custom lna oligonucleotides  (Qiagen)


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    Name:
    Custom LNA Oligonucleotide
    Description:
    Custom LNA Oligonucleotides are ideal for studies involving short or very similar sequences The high affinity of an LNA enhanced oligonucleotide to its complementary sequence results in dramatically improved specificity and sensitivity when compared with traditional DNA or RNA oligos In many cases LNA enhanced oligonucleotides can be used to distinguish between sequences differing by only a single nucleotide a feature that can be critical for the success of many experiments
    Catalog Number:
    339412
    Price:
    None
    Category:
    qPCR Arrays and Assays
    Buy from Supplier


    Structured Review

    Qiagen custom lna oligonucleotides
    Custom LNA Oligonucleotide
    Custom LNA Oligonucleotides are ideal for studies involving short or very similar sequences The high affinity of an LNA enhanced oligonucleotide to its complementary sequence results in dramatically improved specificity and sensitivity when compared with traditional DNA or RNA oligos In many cases LNA enhanced oligonucleotides can be used to distinguish between sequences differing by only a single nucleotide a feature that can be critical for the success of many experiments
    https://www.bioz.com/result/custom lna oligonucleotides/product/Qiagen
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    custom lna oligonucleotides - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark). .. It was purified by HPLC followed by Na + salt exchange and lyophilisation [ ].

    Clone Assay:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark). .. The CTGF luciferase reporter plasmid p(− 1999/+ 36)-Luc (CTGF-luc), based on the backbone of vector pGL3-basic (Promega) [ ], was a gift from Dr. B. Chaqour. c-Rel shRNA sequence (shRel) was obtained from TRC consortium (TRCN0000039986) and cloned, as described previously [ ], in the piggyBac transposon vector (PB-H1-shRNA-GFP), kindly provided by Dr. W.T.

    Transfection:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Paragraph title: Plasmid and transfection ... Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark).

    Article Title: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
    Article Snippet: .. For the target protection experiment, sub-confluent hMADS cells were transfected with TSBs, which are custom designed LNA oligonucleotides with a phosphorothioate backbone (Exiqon); the sequences were 5'-ACATGAAGTAAACACACA-3' for miR-30-TSB and 5'-CAGTCGAAGCTGTTTAC-3' for TSB-neg (mismatch control). .. TSBs were used at a concentration of 20 nM.

    Luciferase:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark). .. The CTGF luciferase reporter plasmid p(− 1999/+ 36)-Luc (CTGF-luc), based on the backbone of vector pGL3-basic (Promega) [ ], was a gift from Dr. B. Chaqour. c-Rel shRNA sequence (shRel) was obtained from TRC consortium (TRCN0000039986) and cloned, as described previously [ ], in the piggyBac transposon vector (PB-H1-shRNA-GFP), kindly provided by Dr. W.T.

    Northern Blot:

    Article Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3
    Article Snippet: .. For Northern blotting, customized LNA oligonucleotides (Exiqon) were used for PCA3 and PRUNE2 ( ). .. For retro-transcription, we designed specific antisense primers for PCA3 or PRUNE2 that allowed generation of specific cDNAs from corresponding pre-mRNA overlapped regions that enabled the generation of strand-specific cDNAs and PCR products for either PCA3 or PRUNE2.

    Negative Control:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Plasmid and transfection The mimic miR-221 (miR-221) and negative control (miR-Ctrl) sequences were purchased from Ambion (Life Technologies, Milan, Italy). .. Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark).

    Article Title: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
    Article Snippet: The negative control was the 'Pre-miR™ miRNA Precursor Molecules - Negative Control #1'. .. For the target protection experiment, sub-confluent hMADS cells were transfected with TSBs, which are custom designed LNA oligonucleotides with a phosphorothioate backbone (Exiqon); the sequences were 5'-ACATGAAGTAAACACACA-3' for miR-30-TSB and 5'-CAGTCGAAGCTGTTTAC-3' for TSB-neg (mismatch control).

    Quantitative RT-PCR:

    Article Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3
    Article Snippet: Paragraph title: qRT-PCR and Northern Blotting. ... For Northern blotting, customized LNA oligonucleotides (Exiqon) were used for PCA3 and PRUNE2 ( ).

    Purification:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark). .. It was purified by HPLC followed by Na + salt exchange and lyophilisation [ ].

    SYBR Green Assay:

    Article Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3
    Article Snippet: qRT-PCR analyses were performed with SYBR-green in a 7500 Fast Real-Time PCR system (Applied Biosystems). .. For Northern blotting, customized LNA oligonucleotides (Exiqon) were used for PCA3 and PRUNE2 ( ).

