Journal: APL Bioengineering

Article Title: Pulsatile low shear stress increases susceptibility to endothelial inflammation via upregulation of IFT and activation of YAP

doi: 10.1063/5.0263936

Figure Lengend Snippet: Endothelial inflammation caused by LSS is blocked by siRNAs to YAP, MYH10, and IFT88. Cells were treated with siRNAs to either YAP, IFT88, MYH10, or scrambled control for 5 h, followed by a further 4 h pulsatile flow at either HSS or LSS in the presence of TNF-α (20 ng/ml) prior to fixation and analysis. (a) Confocal images of ICAM-1 (green) counterstained for nuclei (blue) and (b) corresponding ICAM-1 integrated intensity normalized to cell number (n = 9 fields). Bars represent mean ± SD with data from three independent experiments. Scale bars = 50 μ m. Statistical analysis was based on a two-way ANOVA with Bonferroni post hoc indicated between LSS and HSS ( * ) and between YAP, IFT88, and MYH10 siRNAs relative to Ctrl siRNA ( # ).

Article Snippet: MYH10 , YAP target gene , sc-376942/mouse , Santa Cruz , 1:100 (WB).

Techniques: Control

Journal: G3: Genes | Genomes | Genetics

Article Title: myh9b is a critical non-muscle myosin II encoding gene that interacts with myh9a and myh10 during zebrafish development in both compensatory and redundant pathways

doi: 10.1093/g3journal/jkae260

Figure Lengend Snippet: NMIIA and NMIIB protein levels in the zebrafish myh mutants. a) Representative western blot of whole larvae extracts at 96 hpf for NMIIA protein with alpha-tubulin control for each of the myh mutants. b) Quantification of NMIIA protein in the myh mutants. c) Representative western blot of whole larvae extracts at 96 hpf for NMIIB protein with alpha-tubulin control for each of the myh mutants. d) Quantification of NMIIB protein in the myh mutants. +/− SEM bars shown for all graphs. N = 3 independent experiments for each protein analyzed. * P < 0.05, ** P < 0.01, and *** P < 0.005.

Article Snippet: Secondary antibodies were diluted in 5% milk/1× TBST and blot was incubated in secondary antibody at room temperature on a shaker for 2 h. Antibodies used include: NMIIA (ab55456, Abcam) at 1:2,000, NMIIB (sc-376942, Santa Cruz Biotechnology) at 1:500, and alpha-tubulin (T6199, Sigma) at 1:2,000 dilutions.

Techniques: Western Blot, Control