lps  (HyTest)


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    Structured Review

    HyTest lps
    Detection of intracellular <t>LPS</t> and cell death in macrophage (6 %) / hepatocyte (94 %) co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). a - b Flow cytometric (FACS) detection of intracellular Francisella <t>tularensis</t> by detection of LPS in a macrophages and b hepatocytes in the mono-cell culture (left side of dashed line) and co-culture (right side of dashed line) (MFI mean fluorescence intensity); c fold change of increase in cell death in macrophages (left side of dashed line) and hepatocytes (right side of dashed line) compared to untreated control in the co-culture; d percentage of detectable macrophages in the co-culture 72 h after infection without (w/o) and with incubation of LVS strain (LVS), F. tularensis holarctica (F. t. hol.) or F. tularensis mediasiatica (F. t. med.). Statistical significance was calculated using student’s t -test ( a - d : * p
    Lps, supplied by HyTest, used in various techniques. Bioz Stars score: 96/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lps/product/HyTest
    Average 96 stars, based on 0 article reviews
    Price from $9.99 to $1999.99
    lps - by Bioz Stars, 2019-09
    96/100 stars

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    1) Product Images from "A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica"

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica

    Journal: BMC Microbiology

    doi: 10.1186/s12866-015-0621-3

    Detection of intracellular LPS and cell death in macrophage (6 %) / hepatocyte (94 %) co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). a - b Flow cytometric (FACS) detection of intracellular Francisella tularensis by detection of LPS in a macrophages and b hepatocytes in the mono-cell culture (left side of dashed line) and co-culture (right side of dashed line) (MFI mean fluorescence intensity); c fold change of increase in cell death in macrophages (left side of dashed line) and hepatocytes (right side of dashed line) compared to untreated control in the co-culture; d percentage of detectable macrophages in the co-culture 72 h after infection without (w/o) and with incubation of LVS strain (LVS), F. tularensis holarctica (F. t. hol.) or F. tularensis mediasiatica (F. t. med.). Statistical significance was calculated using student’s t -test ( a - d : * p
    Figure Legend Snippet: Detection of intracellular LPS and cell death in macrophage (6 %) / hepatocyte (94 %) co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). a - b Flow cytometric (FACS) detection of intracellular Francisella tularensis by detection of LPS in a macrophages and b hepatocytes in the mono-cell culture (left side of dashed line) and co-culture (right side of dashed line) (MFI mean fluorescence intensity); c fold change of increase in cell death in macrophages (left side of dashed line) and hepatocytes (right side of dashed line) compared to untreated control in the co-culture; d percentage of detectable macrophages in the co-culture 72 h after infection without (w/o) and with incubation of LVS strain (LVS), F. tularensis holarctica (F. t. hol.) or F. tularensis mediasiatica (F. t. med.). Statistical significance was calculated using student’s t -test ( a - d : * p

    Techniques Used: Infection, Flow Cytometry, FACS, Cell Culture, Co-Culture Assay, Fluorescence, Incubation

    Related Articles

    Staining:

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
    Article Snippet: Macrophages were stained with antibody against CD45-APC-Cy7 (BD Bioscience, Heidelberg, Germany) and fixed with Inside Stain kit (Miltenyi Biotec, Bergisch Gladbach) according to manufacturer’s recommendations for permeabilization of cells thus allowing detection of F. tularensis LPS. .. Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland). .. Clone FB 11 does not recognize LPS of F. tularensis ssp. mediasiatica.

    Flow Cytometry:

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
    Article Snippet: Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland). .. Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland).

    FACS:

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
    Article Snippet: Paragraph title: FACS analysis ... Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland).

    Cytometry:

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
    Article Snippet: Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland). .. Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland).

    Software:

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
    Article Snippet: Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland). .. Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland).

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  • lps  (HyTest)
    96
    HyTest lps
    Detection of intracellular <t>LPS</t> and cell death in macrophage (6 %) / hepatocyte (94 %) co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). a - b Flow cytometric (FACS) detection of intracellular Francisella <t>tularensis</t> by detection of LPS in a macrophages and b hepatocytes in the mono-cell culture (left side of dashed line) and co-culture (right side of dashed line) (MFI mean fluorescence intensity); c fold change of increase in cell death in macrophages (left side of dashed line) and hepatocytes (right side of dashed line) compared to untreated control in the co-culture; d percentage of detectable macrophages in the co-culture 72 h after infection without (w/o) and with incubation of LVS strain (LVS), F. tularensis holarctica (F. t. hol.) or F. tularensis mediasiatica (F. t. med.). Statistical significance was calculated using student’s t -test ( a - d : * p
    Lps, supplied by HyTest, used in various techniques. Bioz Stars score: 96/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lps/product/HyTest
    Average 96 stars, based on 0 article reviews
    Price from $9.99 to $1999.99
    lps - by Bioz Stars, 2019-09
    96/100 stars
      Buy from Supplier

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    Detection of intracellular LPS and cell death in macrophage (6 %) / hepatocyte (94 %) co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). a - b Flow cytometric (FACS) detection of intracellular Francisella tularensis by detection of LPS in a macrophages and b hepatocytes in the mono-cell culture (left side of dashed line) and co-culture (right side of dashed line) (MFI mean fluorescence intensity); c fold change of increase in cell death in macrophages (left side of dashed line) and hepatocytes (right side of dashed line) compared to untreated control in the co-culture; d percentage of detectable macrophages in the co-culture 72 h after infection without (w/o) and with incubation of LVS strain (LVS), F. tularensis holarctica (F. t. hol.) or F. tularensis mediasiatica (F. t. med.). Statistical significance was calculated using student’s t -test ( a - d : * p

    Journal: BMC Microbiology

    Article Title: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica

    doi: 10.1186/s12866-015-0621-3

    Figure Lengend Snippet: Detection of intracellular LPS and cell death in macrophage (6 %) / hepatocyte (94 %) co-cultures infected with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) and untreated control (hatched bars). a - b Flow cytometric (FACS) detection of intracellular Francisella tularensis by detection of LPS in a macrophages and b hepatocytes in the mono-cell culture (left side of dashed line) and co-culture (right side of dashed line) (MFI mean fluorescence intensity); c fold change of increase in cell death in macrophages (left side of dashed line) and hepatocytes (right side of dashed line) compared to untreated control in the co-culture; d percentage of detectable macrophages in the co-culture 72 h after infection without (w/o) and with incubation of LVS strain (LVS), F. tularensis holarctica (F. t. hol.) or F. tularensis mediasiatica (F. t. med.). Statistical significance was calculated using student’s t -test ( a - d : * p

    Article Snippet: Intracellular Francisellae were stained with 2,5 μg/ml FITC-conjugated antibody versus F. tularensis LPS (clone FB11 - HyTEST Ltd., Turku, Finland).

    Techniques: Infection, Flow Cytometry, FACS, Cell Culture, Co-Culture Assay, Fluorescence, Incubation