l animalis  (ATCC)


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    Structured Review

    ATCC l animalis
    ). Group I, L. delbrueckii ; group II, L. acidophilus complex (including L. acidophilus , L. intestinalis [ASF 360], L. gasseri , and L. johnsonii ); group III, L. casei complex (including L. casei , L. paracasei , and L. rhamnosus ); group IV, L. <t>animalis</t> , L. murinus , L. brevis , L. buchneri , L. hilgardii , L. fermentum , L. plantarum , L. reuteri , L. ruminis , L. salivarius ,and L. vaginalis .
    L Animalis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l animalis/product/ATCC
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    l animalis - by Bioz Stars, 2022-10
    94/100 stars

    Images

    1) Product Images from "Genotypic and Phenotypic Studies of Murine Intestinal Lactobacilli: Species Differences in Mice with and without Colitis"

    Article Title: Genotypic and Phenotypic Studies of Murine Intestinal Lactobacilli: Species Differences in Mice with and without Colitis

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.70.1.558-568.2004

    ). Group I, L. delbrueckii ; group II, L. acidophilus complex (including L. acidophilus , L. intestinalis [ASF 360], L. gasseri , and L. johnsonii ); group III, L. casei complex (including L. casei , L. paracasei , and L. rhamnosus ); group IV, L. animalis , L. murinus , L. brevis , L. buchneri , L. hilgardii , L. fermentum , L. plantarum , L. reuteri , L. ruminis , L. salivarius ,and L. vaginalis .
    Figure Legend Snippet: ). Group I, L. delbrueckii ; group II, L. acidophilus complex (including L. acidophilus , L. intestinalis [ASF 360], L. gasseri , and L. johnsonii ); group III, L. casei complex (including L. casei , L. paracasei , and L. rhamnosus ); group IV, L. animalis , L. murinus , L. brevis , L. buchneri , L. hilgardii , L. fermentum , L. plantarum , L. reuteri , L. ruminis , L. salivarius ,and L. vaginalis .

    Techniques Used:

    2) Product Images from "Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis"

    Article Title: Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    Journal: Science Advances

    doi: 10.1126/sciadv.abg8335

    Proteomic analysis of L. animalis -EVs and L. animalis . ( A ) Summary of tandem mass spectrometry (MS/MS) database search results for L. animalis -EVs and L. animalis . ( B ) Volcano plot showing the numbers of differentially expressed proteins between L. animalis -EVs and L. animalis with the cutoff of P
    Figure Legend Snippet: Proteomic analysis of L. animalis -EVs and L. animalis . ( A ) Summary of tandem mass spectrometry (MS/MS) database search results for L. animalis -EVs and L. animalis . ( B ) Volcano plot showing the numbers of differentially expressed proteins between L. animalis -EVs and L. animalis with the cutoff of P

    Techniques Used: Mass Spectrometry, Tandem Mass Spectroscopy

    L. animalis -EVs enter the femoral head and mitigate GC-induced ONFH. ( A ) Distribution of the DiR-labeled L. animalis -EVs in the femurs, tibias, and femoral heads of mice detected by ex vivo fluorescent imaging after oral administration for 3, 24, and 72 hours. Scale bar, 6 mm. ( B ) Quantification of the fluorescence intensity. n = 3 per group. ( C ) Distribution of the DiO-labeled L. animalis -EVs within the mouse femoral head after oral administration for 3 hours. Scale bars, 50 μm (white) or 20 μm (red). ( D ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis -EVs on the femoral heads of vehicle- or MPS-treated mice. ( E ) Detection of L. animalis -EVs in the mouse femoral heads using the antibodies targeting L. animalis -EVs ( L -EVs Ab). Scale bars, 50 μm (white) or 20 μm (red). ( F ) Quantification of the mean intensity for L -EV + areas. n = 3 per group. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P
    Figure Legend Snippet: L. animalis -EVs enter the femoral head and mitigate GC-induced ONFH. ( A ) Distribution of the DiR-labeled L. animalis -EVs in the femurs, tibias, and femoral heads of mice detected by ex vivo fluorescent imaging after oral administration for 3, 24, and 72 hours. Scale bar, 6 mm. ( B ) Quantification of the fluorescence intensity. n = 3 per group. ( C ) Distribution of the DiO-labeled L. animalis -EVs within the mouse femoral head after oral administration for 3 hours. Scale bars, 50 μm (white) or 20 μm (red). ( D ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis -EVs on the femoral heads of vehicle- or MPS-treated mice. ( E ) Detection of L. animalis -EVs in the mouse femoral heads using the antibodies targeting L. animalis -EVs ( L -EVs Ab). Scale bars, 50 μm (white) or 20 μm (red). ( F ) Quantification of the mean intensity for L -EV + areas. n = 3 per group. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P