    Concentration Assay:

    Article Title: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
    Article Snippet: For over-expression, sub-confluent hMADS cells were transfected with Pre-miR™ miRNA precursor molecules (Ambion, Life Technologies Corporation, Carlsbad, CA, USA), at a final concentration of 40 nM. .. For the target protection experiment, sub-confluent hMADS cells were transfected with TSBs, which are custom designed LNA oligonucleotides with a phosphorothioate backbone (Exiqon); the sequences were 5'-ACATGAAGTAAACACACA-3' for miR-30-TSB and 5'-CAGTCGAAGCTGTTTAC-3' for TSB-neg (mismatch control).

    Plasmid Preparation:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Paragraph title: Plasmid and transfection ... Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark).

    Expressing:

    Article Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3
    Article Snippet: Gene expression levels were normalized against the average Ct of three standard endogenous controls (P0 large ribosomal protein, β-glucuronidase, and TATA box-binding protein), and the results were analyzed according to a standard ΔΔCt method. .. For Northern blotting, customized LNA oligonucleotides (Exiqon) were used for PCA3 and PRUNE2 ( ).

    Polymerase Chain Reaction:

    Article Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3
    Article Snippet: For Northern blotting, customized LNA oligonucleotides (Exiqon) were used for PCA3 and PRUNE2 ( ). .. For retro-transcription, we designed specific antisense primers for PCA3 or PRUNE2 that allowed generation of specific cDNAs from corresponding pre-mRNA overlapped regions that enabled the generation of strand-specific cDNAs and PCR products for either PCA3 or PRUNE2.

    Real-time Polymerase Chain Reaction:

    Article Title: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3
    Article Snippet: qRT-PCR analyses were performed with SYBR-green in a 7500 Fast Real-Time PCR system (Applied Biosystems). .. For Northern blotting, customized LNA oligonucleotides (Exiqon) were used for PCA3 and PRUNE2 ( ).

    shRNA:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark). .. The CTGF luciferase reporter plasmid p(− 1999/+ 36)-Luc (CTGF-luc), based on the backbone of vector pGL3-basic (Promega) [ ], was a gift from Dr. B. Chaqour. c-Rel shRNA sequence (shRel) was obtained from TRC consortium (TRCN0000039986) and cloned, as described previously [ ], in the piggyBac transposon vector (PB-H1-shRNA-GFP), kindly provided by Dr. W.T.

    Sequencing:

    Article Title: miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling
    Article Snippet: Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, DenMark). .. LNA-i-miR-221 is a 13-mer DNA/LNA oligonucleotide whose sequence is CAGACAATGTAGC, with a fully PS-modified backbone.

    Article Title: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
    Article Snippet: The sequence for the mismatch control oligonucleotide was CAGTCGAAGCTGTTTAC. .. For the target protection experiment, sub-confluent hMADS cells were transfected with TSBs, which are custom designed LNA oligonucleotides with a phosphorothioate backbone (Exiqon); the sequences were 5'-ACATGAAGTAAACACACA-3' for miR-30-TSB and 5'-CAGTCGAAGCTGTTTAC-3' for TSB-neg (mismatch control).

    Article Title: In Vitro and In Vivo Activity of a Novel Locked Nucleic Acid (LNA)-Inhibitor-miR-221 against Multiple Myeloma Cells
    Article Snippet: Design and Synthesis of LNA Oligonucleotides Custom LNA oligonucleotides were provided by Exiqon (Vedbaek, Denmark). .. LNA-i-miR-221 is a 13-mer DNA/LNA oligonucleotide whose sequence is CAGACAATGTAGC .

    Inhibition:

    Article Title: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
    Article Snippet: For both over-expression and inhibition studies, hMADS cells were submitted to adipogenic medium 3 days after transfection. .. For the target protection experiment, sub-confluent hMADS cells were transfected with TSBs, which are custom designed LNA oligonucleotides with a phosphorothioate backbone (Exiqon); the sequences were 5'-ACATGAAGTAAACACACA-3' for miR-30-TSB and 5'-CAGTCGAAGCTGTTTAC-3' for TSB-neg (mismatch control).

    Over Expression:

    Article Title: Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
    Article Snippet: Paragraph title: Inactivation and over-expression of miRNAs ... For the target protection experiment, sub-confluent hMADS cells were transfected with TSBs, which are custom designed LNA oligonucleotides with a phosphorothioate backbone (Exiqon); the sequences were 5'-ACATGAAGTAAACACACA-3' for miR-30-TSB and 5'-CAGTCGAAGCTGTTTAC-3' for TSB-neg (mismatch control).