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2a elispot  (ATCC)


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    Structured Review

    ATCC 2a elispot
    2a Elispot, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/34412/Penicillium+ochrochloron+Biourge/pm41086112-221-49-72
    Average 91 stars, based on 2 article reviews
    2a elispot - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    other:

    Article Title: The Complete Genome Sequence and Analysis of the Epsilonproteobacterium Arcobacter butzleri
    Article Snippet: 97-27 E-value = 5.00E-49 Identity = 53.76% 4 gi|47567067|ref|ZP_00237784.1| Bacillus cereus G9241 E-value = 7.00E-49 Identity = 54.91% 5 gi|52140509|ref|YP_086320.1| Bacillus cereus E33L E-value = 1.00E-48 Identity = 54.91% AB1278 1 kdsB gi|78776926|ref|YP_393241.1| Sulfuromonas denitrificans ATCC 33889 E-value = 6.00E-74 Identity = 58.58% 2 gi|152993256|ref|YP_001358977.1| Sulfurovum sp. NBC37-1 E-value = 3.00E-71 Identity = 60.08% 3 gi|34558211|ref|NP_908026.1| Wolinella succinogenes DSM 1740 E-value = 2.00E-65 Identity = 50.21% 4 gi|86152189|ref|ZP_01070401.1| Campylobacter jejuni subsp. jejuni 260.94 E-value = 2.00E-63 Identity = 54.36% 5 gi|157415084|ref|YP_001482340.1| Campylobacter jejuni subsp. jejuni 81116 E-value = 3.00E-63 Identity = 54.36% AB1279 1 gi|34556560|ref|NP_906375.1| Wolinella succinogenes DSM 1740 E-value = 2.00E-87 Identity = 34.41% 2 gi|150017350|ref|YP_001309604.1| Clostridium beijerinckii NCIMB 8052 E-value = 2.00E-51 Identity = 29.76% 3 gi|146311190|ref|YP_001176264.1| Enterobacter sp. 638 E-value = 8.00E-45 Identity = 28.97% 4 gi|78223826|ref|YP_385573.1| Geobacter metallireducens GS-15 E-value = 3.00E-39 Identity = 25.32% AB1280 1 gi|118474771|ref|YP_892027.1| Campylobacter fetus subsp. fetus 82-40 E-value = 4.00E-94 Identity = 72.69% 2 gi|78777347|ref|YP_393662.1| Sulfuromonas denitrificans ATCC 33889 E-value = 4.00E-94 Identity = 71.25% 3 gi|157164499|ref|YP_001466828.1| Campylobacter concisus 13826 E-value = 9.00E-94 Identity = 71.25% 4 gi|154174132|ref|YP_001408320.1| Campylobacter curvus 525.92 E-value = 6.00E-92 Identity = 70.00% 5 gi|34558382|ref|NP_908197.1| Wolinella succinogenes DSM 1740 E-value = 1.00E-88 Identity = 67.65% AB1281 1 gi|78777348|ref|YP_393663.1| Sulfuromonas denitrificans ATCC 33889 E-value = 4.00E-28 Identity = 50.37% 2 gi|118475620|ref|YP_892026.1| Campylobacter fetus subsp. fetus 82-40 E-value = 3.00E-24 Identity = 46.72% 3 gi|109947486|ref|YP_664714.1| Helicobacter acinonychis str.

    Isolation:

    Article Title: Effects of different culture conditions on biological potential and metabolites production in three Penicillium isolates.
    Article Snippet: .. The molds used in this study were P. ochrochloron Biourge (ATCC 9112), Penicillium funiculosum Thom (ATCC 11797) and P. verrucosum var. cyclopium Westling (Pvc-DS-11) isolated from spoiled cheese by Reis et al20. ..

    cDNA Synthesis:

    Article Title: CircRNA vaccine encoding a chimeric immunogen of B6 and M1 demonstrates robust immune responses against MPXV.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-His-tag mAb-HRP-DirecT MBL Cat# D291-7; RRID: AB_10694870 StrepTag II Mouse Monoclonal Antibody EASYBIO Cat# BE2076; RRID: AB_2801290 Goat Anti-Mouse IgG (H&L)-HRP Conjugated EASYBIO Cat# BE0102; RRID: AB_2923205 Bacterial and virus strains DH5α Chemically Competent Cell TSINGKE Cat# TSC-C14 BL21 (DE3) Chemically Competent Cell TSINGKE Cat# TSV-A09 VACV-WR Prof. M. Fang N/A VACV-TT Prof. W. Tan N/A MPXV Prof. H. Yao GenBase: C_AA040049.1 Chemicals, peptides, and recombinant proteins FastDigest EcoR I Thermo Scientific TM Cat# FD0274 FastDigest EcoR V Thermo Scientific TM Cat# FD0304 FastDigest Hind III Thermo Scientific TM Cat# FD0504 2 × TransStart® FastPfu Fly PCR SuperMix (-dye) TransGen Cat# AS231 GelStain TransGen Cat# GS101-02 Phenol:Cholorform:Isoamyl Alcohol (25:24:1) Merck Cat# P3803 3 M Sodium acetate, pH 5.2 Solarbio Cat# A1070 Recombinant DNase I (RNase-free) TaKaRa Cat# 2270A Recombinant RNase Inhibitor TaKaRa Cat# 2313A RNase-free Water TaKaRa Cat# 9012 GTP (100mM, Nuclease free) Beyotime Cat# D7380 T4 RNA Ligase Reaction Buffer New England Biolabs Cat# B0216S RNase R Vazyme Cat# DD4121-C1 Lipofectamine TM MessengerMAX TM Transfection Reagent ThermoFisher Scientific TM Cat# LMRNA008 ELISA Coating Buffer, 1 × Solarbio Cat# C1050 TMB Chromogen Solution Beyotime Cat# P0209 ELISA Stop Solution Solarbio Cat# C1058 SMM 293-TII Expression Medium (Serum free, complete medium) SinoBiological Cat# M293TII SMS 293-SUPI Expression Medium Supplement SinoBiological Cat# M293-SUPI DMEM, high glucose, pyruvate Gibco TM Cat# 11995065 RPMI 1640 Medium Gibco TM Cat# 11875093 Fetal Bovine Serum (FBS) Gibco TM Cat# A5256501 PageRuler TM Prestained Protein Ladder, 10 to 180 kDa ThermoFisher Scientific TM Cat# 26616 ssRNA Ladder New England Biolabs Cat# N0362S RNA Loading Dye, (2 ×) New England Biolabs Cat# B0363S Critical commercial assays EndoFree Mini Plasmid Kit II TianGen Cat# DP118 Universal DNA Purification Kit TianGen Cat# DP214 HiScribe TM T7 High Yield RNA Synthesis Kit New England Biolabs Cat# E2040S RNAclean Kit TianGen Cat# DP412 (Continued on next page) 14 Cell Reports 44, 116432, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymoclean TM Gel RNA Recovery Kit Zymo Research Cat# R1011 TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat# AT311-02 BeyoGel TM TBE-Urea PAGE Beyotime Cat# R0231S ELISpot Flex: Mouse IFN-γ (ALP) MabTech Cat# 3321-2A ELISpot Flex: Mouse IL-2 (ALP) MabTech Cat# 3441-2A ELISpot Flex: Mouse IL-4 (ALP) MabTech Cat# 3311-2A Experimental models: Cell lines Expi293F cells Gibco TM Cat# A14527; RRID: CVCL_D615 Vero cells ATCC Cat# CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains BALB/c Vital River Cat# 01011; RRID: MGI:2161072 Oligonucleotides SJ-EGFP.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCT GCTGCCCGACAACCACTA 3 ′ Sangon N/A SJ-B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAG TGAATTCTATTCTGCCTACCTGCGTGC 3 ′ Sangon N/A SJ-M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCAG AATGTGATCATCGACGAGTGC 3 ′ Sangon N/A SJ-A35.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC TGCCTAACAAGTCCGACGTG 3 ′ Sangon N/A SJ-A29.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCG CTGGAGAATCACGCAGAGAC 3 ′ Sangon N/A SJ-B6M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGA TCATCGATGAGTGTTATGGAGC 3 ′ Sangon N/A SJ-M1B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC AAGTGGAACCCTATTCTGCCTA 3 ′ Sangon N/A SJ-V3-CVB3.R: 5 ′ CACAGGAAACAGCTATGACCATGATTACGCCAAGCTT CAGTCCGGGGTAACAGAAGTG 3 ′ Sangon N/A Software and algorithms GraphPad Prism 8.0 Graphpad RRID: SCR_002798 BioRender https://www.biorender.com/ RRID: SCR_018361 RNAfold http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/ RNAfold.cgi RRID: SCR_024427 AlphaFold2 https://alphafold.ebi.ac.uk/ RRID: SCR_025454 PyMOL v.2.5.2 https://pymol.org/2/ RRID: SCR_000305 BIAevaluation v4.1 GE Healthcare RRID: SCR_015936 Immuno Capture 6.5.0 Cellular Technology Limited (CTL) N/A Cell Reports 44, 116432, October 28, 2025 15 .. Expi293F cells (Gibco TM ) were cultured in SMM 293-TII medium in a shaking incubator.