    Techniques Used: Labeling, Mouse Assay, Ex Vivo, Imaging, Fluorescence, Staining, TUNEL Assay

    Cohousing with healthy mice rescues the GC-induced loss of L. animalis , and transplantation with L. animalis protects against GC-induced ONFH. ( A ) Observed number of OTUs and estimated OTU richness (Chao1 and ACE) in fecal microbiota from the cohoused (Ch) or non-cohoused (Non-Ch) vehicle- or MPS-treated mice. n = 3 per group. ( B ) Relative and absolute abundance of the identified fecal microbiota at the genus level tested by 16 S rRNA gene sequencing. n = 3 per group. ( C and D ) Relative and absolute abundance of the genus Lactobacillus (C) and the species L. animalis and L. intestinalis (D) in fecal microbiota from mice in (A). n = 3 per group. ( E ) qRT-PCR analysis of L. animalis abundance in fecal microbiota from mice receiving vehicle or MPS for 1, 2, and 3 weeks. n = 5 per group. ( F ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis on the femoral heads of vehicle- or MPS-treated mice. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P
    Figure Legend Snippet: Cohousing with healthy mice rescues the GC-induced loss of L. animalis , and transplantation with L. animalis protects against GC-induced ONFH. ( A ) Observed number of OTUs and estimated OTU richness (Chao1 and ACE) in fecal microbiota from the cohoused (Ch) or non-cohoused (Non-Ch) vehicle- or MPS-treated mice. n = 3 per group. ( B ) Relative and absolute abundance of the identified fecal microbiota at the genus level tested by 16 S rRNA gene sequencing. n = 3 per group. ( C and D ) Relative and absolute abundance of the genus Lactobacillus (C) and the species L. animalis and L. intestinalis (D) in fecal microbiota from mice in (A). n = 3 per group. ( E ) qRT-PCR analysis of L. animalis abundance in fecal microbiota from mice receiving vehicle or MPS for 1, 2, and 3 weeks. n = 5 per group. ( F ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis on the femoral heads of vehicle- or MPS-treated mice. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P

    Techniques Used: Mouse Assay, Transplantation Assay, Sequencing, Quantitative RT-PCR, Staining, TUNEL Assay

