gca 001890905 1  (ATCC)

Journal: Microbial Biotechnology

Article Title: Lentils protein isolate as a fermenting substrate for the production of bioactive peptides by lactic acid bacteria and neglected yeast species

doi: 10.1111/1751-7915.14387

Figure Lengend Snippet: Cell density of lactic acid bacteria and yeasts (Log CFU g −1 ) and pH variation (panel A), lactic acid (mg g −1 DW) and ethanol (mg g −1 DW) production (B), protein hydrolysis (mg g −1 DW) and peptide release (mg Leu eq. g −1 ) (C) during the fermentation of red lentil protein isolate (RLPI) carried out at 30°C for 8 days. RLPI was fermented with Lactiplantibacillus plantarum LM1.3 (RLPI‐LM1.3), Lacticaseibacillus rhamnosus ATCC53103 (RLPI‐ATCC), Fructilactibacillus sanfranciscensis E10 (RLPI‐E10), Kazachstania unispora KFBY1 (RLPI‐ KFBY1) and Hanseniaspora uvarum SY1 (RLPI‐ SY1). RLPI without microbial inoculum and incubated under the same conditions (RLPI‐Unstarted) was used as the control. Results are shown as the means (±SD) of three biological replicates analysed in triplicate. Data points with different superscript letters (a–m) differ significantly ( p < 0.05).

Article Snippet: The lowest radial growth inhibition compared to the control was observed in RLPI‐ATCC (50 ± 0.85%) and RLPI‐E10 (35.4 ± 1.13%) (Figure , Figure ).

Techniques: Bacteria, Incubation, Control

Journal: Microbial Biotechnology

Article Title: Lentils protein isolate as a fermenting substrate for the production of bioactive peptides by lactic acid bacteria and neglected yeast species

doi: 10.1111/1751-7915.14387

Figure Lengend Snippet: Peptides profile obtained through RP‐FPLC (detector 240 mm) chromatograms during the fermentation of red lentil protein isolate (RLPI) carried out at 30°C for 8 days. RLPI was fermented with Lactiplantibacillus plantarum LM1.3 (RLPI‐LM1.3), Lacticaseibacillus rhamnosus ATCC53103 (RLPI‐ATCC), Fructilactibacillus sanfranciscensis E10 (RLPI‐E10), Kazachstania unispora KFBY1 (RLPI‐KFBY1) and Hanseniaspora uvarum SY1 (RLPI‐SY1). RLPI without microbial inoculum and incubated under the same conditions (RLPI‐Unstarted) was used as the control. In blue, the chromatograms corresponding to time = 2 days; in orange time = 4 days; in purple time = 6 days; in green the chromatograms corresponding to time = 8 days of fermentation. ‘Raw’ reports the chromatogram of the raw unfermented red lentils protein isolate at time t = 0.

Article Snippet: The lowest radial growth inhibition compared to the control was observed in RLPI‐ATCC (50 ± 0.85%) and RLPI‐E10 (35.4 ± 1.13%) (Figure , Figure ).

Techniques: Incubation, Control

Journal: Microbial Biotechnology

Article Title: Lentils protein isolate as a fermenting substrate for the production of bioactive peptides by lactic acid bacteria and neglected yeast species

doi: 10.1111/1751-7915.14387

Figure Lengend Snippet: In vitro determination of radial growth inhibition against Penicillium roqueforti P1 (panel A), ACE‐inhibitory (B) and ABTS scavenging activity (C) of low molecular weight water soluble extracts (LMW‐WSE) obtained from raw red lentils protein isolate (RLPI‐Raw), RLPI without microbial inoculum (RLPI‐Unstarted) and Fermented‐RLPI, which were incubated at 30°C for 8 days. Fermentation was done spontaneously (RLPI‐Unstarted) or with Lactiplantibacillus plantarum LM1.3 (RLPI‐LM1.3), Lacticaseibacillus rhamnosus ATCC53103 (RLPI‐ATCC), Fructilactibacillus sanfranciscensis E10 (RLPI‐E10), Kazachstania unispora KFBY1 (RLPI‐ KFBY1) and Hanseniaspora uvarum SY1 (RLPI‐ SY1). Results are shown as the means (±SD) of three biological replicates analysed in triplicate. Bars with different superscript letters (a–f) differ significantly ( p < 0.05).

Article Snippet: The lowest radial growth inhibition compared to the control was observed in RLPI‐ATCC (50 ± 0.85%) and RLPI‐E10 (35.4 ± 1.13%) (Figure , Figure ).