    Polyacrylamide Gel Electrophoresis:

    Article Title: CircRNA vaccine encoding a chimeric immunogen of B6 and M1 demonstrates robust immune responses against MPXV.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-His-tag mAb-HRP-DirecT MBL Cat# D291-7; RRID: AB_10694870 StrepTag II Mouse Monoclonal Antibody EASYBIO Cat# BE2076; RRID: AB_2801290 Goat Anti-Mouse IgG (H&L)-HRP Conjugated EASYBIO Cat# BE0102; RRID: AB_2923205 Bacterial and virus strains DH5α Chemically Competent Cell TSINGKE Cat# TSC-C14 BL21 (DE3) Chemically Competent Cell TSINGKE Cat# TSV-A09 VACV-WR Prof. M. Fang N/A VACV-TT Prof. W. Tan N/A MPXV Prof. H. Yao GenBase: C_AA040049.1 Chemicals, peptides, and recombinant proteins FastDigest EcoR I Thermo Scientific TM Cat# FD0274 FastDigest EcoR V Thermo Scientific TM Cat# FD0304 FastDigest Hind III Thermo Scientific TM Cat# FD0504 2 × TransStart® FastPfu Fly PCR SuperMix (-dye) TransGen Cat# AS231 GelStain TransGen Cat# GS101-02 Phenol:Cholorform:Isoamyl Alcohol (25:24:1) Merck Cat# P3803 3 M Sodium acetate, pH 5.2 Solarbio Cat# A1070 Recombinant DNase I (RNase-free) TaKaRa Cat# 2270A Recombinant RNase Inhibitor TaKaRa Cat# 2313A RNase-free Water TaKaRa Cat# 9012 GTP (100mM, Nuclease free) Beyotime Cat# D7380 T4 RNA Ligase Reaction Buffer New England Biolabs Cat# B0216S RNase R Vazyme Cat# DD4121-C1 Lipofectamine TM MessengerMAX TM Transfection Reagent ThermoFisher Scientific TM Cat# LMRNA008 ELISA Coating Buffer, 1 × Solarbio Cat# C1050 TMB Chromogen Solution Beyotime Cat# P0209 ELISA Stop Solution Solarbio Cat# C1058 SMM 293-TII Expression Medium (Serum free, complete medium) SinoBiological Cat# M293TII SMS 293-SUPI Expression Medium Supplement SinoBiological Cat# M293-SUPI DMEM, high glucose, pyruvate Gibco TM Cat# 11995065 RPMI 1640 Medium Gibco TM Cat# 11875093 Fetal Bovine Serum (FBS) Gibco TM Cat# A5256501 PageRuler TM Prestained Protein Ladder, 10 to 180 kDa ThermoFisher Scientific TM Cat# 26616 ssRNA Ladder New England Biolabs Cat# N0362S RNA Loading Dye, (2 ×) New England Biolabs Cat# B0363S Critical commercial assays EndoFree Mini Plasmid Kit II TianGen Cat# DP118 Universal DNA Purification Kit TianGen Cat# DP214 HiScribe TM T7 High Yield RNA Synthesis Kit New England Biolabs Cat# E2040S RNAclean Kit TianGen Cat# DP412 (Continued on next page) 14 Cell Reports 44, 116432, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymoclean TM Gel RNA Recovery Kit Zymo Research Cat# R1011 TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat# AT311-02 BeyoGel TM TBE-Urea PAGE Beyotime Cat# R0231S ELISpot Flex: Mouse IFN-γ (ALP) MabTech Cat# 3321-2A ELISpot Flex: Mouse IL-2 (ALP) MabTech Cat# 3441-2A ELISpot Flex: Mouse IL-4 (ALP) MabTech Cat# 3311-2A Experimental models: Cell lines Expi293F cells Gibco TM Cat# A14527; RRID: CVCL_D615 Vero cells ATCC Cat# CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains BALB/c Vital River Cat# 01011; RRID: MGI:2161072 Oligonucleotides SJ-EGFP.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCT GCTGCCCGACAACCACTA 3 ′ Sangon N/A SJ-B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAG TGAATTCTATTCTGCCTACCTGCGTGC 3 ′ Sangon N/A SJ-M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCAG AATGTGATCATCGACGAGTGC 3 ′ Sangon N/A SJ-A35.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC TGCCTAACAAGTCCGACGTG 3 ′ Sangon N/A SJ-A29.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCG CTGGAGAATCACGCAGAGAC 3 ′ Sangon N/A SJ-B6M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGA TCATCGATGAGTGTTATGGAGC 3 ′ Sangon N/A SJ-M1B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC AAGTGGAACCCTATTCTGCCTA 3 ′ Sangon N/A SJ-V3-CVB3.R: 5 ′ CACAGGAAACAGCTATGACCATGATTACGCCAAGCTT CAGTCCGGGGTAACAGAAGTG 3 ′ Sangon N/A Software and algorithms GraphPad Prism 8.0 Graphpad RRID: SCR_002798 BioRender https://www.biorender.com/ RRID: SCR_018361 RNAfold http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/ RNAfold.cgi RRID: SCR_024427 AlphaFold2 https://alphafold.ebi.ac.uk/ RRID: SCR_025454 PyMOL v.2.5.2 https://pymol.org/2/ RRID: SCR_000305 BIAevaluation v4.1 GE Healthcare RRID: SCR_015936 Immuno Capture 6.5.0 Cellular Technology Limited (CTL) N/A Cell Reports 44, 116432, October 28, 2025 15 .. Expi293F cells (Gibco TM ) were cultured in SMM 293-TII medium in a shaking incubator.