    L. animalis -EVs directly promote angiogenesis, augment osteogenesis, and reduce cell apoptosis. ( A ) Morphology of L. animalis -EVs under a transmission electron microscope. Scale bar, 50 nm. ( B ) Diameter measurement of L. animalis -EVs by nanoparticle tracking analysis. ( C ) Quantification of vesicle numbers in 100 μg of L. animalis -EVs from five different batches (E1, E2, E3, E4, and E5) by nanoparticle tracking analysis. n = 3 per group. ( D ) Uptake of the DiO (green)– or DiI (red)–labeled L. animalis -EVs by HMECs, MLO-Y4, mouse preosteoblast MC3T3-E1 cells, and BMSCs. Scale bar, 10 μm. ( E ) Tube formation images of HMECs treated with vehicle, MPS, or MPS + different concentrations of L. animalis -EVs ( L -EVs). Scale bar, 200 μm. ( F and G ) Quantification of total loops (F) and total tube length (G). n = 3 per group. ( H ) Tube formation images of HMECs treated with vehicle, vehicle + L. animalis -EVs, vehicle + L. reuteri -EVs, MPS, MPS + L. animalis -EVs, or MPS + L. reuteri -EVs. Scale bar, 200 μm. ( I and J ) Quantification of total loops (I) and total tube length (J). n = 3 per group. ( K ) Alizarin red S (ARS) staining images of BMSCs with different treatments under osteogenic induction. Scale bar, 200 μm. ( L ) Quantification of the percentage of ARS + areas. n = 3 per group. ( M to P ) Cell counting kit-8 (CCK-8) analysis of HMECs (M), MLO-Y4 (N), MC3T3-E1 (O), and BMSCs (P) with different treatments. n = 3 per group. ( Q and R ) TUNEL staining images of different cell types receiving different treatments (Q) and quantification of the ratio of TUNEL + apoptotic cells (R). Scale bar, 20 μm. n = 3 per group. * P
    Figure Legend Snippet: L. animalis -EVs directly promote angiogenesis, augment osteogenesis, and reduce cell apoptosis. ( A ) Morphology of L. animalis -EVs under a transmission electron microscope. Scale bar, 50 nm. ( B ) Diameter measurement of L. animalis -EVs by nanoparticle tracking analysis. ( C ) Quantification of vesicle numbers in 100 μg of L. animalis -EVs from five different batches (E1, E2, E3, E4, and E5) by nanoparticle tracking analysis. n = 3 per group. ( D ) Uptake of the DiO (green)– or DiI (red)–labeled L. animalis -EVs by HMECs, MLO-Y4, mouse preosteoblast MC3T3-E1 cells, and BMSCs. Scale bar, 10 μm. ( E ) Tube formation images of HMECs treated with vehicle, MPS, or MPS + different concentrations of L. animalis -EVs ( L -EVs). Scale bar, 200 μm. ( F and G ) Quantification of total loops (F) and total tube length (G). n = 3 per group. ( H ) Tube formation images of HMECs treated with vehicle, vehicle + L. animalis -EVs, vehicle + L. reuteri -EVs, MPS, MPS + L. animalis -EVs, or MPS + L. reuteri -EVs. Scale bar, 200 μm. ( I and J ) Quantification of total loops (I) and total tube length (J). n = 3 per group. ( K ) Alizarin red S (ARS) staining images of BMSCs with different treatments under osteogenic induction. Scale bar, 200 μm. ( L ) Quantification of the percentage of ARS + areas. n = 3 per group. ( M to P ) Cell counting kit-8 (CCK-8) analysis of HMECs (M), MLO-Y4 (N), MC3T3-E1 (O), and BMSCs (P) with different treatments. n = 3 per group. ( Q and R ) TUNEL staining images of different cell types receiving different treatments (Q) and quantification of the ratio of TUNEL + apoptotic cells (R). Scale bar, 20 μm. n = 3 per group. * P

    Techniques Used: Transmission Assay, Microscopy, Labeling, Staining, Cell Counting, CCK-8 Assay, TUNEL Assay

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  • 94
    ATCC l animalis
    ). Group I, L. delbrueckii ; group II, L. acidophilus complex (including L. acidophilus , L. intestinalis [ASF 360], L. gasseri , and L. johnsonii ); group III, L. casei complex (including L. casei , L. paracasei , and L. rhamnosus ); group IV, L. <t>animalis</t> , L. murinus , L. brevis , L. buchneri , L. hilgardii , L. fermentum , L. plantarum , L. reuteri , L. ruminis , L. salivarius ,and L. vaginalis .
    L Animalis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l animalis/product/ATCC
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    l animalis - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    ). Group I, L. delbrueckii ; group II, L. acidophilus complex (including L. acidophilus , L. intestinalis [ASF 360], L. gasseri , and L. johnsonii ); group III, L. casei complex (including L. casei , L. paracasei , and L. rhamnosus ); group IV, L. animalis , L. murinus , L. brevis , L. buchneri , L. hilgardii , L. fermentum , L. plantarum , L. reuteri , L. ruminis , L. salivarius ,and L. vaginalis .

    Journal: Applied and Environmental Microbiology

    Article Title: Genotypic and Phenotypic Studies of Murine Intestinal Lactobacilli: Species Differences in Mice with and without Colitis

    doi: 10.1128/AEM.70.1.558-568.2004

    Figure Lengend Snippet: ). Group I, L. delbrueckii ; group II, L. acidophilus complex (including L. acidophilus , L. intestinalis [ASF 360], L. gasseri , and L. johnsonii ); group III, L. casei complex (including L. casei , L. paracasei , and L. rhamnosus ); group IV, L. animalis , L. murinus , L. brevis , L. buchneri , L. hilgardii , L. fermentum , L. plantarum , L. reuteri , L. ruminis , L. salivarius ,and L. vaginalis .

