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steraloids inc 4 cholesten 3 one
4 Cholesten 3 One, supplied by steraloids inc, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 78 stars, based on 1 article reviews
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4 cholesten 3 one - by Bioz Stars, 2020-01
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Article Title: Cholesterol Modulates Cellular TGF-β Responsiveness by Altering TGF-β Binding to TGF-β Receptors
Article Snippet: 25-Hydroxycholesterol, 7-dehydrocholesterol (7-DHC), 7-ketocholestanol, 7-ketocholesterol 5-cholesten-7-one and 4-cholesten-3-one were obtained from Steraloids (Newport, RI). .. Reverse-phase HPLC revealed a mixture of polar products, but no remaining 7-DHC.

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    steraloids inc 4 cholesten 3 one
    <t>7α,12α-Dihydroxy-4-cholesten-3-one</t> and 7α,12α-dihydroxy-5β-cholestan-3-one present in plasma from untreated CTX patient
    4 Cholesten 3 One, supplied by steraloids inc, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 cholesten 3 one/product/steraloids inc
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    4 cholesten 3 one - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    82
    steraloids inc 4 cholesten 3 one cholestenone
    Inhibition of endocytosis by sterol substitution can be reversed by re-addition of cholesterol. (A) Reversibility of PLAP endocytosis. (B) Reversibility of TF endocytosis. Endocytosis experiments were carried out as in substitution studies except that substitution time was shortened to 1 h and followed by a second 1 h substitution incubation with serum-free medium or cholesterol-loaded MβCD in serum-free medium. Con, untreated control cells; Dep, cholesterol-depleted cells; LA, lanosterol substituted in first incubation; AL, allocholesterol substituted in first incubation; EN, <t>4-cholesten-3-one</t> substituted in first incubation; SF, serum-free medium incubated in second incubation; CH, cholesterol-substituted in second incubation. The mean±s.d. from three experiments are shown. * P
    4 Cholesten 3 One Cholestenone, supplied by steraloids inc, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 cholesten 3 one cholestenone/product/steraloids inc
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    4 cholesten 3 one cholestenone - by Bioz Stars, 2020-01
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    steraloids inc 7α hydroxy 4 cholesten 3 one
    <t>7α,12α-Dihydroxy-4-cholesten-3-one</t> and 7α,12α-dihydroxy-5β-cholestan-3-one present in plasma from untreated CTX patient
    7α Hydroxy 4 Cholesten 3 One, supplied by steraloids inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    7α,12α-Dihydroxy-4-cholesten-3-one and 7α,12α-dihydroxy-5β-cholestan-3-one present in plasma from untreated CTX patient

    Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

    Article Title: \u201cProfiling Sterols in Cerebrotendinous Xanthomatosis: Utility of Girard Derivatization and High Resolution Exact Mass LC-ESI-MSn Analysis\u201d

    doi: 10.1016/j.jchromb.2010.11.019

    Figure Lengend Snippet: 7α,12α-Dihydroxy-4-cholesten-3-one and 7α,12α-dihydroxy-5β-cholestan-3-one present in plasma from untreated CTX patient

    Article Snippet: 4,6-Cholestadiene-3-one, 5α-cholestan-3β-ol (5α-cholestanol), 5α-cholestan-3-one, 4-cholesten-3-one (C4-3-one), cholesterol (5-cholesten-3β-ol), 7α-hydroxy-4-cholesten-3-one (C4-7α-ol-3-one) and 3α,7α,12α-trihydroxy-5β-cholestane were from Steraloids (Newport, RI).

    Techniques:

    7α-Hydroxy-4-cholesten-3-one and 4-cholesten-3-one present in plasma from untreated CTX patient and cyp27A1 −/− mice

    Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

    Article Title: \u201cProfiling Sterols in Cerebrotendinous Xanthomatosis: Utility of Girard Derivatization and High Resolution Exact Mass LC-ESI-MSn Analysis\u201d

    doi: 10.1016/j.jchromb.2010.11.019

    Figure Lengend Snippet: 7α-Hydroxy-4-cholesten-3-one and 4-cholesten-3-one present in plasma from untreated CTX patient and cyp27A1 −/− mice

    Article Snippet: 4,6-Cholestadiene-3-one, 5α-cholestan-3β-ol (5α-cholestanol), 5α-cholestan-3-one, 4-cholesten-3-one (C4-3-one), cholesterol (5-cholesten-3β-ol), 7α-hydroxy-4-cholesten-3-one (C4-7α-ol-3-one) and 3α,7α,12α-trihydroxy-5β-cholestane were from Steraloids (Newport, RI).

