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(A) Representative SEM images of fixed human primary <t>macrophages</t> seeded on top of different porous transwell inserts (0.4, 1, 3 and 8 µm pores) or produced membranes. Cells that were able to migrate through the pores can be seen in the representative bottom image. Scale bar represents 10 µm in all images (B) Quantification of migrated human primary macrophages on the bottom of 24-well plate. Data presented as mean ± SD, n = 3. (C) Representative light microscopy images of well plate bottoms of 3 and 8 µm transwell after 24 h. Scale bar represents 200 µm.
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(A) Representative SEM images of fixed human primary <t>macrophages</t> seeded on top of different porous transwell inserts (0.4, 1, 3 and 8 µm pores) or produced membranes. Cells that were able to migrate through the pores can be seen in the representative bottom image. Scale bar represents 10 µm in all images (B) Quantification of migrated human primary macrophages on the bottom of 24-well plate. Data presented as mean ± SD, n = 3. (C) Representative light microscopy images of well plate bottoms of 3 and 8 µm transwell after 24 h. Scale bar represents 200 µm.
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(A) Representative SEM images of fixed human primary macrophages seeded on top of different porous transwell inserts (0.4, 1, 3 and 8 µm pores) or produced membranes. Cells that were able to migrate through the pores can be seen in the representative bottom image. Scale bar represents 10 µm in all images (B) Quantification of migrated human primary macrophages on the bottom of 24-well plate. Data presented as mean ± SD, n = 3. (C) Representative light microscopy images of well plate bottoms of 3 and 8 µm transwell after 24 h. Scale bar represents 200 µm.

Journal: PLOS One

Article Title: Supporting islet function in a PVDF membrane based macroencapsulation delivery device by solvent non-solvent casting using PVP

doi: 10.1371/journal.pone.0298114

Figure Lengend Snippet: (A) Representative SEM images of fixed human primary macrophages seeded on top of different porous transwell inserts (0.4, 1, 3 and 8 µm pores) or produced membranes. Cells that were able to migrate through the pores can be seen in the representative bottom image. Scale bar represents 10 µm in all images (B) Quantification of migrated human primary macrophages on the bottom of 24-well plate. Data presented as mean ± SD, n = 3. (C) Representative light microscopy images of well plate bottoms of 3 and 8 µm transwell after 24 h. Scale bar represents 200 µm.

Article Snippet: Human primary macrophages (Celprogen, United States) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Thermo Fisher Scientific, the Netherlands) supplemented with 10% (v/v) FBS (Sigma-Aldrich, the Netherlands).

Techniques: Produced, Light Microscopy