bacillus thuringiensis  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Rapid and simple detection of Listeria monocytogenes using real closed dumbbell-mediated isothermal amplification

doi: 10.3389/fmicb.2025.1596797

Figure Lengend Snippet: Real-time L. monocytogenes O-CDA assay. Real-time O-CDA reactions were carried out at 61°C for 60 min. (a) Real-time O-CDA for hly gene positive controls ( hly PC), different L. monocytogenes samples, other extracted DNA samples ( Listeria innocua, Escherichia coli , Shigella sonnei, Salmonella typhimurium, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus cereus , Candida albicans , Candida tropicalis , Streptococcus agalactiae, and Bacillus thuringiensis ), and no template control (NTC). (b) Melting curve analysis of the O-CDA products by real-time PCR.

Article Snippet: The specificity of L. monocytogenes CDA assay was further confirmed by DNA samples extracted from standard strains of Listeria innocua (ATCC 33090), Candida albicans (CICC 1965), Candida tropicalis (BNCC 186815), Streptococcus agalactiae (BNCC 185941), Bacillus thuringiensis (BNCC 353357), Escherichia coli (CVCC 1491), Shigella sonnei (CVCC 3926) , Vibrio parahaemolyticus (CGMCC 1.1997), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (CGMCC 1.6750), and Bacillus cereus (CICC 21261), as listed in .

Techniques: Control, Real-time Polymerase Chain Reaction

Journal: Frontiers in Microbiology

Article Title: Rapid and simple detection of Listeria monocytogenes using real closed dumbbell-mediated isothermal amplification

doi: 10.3389/fmicb.2025.1596797

Figure Lengend Snippet: Colorimetric hly -O-CDA assay using HNB. Line 1, positive controls (10 5 copies of templates, sky blue); Line 2, DNA samples extracted from Listeria innocua (violet); Line 3, DNA samples extracted from Vibrio parahaemolyticus (violet); Line 4, DNA samples extracted from Shigella sonnei (violet); Line 5, DNA samples extracted from Salmonella typhimurium (violet); Line 6, DNA samples extracted from Escherichia coli (violet); Line 7, DNA samples extracted from Staphylococcus aureus (violet); Line 8, DNA samples extracted from Bacillus cereus (violet). Line 9, DNA samples extracted from Candida albicans (violet). Line 10, DNA samples extracted from Candida tropicalis (violet). Line 11, DNA samples extracted from Streptococcus agalactiae (violet). Line 12, DNA samples extracted from Bacillus thuringiensis (violet).

Article Snippet: The specificity of L. monocytogenes CDA assay was further confirmed by DNA samples extracted from standard strains of Listeria innocua (ATCC 33090), Candida albicans (CICC 1965), Candida tropicalis (BNCC 186815), Streptococcus agalactiae (BNCC 185941), Bacillus thuringiensis (BNCC 353357), Escherichia coli (CVCC 1491), Shigella sonnei (CVCC 3926) , Vibrio parahaemolyticus (CGMCC 1.1997), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (CGMCC 1.6750), and Bacillus cereus (CICC 21261), as listed in .

Techniques:

Journal: International Journal of Oncology

Article Title: Daunorubicin induces GLI1-dependent apoptosis in colorectal cancer cell lines

doi: 10.3892/ijo.2024.5654

Figure Lengend Snippet: Daunorubicin-induced caspase-dependent apoptosis in HCT116 cells. (A) Daunorubicin decreased the proliferation of HCT116, HT29, SNU283, DLD-1 and HCT8 cells with GI 50 of 0.597, 0.547, 0.6934, 25.55 and 34.93 μ M, respectively. (B) Colony formation assay using HCT116 cells after treatment of daunorubicin (0, 0.5 and 1) and quantitation. (C) Treatment of daunorubicin (0, 0.5 and 1 μ M) for 24 h-induced apoptosis of HCT116 cells in a dose-dependent manner. Quantitation of cell death is plotted on the right. The graph was drawn by combining the B2 and B4 quadrants. (D) Treatment of daunorubicin (0, 0.5 and 1 μ M) for 24 h led to a dose-dependent increase in caspase3/7 activity. (E) HCT116 cells were pretreated with 25 μ M z-VAD-fmk for 30 min and then treated with daunorubicin (0, 0.5 and 1 μ M). Western blotting was used to measure the expression levels of c-PARP, caspase3, caspase9 and caspase8. (F) After GLI1 knockdown using GLI1 siRNA, cell survival induced by daunorubicin was detected. (G) After GLI1 knockdown using GLI1 siRNA, western blotting was used to measure the expression levels of c-PARP, caspase3, caspase9 and caspase8. The data are expressed as the mean of 3 independent experiments. **P<0.005, *** P<0.001 and **** P<0.0001. siRNA, small interfering RNA; c-PARP, cleaved poly (ADP-ribose) polymerase.

