moellerella wisconsensis atcc 35621  (ATCC)

Journal: PLoS ONE

Article Title: Mild Alkalization Acutely Triggers the Warburg Effect by Enhancing Hexokinase Activity via Voltage-Dependent Anion Channel Binding

doi: 10.1371/journal.pone.0159529

Figure Lengend Snippet: (A,B) Immunoblots of HK-1 and HK-2 protein (top) and quantified band intensities (bottom) in mitochondrial (A) and cytosolic fractions (B) of T47D cells following 50 min incubation in pH 7.8 or 6.2. HK band intensities of mitochondrial and cytosolic fractions were normalized for loading using COX4 and β-actin bands, respectively. Bars are mean ± SD of band intensities (n = 3) relative to cells in pH 6.2.

Article Snippet: Control siRNA (sc-37007), HK-1 siRNA (sc-39044), HK-2 siRNA (sc-35621), and VDAC1 siRNA (sc-42355) were from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Western Blot, Incubation

Journal: PLoS ONE

Article Title: Mild Alkalization Acutely Triggers the Warburg Effect by Enhancing Hexokinase Activity via Voltage-Dependent Anion Channel Binding

doi: 10.1371/journal.pone.0159529

Figure Lengend Snippet: Relative FDG uptake of T47D cells after transfection with specific siRNA against HK-1, HK-2, or VDAC1. Bars are mean ± SD of six samples from two separate experiments. †, p <0.0005; ‡, p <0.0001, compared to cells transfected with respective siRNA and incubated in pH 6.2.

Article Snippet: Control siRNA (sc-37007), HK-1 siRNA (sc-39044), HK-2 siRNA (sc-35621), and VDAC1 siRNA (sc-42355) were from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Transfection, Incubation

Journal: PLoS ONE

Article Title: Mild Alkalization Acutely Triggers the Warburg Effect by Enhancing Hexokinase Activity via Voltage-Dependent Anion Channel Binding

doi: 10.1371/journal.pone.0159529

Figure Lengend Snippet: (A,B) Confocal microscopy images of T47D cells double stained for VDAC and HK-1 (A) or VDAC and HK-2 (B) following incubation at pH 6.2 or 7.8.

Article Snippet: Control siRNA (sc-37007), HK-1 siRNA (sc-39044), HK-2 siRNA (sc-35621), and VDAC1 siRNA (sc-42355) were from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Confocal Microscopy, Staining, Incubation

Journal: PLoS ONE

Article Title: Mild Alkalization Acutely Triggers the Warburg Effect by Enhancing Hexokinase Activity via Voltage-Dependent Anion Channel Binding

doi: 10.1371/journal.pone.0159529

Figure Lengend Snippet: (A) Immunoblots (top) and quantified band intensities (bottom) of free HK-1 and HK-2 protein dissociated from isolated mitochondria into the supernatant following exposure to pH 6.2 or 7.8. (B) Immunoblots and quantified band intensities of HK-1 and HK-2 protein remaining in the mitochondrial pellets following pH exposure as above. Band intensities of HK from the mitochondrial pellet were normalized by COX4 bands. In supernatants, negative COX-4 bands confirmed the absence of mitochondria in the samples. Bars are mean ± SD of band intensities (n = 2) relative to that at pH 6.2 (A) or pH 7.8 (B).

Article Snippet: Control siRNA (sc-37007), HK-1 siRNA (sc-39044), HK-2 siRNA (sc-35621), and VDAC1 siRNA (sc-42355) were from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Western Blot, Isolation

Journal: PLoS ONE

Article Title: Mild Alkalization Acutely Triggers the Warburg Effect by Enhancing Hexokinase Activity via Voltage-Dependent Anion Channel Binding

doi: 10.1371/journal.pone.0159529

Figure Lengend Snippet: (A, B) Immunoblots (top) and quantified band intensities (bottom) of recombinant HK-1 (A) and HK-2 (B) co-immunoprecipitated with purified VDAC protein. Recombinant HK and purified VDAC protein were pre-incubated in pH 6.2 or 7.8 for 50 min. Bars are mean ± SD (n = 3) of band intensities normalized by VDAC bands relative to that at pH 6.2.

Article Snippet: Control siRNA (sc-37007), HK-1 siRNA (sc-39044), HK-2 siRNA (sc-35621), and VDAC1 siRNA (sc-42355) were from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Western Blot, Recombinant, Immunoprecipitation, Purification, Incubation