mircury lna sybr green pcr kit  (Qiagen)


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    Name:
    miRCURY LNA SYBR Green PCR Kit
    Description:
    For high performance PCR with the miRCURY LNA miRNA PCR System Kit contents miRCURY LNA SYBR Green PCR Kit for miRNA quantification Benefits Optimized for use with miRCURY LNA miRNA PCR Assays and Panels Sensitive and specific detection and quantification of miRNA Includes optional ROX dye for optional normalization of fluorescenc
    Catalog Number:
    339345
    Price:
    172
    Category:
    miRCURY LNA SYBR Green PCR Kits
    Buy from Supplier


    Structured Review

    Qiagen mircury lna sybr green pcr kit
    miRCURY LNA SYBR Green PCR Kit
    For high performance PCR with the miRCURY LNA miRNA PCR System Kit contents miRCURY LNA SYBR Green PCR Kit for miRNA quantification Benefits Optimized for use with miRCURY LNA miRNA PCR Assays and Panels Sensitive and specific detection and quantification of miRNA Includes optional ROX dye for optional normalization of fluorescenc
    https://www.bioz.com/result/mircury lna sybr green pcr kit/product/Qiagen
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    mircury lna sybr green pcr kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation
    Article Snippet: .. For miRNA qPCR assays, cDNA was synthesized from 200 ng of total RNA using the miRCURY LNA Universal RT cDNA synthesis kit (Cat No. 203301; Exiqon/Cat No. 339340; QIAGEN), and expression was assessed using miRCURY LNA SYBR Green (Cat No. 203401; Exiqon/Cat No. 339345; QIAGEN). .. For mRNA qPCR assays, cDNA was synthesized from 500 ng of total RNA using the qScript cDNA Synthesis kit (Cat No. 95047; Quanta/QIAGEN).

    Article Title: microRNA regulation of persistent stress-enhanced memory
    Article Snippet: .. QPCR: A cDNA library was created from 50ng of total RNA using the mirCURY LNA RT Kit (Qiagen, Germantown, MD) and PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen. ..

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Paragraph title: 4.6. Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) ... Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol.

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: .. The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System. .. The expression of miR-21 (Qiagen, Cat# YP00206038), miR-146a (Qiagen, Cat# YP00204688), and miR-16 (Qiagen, Cat# YP00205702) were quantified relative to the expression of the RNA spike-in UniSp2 (Qiagen, Cat# YP00203950).

    Article Title: Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress
    Article Snippet: Paragraph title: QPCR validation ... PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen (formerly Exiqon): mir-135b-3p, YP00204427; mir-199a-5p, YP00204494; mir-29a-5p, YP00204430.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: .. Real-Time PCR assay was performed using miRC URY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The qPCR mixture includes 2x miRCURY SYBR Green Master Mix (5 μl), LNA primer set (1 μl), cDNA template (3 μl) and nuclease-free water up to 10 μl total volume. qPCR thermocycling conditions were as follows: 95°C for 2 min, followed by 40 cycles of 95°C for 10 s, 56°C for 1 min, melt curve analysis were performed between 60–95°C for 15 min at a ramp-rate of 1.6°C/s.

    Article Title: Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
    Article Snippet: .. For analyses of mature miRNA expression, cDNA was generated and qPCR was performed using the miRCURY LNA RT kit (339340; Qiagen), the miRCURY LNA SYBR Green PCR kit (339345; Qiagen), and the following commercially available primers: SNORD48 (YP00203903; Qiagen), hsa-miR-192-5p (YP00204099; Qiagen, Hilden, Germany), and hsa-miR-215-5p (YP00204398; Qiagen). .. Expression was normalized to GAPDH for mRNAs and SNORD48 for miRNAs using the ΔΔCt method.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: .. Real-Time PCR assay was performed using miRCURY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The qPCR mixture includes 2x miRCURY SYBR Green Master Mix (5 μl), LNA primer set (1 μl), cDNA template (3 μl) and nuclease-free water up to 10 μl total volume. qPCR thermocycling conditions were as follows: 95°C for 2 min, followed by 40 cycles of 95°C for 10 s, 56°C for 1 min, melt curve analysis were performed between 60–95°C for 15 min at a ramp-rate of 1.6°C/s.

    Article Title: Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Relative expression of miRNAs was analysed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers (both QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. Thermal cycling was performed on a CFX96 Touch Real Time PCR detection system (BioRad, Munich, Germany).