    Article Snippet: The following 30 reference strains were used: L. acidophilus (ATCC 4356 and ATCC 4796), L. animalis (ATCC 35046), L. brevis subsp. gravesensis (ATCC 27305), L. brevis subsp. otakiensis (ATCC 27306), L. buchneri (ATCC 11577), L. casei (ATCC 334), L. delbrueckii subsp. bulgaricus (ATCC 11842), L. fermentum (ATCC 14931), L. gasseri (ATCC 33323), L. hilgardii (ATCC 8290), L. johnsonii (ATCC 33200), L. murinus (ATCC 35020), L. paracasei (ATCC 25302 and strain Shirota), L. plantarum (ATCC 11581, ATCC 14917, ATCC 49445, and ATCC 4008), L. reuteri (ATCC 23272, ATCC 53608, ATCC 53609, ATCC 55148, and SD2112), L. rhamnosus GG (ATCC 53103), L. ruminis (ATCC 25644), L. salivarius (ATCC 11471), L. vaginalis (ATCC 49540), and Lactobacillus strains ASF 360 and ASF 361.

    Techniques:

    Proteomic analysis of L. animalis -EVs and L. animalis . ( A ) Summary of tandem mass spectrometry (MS/MS) database search results for L. animalis -EVs and L. animalis . ( B ) Volcano plot showing the numbers of differentially expressed proteins between L. animalis -EVs and L. animalis with the cutoff of P

    Journal: Science Advances

    Article Title: Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    doi: 10.1126/sciadv.abg8335

    Figure Lengend Snippet: Proteomic analysis of L. animalis -EVs and L. animalis . ( A ) Summary of tandem mass spectrometry (MS/MS) database search results for L. animalis -EVs and L. animalis . ( B ) Volcano plot showing the numbers of differentially expressed proteins between L. animalis -EVs and L. animalis with the cutoff of P

    Article Snippet: L. animalis ATCC 35046 (BNCC134981; BNBIO, Beijing, China) and L. reuteri ATCC PTA 6475 (ATCC, Manassas, VA, USA) were cultured in de Man Rogosa Sharp (MRS) broth (Solarbio, Beijing, China) with shaking (300 rpm) at 37°C in a microaerophilic chamber (88% N2 , 2% O2 , 5% CO2 , and 5% H2 ; LAI-3T; Shanghai Longyue Instrument Equipment Co. Ltd., Shanghai, China).

    Techniques: Mass Spectrometry, Tandem Mass Spectroscopy

    L. animalis -EVs enter the femoral head and mitigate GC-induced ONFH. ( A ) Distribution of the DiR-labeled L. animalis -EVs in the femurs, tibias, and femoral heads of mice detected by ex vivo fluorescent imaging after oral administration for 3, 24, and 72 hours. Scale bar, 6 mm. ( B ) Quantification of the fluorescence intensity. n = 3 per group. ( C ) Distribution of the DiO-labeled L. animalis -EVs within the mouse femoral head after oral administration for 3 hours. Scale bars, 50 μm (white) or 20 μm (red). ( D ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis -EVs on the femoral heads of vehicle- or MPS-treated mice. ( E ) Detection of L. animalis -EVs in the mouse femoral heads using the antibodies targeting L. animalis -EVs ( L -EVs Ab). Scale bars, 50 μm (white) or 20 μm (red). ( F ) Quantification of the mean intensity for L -EV + areas. n = 3 per group. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P

    Journal: Science Advances

    Article Title: Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    doi: 10.1126/sciadv.abg8335

    Figure Lengend Snippet: L. animalis -EVs enter the femoral head and mitigate GC-induced ONFH. ( A ) Distribution of the DiR-labeled L. animalis -EVs in the femurs, tibias, and femoral heads of mice detected by ex vivo fluorescent imaging after oral administration for 3, 24, and 72 hours. Scale bar, 6 mm. ( B ) Quantification of the fluorescence intensity. n = 3 per group. ( C ) Distribution of the DiO-labeled L. animalis -EVs within the mouse femoral head after oral administration for 3 hours. Scale bars, 50 μm (white) or 20 μm (red). ( D ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis -EVs on the femoral heads of vehicle- or MPS-treated mice. ( E ) Detection of L. animalis -EVs in the mouse femoral heads using the antibodies targeting L. animalis -EVs ( L -EVs Ab). Scale bars, 50 μm (white) or 20 μm (red). ( F ) Quantification of the mean intensity for L -EV + areas. n = 3 per group. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P

    Article Snippet: L. animalis ATCC 35046 (BNCC134981; BNBIO, Beijing, China) and L. reuteri ATCC PTA 6475 (ATCC, Manassas, VA, USA) were cultured in de Man Rogosa Sharp (MRS) broth (Solarbio, Beijing, China) with shaking (300 rpm) at 37°C in a microaerophilic chamber (88% N2 , 2% O2 , 5% CO2 , and 5% H2 ; LAI-3T; Shanghai Longyue Instrument Equipment Co. Ltd., Shanghai, China).