    Techniques: Mouse Assay

    Fragmentation routes for GP-tagged 3-oxo-4-ene sterols illustrated by 4-cholesten-3-one

    Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

    Article Title: \u201cProfiling Sterols in Cerebrotendinous Xanthomatosis: Utility of Girard Derivatization and High Resolution Exact Mass LC-ESI-MSn Analysis\u201d

    doi: 10.1016/j.jchromb.2010.11.019

    Figure Lengend Snippet: Fragmentation routes for GP-tagged 3-oxo-4-ene sterols illustrated by 4-cholesten-3-one

    Article Snippet: 4,6-Cholestadiene-3-one, 5α-cholestan-3β-ol (5α-cholestanol), 5α-cholestan-3-one, 4-cholesten-3-one (C4-3-one), cholesterol (5-cholesten-3β-ol), 7α-hydroxy-4-cholesten-3-one (C4-7α-ol-3-one) and 3α,7α,12α-trihydroxy-5β-cholestane were from Steraloids (Newport, RI).

    Techniques:

    Inhibition of endocytosis by sterol substitution can be reversed by re-addition of cholesterol. (A) Reversibility of PLAP endocytosis. (B) Reversibility of TF endocytosis. Endocytosis experiments were carried out as in substitution studies except that substitution time was shortened to 1 h and followed by a second 1 h substitution incubation with serum-free medium or cholesterol-loaded MβCD in serum-free medium. Con, untreated control cells; Dep, cholesterol-depleted cells; LA, lanosterol substituted in first incubation; AL, allocholesterol substituted in first incubation; EN, 4-cholesten-3-one substituted in first incubation; SF, serum-free medium incubated in second incubation; CH, cholesterol-substituted in second incubation. The mean±s.d. from three experiments are shown. * P

    Journal: Journal of Cell Science

    Article Title: The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis

    doi: 10.1242/jcs.201731

    Figure Lengend Snippet: Inhibition of endocytosis by sterol substitution can be reversed by re-addition of cholesterol. (A) Reversibility of PLAP endocytosis. (B) Reversibility of TF endocytosis. Endocytosis experiments were carried out as in substitution studies except that substitution time was shortened to 1 h and followed by a second 1 h substitution incubation with serum-free medium or cholesterol-loaded MβCD in serum-free medium. Con, untreated control cells; Dep, cholesterol-depleted cells; LA, lanosterol substituted in first incubation; AL, allocholesterol substituted in first incubation; EN, 4-cholesten-3-one substituted in first incubation; SF, serum-free medium incubated in second incubation; CH, cholesterol-substituted in second incubation. The mean±s.d. from three experiments are shown. * P

    Article Snippet: Epicholesterol (5-cholesten-3α-ol), allocholesterol (4-cholesten-3β-ol), coprostanol, androstenol (5-androsten-3β-ol), 4-cholesten-3-one (cholestenone) and cholesta-4,6-dien-3β-ol were obtained from Steraloids (Newport, RI).

    Techniques: Inhibition, Incubation

    PLAP and TFR protein levels in plasma membrane after cholesterol depletion or its replacement with another sterol. (A) Images of PLAP staining (green) on the cell plasma membrane, counter-stained with CellMask (CM, blue fluorescence). (B) Quantification of PLAP fluorescence intensity. (C) Images of TFR staining (green) on the cell plasma membrane. (D) Quantification of TFR fluorescence intensity. PLAP and TFR on the cell surface were detected by analyzing the immunofluorescence of antibody binding to cells that had been fixed after sterol manipulation. The fluorescence intensity/cell of 50 cells/experiment was then measured. Con, untreated control cells; Dep, cholesterol-depleted cells; AL, allocholesterol; ENON, 4-cholesten-3-one; DES, desmosterol. In B and D, the mean±s.d. are shown from three independent experiments. Scale bars: 50 µm. * P

    Journal: Journal of Cell Science

    Article Title: The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis

    doi: 10.1242/jcs.201731

    Figure Lengend Snippet: PLAP and TFR protein levels in plasma membrane after cholesterol depletion or its replacement with another sterol. (A) Images of PLAP staining (green) on the cell plasma membrane, counter-stained with CellMask (CM, blue fluorescence). (B) Quantification of PLAP fluorescence intensity. (C) Images of TFR staining (green) on the cell plasma membrane. (D) Quantification of TFR fluorescence intensity. PLAP and TFR on the cell surface were detected by analyzing the immunofluorescence of antibody binding to cells that had been fixed after sterol manipulation. The fluorescence intensity/cell of 50 cells/experiment was then measured. Con, untreated control cells; Dep, cholesterol-depleted cells; AL, allocholesterol; ENON, 4-cholesten-3-one; DES, desmosterol. In B and D, the mean±s.d. are shown from three independent experiments. Scale bars: 50 µm. * P

    Article Snippet: Epicholesterol (5-cholesten-3α-ol), allocholesterol (4-cholesten-3β-ol), coprostanol, androstenol (5-androsten-3β-ol), 4-cholesten-3-one (cholestenone) and cholesta-4,6-dien-3β-ol were obtained from Steraloids (Newport, RI).