    Article Title: MicroRNA‐204‐5p: A novel candidate urinary biomarker of Xp11.2 translocation renal cell carcinoma, et al. MicroRNA‐204‐5p: A novel candidate urinary biomarker of Xp11.2 translocation renal cell carcinoma
    Article Snippet: .. For real‐time PCR analysis of miRNAs, PCR was carried out using a miRCURY LNA SYBR Green PCR Kit (Exiqon; Qiagen) and miRCURY LNA Universal RT microRNA PCR Primers (Exiqon; Qiagen) (Table ). ..

    Article Title: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
    Article Snippet: .. Real-time PCR was performed with the QuantStudio 12K Flex Real-Time PCR System (Life Technologies) using either: (A) miRCURY LNA SYBR Green PCR kit (Exiqon) and either miRNA-specific or custom-sequence specific LNA probes (Exiqon) or (B) TaqMan miRNA-specific probes. ..

    Article Title: Biomarker Potential of Plasma MicroRNA-150-5p in Prostate Cancer
    Article Snippet: Plasma miRNome Screening We profiled a set of 752 mature miRNAs using the miRCURY™ LNA™ SYBR® Green PCR Kit (Exiqon, QIAGEN, Vedbaek, Denmark) and miRCURY™ LNA™ miRNA miRNome, Human panel I+II, V3.M (Exiqon, QIAGEN, Vedbaek, Denmark). .. PCR analysis of multiple miRNAs was simultaneously performed on a 7900 HT Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA).

    RNA Extraction:

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: Paragraph title: RNA extraction and qRT-PCR ... The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System.

    Article Title: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
    Article Snippet: After removal of beads, subsequent steps for liver RNA extraction were followed according to manufacturer’s protocol. .. Real-time PCR was performed with the QuantStudio 12K Flex Real-Time PCR System (Life Technologies) using either: (A) miRCURY LNA SYBR Green PCR kit (Exiqon) and either miRNA-specific or custom-sequence specific LNA probes (Exiqon) or (B) TaqMan miRNA-specific probes.

    Amplification:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol. .. The thermal cycler was programmed for initial preheating at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 10 s and 56 °C for 60 s followed by melting curve analysis.

    Article Title: Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress
    Article Snippet: PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen (formerly Exiqon): mir-135b-3p, YP00204427; mir-199a-5p, YP00204494; mir-29a-5p, YP00204430. .. PCR cycle conditions were 95°C for ten minutes then 40 amplification cycles of 95°C for 10 seconds to denature and 60°C for 1 minute to anneal and elongate.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: Reverse transcription (RT) reaction for miRNA assay was performed using miRCURY LNA Universal RT microRNA PCR Kit (Catalog no. 339340; Exiqon-Qiagen, Denmark), the RT was carried out as instructed in miRCURY LNA RT Kit manual, an equal amount of RNA spike-in (Uni-Sp6) was added into RNA and later used as internal extraction and amplification control. .. Real-Time PCR assay was performed using miRC URY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher).

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: Reverse transcription and quantitative real-time PCR (qPCR) Reverse transcription (RT) reaction for miRNA assay was performed using miRCURY LNA Universal RT microRNA PCR Kit (Catalog no. 339340; Exiqon-Qiagen, Denmark), the RT was carried out as instructed in miRCURY LNA RT Kit manual, an equal amount of RNA spike-in (Uni-Sp6) was added into RNA and later used as internal extraction and amplification control. .. Real-Time PCR assay was performed using miRCURY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher).

    RNA Sequencing Assay:

    Article Title: Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress
    Article Snippet: PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen (formerly Exiqon): mir-135b-3p, YP00204427; mir-199a-5p, YP00204494; mir-29a-5p, YP00204430. .. For validation of RNA-seq, cDNA synthesis and qPCR were performed as previously described[ ] with 50ng of RNA using the following Taqman Assays (Thermo-Life Scientific): Chm, Mm00517015_m1; Dnajb4, Mm00508908_m1; Gabrb2, Mm00433467_m1; Rpl15, Mm01727317_g1; Slc8a1, Mm01232254_m1; Ube2d3, Mm00787086_s1.