    Techniques: Labeling, Mouse Assay, Ex Vivo, Imaging, Fluorescence, Staining, TUNEL Assay

    Cohousing with healthy mice rescues the GC-induced loss of L. animalis , and transplantation with L. animalis protects against GC-induced ONFH. ( A ) Observed number of OTUs and estimated OTU richness (Chao1 and ACE) in fecal microbiota from the cohoused (Ch) or non-cohoused (Non-Ch) vehicle- or MPS-treated mice. n = 3 per group. ( B ) Relative and absolute abundance of the identified fecal microbiota at the genus level tested by 16 S rRNA gene sequencing. n = 3 per group. ( C and D ) Relative and absolute abundance of the genus Lactobacillus (C) and the species L. animalis and L. intestinalis (D) in fecal microbiota from mice in (A). n = 3 per group. ( E ) qRT-PCR analysis of L. animalis abundance in fecal microbiota from mice receiving vehicle or MPS for 1, 2, and 3 weeks. n = 5 per group. ( F ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis on the femoral heads of vehicle- or MPS-treated mice. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P

    Journal: Science Advances

    Article Title: Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    doi: 10.1126/sciadv.abg8335

    Figure Lengend Snippet: Cohousing with healthy mice rescues the GC-induced loss of L. animalis , and transplantation with L. animalis protects against GC-induced ONFH. ( A ) Observed number of OTUs and estimated OTU richness (Chao1 and ACE) in fecal microbiota from the cohoused (Ch) or non-cohoused (Non-Ch) vehicle- or MPS-treated mice. n = 3 per group. ( B ) Relative and absolute abundance of the identified fecal microbiota at the genus level tested by 16 S rRNA gene sequencing. n = 3 per group. ( C and D ) Relative and absolute abundance of the genus Lactobacillus (C) and the species L. animalis and L. intestinalis (D) in fecal microbiota from mice in (A). n = 3 per group. ( E ) qRT-PCR analysis of L. animalis abundance in fecal microbiota from mice receiving vehicle or MPS for 1, 2, and 3 weeks. n = 5 per group. ( F ) Schematic diagram of the experimental design for testing the effects of oral treatment with L. animalis on the femoral heads of vehicle- or MPS-treated mice. ( G to K ) μCT-reconstructed images of femoral heads (G) and quantification of Tb. BV/TV (H), Tb. Th (I), Tb. N (J), and Tb. Sp (K). Scale bar, 1 mm. n = 8 per group. ( L ) H E staining images of femoral heads. Scale bars, 200 μm (blue) or 50 μm (black). ( M to P ) Quantification of total vessel volume (M) and the numbers of CD31 + (N), OCN + (O), and TUNEL + (P) cells in femoral heads. n = 5 per group. * P

    Article Snippet: L. animalis ATCC 35046 (BNCC134981; BNBIO, Beijing, China) and L. reuteri ATCC PTA 6475 (ATCC, Manassas, VA, USA) were cultured in de Man Rogosa Sharp (MRS) broth (Solarbio, Beijing, China) with shaking (300 rpm) at 37°C in a microaerophilic chamber (88% N2 , 2% O2 , 5% CO2 , and 5% H2 ; LAI-3T; Shanghai Longyue Instrument Equipment Co. Ltd., Shanghai, China).

