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human primary macrophages  (Celprogen Inc)
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Celprogen Inc human primary macrophages
Fig. 1. LPS increased HBO1 protein levels in THP-1 cells and Human primary <t>macrophages.</t> (A, B) THP-1 cells were treated with LPS in a range of con- centrations as indicated for 6 h. Cell lysates were analyzed using immunoblotting for HBO1 or β-actin. The densitometry results of A were plotted in B. (C, D) THP-1 cells were treated with LPS (125 ng/mL) at varying time points. Cells lysates were analyzed with HBO1 or β-actin immunoblotting. The densitometry results of C were plotted in D. (E, F) Human primary macrophages were treated with LPS in a range of concentrations for 6 h. Cell lysates were analyzed using HBO1 or β-actin immunoblotting. The densitometry results of E were plotted in F. (G, H) Human primary macrophages were treated with LPS (125 ng/mL) for different time points. Cells lysates were analyzed with HBO1 or β-actin immunoblotting. The densitometry results of G were plotted in H. Data re- presents n = 3 separate experiments. Graphs show mean ± SD, “*” denotes p < 0.05 and “**” denotes p < 0.01.
Human Primary Macrophages, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav asyn s87a/s129a (plasmid #36070)  (Addgene inc)
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Fig. 1. LPS increased HBO1 protein levels in THP-1 cells and Human primary macrophages. (A, B) THP-1 cells were treated with LPS in a range of con- centrations as indicated for 6 h. Cell lysates were analyzed using immunoblotting for HBO1 or β-actin. The densitometry results of A were plotted in B. (C, D) THP-1 cells were treated with LPS (125 ng/mL) at varying time points. Cells lysates were analyzed with HBO1 or β-actin immunoblotting. The densitometry results of C were plotted in D. (E, F) Human primary macrophages were treated with LPS in a range of concentrations for 6 h. Cell lysates were analyzed using HBO1 or β-actin immunoblotting. The densitometry results of E were plotted in F. (G, H) Human primary macrophages were treated with LPS (125 ng/mL) for different time points. Cells lysates were analyzed with HBO1 or β-actin immunoblotting. The densitometry results of G were plotted in H. Data re- presents n = 3 separate experiments. Graphs show mean ± SD, “*” denotes p < 0.05 and “**” denotes p < 0.01.

Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

Article Title: LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells.

doi: 10.1016/j.bbagrm.2018.08.001

Figure Lengend Snippet: Fig. 1. LPS increased HBO1 protein levels in THP-1 cells and Human primary macrophages. (A, B) THP-1 cells were treated with LPS in a range of con- centrations as indicated for 6 h. Cell lysates were analyzed using immunoblotting for HBO1 or β-actin. The densitometry results of A were plotted in B. (C, D) THP-1 cells were treated with LPS (125 ng/mL) at varying time points. Cells lysates were analyzed with HBO1 or β-actin immunoblotting. The densitometry results of C were plotted in D. (E, F) Human primary macrophages were treated with LPS in a range of concentrations for 6 h. Cell lysates were analyzed using HBO1 or β-actin immunoblotting. The densitometry results of E were plotted in F. (G, H) Human primary macrophages were treated with LPS (125 ng/mL) for different time points. Cells lysates were analyzed with HBO1 or β-actin immunoblotting. The densitometry results of G were plotted in H. Data re- presents n = 3 separate experiments. Graphs show mean ± SD, “*” denotes p < 0.05 and “**” denotes p < 0.01.

Article Snippet: Human primary macrophages and their culture medium were from Celprogen (San Pedro, CA) and maintained in a 37 °C incubator in the presence of 5% CO2.

Techniques: Western Blot