Journal: Computational and Structural Biotechnology Journal

Article Title: Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

doi: 10.1016/j.csbj.2022.11.054

Figure Lengend Snippet: The levels of immune response related proteins were elevated in the SGs of DIO mice. (A-C) Immunohistochemical analysis of CD4 (A), CD8 (B) and CD79A (C) in the SMG, SLG and PG from the NC and DIO mice (week-35), respectively. Arrow points to the representative region with elevated protein positive signals in the DIO mice. (D) Western blot analysis of CD4, CD79A, CD8A, MUC20, MUC13 and α-Amylase in the SGs of three NC mice and three DIO mice (week-35), respectively. β-Actin: loading control.

Article Snippet: Antibodies against CD4 (ER1706-80, HuaBio, Hangzhou), CD8A (0108–7, HuaBio, Hangzhou), CD79A (EM1902-29, HuaBio, Hangzhou), MUC13 (ER1913-37, HuaBio, Hangzhou), MUC20 (A15968, ABclonal,Wuhan), α-Amylase (3796,Cell Signaling Technology, America) andβ-Actin (AC026, ABclonal, Wuhan) were purchased.

Techniques: Immunohistochemical staining, Western Blot

Journal: Computational and Structural Biotechnology Journal

Article Title: Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

doi: 10.1016/j.csbj.2022.11.054

Figure Lengend Snippet: SGs of the DIO mice produced less mucin. (A-C) Images showing the alcian blue staining of sections of the SMG (A), SLG (B) and PG (C) dissected from NC-2, NC-3, NC-7, NC-8, DIO-2 and DIO-3, DIO-7 or DIO-8 as indicated. Statistic comparison of the relative staining intensity (ratio of the blue color pixels against total color pixels) between NC and DIO mice is shown on the right. Blue staining signals of 2 sections from each NC (NC-2, NC-3, NC-7, and NC-8) and DIO (DIO-2, DIO-3, DIO-7 and DIO-8) mice were acquired for statistic analysis. (D-F) Comparing the pilocarpine hydrochloride stimulated saliva flow rate (n = 3) (D), peroxidase (POD) activity (n = 3) (E) and proteins levels of MUC13 and α-Amylase in the stimulated saliva between three NC and three DIO mice (week-35). For (F), equal amount of the loaded protein samples was determined based on the protein concentration measured using the Bradford method. ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Antibodies against CD4 (ER1706-80, HuaBio, Hangzhou), CD8A (0108–7, HuaBio, Hangzhou), CD79A (EM1902-29, HuaBio, Hangzhou), MUC13 (ER1913-37, HuaBio, Hangzhou), MUC20 (A15968, ABclonal,Wuhan), α-Amylase (3796,Cell Signaling Technology, America) andβ-Actin (AC026, ABclonal, Wuhan) were purchased.

Techniques: Produced, Staining, Activity Assay, Protein Concentration

Journal: Nature Communications

Article Title: κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis

doi: 10.1038/s41467-020-17226-0

Figure Lengend Snippet: a Resected tumours of 10-week-old 1SKO/R and pDKO/R mice (left) and exemplary haematoxylin/eosin staining of a section of 10-week-old pDKO/R mice (right). Scale bar: 500 µm. b Fluorescent IHC staining of CK19 (magenta) and amylase1 (green) in pancreatic sections of 1SKO/R and pDKO/R littermate mice (4 weeks old). Scale bar: 200 µm. c FACS analysis of pancreatic cell populations. Acinar cells were excluded from analysis based on FSC/SSC and residual cells analysed for ductal (MIC-1C3 + ) and acinar cell markers (MPx1 + ) by surface staining. d Fluorescent IHC staining of Cpa1 (magenta, acinar cell marker), Sox9 (green) and nuclei (DAPI, blue) in paraffin sections of 1SKO/R and pDKO/R littermate mice (4 weeks old). Scale bar: 200 µm.

Article Snippet: Primary antibodies and dilutions used: anti-CK19 (1:100, Santa Cruz, sc-33111), anti-Amylase1 (1:100, Cell Signalling, D55H10), anti-Sox9 (1:100, Cell Signalling, D8G8H), anti-Cpa1 (1:100, Novus, AF2765), anti-phospho-p44/42 (1:200, Cell Signalling, Thr202/Tyr204), anti-Ki67 (1:100, Cell Signalling, 12202, clone D3B5) and anti-phospho-Akt substrate (1:100, Cell Signalling 9611).

Techniques: Staining, Immunohistochemistry, Marker

Journal: Nature Communications

Article Title: κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis

doi: 10.1038/s41467-020-17226-0

Figure Lengend Snippet: a Experimental set-up of acute pancreatitis model. b The percentage of pancreatic tissue affected by ADM after cerulein injections was scored in haematoxylin/eosin-stained sections (score 0: 0–2%; score 1: 2–50%; score 2: >50%). Data are presented as mean and single values. Data represent the collected results from two (9- and 24-h time points) or three (48-h and 21-d time points) independent experiments. c , d Sections of pancreata from mice with the indicated genotypes 48 h ( c ) and 21 d ( d ) after cerulein or saline injections were stained with haematoxylin/eosin or antibodies against amylase1 or Sox9. White arrows indicate Sox9 expression in ductal cells. Black arrows indicate Sox9 expression in acinar cells. Scale bar haematoxylin/eosin and amylase: 50 µm. Scale bar: Sox9: 100 µm.

Article Snippet: Primary antibodies and dilutions used: anti-CK19 (1:100, Santa Cruz, sc-33111), anti-Amylase1 (1:100, Cell Signalling, D55H10), anti-Sox9 (1:100, Cell Signalling, D8G8H), anti-Cpa1 (1:100, Novus, AF2765), anti-phospho-p44/42 (1:200, Cell Signalling, Thr202/Tyr204), anti-Ki67 (1:100, Cell Signalling, 12202, clone D3B5) and anti-phospho-Akt substrate (1:100, Cell Signalling 9611).

Techniques: Staining, Expressing