    Techniques: Staining, Fluorescence, Immunofluorescence, Binding Assay

    Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x DOPC/DPPC/cholesterol/cholestenone with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.

    Journal: Biophysical Journal

    Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles

    doi: 10.1529/biophysj.104.049635

    Figure Lengend Snippet: Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x DOPC/DPPC/cholesterol/cholestenone with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.

    Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI).

    Techniques:

    Fluorescence micrographs of vesicles produced from 1:1:1 DOPC/DPPC/sterol with 0.8 mol % TR-DPPE at 23°C. Vesicles contain ( a ) 25-hydroxycholesterol, ( b ) cholesterol, ( c ) dihydrocholesterol, ( d ) epicholesterol, ( e ) ergosterol, ( f ) no sterol (control), ( g ) androstenolone, ( h ) cholestane, ( i ) cholestenone, and ( j ) coprostanol. As temperature is lowered, phase coexistence is observed between either ( top row ) two liquid phases or ( bottom row ) a solid and liquid phase. With time, liquid phases coalesce to form one bright domain and one dark domain. Scale bars are 10 μ m.

    Journal: Biophysical Journal

    Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles

    doi: 10.1529/biophysj.104.049635

    Figure Lengend Snippet: Fluorescence micrographs of vesicles produced from 1:1:1 DOPC/DPPC/sterol with 0.8 mol % TR-DPPE at 23°C. Vesicles contain ( a ) 25-hydroxycholesterol, ( b ) cholesterol, ( c ) dihydrocholesterol, ( d ) epicholesterol, ( e ) ergosterol, ( f ) no sterol (control), ( g ) androstenolone, ( h ) cholestane, ( i ) cholestenone, and ( j ) coprostanol. As temperature is lowered, phase coexistence is observed between either ( top row ) two liquid phases or ( bottom row ) a solid and liquid phase. With time, liquid phases coalesce to form one bright domain and one dark domain. Scale bars are 10 μ m.

    Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI).

    Techniques: Fluorescence, Produced

    7α,12α-Dihydroxy-4-cholesten-3-one and 7α,12α-dihydroxy-5β-cholestan-3-one present in plasma from untreated CTX patient

    Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

    Article Title: \u201cProfiling Sterols in Cerebrotendinous Xanthomatosis: Utility of Girard Derivatization and High Resolution Exact Mass LC-ESI-MSn Analysis\u201d

    doi: 10.1016/j.jchromb.2010.11.019

    Figure Lengend Snippet: 7α,12α-Dihydroxy-4-cholesten-3-one and 7α,12α-dihydroxy-5β-cholestan-3-one present in plasma from untreated CTX patient

    Article Snippet: 4,6-Cholestadiene-3-one, 5α-cholestan-3β-ol (5α-cholestanol), 5α-cholestan-3-one, 4-cholesten-3-one (C4-3-one), cholesterol (5-cholesten-3β-ol), 7α-hydroxy-4-cholesten-3-one (C4-7α-ol-3-one) and 3α,7α,12α-trihydroxy-5β-cholestane were from Steraloids (Newport, RI).

    Techniques:

    7α-Hydroxy-4-cholesten-3-one and 4-cholesten-3-one present in plasma from untreated CTX patient and cyp27A1 −/− mice

    Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

    Article Title: \u201cProfiling Sterols in Cerebrotendinous Xanthomatosis: Utility of Girard Derivatization and High Resolution Exact Mass LC-ESI-MSn Analysis\u201d

    doi: 10.1016/j.jchromb.2010.11.019

    Figure Lengend Snippet: 7α-Hydroxy-4-cholesten-3-one and 4-cholesten-3-one present in plasma from untreated CTX patient and cyp27A1 −/− mice

    Article Snippet: 4,6-Cholestadiene-3-one, 5α-cholestan-3β-ol (5α-cholestanol), 5α-cholestan-3-one, 4-cholesten-3-one (C4-3-one), cholesterol (5-cholesten-3β-ol), 7α-hydroxy-4-cholesten-3-one (C4-7α-ol-3-one) and 3α,7α,12α-trihydroxy-5β-cholestane were from Steraloids (Newport, RI).

    Techniques: Mouse Assay