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Measuring protein levels <t>of</t> <t>CD56</t> (A) and <t>GZMA</t> (B) in UCB‐MNCs after exposure to RH and PTG Toxoplasma gondii tachyzoites. The results are shown as the mean ± SD of three independent experiments. There was a statistically significant difference in MNC and other groups (* p < .05; ** p < .01, *** p < .001, **** p < .0001). GZMA, granzyme A; UCB‐MNCs, umbilical cord blood mononuclear cells.
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Measuring protein levels of CD56 (A) and GZMA (B) in UCB‐MNCs after exposure to RH and PTG Toxoplasma gondii tachyzoites. The results are shown as the mean ± SD of three independent experiments. There was a statistically significant difference in MNC and other groups (* p < .05; ** p < .01, *** p < .001, **** p < .0001). GZMA, granzyme A; UCB‐MNCs, umbilical cord blood mononuclear cells.

Journal: Immunity, Inflammation and Disease

Article Title: Toxoplasma gondii suppress human cord blood cell differentiation to the NK cell population

doi: 10.1002/iid3.1329

Figure Lengend Snippet: Measuring protein levels of CD56 (A) and GZMA (B) in UCB‐MNCs after exposure to RH and PTG Toxoplasma gondii tachyzoites. The results are shown as the mean ± SD of three independent experiments. There was a statistically significant difference in MNC and other groups (* p < .05; ** p < .01, *** p < .001, **** p < .0001). GZMA, granzyme A; UCB‐MNCs, umbilical cord blood mononuclear cells.

Article Snippet: The membranes were blocked in 2% skim milk dissolved in TBS‐T (20 mM Tris, 137 mM NaCl, and 0.1% Tween 20) for 1 h. The membranes were incubated with primary monoclonal antibodies against β‐actin (dilution: 1:300) and CD56 (dilution: 1:200, Cat no: sc‐7326; Santa Cruz) and GZMA (1:200, Cat no: sc‐33692; Santa Cruz) at 4°C for 18 h. Following three‐time washes in TBS‐T, the membranes were incubated with goat anti‐mouse secondary antibody (dilution: 1:1000, Cat no: sc‐2357; Santa Cruz) for 1 h. Reactive immunoblots were visualized using a chemiluminescence Kit (ECL; GERPN418) and X‐ray film (Fujifilm).

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