    Enzyme-linked Immunospot:

    Article Title: CircRNA vaccine encoding a chimeric immunogen of B6 and M1 demonstrates robust immune responses against MPXV.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-His-tag mAb-HRP-DirecT MBL Cat# D291-7; RRID: AB_10694870 StrepTag II Mouse Monoclonal Antibody EASYBIO Cat# BE2076; RRID: AB_2801290 Goat Anti-Mouse IgG (H&L)-HRP Conjugated EASYBIO Cat# BE0102; RRID: AB_2923205 Bacterial and virus strains DH5α Chemically Competent Cell TSINGKE Cat# TSC-C14 BL21 (DE3) Chemically Competent Cell TSINGKE Cat# TSV-A09 VACV-WR Prof. M. Fang N/A VACV-TT Prof. W. Tan N/A MPXV Prof. H. Yao GenBase: C_AA040049.1 Chemicals, peptides, and recombinant proteins FastDigest EcoR I Thermo Scientific TM Cat# FD0274 FastDigest EcoR V Thermo Scientific TM Cat# FD0304 FastDigest Hind III Thermo Scientific TM Cat# FD0504 2 × TransStart® FastPfu Fly PCR SuperMix (-dye) TransGen Cat# AS231 GelStain TransGen Cat# GS101-02 Phenol:Cholorform:Isoamyl Alcohol (25:24:1) Merck Cat# P3803 3 M Sodium acetate, pH 5.2 Solarbio Cat# A1070 Recombinant DNase I (RNase-free) TaKaRa Cat# 2270A Recombinant RNase Inhibitor TaKaRa Cat# 2313A RNase-free Water TaKaRa Cat# 9012 GTP (100mM, Nuclease free) Beyotime Cat# D7380 T4 RNA Ligase Reaction Buffer New England Biolabs Cat# B0216S RNase R Vazyme Cat# DD4121-C1 Lipofectamine TM MessengerMAX TM Transfection Reagent ThermoFisher Scientific TM Cat# LMRNA008 ELISA Coating Buffer, 1 × Solarbio Cat# C1050 TMB Chromogen Solution Beyotime Cat# P0209 ELISA Stop Solution Solarbio Cat# C1058 SMM 293-TII Expression Medium (Serum free, complete medium) SinoBiological Cat# M293TII SMS 293-SUPI Expression Medium Supplement SinoBiological Cat# M293-SUPI DMEM, high glucose, pyruvate Gibco TM Cat# 11995065 RPMI 1640 Medium Gibco TM Cat# 11875093 Fetal Bovine Serum (FBS) Gibco TM Cat# A5256501 PageRuler TM Prestained Protein Ladder, 10 to 180 kDa ThermoFisher Scientific TM Cat# 26616 ssRNA Ladder New England Biolabs Cat# N0362S RNA Loading Dye, (2 ×) New England Biolabs Cat# B0363S Critical commercial assays EndoFree Mini Plasmid Kit II TianGen Cat# DP118 Universal DNA Purification Kit TianGen Cat# DP214 HiScribe TM T7 High Yield RNA Synthesis Kit New England Biolabs Cat# E2040S RNAclean Kit TianGen Cat# DP412 (Continued on next page) 14 Cell Reports 44, 116432, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymoclean TM Gel RNA Recovery Kit Zymo Research Cat# R1011 TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat# AT311-02 BeyoGel TM TBE-Urea PAGE Beyotime Cat# R0231S ELISpot Flex: Mouse IFN-γ (ALP) MabTech Cat# 3321-2A ELISpot Flex: Mouse IL-2 (ALP) MabTech Cat# 3441-2A ELISpot Flex: Mouse IL-4 (ALP) MabTech Cat# 3311-2A Experimental models: Cell lines Expi293F cells Gibco TM Cat# A14527; RRID: CVCL_D615 Vero cells ATCC Cat# CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains BALB/c Vital River Cat# 01011; RRID: MGI:2161072 Oligonucleotides SJ-EGFP.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCT GCTGCCCGACAACCACTA 3 ′ Sangon N/A SJ-B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAG TGAATTCTATTCTGCCTACCTGCGTGC 3 ′ Sangon N/A SJ-M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCAG AATGTGATCATCGACGAGTGC 3 ′ Sangon N/A SJ-A35.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC TGCCTAACAAGTCCGACGTG 3 ′ Sangon N/A SJ-A29.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCG CTGGAGAATCACGCAGAGAC 3 ′ Sangon N/A SJ-B6M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGA TCATCGATGAGTGTTATGGAGC 3 ′ Sangon N/A SJ-M1B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC AAGTGGAACCCTATTCTGCCTA 3 ′ Sangon N/A SJ-V3-CVB3.R: 5 ′ CACAGGAAACAGCTATGACCATGATTACGCCAAGCTT CAGTCCGGGGTAACAGAAGTG 3 ′ Sangon N/A Software and algorithms GraphPad Prism 8.0 Graphpad RRID: SCR_002798 BioRender https://www.biorender.com/ RRID: SCR_018361 RNAfold http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/ RNAfold.cgi RRID: SCR_024427 AlphaFold2 https://alphafold.ebi.ac.uk/ RRID: SCR_025454 PyMOL v.2.5.2 https://pymol.org/2/ RRID: SCR_000305 BIAevaluation v4.1 GE Healthcare RRID: SCR_015936 Immuno Capture 6.5.0 Cellular Technology Limited (CTL) N/A Cell Reports 44, 116432, October 28, 2025 15 .. Expi293F cells (Gibco TM ) were cultured in SMM 293-TII medium in a shaking incubator.

    Software:

