneri - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    94
    ATCC transfex transfection reagent
    A universal strategy for simple and rapid genomic editing of cell populations. ( A ) Drosophila ovarian somatic sheath cells (OSC) represent a unique but delicate model to study Piwi–piRNA mechanism ex vivo. OSC represent somatic follicle cells (FC) of the Drosophila ovary, and express one of the three Drosophila PIWI proteins, Piwi. PiRNAs are generated from long piRNA cluster transcripts by the endonuclease Zucchini (Zuc). Mature Piwi–piRNA silencing complexes transition into the nucleus, recognize nascent transposon transcripts by base-pairing complementarity and induce epigenetic silencing. ( B ) Endogenous tagging of piwi in OSC. An sgRNA was designed to target the endogenous piwi gene in the vicinity of the start codon (ATG). The donor construct for homologous repair contained a FLAG-HA (FH)-tag and a puromycin resistance gene. The FH-tag was fused in frame with piwi's open reading frame (ORF) to generate an endogenously N-terminally tagged protein (eFH-). The puromycin resistance was placed into a synthetic intron and transcribed from the opposite genomic strand. The edited allele is designed to express two independent transcripts: The piwi transcript remains under the control of the endogenous promoter and contains an additional intron and a tag. The mature modified mRNA differs from the wt piwi mRNA only by an additional exon-exon junction and the Flag-HA tag. The second transcript is independently generated from the opposite genomic strand and produces an mRNA encoding a puromycin resistance under the control of an Actin promoter. ( C ) Rapid and simple generation of stably edited OSC:eFH-piwi. Ovarian somatic sheath cells (OSC) were transfected with an expression plasmid for the sgRNA, the Cas9 endonuclease, and the donor plasmid. Cells were treated with SCR7, an inhibitor of non-homologous end joining (NHEJ) to increase the probability for homologous repair. Antibiotic selection with Puromycin (Puro) was started 48 h after <t>transfection.</t> After 2–3 weeks, a puromycin resistant cell population has repopulated the dish
    Transfex Transfection Reagent, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfex transfection reagent/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transfex transfection reagent - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    A universal strategy for simple and rapid genomic editing of cell populations. ( A ) Drosophila ovarian somatic sheath cells (OSC) represent a unique but delicate model to study Piwi–piRNA mechanism ex vivo. OSC represent somatic follicle cells (FC) of the Drosophila ovary, and express one of the three Drosophila PIWI proteins, Piwi. PiRNAs are generated from long piRNA cluster transcripts by the endonuclease Zucchini (Zuc). Mature Piwi–piRNA silencing complexes transition into the nucleus, recognize nascent transposon transcripts by base-pairing complementarity and induce epigenetic silencing. ( B ) Endogenous tagging of piwi in OSC. An sgRNA was designed to target the endogenous piwi gene in the vicinity of the start codon (ATG). The donor construct for homologous repair contained a FLAG-HA (FH)-tag and a puromycin resistance gene. The FH-tag was fused in frame with piwi's open reading frame (ORF) to generate an endogenously N-terminally tagged protein (eFH-). The puromycin resistance was placed into a synthetic intron and transcribed from the opposite genomic strand. The edited allele is designed to express two independent transcripts: The piwi transcript remains under the control of the endogenous promoter and contains an additional intron and a tag. The mature modified mRNA differs from the wt piwi mRNA only by an additional exon-exon junction and the Flag-HA tag. The second transcript is independently generated from the opposite genomic strand and produces an mRNA encoding a puromycin resistance under the control of an Actin promoter. ( C ) Rapid and simple generation of stably edited OSC:eFH-piwi. Ovarian somatic sheath cells (OSC) were transfected with an expression plasmid for the sgRNA, the Cas9 endonuclease, and the donor plasmid. Cells were treated with SCR7, an inhibitor of non-homologous end joining (NHEJ) to increase the probability for homologous repair. Antibiotic selection with Puromycin (Puro) was started 48 h after transfection. After 2–3 weeks, a puromycin resistant cell population has repopulated the dish

    Journal: Nucleic Acids Research

    Article Title: Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells)

    doi: 10.1093/nar/gkac448

    Figure Lengend Snippet: A universal strategy for simple and rapid genomic editing of cell populations. ( A ) Drosophila ovarian somatic sheath cells (OSC) represent a unique but delicate model to study Piwi–piRNA mechanism ex vivo. OSC represent somatic follicle cells (FC) of the Drosophila ovary, and express one of the three Drosophila PIWI proteins, Piwi. PiRNAs are generated from long piRNA cluster transcripts by the endonuclease Zucchini (Zuc). Mature Piwi–piRNA silencing complexes transition into the nucleus, recognize nascent transposon transcripts by base-pairing complementarity and induce epigenetic silencing. ( B ) Endogenous tagging of piwi in OSC. An sgRNA was designed to target the endogenous piwi gene in the vicinity of the start codon (ATG). The donor construct for homologous repair contained a FLAG-HA (FH)-tag and a puromycin resistance gene. The FH-tag was fused in frame with piwi's open reading frame (ORF) to generate an endogenously N-terminally tagged protein (eFH-). The puromycin resistance was placed into a synthetic intron and transcribed from the opposite genomic strand. The edited allele is designed to express two independent transcripts: The piwi transcript remains under the control of the endogenous promoter and contains an additional intron and a tag. The mature modified mRNA differs from the wt piwi mRNA only by an additional exon-exon junction and the Flag-HA tag. The second transcript is independently generated from the opposite genomic strand and produces an mRNA encoding a puromycin resistance under the control of an Actin promoter. ( C ) Rapid and simple generation of stably edited OSC:eFH-piwi. Ovarian somatic sheath cells (OSC) were transfected with an expression plasmid for the sgRNA, the Cas9 endonuclease, and the donor plasmid. Cells were treated with SCR7, an inhibitor of non-homologous end joining (NHEJ) to increase the probability for homologous repair. Antibiotic selection with Puromycin (Puro) was started 48 h after transfection. After 2–3 weeks, a puromycin resistant cell population has repopulated the dish

    Article Snippet: Transfection was done using the TransfeX transfection reagent (ATCC, ACS-4005).

    Techniques: Ex Vivo, Generated, Construct, Modification, Stable Transfection, Transfection, Expressing, Plasmid Preparation, Non-Homologous End Joining, Selection