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yersinia intermedia  (ATCC)


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    ATCC yersinia intermedia
    Yersinia Intermedia, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yersinia intermedia/product/ATCC
    Average 93 stars, based on 7 article reviews
    yersinia intermedia - by Bioz Stars, 2025-11
    93/100 stars

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    Characterization of NK cell cytotoxicity-related genes in BGC823 and AGS cells. (a) The signaling pathway of NK cell-mediated cytotoxicity. (b) Western blot analysis of NK cell cytotoxicity-related proteins in BGC823 and AGS cells. (c) Western blot analysis of pERK, Bag6 and IL-12 in BGC823 and AGS cells. (d) Western blot analysis of NKp30 and IL-12 when NKp30 was knocked down in AGS cells. (e) Cytotoxicity assay monitoring for NK cell lysis of AGS cells when NKp30 was knocked down (left) or when IL-12 was blocked by a specific antibody (right).

    Journal: Oncogene

    Article Title: Copy number variations of HLA-I and activation of NKp30 pathway determine the sensitivity of gastric cancer cells to the cytotoxicity of natural killer cells

    doi: 10.1038/onc.2015.324

    Figure Lengend Snippet: Characterization of NK cell cytotoxicity-related genes in BGC823 and AGS cells. (a) The signaling pathway of NK cell-mediated cytotoxicity. (b) Western blot analysis of NK cell cytotoxicity-related proteins in BGC823 and AGS cells. (c) Western blot analysis of pERK, Bag6 and IL-12 in BGC823 and AGS cells. (d) Western blot analysis of NKp30 and IL-12 when NKp30 was knocked down in AGS cells. (e) Cytotoxicity assay monitoring for NK cell lysis of AGS cells when NKp30 was knocked down (left) or when IL-12 was blocked by a specific antibody (right).

    Article Snippet: We used antibodies against HLA-A (1913–1; Epitomics, Burlingame, CA, USA), HLA-B (2389–1; Epitomics), HLA-C (5472–1; Epitomics), MICA (T3305; Epitomics), VAV2 (B1241; Anbo, San Francisco, CA, USA), MAPK3 (C11133; Anbo), NKp30 (BS3888; Bioworld, St Louis Park, MN, USA), and pERK (Tyr204) (sc-7383; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bag6 (6763–1; Epitomics) and actin (A5441; Sigma, St Louis, MO, USA).

    Techniques: Western Blot, Cytotoxicity Assay, Lysis

    Expression patterns of HLA-I and the NKp30/VAV2/MAPK3/IL-12 pathway in GC cell lines. (a) qPCR detected relative CNV of HLA-A, B and C in GC cell lines. The value of HLA-I is the sum value of HLA-A, -B and -C. Owing to the copy number of HLA-A, -B was not changed in AGS cells, the relative copy number of HLA-A, -B was compared with AGS cells; for HLA-C, as it was lost in AGS cells, the relative copy number of HLA-C was compared with BGC823 cells. (b) Western blot analysis of NK cell cytotoxicity-related proteins in GC cell lines with high and low tumorigenic capacities.

    Journal: Oncogene

    Article Title: Copy number variations of HLA-I and activation of NKp30 pathway determine the sensitivity of gastric cancer cells to the cytotoxicity of natural killer cells

    doi: 10.1038/onc.2015.324

    Figure Lengend Snippet: Expression patterns of HLA-I and the NKp30/VAV2/MAPK3/IL-12 pathway in GC cell lines. (a) qPCR detected relative CNV of HLA-A, B and C in GC cell lines. The value of HLA-I is the sum value of HLA-A, -B and -C. Owing to the copy number of HLA-A, -B was not changed in AGS cells, the relative copy number of HLA-A, -B was compared with AGS cells; for HLA-C, as it was lost in AGS cells, the relative copy number of HLA-C was compared with BGC823 cells. (b) Western blot analysis of NK cell cytotoxicity-related proteins in GC cell lines with high and low tumorigenic capacities.

    Article Snippet: We used antibodies against HLA-A (1913–1; Epitomics, Burlingame, CA, USA), HLA-B (2389–1; Epitomics), HLA-C (5472–1; Epitomics), MICA (T3305; Epitomics), VAV2 (B1241; Anbo, San Francisco, CA, USA), MAPK3 (C11133; Anbo), NKp30 (BS3888; Bioworld, St Louis Park, MN, USA), and pERK (Tyr204) (sc-7383; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bag6 (6763–1; Epitomics) and actin (A5441; Sigma, St Louis, MO, USA).

    Techniques: Expressing, Western Blot

    Schematic representation of the proposed mechanism of NK cell lysis for cancer cells. Two human GC cell lines, AGS and BGC823, were used in this study. As left shown, AGS cells did not express HLA-C due to the loss of DNA copy, and NK cells were activated because of active NKp30/VAV2/MAPK3/IL-12 pathway in AGS cells. As right shown, BGC823 cells highly expressed HLA-I, an inhibitor of NK cell recognition, due to gene amplification, and NK cells were not activated because of lacking NKp30 in BGC823 cells.

    Journal: Oncogene

    Article Title: Copy number variations of HLA-I and activation of NKp30 pathway determine the sensitivity of gastric cancer cells to the cytotoxicity of natural killer cells

    doi: 10.1038/onc.2015.324

    Figure Lengend Snippet: Schematic representation of the proposed mechanism of NK cell lysis for cancer cells. Two human GC cell lines, AGS and BGC823, were used in this study. As left shown, AGS cells did not express HLA-C due to the loss of DNA copy, and NK cells were activated because of active NKp30/VAV2/MAPK3/IL-12 pathway in AGS cells. As right shown, BGC823 cells highly expressed HLA-I, an inhibitor of NK cell recognition, due to gene amplification, and NK cells were not activated because of lacking NKp30 in BGC823 cells.

    Article Snippet: We used antibodies against HLA-A (1913–1; Epitomics, Burlingame, CA, USA), HLA-B (2389–1; Epitomics), HLA-C (5472–1; Epitomics), MICA (T3305; Epitomics), VAV2 (B1241; Anbo, San Francisco, CA, USA), MAPK3 (C11133; Anbo), NKp30 (BS3888; Bioworld, St Louis Park, MN, USA), and pERK (Tyr204) (sc-7383; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bag6 (6763–1; Epitomics) and actin (A5441; Sigma, St Louis, MO, USA).

    Techniques: Lysis, Amplification