    Article Title: CircRNA vaccine encoding a chimeric immunogen of B6 and M1 demonstrates robust immune responses against MPXV.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-His-tag mAb-HRP-DirecT MBL Cat# D291-7; RRID: AB_10694870 StrepTag II Mouse Monoclonal Antibody EASYBIO Cat# BE2076; RRID: AB_2801290 Goat Anti-Mouse IgG (H&L)-HRP Conjugated EASYBIO Cat# BE0102; RRID: AB_2923205 Bacterial and virus strains DH5α Chemically Competent Cell TSINGKE Cat# TSC-C14 BL21 (DE3) Chemically Competent Cell TSINGKE Cat# TSV-A09 VACV-WR Prof. M. Fang N/A VACV-TT Prof. W. Tan N/A MPXV Prof. H. Yao GenBase: C_AA040049.1 Chemicals, peptides, and recombinant proteins FastDigest EcoR I Thermo Scientific TM Cat# FD0274 FastDigest EcoR V Thermo Scientific TM Cat# FD0304 FastDigest Hind III Thermo Scientific TM Cat# FD0504 2 × TransStart® FastPfu Fly PCR SuperMix (-dye) TransGen Cat# AS231 GelStain TransGen Cat# GS101-02 Phenol:Cholorform:Isoamyl Alcohol (25:24:1) Merck Cat# P3803 3 M Sodium acetate, pH 5.2 Solarbio Cat# A1070 Recombinant DNase I (RNase-free) TaKaRa Cat# 2270A Recombinant RNase Inhibitor TaKaRa Cat# 2313A RNase-free Water TaKaRa Cat# 9012 GTP (100mM, Nuclease free) Beyotime Cat# D7380 T4 RNA Ligase Reaction Buffer New England Biolabs Cat# B0216S RNase R Vazyme Cat# DD4121-C1 Lipofectamine TM MessengerMAX TM Transfection Reagent ThermoFisher Scientific TM Cat# LMRNA008 ELISA Coating Buffer, 1 × Solarbio Cat# C1050 TMB Chromogen Solution Beyotime Cat# P0209 ELISA Stop Solution Solarbio Cat# C1058 SMM 293-TII Expression Medium (Serum free, complete medium) SinoBiological Cat# M293TII SMS 293-SUPI Expression Medium Supplement SinoBiological Cat# M293-SUPI DMEM, high glucose, pyruvate Gibco TM Cat# 11995065 RPMI 1640 Medium Gibco TM Cat# 11875093 Fetal Bovine Serum (FBS) Gibco TM Cat# A5256501 PageRuler TM Prestained Protein Ladder, 10 to 180 kDa ThermoFisher Scientific TM Cat# 26616 ssRNA Ladder New England Biolabs Cat# N0362S RNA Loading Dye, (2 ×) New England Biolabs Cat# B0363S Critical commercial assays EndoFree Mini Plasmid Kit II TianGen Cat# DP118 Universal DNA Purification Kit TianGen Cat# DP214 HiScribe TM T7 High Yield RNA