3730xl dna analyzer  (Thermo Fisher)


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    3730xl DNA Analyzer factory refurbished
    Description:
    The 96 capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis Use this for DNA fragment analysis applications such as microsatellites AFLP SNP analysis mutation detection and traditional DNA sequencing Get the highest quality data at a low cost per sample This instrument is refurbished by the original manufacturer to meet both manufacturing and product specifications This system includes the refurbished instrument new Windows computer Data Collection software choice of one analysis software package installation by an Applied Biosystems “5 Step Certified Field Service Engineer chemistry kit training new parts and a one year warranty Availability may be limited • Higher optical sensitivity and advanced polymers enable you to obtain higher quality sequencing data for less • Multiple automation features decrease costly human errors • Optimized polymers increase your productivity without compromising your results • Perform a wide variety of sequencing and fragment analysis applications including resequencing microsatellite analysis AFLP LOH SSCP SNP screening and SNP validation Large Scale DNA Analysis on a Small Scale BudgetBy combining advances in automation with innovative optics and proprietary reagents to increase throughput yield high quality data and minimize reagent consumption the 3730xl Analyzer provides your laboratory and production facility with faster better cheaper analysis Streamline Your Workflow The 3730xl DNA Analyzer system is engineered for highly reliable unattended operation of up to 48 hours applies to modules with run times longer than 30 minutes To minimize the need for operator intervention and decrease the risk of human error automation features include an integrated plate stacker internal bar code reader and onboard polymer delivery system Increase Productivity The high signal to noise ratio ensures you get high quality data even when you use low concentration samples and reagents Labor saving automation features minimize hands on time and enable you to analyze more data more efficiently Get the Highest Quality Sequencing and Genotyping DataThe enhanced optical design provides a higher signal to noise ratio and a more uniform signal profile across the array This design combined with our new advanced polymer enables the longest read lengths of any available system and provides enhanced color balance for streamlined genotyping sample handling In addition exceptional sensitivity enables higher success rates across a wide range of sample template types and concentrations One Instrument Multiple Applications With the 3730xl DNA Analyzer you get the highest quality data from a wide array of applications In addition to being ideal for high throughput sequencing the analyzer s optimized application assays instrument and analysis software also provide a complete solution for genotyping and resequencing Integrated Data Analysis Tools Reduce Time to ResultsThe 3730xl DNA Analyzer software suite allows you to generate more meaningful data with less work This system s labor saving software suite includes • Data Collection supplied with the instrument Manages your instrument setup controls instrument operations allows real time data visualization and performs diagnostics • Sequencing Analysis Software Designed to base call assign quality values trim display edit and print DNA sequencing data using the KB basecaller • Seqscape Provides everything you need to perform resequencing applications such as VariantSEQr Resequencing System • GeneMapper Enables configurable automated allele calling a plus for high throughput genotyping and includes tools for SNPlex data analysis New features include • Auto analysis with GeneMapper and SeqScape • Flexibility to use any choice in dye set option • Tools to assist with regulatory and compliance requirements In the United States this assists with FDA 21CFR part 11 • Additional optimized run modules covering more applications • Support for fragment analysis applications on the 96 capillary arrayThe instrument comes with a one year limited warranty on parts and labor For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    3730xlr
    Price:
    None
    Applications:
    Amplified Fragment Length Polymorphism-AFLP®|DNA Sequencing|Fragment Analysis|Genotyping & Genomic Profiling|Microsatellite Marker Analysis|Resequencing|SNP Genotyping by Fragment-Analysis|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Microsatellite Analysis|Microsatellite STR Analysis|Gene Expression Analysis & Genotyping|SNP Genotyping|Sequencing|Genotyping Instruments, Software & Calibration
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher 3730xl dna analyzer
    Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI <t>3730XL</t> for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).
    The 96 capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis Use this for DNA fragment analysis applications such as microsatellites AFLP SNP analysis mutation detection and traditional DNA sequencing Get the highest quality data at a low cost per sample This instrument is refurbished by the original manufacturer to meet both manufacturing and product specifications This system includes the refurbished instrument new Windows computer Data Collection software choice of one analysis software package installation by an Applied Biosystems “5 Step Certified Field Service Engineer chemistry kit training new parts and a one year warranty Availability may be limited • Higher optical sensitivity and advanced polymers enable you to obtain higher quality sequencing data for less • Multiple automation features decrease costly human errors • Optimized polymers increase your productivity without compromising your results • Perform a wide variety of sequencing and fragment analysis applications including resequencing microsatellite analysis AFLP LOH SSCP SNP screening and SNP validation Large Scale DNA Analysis on a Small Scale BudgetBy combining advances in automation with innovative optics and proprietary reagents to increase throughput yield high quality data and minimize reagent consumption the 3730xl Analyzer provides your laboratory and production facility with faster better cheaper analysis Streamline Your Workflow The 3730xl DNA Analyzer system is engineered for highly reliable unattended operation of up to 48 hours applies to modules with run times longer than 30 minutes To minimize the need for operator intervention and decrease the risk of human error automation features include an integrated plate stacker internal bar code reader and onboard polymer delivery system Increase Productivity The high signal to noise ratio ensures you get high quality data even when you use low concentration samples and reagents Labor saving automation features minimize hands on time and enable you to analyze more data more efficiently Get the Highest Quality Sequencing and Genotyping DataThe enhanced optical design provides a higher signal to noise ratio and a more uniform signal profile across the array This design combined with our new advanced polymer enables the longest read lengths of any available system and provides enhanced color balance for streamlined genotyping sample handling In addition exceptional sensitivity enables higher success rates across a wide range of sample template types and concentrations One Instrument Multiple Applications With the 3730xl DNA Analyzer you get the highest quality data from a wide array of applications In addition to being ideal for high throughput sequencing the analyzer s optimized application assays instrument and analysis software also provide a complete solution for genotyping and resequencing Integrated Data Analysis Tools Reduce Time to ResultsThe 3730xl DNA Analyzer software suite allows you to generate more meaningful data with less work This system s labor saving software suite includes • Data Collection supplied with the instrument Manages your instrument setup controls instrument operations allows real time data visualization and performs diagnostics • Sequencing Analysis Software Designed to base call assign quality values trim display edit and print DNA sequencing data using the KB basecaller • Seqscape Provides everything you need to perform resequencing applications such as VariantSEQr Resequencing System • GeneMapper Enables configurable automated allele calling a plus for high throughput genotyping and includes tools for SNPlex data analysis New features include • Auto analysis with GeneMapper and SeqScape • Flexibility to use any choice in dye set option • Tools to assist with regulatory and compliance requirements In the United States this assists with FDA 21CFR part 11 • Additional optimized run modules covering more applications • Support for fragment analysis applications on the 96 capillary arrayThe instrument comes with a one year limited warranty on parts and labor For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/3730xl dna analyzer/product/Thermo Fisher
    Average 99 stars, based on 6868 article reviews
    Price from $9.99 to $1999.99
    3730xl dna analyzer - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "A deletion in GDF7 is associated with a heritable forebrain commissural malformation concurrent with ventriculomegaly and interhemispheric cysts in cats"

