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Assessing the effect of D. pannonicus on cell viability and proliferation. ( A ) B16F1 melanoma cells, ( B ) HaCaT cells, and ( C ) human follicle dermal papilla cells (HFDPCs) were treated with D. pannonicus extract for 24 h. After treatment, cell viability was assessed using the MTT assay. ( D ) B16F1 melanoma cells were treated with D. pannonicus extract in the presence of α-MSH for 3 days. After treatment, the trypan blue exclusion test was performed, and the cell proliferation rate was assessed. The data are shown as mean ± SEM of at least three independent experiments. Statistical significance is indicated as ** p < 0.01.

Journal: Life

Article Title: Desmodesmus pannonicus Water Extract Inhibits Melanin Synthesis and Promotes Wound Healing

doi: 10.3390/life14121542

Figure Lengend Snippet: Assessing the effect of D. pannonicus on cell viability and proliferation. ( A ) B16F1 melanoma cells, ( B ) HaCaT cells, and ( C ) human follicle dermal papilla cells (HFDPCs) were treated with D. pannonicus extract for 24 h. After treatment, cell viability was assessed using the MTT assay. ( D ) B16F1 melanoma cells were treated with D. pannonicus extract in the presence of α-MSH for 3 days. After treatment, the trypan blue exclusion test was performed, and the cell proliferation rate was assessed. The data are shown as mean ± SEM of at least three independent experiments. Statistical significance is indicated as ** p < 0.01.

Article Snippet: HFDPCs (PromoCell, Heidelberg, Germany) were cultured in Follicle Dermal Papilla Cell Growth Medium (PromoCell).

Techniques: MTT Assay