Synthesis Kit New England Biolabs Cat# E2040S RNAclean Kit TianGen Cat# DP412 (Continued on next page) 14 Cell Reports 44, 116432, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymoclean TM Gel RNA Recovery Kit Zymo Research Cat# R1011 TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat# AT311-02 BeyoGel TM TBE-Urea PAGE Beyotime Cat# R0231S ELISpot Flex: Mouse IFN-γ (ALP) MabTech Cat# 3321-2A ELISpot Flex: Mouse IL-2 (ALP) MabTech Cat# 3441-2A ELISpot Flex: Mouse IL-4 (ALP) MabTech Cat# 3311-2A Experimental models: Cell lines Expi293F cells Gibco TM Cat# A14527; RRID: CVCL_D615 Vero cells ATCC Cat# CCL-81; RRID: CVCL_0059 Experimental models: Organisms/strains BALB/c Vital River Cat# 01011; RRID: MGI:2161072 Oligonucleotides SJ-EGFP.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCT GCTGCCCGACAACCACTA 3 ′ Sangon N/A SJ-B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAG TGAATTCTATTCTGCCTACCTGCGTGC 3 ′ Sangon N/A SJ-M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCAG AATGTGATCATCGACGAGTGC 3 ′ Sangon N/A SJ-A35.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC TGCCTAACAAGTCCGACGTG 3 ′ Sangon N/A SJ-A29.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCG CTGGAGAATCACGCAGAGAC 3 ′ Sangon N/A SJ-B6M1.F: 5 ′ AGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGA TCATCGATGAGTGTTATGGAGC 3 ′ Sangon N/A SJ-M1B6.F: 5 ′ CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCC AAGTGGAACCCTATTCTGCCTA 3 ′ Sangon N/A SJ-V3-CVB3.R: 5 ′ CACAGGAAACAGCTATGACCATGATTACGCCAAGCTT CAGTCCGGGGTAACAGAAGTG 3 ′ Sangon N/A Software and algorithms GraphPad Prism 8.0 Graphpad RRID: SCR_002798 BioRender https://www.biorender.com/ RRID: SCR_018361 RNAfold http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/ RNAfold.cgi RRID: SCR_024427 AlphaFold2 https://alphafold.ebi.ac.uk/ RRID: SCR_025454 PyMOL v.2.5.2 https://pymol.org/2/ RRID: SCR_000305 BIAevaluation v4.1 GE Healthcare RRID: SCR_015936 Immuno Capture 6.5.0 Cellular Technology Limited (CTL) N/A Cell Reports 44, 116432, October 28, 2025 15 .. Expi293F cells (Gibco TM ) were cultured in SMM 293-TII medium in a shaking incubator.



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