    Techniques: Mouse Assay, Transplantation Assay, Sequencing, Quantitative RT-PCR, Staining, TUNEL Assay

    L. animalis -EVs directly promote angiogenesis, augment osteogenesis, and reduce cell apoptosis. ( A ) Morphology of L. animalis -EVs under a transmission electron microscope. Scale bar, 50 nm. ( B ) Diameter measurement of L. animalis -EVs by nanoparticle tracking analysis. ( C ) Quantification of vesicle numbers in 100 μg of L. animalis -EVs from five different batches (E1, E2, E3, E4, and E5) by nanoparticle tracking analysis. n = 3 per group. ( D ) Uptake of the DiO (green)– or DiI (red)–labeled L. animalis -EVs by HMECs, MLO-Y4, mouse preosteoblast MC3T3-E1 cells, and BMSCs. Scale bar, 10 μm. ( E ) Tube formation images of HMECs treated with vehicle, MPS, or MPS + different concentrations of L. animalis -EVs ( L -EVs). Scale bar, 200 μm. ( F and G ) Quantification of total loops (F) and total tube length (G). n = 3 per group. ( H ) Tube formation images of HMECs treated with vehicle, vehicle + L. animalis -EVs, vehicle + L. reuteri -EVs, MPS, MPS + L. animalis -EVs, or MPS + L. reuteri -EVs. Scale bar, 200 μm. ( I and J ) Quantification of total loops (I) and total tube length (J). n = 3 per group. ( K ) Alizarin red S (ARS) staining images of BMSCs with different treatments under osteogenic induction. Scale bar, 200 μm. ( L ) Quantification of the percentage of ARS + areas. n = 3 per group. ( M to P ) Cell counting kit-8 (CCK-8) analysis of HMECs (M), MLO-Y4 (N), MC3T3-E1 (O), and BMSCs (P) with different treatments. n = 3 per group. ( Q and R ) TUNEL staining images of different cell types receiving different treatments (Q) and quantification of the ratio of TUNEL + apoptotic cells (R). Scale bar, 20 μm. n = 3 per group. * P

    Journal: Science Advances

    Article Title: Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    doi: 10.1126/sciadv.abg8335

    Figure Lengend Snippet: L. animalis -EVs directly promote angiogenesis, augment osteogenesis, and reduce cell apoptosis. ( A ) Morphology of L. animalis -EVs under a transmission electron microscope. Scale bar, 50 nm. ( B ) Diameter measurement of L. animalis -EVs by nanoparticle tracking analysis. ( C ) Quantification of vesicle numbers in 100 μg of L. animalis -EVs from five different batches (E1, E2, E3, E4, and E5) by nanoparticle tracking analysis. n = 3 per group. ( D ) Uptake of the DiO (green)– or DiI (red)–labeled L. animalis -EVs by HMECs, MLO-Y4, mouse preosteoblast MC3T3-E1 cells, and BMSCs. Scale bar, 10 μm. ( E ) Tube formation images of HMECs treated with vehicle, MPS, or MPS + different concentrations of L. animalis -EVs ( L -EVs). Scale bar, 200 μm. ( F and G ) Quantification of total loops (F) and total tube length (G). n = 3 per group. ( H ) Tube formation images of HMECs treated with vehicle, vehicle + L. animalis -EVs, vehicle + L. reuteri -EVs, MPS, MPS + L. animalis -EVs, or MPS + L. reuteri -EVs. Scale bar, 200 μm. ( I and J ) Quantification of total loops (I) and total tube length (J). n = 3 per group. ( K ) Alizarin red S (ARS) staining images of BMSCs with different treatments under osteogenic induction. Scale bar, 200 μm. ( L ) Quantification of the percentage of ARS + areas. n = 3 per group. ( M to P ) Cell counting kit-8 (CCK-8) analysis of HMECs (M), MLO-Y4 (N), MC3T3-E1 (O), and BMSCs (P) with different treatments. n = 3 per group. ( Q and R ) TUNEL staining images of different cell types receiving different treatments (Q) and quantification of the ratio of TUNEL + apoptotic cells (R). Scale bar, 20 μm. n = 3 per group. * P

    Article Snippet: L. animalis ATCC 35046 (BNCC134981; BNBIO, Beijing, China) and L. reuteri ATCC PTA 6475 (ATCC, Manassas, VA, USA) were cultured in de Man Rogosa Sharp (MRS) broth (Solarbio, Beijing, China) with shaking (300 rpm) at 37°C in a microaerophilic chamber (88% N2 , 2% O2 , 5% CO2 , and 5% H2 ; LAI-3T; Shanghai Longyue Instrument Equipment Co. Ltd., Shanghai, China).

    Techniques: Transmission Assay, Microscopy, Labeling, Staining, Cell Counting, CCK-8 Assay, TUNEL Assay