    Synthesized:

    Article Title: Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation
    Article Snippet: .. For miRNA qPCR assays, cDNA was synthesized from 200 ng of total RNA using the miRCURY LNA Universal RT cDNA synthesis kit (Cat No. 203301; Exiqon/Cat No. 339340; QIAGEN), and expression was assessed using miRCURY LNA SYBR Green (Cat No. 203401; Exiqon/Cat No. 339345; QIAGEN). .. For mRNA qPCR assays, cDNA was synthesized from 500 ng of total RNA using the qScript cDNA Synthesis kit (Cat No. 95047; Quanta/QIAGEN).

    Isolation:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Paragraph title: 4.6. Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) ... Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol.

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: An equal volume of isolated RNA was converted to cDNA using the miRCURY LNA Universal RT microRNA PCR (Qiagen). .. The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System.

    Article Title: Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
    Article Snippet: Paragraph title: RNA Isolation and qPCR ... For analyses of mature miRNA expression, cDNA was generated and qPCR was performed using the miRCURY LNA RT kit (339340; Qiagen), the miRCURY LNA SYBR Green PCR kit (339345; Qiagen), and the following commercially available primers: SNORD48 (YP00203903; Qiagen), hsa-miR-192-5p (YP00204099; Qiagen, Hilden, Germany), and hsa-miR-215-5p (YP00204398; Qiagen).

    Article Title: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
    Article Snippet: Bile, APOB and HDL RNA isolations were processed according to manufacturer’s protocol, except that after addition of ethanol, samples were incubated at −80°C overnight before application to isolation columns and were eluted with a volume of 50 μL. .. Real-time PCR was performed with the QuantStudio 12K Flex Real-Time PCR System (Life Technologies) using either: (A) miRCURY LNA SYBR Green PCR kit (Exiqon) and either miRNA-specific or custom-sequence specific LNA probes (Exiqon) or (B) TaqMan miRNA-specific probes.

    Article Title: Biomarker Potential of Plasma MicroRNA-150-5p in Prostate Cancer
    Article Snippet: Plasma miRNome Screening We profiled a set of 752 mature miRNAs using the miRCURY™ LNA™ SYBR® Green PCR Kit (Exiqon, QIAGEN, Vedbaek, Denmark) and miRCURY™ LNA™ miRNA miRNome, Human panel I+II, V3.M (Exiqon, QIAGEN, Vedbaek, Denmark). .. Total RNA was extracted/purified from 200 µL control (n = 15) and PCa (n = 14) plasma samples using the miRCURY™ RNA Isolation Kit—Biofluids (Exiqon, QIAGEN, Vedbaek, Denmark), according to the manufacturer’s instructions.

    Generated:

    Article Title: Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
    Article Snippet: .. For analyses of mature miRNA expression, cDNA was generated and qPCR was performed using the miRCURY LNA RT kit (339340; Qiagen), the miRCURY LNA SYBR Green PCR kit (339345; Qiagen), and the following commercially available primers: SNORD48 (YP00203903; Qiagen), hsa-miR-192-5p (YP00204099; Qiagen, Hilden, Germany), and hsa-miR-215-5p (YP00204398; Qiagen). .. Expression was normalized to GAPDH for mRNAs and SNORD48 for miRNAs using the ΔΔCt method.

    Quantitative RT-PCR:

    Article Title: Cathelicidin Suppresses Colon Cancer Metastasis via a P2RX7-Dependent Mechanism
    Article Snippet: Paragraph title: Real-Time RT-PCR ... PCR reactions were determined using miRCURY LNA SYBR Green PCR kit (339346, QIAGEN).

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: Paragraph title: 4.6. Total RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) ... Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol.

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: .. The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System. .. The expression of miR-21 (Qiagen, Cat# YP00206038), miR-146a (Qiagen, Cat# YP00204688), and miR-16 (Qiagen, Cat# YP00205702) were quantified relative to the expression of the RNA spike-in UniSp2 (Qiagen, Cat# YP00203950).

    Article Title: YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation
    Article Snippet: .. Quantitative RT-PCR analysis of miRNAs was performed using miRCURY LNA SYBR Green PCR Kit (no. 339346, Qiagen). .. Relative expression was calculated with the 2∆∆Ct method.

    Article Title: Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Relative expression of miRNAs was analysed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers (both QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. Thermal cycling was performed on a CFX96 Touch Real Time PCR detection system (BioRad, Munich, Germany).