Techniques: In Vitro, Inhibition, Activity Assay, Molecular Weight, Incubation

Journal: Microbial Biotechnology

Article Title: Lentils protein isolate as a fermenting substrate for the production of bioactive peptides by lactic acid bacteria and neglected yeast species

doi: 10.1111/1751-7915.14387

Figure Lengend Snippet: Peptidomic analyses of low molecular weight water soluble extracts (LMW‐WSE) obtained from raw red lentils protein isolate (RLPI‐Raw), RLPI without inoculum (RLPI‐Unstarted) and Fermented‐RLPI, which were incubated at 30°C for 8 days. Fermentation was done spontaneously (RLPI‐Unstarted) or Lactiplantibacillus plantarum LM1.3 (RLPI‐LM1.3), Lacticaseibacillus rhamnosus ATCC53103 (RLPI‐ATCC), Fructilactibacillus sanfranciscensis E10 (RLPI‐E10), Kazachstania unispora KFBY1 (RLPI‐ KFBY1) and Hanseniaspora uvarum SY1 (RLPI‐ SY1). Total number of different peptides found in each sample (A) and their distribution based on the molecular weight, employing a colour scale that transitions from blue to red to represent the Log abundance of each identified peptide within each sample (B).

Article Snippet: The lowest radial growth inhibition compared to the control was observed in RLPI‐ATCC (50 ± 0.85%) and RLPI‐E10 (35.4 ± 1.13%) (Figure , Figure ).

Techniques: Molecular Weight, Incubation

Journal: Microbial Biotechnology

Article Title: Lentils protein isolate as a fermenting substrate for the production of bioactive peptides by lactic acid bacteria and neglected yeast species

doi: 10.1111/1751-7915.14387

Figure Lengend Snippet: Peptidomic analyses of low molecular weight water soluble extracts (LMW‐WSE) obtained from raw red lentils protein isolate (RLPI‐Raw), RLPI without inoculum (RLPI‐Unstarted) and Fermented‐RLPI, which were incubated at 30°C for 8 days. Fermentation was done spontaneously (RLPI‐Unstarted) or Lactiplantibacillus plantarum LM1.3 (RLPI‐LM1.3), Lacticaseibacillus rhamnosus ATCC53103 (RLPI‐ATCC), Fructilactibacillus sanfranciscensis E10 (RLPI‐E10), Kazachstania unispora KFBY1 (RLPI‐ KFBY1) and Hanseniaspora uvarum SY1 (RLPI‐ SY1). Upset plot of the intersection of samples, sorted by identified BPs sharing 100% sequence homology with known bioactive peptides using BIOPEP UWM database, (dark circles in the matrix indicate sets that are part of the intersection) (A); relative quantification of BPs and distribution in the samples (B, C). The statistical analysis is shown in the Table .

Article Snippet: The lowest radial growth inhibition compared to the control was observed in RLPI‐ATCC (50 ± 0.85%) and RLPI‐E10 (35.4 ± 1.13%) (Figure , Figure ).

Techniques: Molecular Weight, Incubation, Sequencing

Journal: PLoS ONE

Article Title: 5-HT 2C Receptors Localize to Dopamine and GABA Neurons in the Rat Mesoaccumbens Pathway

doi: 10.1371/journal.pone.0020508

Figure Lengend Snippet: [ A ] Representative composite photomicrograph of the middle level of the VTA displaying the overlay of FG (blue), GAD-67-IR (red) and 5-HT 2C R-IR (green). Inset displays the schematic diagram of the middle VTA (shaded area) and surrounding brain areas (see for abbreviations) at bregma -5.64 mm . High magnification images of the boxed region in panel A depict FG labeling [blue; B], GAD-67-IR [red, C], and 5-HT 2C R-IR [green, D], as well as the overlay of images in B, C, and D to demonstrate colocalization [E]. Filled arrows ( ) indicate cells triple-labeled for FG+GAD-67+5-HT 2C R cells, while the open arrows ( ) indicate a cell double-labeled for FG+5-HT 2C R; as noted in the text, cells labeled for FG+GAD-67 alone were not often detected in the area represented by the boxed region. Scale bars = 20 µm. Note: Portions of IP nucleus present in the composite photomicrograph in panel A were removed from the image prior to incorporation into the figure.

Article Snippet: Validation studies conducted by Santa Cruz Biotechnology revealed that the anti-GAD-67 antibody detected the decrease in GAD-67 protein expression induced by GAD-67 siRNA transfection in HeLa cells (C. Maraviglia, Santa Cruz Biotechnology, personal communication).