    Article Title: A deletion in GDF7 is associated with a heritable forebrain commissural malformation concurrent with ventriculomegaly and interhemispheric cysts in cats

    Journal: bioRxiv

    doi: 10.1101/2020.05.12.091686

    Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI 3730XL for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).
    Figure Legend Snippet: Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI 3730XL for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).

    Techniques Used: Variant Assay, Sequencing, Fluorescence

    2) Product Images from "A deletion in GDF7 is associated with a heritable forebrain commissural malformation concurrent with ventriculomegaly and interhemispheric cysts in cats"

    Article Title: A deletion in GDF7 is associated with a heritable forebrain commissural malformation concurrent with ventriculomegaly and interhemispheric cysts in cats

    Journal: bioRxiv

    doi: 10.1101/2020.05.12.091686

    Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI 3730XL for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).
    Figure Legend Snippet: Variant validation by Sanger sequencing and fragment analysis. Sanger sequence of a wildtype and homozygous affected cat for the 7 bp GDF7 variant (boxed region). (B) Fluorescence-based fragment analysis using an ABI 3730XL for the GDF7 variant. Left – homozygous wildtype with 294 bp fragment, middle – heterozygous with 287 and 294 bp fragments, and right – affected with 287 bp fragment. LIZ standard (Applied Biosystems, Foster City, CA, USA).

