p nigrescens atcc 35563  (ATCC)

Journal: Nature Communications

Article Title: An injectable hydrogel enhances tissue repair after spinal cord injury by promoting extracellular matrix remodeling

doi: 10.1038/s41467-017-00583-8

Figure Lengend Snippet: Interaction between macrophages and I-5 hydrogel. a Representative images of Nile Red fluorescence in a macrophage cell line. Cultured macrophages were treated with Nile Red (NR) alone or nanoparticles consisting of NR and either CP-2 or I-5. In addition, JNJ7777120, a histamine receptor 4 inhibitor, or mepyramine maleate, a histamine receptor 1 inhibitor, was added to the culture medium 30 min before treatment with I-5 polymer nanoparticles. Scale bar represents 50 µm. b Graph showing quantification of NR fluorescence intensity. *** indicates p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. N = 4 replicate experiments per group. Error bars represent the SEM. c , d Representative images of transverse spinal cord sections from animals injected with CP-2 hydrogel lacking the imidazole group. c Eriochrome cyanine and eosin staining revealed prominent cystic cavities (*) at the center of the lesion ( c ). d FN staining showed a smaller area of FN-rich matrix. Scale bars represent 200 μm. e 3D reconstruction of the spinal cord tissue from an animal injected with CP-2 hydrogel using the Neurolucida software. Scale bar represents 1 mm. f Graph showing the quantification of the cavity volumes. The data set for the I-5 group was the same as the one used in Fig. . ** indicates p < 0.01 by two-tailed Student’s t -test. N = 8 for the I-5 group and N = 5 for the CP-2 group. Error bars represent the SEM

Article Snippet: To determine if the interaction was mediated by histamine receptors, mepyramine maleate (Santa Cruz), an inhibitor of histamine receptor 1 (H1), or JNJ7777120 (Santa Cruz), an inhibitor for histamine receptor 4 (H4), were added at a concentration of 20 µM to cultured macrophage cells 30 min before adding the nanoparticles.

Techniques: Fluorescence, Cell Culture, Injection, Staining, Software, Two Tailed Test

Journal: Scientific Reports

Article Title: Detection of hydrogen cyanide from oral anaerobes by cavity ring down spectroscopy

doi: 10.1038/srep22577

Figure Lengend Snippet: Screening of oral anaerobes for HCN production in vitro.

Article Snippet: Seven strains of oral anaerobes for the screening test were Porphyromonas gingivalis ATCC 33277, Porphyromonas endodontalis ATCC 35406, Prevotella nigrescens ATCC 35563, Prevotella intermedia ATCC 25611, Fusobacterium nucleatum subsp. nucleatum ATCC 25586, Fusobacterium periodonticum ATCC 33693 and Tannerella forsythia ATCC 43037.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: Plasma membrane microdomains regulate TACE-dependent TNFR1 shedding in human endothelial cells

doi: 10.1111/j.1582-4934.2011.01353.x

Figure Lengend Snippet: Effect of histamine receptors on TNFR1. (A) Cells were cultured in the presence or absence of mepyramine as described in Materials and methods prior to histamine treatment for 30 min. Concentrated medium from treated cells were analysed by SDS-PAGE and immunoblotted with TNFR1 antibody. TMPH was used as specific agonist of H1R. NT represent cells stimulated with vehicle alone. Data are representative of three different experiments. (B) Effect of histamine and TMPH on the expression of surface TNFR1. (C) Effect of mepyramine on histamine-induced down-regulation of membrane TNFR1. (D) Inhibition of TMPH-induced down-regulation of membrane bound TNFR1 in the presence of TACE inhibitor, TAPI-0.

Article Snippet: Mouse anti-TNFR1 and H1R were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Cell Culture, SDS Page, Expressing, Inhibition

Journal: Journal of Cellular and Molecular Medicine

Article Title: Plasma membrane microdomains regulate TACE-dependent TNFR1 shedding in human endothelial cells

doi: 10.1111/j.1582-4934.2011.01353.x

Figure Lengend Snippet: Effect of cav-1 knockdown on proteins distribution and transcripts level. (A) Sucrose gradient fractionation was performed to isolate low buoyant fractions enriched in caveolin proteins. Immunoblotting of fractions harvested from the top (low sucrose density) to the bottom (high sucrose density) show that H1R was excluded from caveolin-enriched fractions. (B) In resting cells, TACE appeared to localize both with low and high sucrose density membranes. By contrast, cav-1 down-regulation by RNAi induced displacement of TACE mainly from the caveolin-enriched membranes (lower panels). (C) Cav-1 immunoprecipitates were immunoblotted for TACE to evaluate protein–protein interaction. IgG served as negative control.

Article Snippet: Mouse anti-TNFR1 and H1R were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Fractionation, Western Blot, Negative Control