Techniques: Labeling

Journal: PLoS ONE

Article Title: 5-HT 2C Receptors Localize to Dopamine and GABA Neurons in the Rat Mesoaccumbens Pathway

doi: 10.1371/journal.pone.0020508

Figure Lengend Snippet: Photomicrographs display FG- [blue, A], GAD-67- [red, B] and 5-HT 2C R-labeling [green, C] in series of five sequential images (from left to right) captured using a confocal microscope in the VTA of a rat injected with FG in the NAc shell. Photomicrographs represent images captured at a distance of 1.0 µm apart through the thickness of the brain section. [D] Overlay of images in A-C shows colocalization of GAD-67- and 5-HT 2C R-IR in a FG-labeled cell in the VTA. Scale bars = 10 µm.

Article Snippet: Validation studies conducted by Santa Cruz Biotechnology revealed that the anti-GAD-67 antibody detected the decrease in GAD-67 protein expression induced by GAD-67 siRNA transfection in HeLa cells (C. Maraviglia, Santa Cruz Biotechnology, personal communication).

Techniques: Labeling, Microscopy, Injection

Journal: PLoS ONE

Article Title: 5-HT 2C Receptors Localize to Dopamine and GABA Neurons in the Rat Mesoaccumbens Pathway

doi: 10.1371/journal.pone.0020508

Figure Lengend Snippet: Schematic representation of the location of cells labeled for FG alone (black squares), FG+GAD-67 (blue circles), FG+5-HT 2C R (green triangles) and FG+GAD-67+5-HT 2C R-labeled cells (red stars) in the [A] rostral (∼bregma −5.10 mm), [B] middle (∼bregma −5.69 mm), and [C] caudal (∼bregma −6.26 mm) levels of the VTA . Insets display schematic diagrams depicting the location of VTA (shaded) relative to surrounding brain areas (see for abbreviations) . Data represent the number and distribution of cells counted in one rostral, middle or caudal section from an animal injected with FG in the NAc shell.

Article Snippet: Validation studies conducted by Santa Cruz Biotechnology revealed that the anti-GAD-67 antibody detected the decrease in GAD-67 protein expression induced by GAD-67 siRNA transfection in HeLa cells (C. Maraviglia, Santa Cruz Biotechnology, personal communication).

Techniques: Labeling, Injection

Journal: PLoS ONE

Article Title: 5-HT 2C Receptors Localize to Dopamine and GABA Neurons in the Rat Mesoaccumbens Pathway

doi: 10.1371/journal.pone.0020508

Figure Lengend Snippet: [A] Representative composite photomicrograph of the middle level of the VTA displaying the overlay of FG (blue), TH-IR (green) and GAD-67-IR (red). Inset displays the schematic diagram of the middle VTA (shaded area) and surrounding brain areas (see for abbreviations] at bregma -5.64 mm. . High magnification images of the boxed region in panel A depict FG labeling [blue, B], TH-IR [green, C], and GAD-67-IR [red, D], as well as the overlay of images in B, C, and D to demonstrate colocalization [E]. Filled arrows ( ) indicate a cell triple-labeled for FG+TH+GAD-67, open arrows ( ) indicate a cell double-labeled for FG+TH, solid arrows ( ) indicate a cell double-labeled for FG+GAD-67, and the arrowheads ( ) point to a cell double-labeled for TH+GAD-67 in the absence of FG; Scale bars = 20 µm. Note: Portions of IP nucleus present in the composite photomicrograph in panel A were removed from the image prior to incorporation into the figure.

Article Snippet: Validation studies conducted by Santa Cruz Biotechnology revealed that the anti-GAD-67 antibody detected the decrease in GAD-67 protein expression induced by GAD-67 siRNA transfection in HeLa cells (C. Maraviglia, Santa Cruz Biotechnology, personal communication).

Techniques: Labeling

Journal: PLoS ONE

Article Title: 5-HT 2C Receptors Localize to Dopamine and GABA Neurons in the Rat Mesoaccumbens Pathway

doi: 10.1371/journal.pone.0020508

Figure Lengend Snippet: Schematic representation of the location of cells labeled for FG alone (black squares), FG+TH (blue circles), FG+GAD-67 (green triangles) and FG+TH+GAD-67-labeled cells (red stars) in the [A] rostral (∼bregma −5.14 mm), [B] middle (∼bregma −5.67 mm), and [C] caudal (∼bregma −6.30 mm) levels of the VTA . Insets display schematic diagrams depicting the location of VTA (shaded) relative to surrounding brain areas (see for abbreviations) . Data represent the number and distribution of cells counted in one rostral, middle or caudal section from a animal injected with FG in the NAc shell.

Article Snippet: Validation studies conducted by Santa Cruz Biotechnology revealed that the anti-GAD-67 antibody detected the decrease in GAD-67 protein expression induced by GAD-67 siRNA transfection in HeLa cells (C. Maraviglia, Santa Cruz Biotechnology, personal communication).

Techniques: Labeling, Injection