    Techniques Used: Variant Assay, Sequencing, Fluorescence

    Related Articles

    Clone Assay:

    Article Title: Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity
    Article Snippet: .. Clones with a correct product size of 1 Kb were verified by sequence analysis to confirm each point mutation (University of Michigan Sequence Core, Applied Biosystems 3730xl DNA Analyzer). .. Correct site-directed PefE plasmids were transformed into P . mirabilis HI4320 immunity mutant 9C1 and P . mirabilis BB2000 for immunity complementation experiments.

    Article Title: Urinary Tract Effects of HPSE2 Mutations
    Article Snippet: .. Amplicons derived from patient and control DNA were cloned into TOPO pCR2.1 vector and, following verification of correct sequence (BigDye Terminator v3.1Cycle Sequencing Kit, analyzed on the Applied Biosystems 3730xl DNA analyzer), were subsequently subcloned as NdeI digested fragments into the pTB vector using standard procedures. .. DNA of the mutant and wild-type expression constructs was prepared from bacterial cultures using the Qiagen mini-prep kit.

    Mutagenesis:

    Article Title: Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity
    Article Snippet: .. Clones with a correct product size of 1 Kb were verified by sequence analysis to confirm each point mutation (University of Michigan Sequence Core, Applied Biosystems 3730xl DNA Analyzer). .. Correct site-directed PefE plasmids were transformed into P . mirabilis HI4320 immunity mutant 9C1 and P . mirabilis BB2000 for immunity complementation experiments.

    Purification:

    Article Title: A C17T polymorphism in the mu opiate receptor is associated with quantitative measures of drug use in African-American women
    Article Snippet: .. Prior to electrophoresis on an ABI 3730xl DNA Analyzer, the sequencing reactions were purified using BigDye® XTerminator™ purification kit (the sequencing and purification kits, as well as the 3730xl instrument are from Applied Biosystems). ..

    Electrophoresis:

    Article Title: A C17T polymorphism in the mu opiate receptor is associated with quantitative measures of drug use in African-American women
    Article Snippet: .. Prior to electrophoresis on an ABI 3730xl DNA Analyzer, the sequencing reactions were purified using BigDye® XTerminator™ purification kit (the sequencing and purification kits, as well as the 3730xl instrument are from Applied Biosystems). ..

    Sequencing:

    Article Title: Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond
    Article Snippet: .. Sanger sequencing was performed at the University of Calgary Core DNA Services using Applied Biosystems 3730xl (96 capillary) genetic analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity
    Article Snippet: .. Clones with a correct product size of 1 Kb were verified by sequence analysis to confirm each point mutation (University of Michigan Sequence Core, Applied Biosystems 3730xl DNA Analyzer). .. Correct site-directed PefE plasmids were transformed into P . mirabilis HI4320 immunity mutant 9C1 and P . mirabilis BB2000 for immunity complementation experiments.

    Article Title: Urinary Tract Effects of HPSE2 Mutations
    Article Snippet: .. Amplicons derived from patient and control DNA were cloned into TOPO pCR2.1 vector and, following verification of correct sequence (BigDye Terminator v3.1Cycle Sequencing Kit, analyzed on the Applied Biosystems 3730xl DNA analyzer), were subsequently subcloned as NdeI digested fragments into the pTB vector using standard procedures. .. DNA of the mutant and wild-type expression constructs was prepared from bacterial cultures using the Qiagen mini-prep kit.

    Article Title: Variants associated with Gaucher disease in multiple system atrophy
    Article Snippet: .. Nucleotide sequence analysis of GBA Polymerase chain reaction products were subjected to direct nucleotide sequence analysis of coding sequences and flanking splice sites of GBA with a DNA analyzer, 3730xl (Life Technologies, Carlsbad, CA). .. Three primer pairs were designed selectively to amplify GBA but not its pseudogene, as previously described., Analysis of sequence traces was achieved using Variant Reporter v1.1 (Life Technologies) and by manual inspection of electropherograms.

    Article Title: A C17T polymorphism in the mu opiate receptor is associated with quantitative measures of drug use in African-American women
    Article Snippet: .. Prior to electrophoresis on an ABI 3730xl DNA Analyzer, the sequencing reactions were purified using BigDye® XTerminator™ purification kit (the sequencing and purification kits, as well as the 3730xl instrument are from Applied Biosystems). ..