    SYBR Green Assay:

    Article Title: Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation
    Article Snippet: .. For miRNA qPCR assays, cDNA was synthesized from 200 ng of total RNA using the miRCURY LNA Universal RT cDNA synthesis kit (Cat No. 203301; Exiqon/Cat No. 339340; QIAGEN), and expression was assessed using miRCURY LNA SYBR Green (Cat No. 203401; Exiqon/Cat No. 339345; QIAGEN). .. For mRNA qPCR assays, cDNA was synthesized from 500 ng of total RNA using the qScript cDNA Synthesis kit (Cat No. 95047; Quanta/QIAGEN).

    Article Title: Cathelicidin Suppresses Colon Cancer Metastasis via a P2RX7-Dependent Mechanism
    Article Snippet: .. PCR reactions were determined using miRCURY LNA SYBR Green PCR kit (339346, QIAGEN). .. Relative miRNA expression was normalized to RNU1A1 expression.

    Article Title: microRNA regulation of persistent stress-enhanced memory
    Article Snippet: .. QPCR: A cDNA library was created from 50ng of total RNA using the mirCURY LNA RT Kit (Qiagen, Germantown, MD) and PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen. ..

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: .. Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol. .. The thermal cycler was programmed for initial preheating at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 10 s and 56 °C for 60 s followed by melting curve analysis.

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: .. The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System. .. The expression of miR-21 (Qiagen, Cat# YP00206038), miR-146a (Qiagen, Cat# YP00204688), and miR-16 (Qiagen, Cat# YP00205702) were quantified relative to the expression of the RNA spike-in UniSp2 (Qiagen, Cat# YP00203950).

    Article Title: Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress
    Article Snippet: .. PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen (formerly Exiqon): mir-135b-3p, YP00204427; mir-199a-5p, YP00204494; mir-29a-5p, YP00204430. .. PCR cycle conditions were 95°C for ten minutes then 40 amplification cycles of 95°C for 10 seconds to denature and 60°C for 1 minute to anneal and elongate.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: .. Real-Time PCR assay was performed using miRC URY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The qPCR mixture includes 2x miRCURY SYBR Green Master Mix (5 μl), LNA primer set (1 μl), cDNA template (3 μl) and nuclease-free water up to 10 μl total volume. qPCR thermocycling conditions were as follows: 95°C for 2 min, followed by 40 cycles of 95°C for 10 s, 56°C for 1 min, melt curve analysis were performed between 60–95°C for 15 min at a ramp-rate of 1.6°C/s.

    Article Title: YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation
    Article Snippet: .. Quantitative RT-PCR analysis of miRNAs was performed using miRCURY LNA SYBR Green PCR Kit (no. 339346, Qiagen). .. Relative expression was calculated with the 2∆∆Ct method.

    Article Title: Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
    Article Snippet: .. For analyses of mature miRNA expression, cDNA was generated and qPCR was performed using the miRCURY LNA RT kit (339340; Qiagen), the miRCURY LNA SYBR Green PCR kit (339345; Qiagen), and the following commercially available primers: SNORD48 (YP00203903; Qiagen), hsa-miR-192-5p (YP00204099; Qiagen, Hilden, Germany), and hsa-miR-215-5p (YP00204398; Qiagen). .. Expression was normalized to GAPDH for mRNAs and SNORD48 for miRNAs using the ΔΔCt method.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: .. Real-Time PCR assay was performed using miRCURY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The qPCR mixture includes 2x miRCURY SYBR Green Master Mix (5 μl), LNA primer set (1 μl), cDNA template (3 μl) and nuclease-free water up to 10 μl total volume. qPCR thermocycling conditions were as follows: 95°C for 2 min, followed by 40 cycles of 95°C for 10 s, 56°C for 1 min, melt curve analysis were performed between 60–95°C for 15 min at a ramp-rate of 1.6°C/s.

    Article Title: Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Relative expression of miRNAs was analysed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers (both QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. Thermal cycling was performed on a CFX96 Touch Real Time PCR detection system (BioRad, Munich, Germany).

    Article Title: MicroRNA‐204‐5p: A novel candidate urinary biomarker of Xp11.2 translocation renal cell carcinoma, et al. MicroRNA‐204‐5p: A novel candidate urinary biomarker of Xp11.2 translocation renal cell carcinoma
    Article Snippet: .. For real‐time PCR analysis of miRNAs, PCR was carried out using a miRCURY LNA SYBR Green PCR Kit (Exiqon; Qiagen) and miRCURY LNA Universal RT microRNA PCR Primers (Exiqon; Qiagen) (Table ). ..