Article Snippet: Con siRNA (cat. no. sc-37007), GLI1 siRNA (cat. no. sc-37911), AKT siRNA (cat. no. sc-43609), ERK siRNA (cat. no. sc-29307, sc-35335), PCAF siRNA (cat. no. sc-36198) and β-TrCP siRNA (cat. no. sc-37178) were all purchased from Santa Cruz Biotechnology, Inc (The siRNA sequence is unavailable by Santa Cruz Biotechnology, Inc).

Techniques: Colony Assay, Quantitation Assay, Activity Assay, Western Blot, Expressing, Knockdown, Small Interfering RNA

Journal: International Journal of Oncology

Article Title: Daunorubicin induces GLI1-dependent apoptosis in colorectal cancer cell lines

doi: 10.3892/ijo.2024.5654

Figure Lengend Snippet: Daunorubicin induces p53-mediated apoptosis and GLI1 downregulation in HCT116 cells. (A) After AKT or ERK knockdown using siRNA, cell survival induced by daunorubicin was detected. (B) A phospho-kinase antibody array analysis showed that daunorubicin substantially increased p53 phosphorylation (S15, S46 and S392). The quantitation is depicted in the lower panel. (C) Western blot analysis for apoptosis-related marker proteins following treatment of daunorubicin (0.5 and 1 μ M) for 24 h. (D) Western blotting was used to measure the expression levels of c-PARP, p53, GLI1, p21 and Cyclin D1 in HCT116 and HCT116 p53 knockout cells. (E) Western blot analysis for PCAF and p300 following treatment of daunorubicin (0.5 and 1 μ M) for 24 h. (F) Results of daunorubicin (0.5 and 1 μ M) treatment for 24 h after PCAF knockdown using PCAF siRNA. The data are expressed as the mean of 3 independent experiments. siRNA, small interfering RNA; K/O, knockout; c-PARP, cleaved poly (ADP-ribose) polymerase.

Article Snippet: Con siRNA (cat. no. sc-37007), GLI1 siRNA (cat. no. sc-37911), AKT siRNA (cat. no. sc-43609), ERK siRNA (cat. no. sc-29307, sc-35335), PCAF siRNA (cat. no. sc-36198) and β-TrCP siRNA (cat. no. sc-37178) were all purchased from Santa Cruz Biotechnology, Inc (The siRNA sequence is unavailable by Santa Cruz Biotechnology, Inc).

Techniques: Knockdown, Ab Array, Phospho-proteomics, Quantitation Assay, Western Blot, Marker, Expressing, Knock-Out, Small Interfering RNA

Journal: International Journal of Oncology

Article Title: Daunorubicin induces GLI1-dependent apoptosis in colorectal cancer cell lines

doi: 10.3892/ijo.2024.5654

Figure Lengend Snippet: Daunorubicin promotes GLI1 ubiquitination and proteasomal degradation. (A) HCT116 cells were treated with 2 μ M MG132 or 100 μ M leupeptin. (B) CHX chase assay showed that daunorubicin reduced the stability of GLI1 protein. Quantitation of GLI1 is also plotted. (C and D) Daunorubicin promoted the ubiquitination of GLI1 in HCT-116 cells. Immunoprecipitation using antibodies against each of the three E3 ligases revealed a major enhancement of β-TrCP-GLI1 interaction. (E) Results of daunorubicin (1 μ M) treatment for 24 h after β-TrCP knockdown using β-TrCP siRNA. Immunoprecipitation using GLI1 and β-TrCP antibody. The data are expressed as the mean of 3 independent experiments. ** P<0.005 and *** P<0.001. CHX, cycloheximide; siRNA, small interfering RNA.

Article Snippet: Con siRNA (cat. no. sc-37007), GLI1 siRNA (cat. no. sc-37911), AKT siRNA (cat. no. sc-43609), ERK siRNA (cat. no. sc-29307, sc-35335), PCAF siRNA (cat. no. sc-36198) and β-TrCP siRNA (cat. no. sc-37178) were all purchased from Santa Cruz Biotechnology, Inc (The siRNA sequence is unavailable by Santa Cruz Biotechnology, Inc).

Techniques: Ubiquitin Proteomics, Protein Quantitation, Immunoprecipitation, Knockdown, Small Interfering RNA