    Article Title: Identification of a novel CRYAB mutation associated with autosomal recessive juvenile cataract in a Saudi family
    Article Snippet: .. DNA sequence analysis was performed by dye termination sequencing (BigDye Terminator Cycle Sequencing v3.1 kit and the Prism 3730XL Genetic Analyzer; Applied Biosystems, Foster City, CA). .. DNA sequences were analyzed using the Seqman program of the DNASTAR analysis package (Lasergene, Madison, WI).

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: .. Maxiprep of the DNA plasmids was performed (Qiagen, Valencia, CA), and the DNA sequence of each plasmid was verified via DNA sequencing (with the BigDye terminator methodology and ABI 3730xl genetic analyzers; both from Applied Biosystems, Foster City, CA) and alignment (Sequencher program 5.0; Gene Codes, Ann Arbor, MI). .. Plasmids were linearized via restriction enzyme digest (New England Biolabs, Ipswich, MA) and in vitro transcribed using the T7-Scribe Standard RNA IVT kit (Cell Script, Madison, WI).

    Plasmid Preparation:

    Article Title: Urinary Tract Effects of HPSE2 Mutations
    Article Snippet: .. Amplicons derived from patient and control DNA were cloned into TOPO pCR2.1 vector and, following verification of correct sequence (BigDye Terminator v3.1Cycle Sequencing Kit, analyzed on the Applied Biosystems 3730xl DNA analyzer), were subsequently subcloned as NdeI digested fragments into the pTB vector using standard procedures. .. DNA of the mutant and wild-type expression constructs was prepared from bacterial cultures using the Qiagen mini-prep kit.

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: .. Maxiprep of the DNA plasmids was performed (Qiagen, Valencia, CA), and the DNA sequence of each plasmid was verified via DNA sequencing (with the BigDye terminator methodology and ABI 3730xl genetic analyzers; both from Applied Biosystems, Foster City, CA) and alignment (Sequencher program 5.0; Gene Codes, Ann Arbor, MI). .. Plasmids were linearized via restriction enzyme digest (New England Biolabs, Ipswich, MA) and in vitro transcribed using the T7-Scribe Standard RNA IVT kit (Cell Script, Madison, WI).

    Polymerase Chain Reaction:

    Article Title: Variants associated with Gaucher disease in multiple system atrophy
    Article Snippet: .. Nucleotide sequence analysis of GBA Polymerase chain reaction products were subjected to direct nucleotide sequence analysis of coding sequences and flanking splice sites of GBA with a DNA analyzer, 3730xl (Life Technologies, Carlsbad, CA). .. Three primer pairs were designed selectively to amplify GBA but not its pseudogene, as previously described., Analysis of sequence traces was achieved using Variant Reporter v1.1 (Life Technologies) and by manual inspection of electropherograms.

    Article Title: Evaluating Imaging Biomarkers of Acquired Resistance to Targeted EGFR Therapy in Xenograft Models of Human Head and Neck Squamous Cell Carcinoma
    Article Snippet: .. Cells were routinely screened for mycoplasma using PCR and had STR profiles (obtained using a GenePrint® 10 kit, Promega, Southampton, UK and a 3730xl DNA analyser, Applied Biosystems, Warrington, UK) identical to parental CAL 27 cells. ..

    Derivative Assay:

    Article Title: Urinary Tract Effects of HPSE2 Mutations
    Article Snippet: .. Amplicons derived from patient and control DNA were cloned into TOPO pCR2.1 vector and, following verification of correct sequence (BigDye Terminator v3.1Cycle Sequencing Kit, analyzed on the Applied Biosystems 3730xl DNA analyzer), were subsequently subcloned as NdeI digested fragments into the pTB vector using standard procedures. .. DNA of the mutant and wild-type expression constructs was prepared from bacterial cultures using the Qiagen mini-prep kit.

    DNA Sequencing:

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: .. Maxiprep of the DNA plasmids was performed (Qiagen, Valencia, CA), and the DNA sequence of each plasmid was verified via DNA sequencing (with the BigDye terminator methodology and ABI 3730xl genetic analyzers; both from Applied Biosystems, Foster City, CA) and alignment (Sequencher program 5.0; Gene Codes, Ann Arbor, MI). .. Plasmids were linearized via restriction enzyme digest (New England Biolabs, Ipswich, MA) and in vitro transcribed using the T7-Scribe Standard RNA IVT kit (Cell Script, Madison, WI).

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  • 99
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    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6868 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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