    Article Title: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
    Article Snippet: .. Real-time PCR was performed with the QuantStudio 12K Flex Real-Time PCR System (Life Technologies) using either: (A) miRCURY LNA SYBR Green PCR kit (Exiqon) and either miRNA-specific or custom-sequence specific LNA probes (Exiqon) or (B) TaqMan miRNA-specific probes. ..

    Article Title: Biomarker Potential of Plasma MicroRNA-150-5p in Prostate Cancer
    Article Snippet: .. Plasma miRNome Screening We profiled a set of 752 mature miRNAs using the miRCURY™ LNA™ SYBR® Green PCR Kit (Exiqon, QIAGEN, Vedbaek, Denmark) and miRCURY™ LNA™ miRNA miRNome, Human panel I+II, V3.M (Exiqon, QIAGEN, Vedbaek, Denmark). .. Total RNA was extracted/purified from 200 µL control (n = 15) and PCa (n = 14) plasma samples using the miRCURY™ RNA Isolation Kit—Biofluids (Exiqon, QIAGEN, Vedbaek, Denmark), according to the manufacturer’s instructions.

    Activation Assay:

    Article Title: Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Relative expression of miRNAs was analysed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers (both QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. The cycling conditions comprised initial heat activation at 95 °C for 2 minutes, 40 cycles at 95 °C for 10 seconds and 56 °C for 60 seconds followed by a melt curve analysis. qRT-PCR reactions were performed in duplicates of four biological replicates in every test group.

    Incubation:

    Article Title: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
    Article Snippet: Bile, APOB and HDL RNA isolations were processed according to manufacturer’s protocol, except that after addition of ethanol, samples were incubated at −80°C overnight before application to isolation columns and were eluted with a volume of 50 μL. .. Real-time PCR was performed with the QuantStudio 12K Flex Real-Time PCR System (Life Technologies) using either: (A) miRCURY LNA SYBR Green PCR kit (Exiqon) and either miRNA-specific or custom-sequence specific LNA probes (Exiqon) or (B) TaqMan miRNA-specific probes.

    Polymerase Chain Reaction:

    Article Title: Cathelicidin Suppresses Colon Cancer Metastasis via a P2RX7-Dependent Mechanism
    Article Snippet: .. PCR reactions were determined using miRCURY LNA SYBR Green PCR kit (339346, QIAGEN). .. Relative miRNA expression was normalized to RNU1A1 expression.

    Article Title: microRNA regulation of persistent stress-enhanced memory
    Article Snippet: .. QPCR: A cDNA library was created from 50ng of total RNA using the mirCURY LNA RT Kit (Qiagen, Germantown, MD) and PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen. ..

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: .. Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol. .. The thermal cycler was programmed for initial preheating at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 10 s and 56 °C for 60 s followed by melting curve analysis.

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: .. The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System. .. The expression of miR-21 (Qiagen, Cat# YP00206038), miR-146a (Qiagen, Cat# YP00204688), and miR-16 (Qiagen, Cat# YP00205702) were quantified relative to the expression of the RNA spike-in UniSp2 (Qiagen, Cat# YP00203950).

    Article Title: Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress
    Article Snippet: .. PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen (formerly Exiqon): mir-135b-3p, YP00204427; mir-199a-5p, YP00204494; mir-29a-5p, YP00204430. .. PCR cycle conditions were 95°C for ten minutes then 40 amplification cycles of 95°C for 10 seconds to denature and 60°C for 1 minute to anneal and elongate.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: .. Real-Time PCR assay was performed using miRC URY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The qPCR mixture includes 2x miRCURY SYBR Green Master Mix (5 μl), LNA primer set (1 μl), cDNA template (3 μl) and nuclease-free water up to 10 μl total volume. qPCR thermocycling conditions were as follows: 95°C for 2 min, followed by 40 cycles of 95°C for 10 s, 56°C for 1 min, melt curve analysis were performed between 60–95°C for 15 min at a ramp-rate of 1.6°C/s.

    Article Title: YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation
    Article Snippet: .. Quantitative RT-PCR analysis of miRNAs was performed using miRCURY LNA SYBR Green PCR Kit (no. 339346, Qiagen). .. Relative expression was calculated with the 2∆∆Ct method.

    Article Title: Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
    Article Snippet: .. For analyses of mature miRNA expression, cDNA was generated and qPCR was performed using the miRCURY LNA RT kit (339340; Qiagen), the miRCURY LNA SYBR Green PCR kit (339345; Qiagen), and the following commercially available primers: SNORD48 (YP00203903; Qiagen), hsa-miR-192-5p (YP00204099; Qiagen, Hilden, Germany), and hsa-miR-215-5p (YP00204398; Qiagen). .. Expression was normalized to GAPDH for mRNAs and SNORD48 for miRNAs using the ΔΔCt method.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: .. Real-Time PCR assay was performed using miRCURY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The qPCR mixture includes 2x miRCURY SYBR Green Master Mix (5 μl), LNA primer set (1 μl), cDNA template (3 μl) and nuclease-free water up to 10 μl total volume. qPCR thermocycling conditions were as follows: 95°C for 2 min, followed by 40 cycles of 95°C for 10 s, 56°C for 1 min, melt curve analysis were performed between 60–95°C for 15 min at a ramp-rate of 1.6°C/s.

    Article Title: Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Relative expression of miRNAs was analysed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers (both QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. Thermal cycling was performed on a CFX96 Touch Real Time PCR detection system (BioRad, Munich, Germany).

    Article Title: MicroRNA‐204‐5p: A novel candidate urinary biomarker of Xp11.2 translocation renal cell carcinoma, et al. MicroRNA‐204‐5p: A novel candidate urinary biomarker of Xp11.2 translocation renal cell carcinoma
    Article Snippet: .. For real‐time PCR analysis of miRNAs, PCR was carried out using a miRCURY LNA SYBR Green PCR Kit (Exiqon; Qiagen) and miRCURY LNA Universal RT microRNA PCR Primers (Exiqon; Qiagen) (Table ). ..

    Article Title: Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins
    Article Snippet: .. Real-time PCR was performed with the QuantStudio 12K Flex Real-Time PCR System (Life Technologies) using either: (A) miRCURY LNA SYBR Green PCR kit (Exiqon) and either miRNA-specific or custom-sequence specific LNA probes (Exiqon) or (B) TaqMan miRNA-specific probes. ..

    Article Title: Biomarker Potential of Plasma MicroRNA-150-5p in Prostate Cancer
    Article Snippet: .. Plasma miRNome Screening We profiled a set of 752 mature miRNAs using the miRCURY™ LNA™ SYBR® Green PCR Kit (Exiqon, QIAGEN, Vedbaek, Denmark) and miRCURY™ LNA™ miRNA miRNome, Human panel I+II, V3.M (Exiqon, QIAGEN, Vedbaek, Denmark). .. Total RNA was extracted/purified from 200 µL control (n = 15) and PCa (n = 14) plasma samples using the miRCURY™ RNA Isolation Kit—Biofluids (Exiqon, QIAGEN, Vedbaek, Denmark), according to the manufacturer’s instructions.

    cDNA Library Assay:

    Article Title: microRNA regulation of persistent stress-enhanced memory
    Article Snippet: .. QPCR: A cDNA library was created from 50ng of total RNA using the mirCURY LNA RT Kit (Qiagen, Germantown, MD) and PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen. ..

    Article Title: Bioinformatic analysis of long-lasting transcriptional and translational changes in the basolateral amygdala following acute stress
    Article Snippet: QPCR validation For validation of smRNA-seq, a cDNA library was created from 50ng of total RNA using the mirCURY LNA RT Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol and technical replicate samples that were sequenced. cDNA was diluted 1:80 for PCR. .. PCR reactions were performed in triplicate for each sample using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA) SYBR green primers from Qiagen (formerly Exiqon): mir-135b-3p, YP00204427; mir-199a-5p, YP00204494; mir-29a-5p, YP00204430.

    Expressing:

    Article Title: Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation
    Article Snippet: .. For miRNA qPCR assays, cDNA was synthesized from 200 ng of total RNA using the miRCURY LNA Universal RT cDNA synthesis kit (Cat No. 203301; Exiqon/Cat No. 339340; QIAGEN), and expression was assessed using miRCURY LNA SYBR Green (Cat No. 203401; Exiqon/Cat No. 339345; QIAGEN). .. For mRNA qPCR assays, cDNA was synthesized from 500 ng of total RNA using the qScript cDNA Synthesis kit (Cat No. 95047; Quanta/QIAGEN).

    Article Title: Cathelicidin Suppresses Colon Cancer Metastasis via a P2RX7-Dependent Mechanism
    Article Snippet: Relative mRNA expression was normalized to human 18S or mouse GAPDH mRNA expression. miRNAs were converted to cDNA by miRCURY LNA RT kit (339340, QIAGEN). .. PCR reactions were determined using miRCURY LNA SYBR Green PCR kit (339346, QIAGEN).

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: For miRNA expression analysis, cDNA was synthetized using miRCURY® LNA® RT kit (Qiagen GmbH, Germany) following the manufacturer’s protocol. .. Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol.

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System. .. The expression of miR-21 (Qiagen, Cat# YP00206038), miR-146a (Qiagen, Cat# YP00204688), and miR-16 (Qiagen, Cat# YP00205702) were quantified relative to the expression of the RNA spike-in UniSp2 (Qiagen, Cat# YP00203950).

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: Real-Time PCR assay was performed using miRC URY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The expression levels of target miRNAs were normalized to the RNA spike-in (Uni-Sp6).

    Article Title: YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation
    Article Snippet: Paragraph title: Expression levels of miRNAs by qRT-PCR ... Quantitative RT-PCR analysis of miRNAs was performed using miRCURY LNA SYBR Green PCR Kit (no. 339346, Qiagen).

    Article Title: Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
    Article Snippet: .. For analyses of mature miRNA expression, cDNA was generated and qPCR was performed using the miRCURY LNA RT kit (339340; Qiagen), the miRCURY LNA SYBR Green PCR kit (339345; Qiagen), and the following commercially available primers: SNORD48 (YP00203903; Qiagen), hsa-miR-192-5p (YP00204099; Qiagen, Hilden, Germany), and hsa-miR-215-5p (YP00204398; Qiagen). .. Expression was normalized to GAPDH for mRNAs and SNORD48 for miRNAs using the ΔΔCt method.

    Article Title: The methyltransferase HEN1 is required in Nematostella vectensis for microRNA and piRNA stability as well as larval metamorphosis
    Article Snippet: Real-Time PCR assay was performed using miRCURY SYBR Green PCR Kit (Catalog no. 339345; Exiqon-Qiagen) and the Reactions were performed according to the manufacturer’s instructions using StepOnePlus Real-Time PCR System (ABI instrument, Thermo Fisher). .. The expression levels of target miRNAs were normalized to the RNA spike-in (Uni-Sp6).

    Article Title: Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
    Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Relative expression of miRNAs was analysed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers (both QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. Thermal cycling was performed on a CFX96 Touch Real Time PCR detection system (BioRad, Munich, Germany).

    miRNA RT:

    Article Title: Biomarker Potential of Plasma MicroRNA-150-5p in Prostate Cancer
    Article Snippet: Plasma miRNome Screening We profiled a set of 752 mature miRNAs using the miRCURY™ LNA™ SYBR® Green PCR Kit (Exiqon, QIAGEN, Vedbaek, Denmark) and miRCURY™ LNA™ miRNA miRNome, Human panel I+II, V3.M (Exiqon, QIAGEN, Vedbaek, Denmark). .. The same volume (8 µL) from each eluted RNA was reverse transcribed for cDNA synthesis using the miRCURY™ LNA™ miRNA RT Kit (Exiqon, QIAGEN, Vedbaek, Denmark).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function
    Article Snippet: .. Synthetized cDNA was 15× diluted and used for RT-PCR analysis using miRCURY LNA SYBR® Green PCR kit (Qiagen GmbH, Germany) following the manufacturer’s protocol. .. The thermal cycler was programmed for initial preheating at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 10 s and 56 °C for 60 s followed by melting curve analysis.

    Lysis:

    Article Title: Cushioned-Density Gradient Ultracentrifugation (C-DGUC) improves the isolation efficiency of extracellular vesicles
    Article Snippet: Before the RNA isolation, a mixture of RNA synthetic spike-in templates (Qiagen) composed of UniSp2, UniSp4, UniSp5, and cel-miR-39-3p was added to the lysis buffer for normalization purposes. .. The qRT-PCR was conducted using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on a QuantStudio 7 Flex Real-Time PCR System.

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    Qiagen mircury lna mirna pcr assay
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    Qiagen mircury lna sybr green pcr kit
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